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Citations for Thermo Fisher :
1201 - 1250 of 10000+ citations for 6 Chloro 9 fluorobenz 9 isoquinoline 5 10 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... C57BL/6 P4-5 mice pups were rapidly decapitated and brains were processed in Hibernate-A medium (Thermo Fisher Scientific, A12475-01). Resulting tissue was enzymatically disassociated in buffer containing HEPES-HBSS with DNase (Worthington Biochemical LS002007 ...
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bioRxiv - Neuroscience 2022Quote: ... and 12 old (21 months) male C57BL/6 animals (combined over 2 independent experiments) were intraperitoneally injected with 5-ethynyl-2’- deoxyuridine (EdU) (Fisher Scientific, A10044) (resuspended in PBS at 5 mg/mL ...
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bioRxiv - Immunology 2022Quote: ... Media was removed from THP-1 monocytes cultures (5-6 x 105 cells/ml) and cells were washed once with DPBS (Thermo Fisher Scientifics). A total of 2 x 106 cells were resuspended in 50 μl of BTXpress solution and the Cas9-sgRNA complex was added ...
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bioRxiv - Developmental Biology 2022Quote: ... NC cells were aggregated into 3D spheroids (5 million cells/well) in Ultra Low Attachment 6-well culture plates (Fisher Scientific, 3471) and cultured in Neurobasal (NB ...
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bioRxiv - Microbiology 2023Quote: ... After the secondary antibody was washed three times for 5 min, nuclei were stained with DAPI (4’,6- Diamidino-2-Phenylindole, Dihydrochloride) (#D1306, Thermo Fisher Scientific) (dilution 1:1,000 in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... 2’,7’-Bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM) was purchased from Molecular Probes (Invitrogen, Carlsbad, CA, USA). Fluorescein isothiocyanate (FITC)- and tetramethylrhodamine (TRITC)-conjugated goat anti-mouse and rabbit IgG antibodies were purchased from Jackson ImmunoResearch (West Grove ...
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bioRxiv - Biophysics 2022Quote: ... EVs were stained with 5-(and-6)-Carboxyfluorescein diacetate succinimidyl ester (CFDA-SE, hereinafter referred as CFSE) (Thermo Fisher, Catalog No. C1157) and separated as described previously [10] ...
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bioRxiv - Physiology 2023Quote: ... sections were incubated for 5 min with 4’,6-diamidino-2-phenylindole, dihydrochloride (DAPI, Dojindo, D523) and then mounted with PermaFluor (Thermo Fisher Scientific). Images were acquired using a BC43 or LSM800 instrument equipped with a Zeiss Axio Observer Z1 and a LSM 800 confocal unit with Airyscan module ...
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bioRxiv - Biochemistry 2023Quote: ... 6 μL was injected onto an Acclaim PepMap 100 column packed with 2 cm of 5 μm C18 material (Thermo Fisher, 164564) using 0.1% formic acid in water (solvent A) ...
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bioRxiv - Biophysics 2024Quote: Quantitative determination of intracellular pH (pHi) was performed using the cell-permeant ratiometric pH indicator SNARF™-5F 5-(and-6)-carboxylic acid AM (Thermo Fisher) in live imaged N2a cells at 48 hours of differentiation ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were rinsed with serum-free RPMI-1640 medium before being transfected with 5 µL of siRNA pool (20 µM) and 5 µL Lipofectamine 2000 in a 6-well plate in 1 mL of antibiotic-free Opti-MEM (Gibco, #31985-062) medium plus 2 mL antibiotic-free cell-culture medium ...
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bioRxiv - Biophysics 2021Quote: ... buffer B (5 mM Tris-HCl pH 8.0, 10 mM MgCl2, 1 mM EDTA; Ambion), BSA-biotin (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... 10 µg of DNA was incubated with 5 µl of CellFectin reagent (Thermo Fisher Scientific) and added to sf9 cells grown in Insect-Express medium (Lonza ...
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bioRxiv - Neuroscience 2021Quote: ... 5 μl of tracer cocktail (10 mg/ml EZ-Link NHS-Biotin (Thermo Fisher: 20217) and 10 kDa Dextran Alexa Fluor 488 (Thermo Fisher ...
