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Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for Recombinant Human CD96 Protein T7 His TEV tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... Recombinant CSF1R (Invitrogen, Carlsbad, CA) was used at a concentration of 2.5 nM ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% mouse recombinant EGF (Invitrogen, 5% Recombinant human R-spondin (Peprotech).
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bioRxiv - Cancer Biology 2024Quote: ... recombinant (Thermo Fisher Scientific, #10342020), and specific oligonucleotides (Supplementary Table S4 ...
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bioRxiv - Genetics 2021Quote: ... or the MEGAscript T7 Transcription kit (Thermo Fisher), and purified using the RNA Clean & Concentrator kit (Zymo) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... using the MEGAscript™ T7 Transcription Kit (ThermoFisher). After synthesis ...
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bioRxiv - Cell Biology 2022Quote: ... the Megascript kits for SP6 and T7 (Invitrogen) were used ...
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bioRxiv - Biochemistry 2020Quote: ... A MEGAscript T7 transcription kit (Thermo Fisher Scientific) was used to produce each ESKAPE tmRNAGFP11 before its purification using the corresponding MEGAclear kit ...
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bioRxiv - Microbiology 2020Quote: ... and the MEGAscript® T7 Kit (ThermoFisher Scientific) was used for in vitro transcription of the fragment ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.05 mg/mL T7 RNA polymerase (Thermo Fisher), and in a 50 μL reaction volume ...
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bioRxiv - Biochemistry 2020Quote: ... 10 U/μL T7 RNA polymerase (ThermoFisher Scientific), 1 U/μL RiboLock RNase Inhibitor (ThermoFisher Scientific) ...
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bioRxiv - Genetics 2020Quote: ... The MEGA shortscript T7 kit from Life Technologies was used to synthesize the sgRNA from the T7 promoter ...
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bioRxiv - Microbiology 2020Quote: ... The mMESSAGE mMACHINE T7 ultra transcription kit (Invitrogen), which includes a cap analogue Anti-Reverse Cap Analogue (ARCA) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... using T7 or SP6 RNA Polymerases (ThermoFisher Scientific) along with DIG-UTP-labelling mix (Millipore Sigma) ...
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bioRxiv - Biochemistry 2019Quote: ... 10 U/µl T7 RNA polymerase (ThermoFisher Scientific), 1 U/µl RiboLock RNase Inhibitor (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... SP6 and T7 polymerases were purchased from ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... A MEGAScript T7 in vitro transcription kit (Invitrogen) was used to obtain viral RNA ...
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bioRxiv - Genetics 2020Quote: ... using mMESSAGEmMACHINE™ T7 Transcription Kit (Ambion, AM1344) and Poly(A ...
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bioRxiv - Immunology 2021Quote: ... and transcribed using MEGAscript T7 Transcription Kit (Invitrogen) followed by MEGAclear Transcription Clean-Up Kit (Invitrogen) ...
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bioRxiv - Physiology 2022Quote: ... and MEGAshortscript T7 Kit (ThermoFisher Scientific, AM1354, USA) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... and either SP6 or T7 RNA polymerase (Invitrogen). Primers used for generating probes to amplify the 3’-untranslated regions of type IV collagen transcripts (α1-α6 ...
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bioRxiv - Cell Biology 2023Quote: ... IVT was performed with MegaShortScript T7 Kit (Invitrogen) for the 359 and 67 nucleotide long fragments and subsequent RNA purification was performed according to manufacturer protocol ...
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bioRxiv - Microbiology 2023Quote: ... inserted into bacterial T7 expression vector pRSETa (Invitrogen), and expressed ...
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bioRxiv - Developmental Biology 2023Quote: ... dsRNA was generated using T7 Megascript kit (Invitrogen). dsRNA was injected at a concentration of 2 μg/µL in nuclease-free water ...
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bioRxiv - Immunology 2022Quote: ... prepared by mMessage mMachine T7 Ultra transcription (ThermoFisher) or morpholinos were injected into one to two-cell stage embyos from Tg(lysC:CFP-NTR)vi002x Tg(BACmmp9:Citrine-CAAX)vi003 crosses.
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bioRxiv - Molecular Biology 2023Quote: ... using the mMESSAGE mMACHINE T7 Kit (Ambion, USA) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... using the MEGAScript T7 transcription kit (Invitrogen, AMB13345). The amplified RNA (aRNA ...
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bioRxiv - Developmental Biology 2023Quote: ... sgRNA was then transcribed (MEGAshortscript T7, Invitrogen AM1354), cleaned (RNA Clean and Concentrator ...
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bioRxiv - Evolutionary Biology 2024Quote: ... T7 or Sp6 in vitro transcriptional kit (Invitrogen). Oocytes from Xenopus laevis frogs were harvested as previously described (Sheng et al. ...
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bioRxiv - Microbiology 2020Quote: ... media with 0.05 mg/mL bovine pituitary extract and 5 ng/mL human recombinant epidermal growth factor (Gibco - Thermo Fisher Scientific, MA, USA). All culture conditions contained a 5% antibiotic / antimycotic mixed solution (Nacalai Tesque) ...
