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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for Recombinant Human CD96 Protein T7 His TEV tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... CRISPRScan was used to identify sgRNA sequences with high predicted cut efficiencies for early exons and sgRNAs were synthesized using T7 in vitro transcription with the MEGAshortscriptTM T7 Transcription Kit (AM1354, ThermoFisher). To minimize off target-effects ...
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bioRxiv - Developmental Biology 2021Quote: ... which habors the T7 promoter abided by the gRNA sequence and the sharped gRNA scaffold using MEGAscript T7 Transcription Kit (Invitrogen) according the manufacturer’s protocols ...
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bioRxiv - Biophysics 2019Quote: ... cRNA of each construct was prepared using T7 RNA polymerase (mMESSAGE mMACHINE® T7 Kit; Applied Biosystems/Ambion, Austin, TX) in an in-vitro transcription process ...
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bioRxiv - Biophysics 2019Quote: ... cRNA of each construct was prepared using T7 RNA polymerase (mMESSAGE mMACHINE® T7 Kit; Applied Biosystems/Ambion, Austin, TX) in an in-vitro transcription process ...
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bioRxiv - Cell Biology 2022Quote: ... Complementary RNAs were synthesized from the PCR templates using T3 or T7 RNA polymerases (MEGAscript T3 or T7 kit, ThermoFisher). Single-stranded RNA products were then purified using MEGAclear kit (ThermoFisher ...
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bioRxiv - Biochemistry 2022Quote: ... were used to generate an amplicon of the insert containing T7 RNA polymerase binding sites from which gene-specific dsRNAs were synthesised by in vitro transcription (T7 Megascript, Thermofisher). DNase1-digested dsRNAs were purified by phenol/chloroform extraction ...
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bioRxiv - Microbiology 2023Quote: ... The RNA was used to generate cDNA that was subsequently used in the amplification of CTL4 using primers P51/P52 with T7 overhangs to produce double-stranded RNA using the T7 high yield transcription kit (ThermoFisher). The double-stranded RNA was purified using the RNeasy kit (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... Transcription of RNAs in vitro by T7 RNA polymerase from the DNA templates was then carried out using the MEGAscript T7 kit (Ambion) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: PCR products were used for T7 RNA polymerase-driven transcription in vitro using the MEGAscript T7 kit (Ambion/Thermo Scientific), according to the manufacturer’s instructions and analogous to our previous publication (31) ...
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bioRxiv - Microbiology 2023Quote: PCR products were used for T7 RNA polymerase-driven transcription in vitro using the MEGAscript T7 kit (Ambion/Thermo Scientific), according to the manufacturer’s instructions and analogous to our previous publication (31) ...
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bioRxiv - Molecular Biology 2023Quote: ... with IVT mix (0.5 µL Ribolock inhibitor, Invitrogen, 2 µL T7 polymerase buffer, NEB, 8 µL rNTP mix, 2 µL T7 polymerase, Invitrogen). DNA was removed by addition of 1 µL turbo DNase (Invitrogen ...
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bioRxiv - Genetics 2022Quote: ... This PCR product was added as template to a T7 RNA polymerase transcription reaction using the Megascript T7 kit (Invitrogen). Following 3 h of RNA transcription at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... was synthesized on templates that contain the T7 promoter sequence (5’-TAATACGACTCACTATAGGGTACT-3’) at each end using a MEGAscript T7 kit (Ambion); templates were amplified from 0–4 h embryonic cDNA using specific primers (5’-AGCAGATGACGACGACAACA-3 ...
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bioRxiv - Zoology 2024Quote: ... sgRNA templates were transcribed using a T7 promoter and synthesized in vitro using the MAXIscript T7 kit (Ambion, Austin, USA) according to the manufacture’s instruction ...
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bioRxiv - Molecular Biology 2024Quote: ... was cut with Hind III and used as a template for T7 transcription according to the instructions in the Megaprime T7 transcription kit (Invitrogen) overnight ...
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bioRxiv - Microbiology 2024Quote: In vitro RNA synthesis was done with the T7 RNA polymerase using PCR products as templates and the T7 Megascript kit (Ambion) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: Whole-cell protein extracts were isolated from human cells with conventional RIPA lysis buffer and protein concentration assayed by BCA kit (Thermo Scientific). Blocking was carried out in 10% skim milk in PBST for 1 h ...
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bioRxiv - Biochemistry 2019Quote: Protein concentration in g ⋅ L−1 of human serum was calculated using PierceTM Bicinchoninic Acid (BCA) Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... The Z-scores for reactivity of SARS CoV-2 proteins to each human protein was determined by using ProtoArray Prospector v 5.2 software (Invitrogen, Thermo Fisher).
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bioRxiv - Genetics 2021Quote: ... 12 µL Hi-Di Formamide (Applied Biosystems) and 0.3 µL MapMarker 500 labelled with DY-632 (Eurogentec) ...