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bioRxiv - Molecular Biology 2019Quote: ... 100 nM E2 or ethanol and 10 µM of 5-ethynyl-2’-deoxyuridine (EdU, Invitrogen) for 24h ...
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bioRxiv - Bioengineering 2020Quote: ... ITS+1 (10 µg/mL insulin, 5.5 µg/mL transferrin, 5 ng/mL selenium; Gibco), 100 µM ascorbic acid 2-phosphate (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... and at 5 days post infection cells were fixed in 10% formalin solution (Fisher Scientific) for 1 hr ...
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bioRxiv - Bioengineering 2022Quote: ... 4.0 μL cell Tracker Green 5-chloromethylfluorescein diacetate (CMFDA, 10 μM; Thermo Fisher Scientific, Inc.) and 16.0 μL propidium iodide (PI ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Each 10 μL reaction contained 5 μL of PowerSYBR Green PCR Master Mix (Applied Biosystems), forward and reverse primers at a final concentration of 0.2 μM ...
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bioRxiv - Biochemistry 2022Quote: ... 10 μl of 5 μM siRNA was diluted into 250 μl of Opti-MEM (Gibco) and 10 μl of Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... in a humidified atmosphere at 37°C with 5% CO2 supplemented with 10% FBS (Gibco), penicillin (50 U/ml ...
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bioRxiv - Genetics 2022Quote: ... after which 5 ul of RNase A was added (10 mg/ml, Thermo Scientific #EN0531). We followed all other standard manufacturer protocols ...
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bioRxiv - Pathology 2019Quote: ... pH 7.4 plus 10 mM EDTA and 5% molecular grade BSA (ThermoFisher Scientific, Waltham, MA).
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bioRxiv - Microbiology 2020Quote: ... 5-10 ul of each sample was loaded on 4-12% Bis-Tris gels (Thermofisher).
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bioRxiv - Immunology 2020Quote: ... After this 1⨯10 5 BMDC per condition were cultured on a collagen (Fisher Scientific) coated6-well plate (Corning ...
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bioRxiv - Developmental Biology 2022Quote: ... The right ventricle was perfused with 5-10 mL of cold PBS (Gibco, 10010-023) to clear blood from the lungs ...
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bioRxiv - Microbiology 2022Quote: ... and rabbit anti-TLR-5 polyclonal antibody (10 µg/ml, 36-3900, Thermo Fisher Scientific).
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bioRxiv - Immunology 2022Quote: ... denatured at 95 °C for 5 min and loaded on a 10 % acrylamide gel (Invitrogen) for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Microbiology 2023Quote: ... 200 µl clarified lysate were combined with 5 µl RNase I (10 U/µl, Invitrogen). Reactions were incubated at RT for 45 min with shaking ...
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bioRxiv - Neuroscience 2023Quote: ... that was supplemented with heat-inactivated 10% FBS and 5% HS (Gibco, Billings, MT, USA), 2 mM L-glutamine (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... 5-10 ng of cDNA were mixed with Power SYBR Green MasterMix (ThermoFisher, Cat#4367659). Each Ct was measured using the QuantStudio™ 7 Flex Real-Time PCR System ...
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bioRxiv - Neuroscience 2023Quote: ... for 10 min at 37°C/5% CO2 before rat primary cortical astrocytes (Gibco, #N7745100) were plated at a density of 100 cells/mm2 48 hours prior to plating of neurons Subsequently ...
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bioRxiv - Microbiology 2023Quote: ... and 2-5 μl sample were loaded onto 10-20% SDS-tricine gels (Invitrogen, #EC66252BOX) and LPS was silver stained as described previously [47] ...
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Validating Minimally Invasive Laser Doppler Flowmetry for Serial Bone Perfusion Measurements in MicebioRxiv - Bioengineering 2019Quote: Systemic inflammation was examined by quantifying serum concentrations of the proinflammatory marker interleukin 6 (IL-6) with an ELISA (IL-6 Mouse ELISA kit, KMC0061, Invitrogen, Carlsbad, CA). Samples were prepared according to the manufacturer’s instructions and measured using a plate reader (Synergy H1 ...