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bioRxiv - Immunology 2020Quote: ... Cells were cultured for 1 week at a density of 106/well in 6 well plates with 50 ng/ml recombinant human macrophage colony stimulating factor (M-CSF) (Life Technologies, Carlsbad, CA), 1x GlutaMAX-I (Life Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells stably expressing human recombinant LOXL1 were maintained in culture (37°C, 5% CO2) at log-phase growth in advanced DMEM (Thermo Fisher Scientific, 12491015) with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: ... cells at the end of 1-day culture were pulsed for 20 minutes with 40 ng/ml of recombinant human IFN-γ (Gibco/Thermo Fisher), 100 IU/ml of recombinant human IL-2 (Roche Diagnostics ...
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bioRxiv - Microbiology 2022Quote: ... and Omicron RBD-his protein were coated overnight at 4 °C in a 96-well plate (MaxiSorp Nunc-immuno, Thermo Scientific, USA). Wells were blocked with 5% non-fat milk (Biofroxx ...
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bioRxiv - Microbiology 2022Quote: ... and Omicron RBD-his protein were coated overnight at 4 °C in a 96-well plate (MaxiSorp Nunc-immuno, Thermo Scientific, USA). Wells were blocked with 5% non-fat milk (Biofroxx ...
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Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: The fluorescence-labeled AFP1-His proteins were prepared according to the manufacturer’s instruction of the DyLight 550 Antibody Labeling kit (Thermo Fisher; Cat#84530). One OD600 of cells washed with MES buffer was subjected to immunostaining using the primary anti-HA antibody and AF488-conjugated secondary antibody or wheat germ agglutinin conjugated antibody (WGA-A488 ...
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bioRxiv - Immunology 2022Quote: ... Soluble forms of the SOSV antigens with were purified by using the proteins’ 6×-His tag or StrepII tags and Expi293F cells and Expifectamine 293 expression system (Thermo Fisher Scientific) as discussed below at day 5 to 7 post-transfection ...
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bioRxiv - Biochemistry 2023Quote: ... of randomized RNA library produced for IVM-seq experiment was incubated with equal molarity of 6x His-tag fused target protein complex immobilized on 7 μL Ni-NTA magnetic agarose beads (Thermo Scientific 78605) in the SELEX binding buffer (10 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2024Quote: ... The precipitated proteins were visualized by SDS-PAGE and Western blot analysis using an anti-His tag monoclonal rabbit antibody (Thermo Fisher Scientific) to determine the protein distribution in each fraction.
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bioRxiv - Microbiology 2020Quote: Recombinant expressions of proteins involved in this study were performed by using the ExpiCHO™ expression system (Thermo Scientific, A29133). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies produced from cell culture supernatants were purified immediately by affinity chromatography using recombinant Protein G-Agarose (Thermo Fisher Scientific) or CaptureSelect™ IgA Affinity Matrix (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Antibodies produced from cell culture supernatants were purified immediately by affinity chromatography using recombinant Protein G-Agarose (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Recombinant E protein was produced by transient transfection of Expi293F cells using a ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Cell supernatants were harvested 4 days after transfection and then concentrated before exchange into 2× PBS at pH 6.5 and finally into 2× PBS at pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: Ribonucleoprotein complexes containing 3.9 picomoles of ATF3 sgRNA guides and 3 picomoles of purified recombinant Cas9 protein (Synthego) were transfected into HEK293T cells using Lipofectamine CRISPRmax (Invitrogen). 72 hours post transfection ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were sonicated and recombinant proteins purified in a single step on Ni-agarose column according to manufacturer instructions (Invitrogen). Protein samples were dialyzed against PBS with 5 mM MgCl2 and 0.1% glycerol for 2h and used immediately in experiments ...
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bioRxiv - Microbiology 2019Quote: ... Stable cell lines expressing the recombinant protein were initially maintained in complete medium comprising of serum free medium Sf-900 II SFM (ThermoFisher) complemented with 10% v/v foetal bovine serum (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... recombinant spike protein and ACE2 was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Microbiology 2020Quote: A baculovirus expression system was used for the expression of DUB2 recombinant protein in sf9 insect cells as described in the Bac-to-Bac Baculovirus Expression protocol (Invitrogen). The gene was first cloned into the PFastBacNKI-his-3C-LIC vector using Ligation Independent Cloning (LIC) ...
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bioRxiv - Microbiology 2019Quote: ... bacteria resuspended to OD660 0.3 were incubated with 1 µg of recombinant protein in the presence of 1x protease inhibitors (Pierce, Thermo Scientific) in sterile 1.5 ml microcentrifuge tubes with gentle end-over-end mixing for 30 min at 25°C.
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bioRxiv - Immunology 2021Quote: For competitive ELISA recombinant hACE2 protein were immobilized (400 ng per well) on immunoplates (Nunc MaxiSorp, Thermo Fisher Scientific, USA). RBD-specific nanobodies were mixed with HRP-conjugated recombinant RBD protein (0.2 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... LPS-primed THP-1 cell lysates or purified recombinant NLRP3 protein were pre-cleaned using streptavidin magnetic beads (88816, ThermoFisher) to remove nonspecific binding ...