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bioRxiv - Microbiology 2019Quote: ... we used Dynabeads™ His-Tag (Invitrogen) for Ni-affinity purification ...
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bioRxiv - Bioengineering 2022Quote: 6x-His tag antibodies (Invitrogen, MA1-21315) were coated at 2 µg/mL in PBS onto 96-well half- area high binding plates (Corning ...
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bioRxiv - Immunology 2021Quote: ... blot using an anti-His Ab (ThermoFisher). MSP-142 and AMA-1 were biotinylated and tetramerized with streptavidin-PE (SA-PE ...
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bioRxiv - Microbiology 2021Quote: ... eluted in Hi-Di formamide (Applied Biosystems) with ROX-350 ladder ...
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bioRxiv - Biochemistry 2021Quote: ... anti-His (MA1-21315, Invitrogen, 1:1,000). Proteins separated by SDS-PAGE were detected following the manufacturer’s guidelines (GE Healthcare ECL Western Blotting detection system ...
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bioRxiv - Microbiology 2020Quote: Hi-sensitivity Qubit DNA kit (Thermo Fisher) was used to measure the DNA concentration directly from the lysate ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% HI FBS (Gibco 10500), 20% D4T conditioned medium (24) ...
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bioRxiv - Microbiology 2020Quote: ... and cloned into pcDNA-V5/His (Invitrogen). The M fragment of CCHFV was PCR amplified with primers shown in Table II from cDNA generated from CCHFV genomic RNA ...
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bioRxiv - Microbiology 2021Quote: ... mouse anti-His (MA1-21315, Thermo Scientific), mouse anti-β-actin HRP (Santa Cruz Biotechnologies ...
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bioRxiv - Biochemistry 2020Quote: ... was loaded onto His Dynabeads (Invitrogen 10103D), relying on the intrinsic metal-binding properties of untagged PrP ...
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bioRxiv - Zoology 2019Quote: ... Primary (rabbit anti-His, Thermo Fisher Scientific) and secondary (anti-rabbit Alexa Fluor 594 ...
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bioRxiv - Synthetic Biology 2020Quote: ... supplemented with 10% HI FBS (Thermo Fisher). 30,000 HEK293AAV cells were added to each well of a 48 well plate one day before transfection ...
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bioRxiv - Cell Biology 2020Quote: ... 6x-His Tag antibody is from ThermoFisher Scientific (MA1-21315-D550) ...
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bioRxiv - Immunology 2020Quote: ... with 10 % V/V Hi FCS (Gibco), 1 mM sodium pyruvate (Gibco ...
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bioRxiv - Biochemistry 2021Quote: ... terbium-coupled anti-his at 1nM (Invitrogen) and decreasing concentrations of compounds in 384-well microplates (Corning ...
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bioRxiv - Biochemistry 2021Quote: ... mouse anti-His Tag (Invitrogen MA1-21315). Secondary antibody used are ...
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bioRxiv - Neuroscience 2021Quote: ... HI Horse Serum (Invitrogen, 2% vol/vol), GlutaMax (2 mM ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 17.4 μl Hi-Di Formamide (Applied Biosystems), and 2 μL of RT reaction mixture were loaded in a 96-well plate ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Anti-His antibody was from Thermo Fisher Scientific (37-2900 ...
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bioRxiv - Microbiology 2022Quote: ... mouse monoclonal anti-6x-His (Thermo Fisher) diluted 1:1,000 ...
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bioRxiv - Cell Biology 2022Quote: ... anti-His tag (ThermoFisher Scientific, MA1-21315), and anti-tubulin (DHSB ...
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bioRxiv - Zoology 2022Quote: ... AeStaufen cDNA synthesized by Genscript was cloned into pIZT/ V5-His (Invitrogen). The same plasmid containing Chloramphenicol acetyltransferase (CAT ...
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bioRxiv - Molecular Biology 2023Quote: ... The antibody against His (631212, Fisher Scientific) is commercially available ...
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bioRxiv - Neuroscience 2023Quote: The plasmid pcDNA6.1/V5-His B (Thermofisher) was used as the template to subclone in the full-length PNPLA6 cDNA (from RefSeq transcript NM_001166111.1) ...
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bioRxiv - Microbiology 2023Quote: ... and probed with □-His mAb (Thermo Scientific) diluted 1:1,000 in 6% milk in PBS-T followed by α-mouse IgG HRP (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... His Pur cobalt spin columns (Thermo Scientific) were used to purify the in vitro-translated proteins ...
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bioRxiv - Immunology 2024Quote: ... Trichoplusia ni (Hi-5, Thermo Fisher Scientific) and Spodoptera fugiperda (Sf9 ...
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bioRxiv - Microbiology 2024Quote: ... 10% HI-FBS (v/v, Gibco 10082147) 100 U/mL penicillin ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant (Invitrogen, no. 10342-020). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Microbiology 2022Quote: Purified recombinant β-lactamase (ThermoFisher) in PBS (0.15 nM ...