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bioRxiv - Biochemistry 2021Quote: ... The 5 mL HisTrap column was washed with 10 column volumes of wash buffer (2× GIBCO 14200-075 PBS, 5 mM Imidazole, pH 7.5) followed by 6 column volumes of elution buffer (2× GIBCO 14200-075 PBS ...
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bioRxiv - Microbiology 2021Quote: ACPL-GFP D10 and NF54 PfMev parasites were synchronized with 5% (w/v) D-sorbitol for 10 minutes at room temperature and returned to culture in 10 µM fosmidomycin (Invitrogen Life Technologies F23103), 100 nM atovaquone (Caymen Chemicals 23802) ...
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bioRxiv - Molecular Biology 2022Quote: ... 5’-labeling was performed by incubating 10 pmol oligonucleotide with 1 μL of 32P-γ-ATP (10 μCi/μL) and 5 U T4 Polynucleotide Kinase (Thermo Fisher Scientific) for 1 h at 37°C in a 10 μL reaction ...
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bioRxiv - Immunology 2023Quote: ... and samples were sonicated at 10 kHz for two 10-second intervals with a 5 second break in between intervals (Fisher Scientific UltraSonic Processor). Samples were then heated to 95°C for 5 min ...
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bioRxiv - Molecular Biology 2023Quote: ... was maintained in a humidified incubator at 37°C in 5% CO2 in αMEM supplemented with 10% fetal bovine serum (FBS) and gentamicin sulfate (10 μg/ml) (Life Technologies, Carlsbad, CA).
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bioRxiv - Bioengineering 2021Quote: ... 50 μg of pre-aliquoted Tandem Mass Tag 6-plex (TMT-6, Thermo Scientific) was resuspended in 20 μL anhydrous acetonitrile ...
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bioRxiv - Cancer Biology 2022Quote: ... Plasma IL-6 was measured by immunoassay (ProQuantum Human IL-6 Immunoassay Kit, ThermoFisher) and run on the ABI 7300 (Applied Biosystems ...
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bioRxiv - Bioengineering 2023Quote: FsC[Fe3+] was labelled by 6-carboxyfluorescein (6-FAM) (Thermo Fisher Scientific, Waltham, USA) to indicate the targeting of FsC[Fe3+] to C ...
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bioRxiv - Immunology 2022Quote: ... BMDMs were seeded into 6-well plates (one million per well) in DMEM with 10% dialyzed FBS (Thermo Scientific, Waltham, MA), 25 mM unlabeled glucose ...
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bioRxiv - Pathology 2019Quote: ... IL-6 and IL-10 levels were assessed using ELISA kits (Cat# 88-7324-22, BMS603-2, 88-7105-22 Thermofisher, USA). The intensity of the colour was measured using a microplate reader (Thermal ...
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bioRxiv - Molecular Biology 2019Quote: ... Differentiation experiments were carried out for 10 days on gelatin-coated 6-well plates by transitioning cells in Dulbecco modified Eagle medium (DMEM, ThermoFisher Scientific) with 10% of fetal bovine serum ...
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bioRxiv - Bioengineering 2020Quote: ... and subjected to heat-induced antigen retrieval using sodium citrate buffer(10 mM, pH 6, Fisher Scientific International, Inc., Pittsburgh, MA) at >80°C ...
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bioRxiv - Microbiology 2021Quote: ... cells were infected with MVA or MVAΔE5R at MOI 10 for 6 h in the presence of MG132 (25 μg/ml) and lysed in RIPA lysis buffer (ThermoFisher, 89901) on ice for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... Cas9-expressing hCMEC/D3 cells were each transduced separately by lentiviral particles expressing sgRNAs and selected by puromycin resistance at 10 μg/mL for 6 days (Life technologies). sgRNA sequences are listed in Table 2.
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bioRxiv - Microbiology 2022Quote: ... 5 mg BA disks were prepared by transferring 10 μL of preheated 500 mg/mL BA in Dimethyl sulfoxide (DMSO) stock to blank antimicrobial disks (6 mm, Fisher Scientific). Single disks containing either 5 mg BA or 25 μg FLC (6 mm ...
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bioRxiv - Neuroscience 2020Quote: ... qPCR was performed in a final volume of 10 μL per reaction in 384-well PCR plates using a thermocycler (QuantStudio 6 Flex, Applied Biosystems). Briefly ...