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Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for 6 Chloro 9 fluorobenz 9 isoquinoline 5 10 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... glycopeptides were resuspended for 5 minutes in 10 µL of 400 mM HEPES pH 10 prior to the addition of the TMTpro reagents (Thermo Fisher Scientific). To quantify the full proteome ...
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bioRxiv - Cell Biology 2024Quote: MSCs were cultured as 3D pellets (2.5×10 or 5×10 cells/pellet) in chondrogenic medium (DMEM high-glucose (Gibco, Life Technologies, Germany), 0.1 μM dexamethasone ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were processed into slabs of tissue ∼5-10 mm thick and placed in 10% neutral buffered formalin (Fisher Scientific; 426-796) for 24-36 hours at 4°C prior to transfer to 70% ethanol ...
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bioRxiv - Microbiology 2024Quote: ... and also cultured16 the fluid by spread-plating 20 microliters onto a pre-reduced tryptic soy 5% defibrinated sheep blood agar (80% N–10% H– 10% CO2, at 37°C; Thermo Fisher Scientific) using a variable-atmosphere anaerobic Whitley workstation A85 (540 plate capacity ...
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bioRxiv - Microbiology 2019Quote: N2a cells were transiently transfected at ≈70% confluence in a 6-well plate by adding per well 10 μL Lipofectamine 2000 (Invitrogen) together with 3 μg of the respective pcDNA3.1 plasmid derivative ...
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bioRxiv - Immunology 2021Quote: ... and KP cells (lung adenocarcinoma; C57BL/6 mice) (Xue et al., 2011) were grown in IMDM with 10% heat-inactivated FBS (GIBCO), 100 U/ml penicillin/streptomycin (GIBCO ...
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bioRxiv - Cell Biology 2019Quote: ... Slides were washed 3 × 10 min with PBS and nuclei stained with 1 µg/mL 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen) in PBS for 15 min ...
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bioRxiv - Cell Biology 2019Quote: U2OS cells were seeded on 25 mm coverslips in a 6-well dish and incubated with 10 μM BrdU (Invitrogen) for 12 to 24h ...
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bioRxiv - Cell Biology 2020Quote: ... Seoul National University) were seeded into 6-well culture plates using DMEM supplemented with 10% newborn bovine serum (Thermo Fisher), and 1% penicillin/streptomycin at a density of 1 × 105 cells/well ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were cultured in a 6-well plate with the DMEM medium (Thermo) and 10% FBS (Gibco, cat# 10091148) to generate the lentivirus ...
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bioRxiv - Cell Biology 2021Quote: ... on 12 or 15 mm coverslips in a 6-well plate and 10 nM siRNA-1 or -2 was transfected using RNAi MAX reagents (Invitrogen) according the manufacturer protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... 1µM PRMT5 inhibitor or negative control treated (3 or 6 day) OCI-AML-20 cells were incubated with 10 µM EdU (Invitrogen) for 2 h at 37 °C ...
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bioRxiv - Systems Biology 2022Quote: ... Transcriptomics using the isolated mRNA from liver tissues (0, 2, 4, 6, 8 and 10 weeks; n=3 per time point) was performed by Affymetrix GeneChip®Mouse Gene 2.0 ST Arrays (902118) ...
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bioRxiv - Genetics 2022Quote: Cells plated in 10 cm Petri dishes were lysed and scrapped in 6 mL ice-cold TRIzol™ Reagent (Invitrogen) added directly to the culture dish after removal of the growth medium ...
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bioRxiv - Immunology 2022Quote: ... bone marrow progenitors were collected from the femurs of adult C57BL/6 WT mice and 10 × 106 progenitor cells were cultured in RPMI media (Gibco) supplemented with 10% FBS (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... For glutamine starvation experiments cells were washed once with PBS and incubated for 6 hours in DMEM/F12 without glutamine and supplemented with 10% (v/v) dialyzed FBS (Gibco), 1% (v/v ...
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bioRxiv - Molecular Biology 2020Quote: Targeting was carried out by transfecting 3 μg of each Cas9 guide and 10 μg of targeting construct into ESCs in one well of a 6-well plate using Lipofectamine 3000 (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: Targeting was carried out by transfecting 3 μg of each Cas9 guide and 10 μg of targeting construct into ESCs in one well of a 6-well plate using Lipofectamine 3000 (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... ESCs in one well of a 6-well plate were transfected with 10 μg of expression vector using Lipofectamine 3000 (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Two x 106 cells/well of a 6-well plate were seeded in DMEM containing 10% FCS (Invitrogen, Carlsbad; USA), 1% HEPES (Carl Roth ...
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bioRxiv - Cell Biology 2021Quote: ... They were washed in PBST for 6 × 10 min before being incubated with secondary antibody (1:400 in PBST) (ThermoFisher) for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... 20ng of totalRNA was used for library preparation with ERCC Spike-in control Mix A (Ambion 10-6 final dilution). All steps were performed on a Tecan EVO150 ...
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bioRxiv - Pathology 2022Quote: ... One million myoblasts per genotype and per mouse were pelleted at 300g for 5 minutes in 15 mL centrifuge tubes before pellets were transferred into individual wells of a 6-well plate containing 50 % proliferation medium (20 % foetal bovine serum, Gibco 10500-064; 10 % horse serum, Gibco 26050-088 ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... and the supernatant centrifuged twice at 1000g for 10 min at 4°C (Sorvall RC-6 Plus Centrifuge, Thermo Scientific). The supernatant was centrifuged 8000g for 10 min at 4°C and then aspirated without disturbing the pellet ...
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bioRxiv - Immunology 2023Quote: ... One day prior to the co-culture 70- and 20-4_1BBζ cells were defrosted and cultured in a density of 1 × 10^6 cells/mL in RPMI containing1X GlutaMAX (Gibco), 1X P/S and 10% FCS supplemented with 10 ng/mL IL7 and IL15 overnight ...
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bioRxiv - Cell Biology 2023Quote: ... and seeded onto 0.1% gelatine-coated 6-well plates in DMEM supplemented with 10% foetal calf serum (Gibco, ThermoFischer, #26140079) and 1x Glutamax (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were seeded onto 6-well or 96-well plates for 24 hours and followed by siRNA (10 nM) transfection using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Cells were cultured to approximately 60% confluency on 24-or 6-well plate and transfected with siRNAs at final concentrations of 10-25nM using lipofectamine RNAiMAX (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... (6) Negative photoresist SU-8 2000.5 was mixed with Lissamine rhodamine B ethylenediamine (RhBen; ∼10 μg/ml; Thermo Fisher Scientific) and placed in the dark at room temperature (RT ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from 10 to 20 ovaries from 3-6 day-old flies using TRizol (Thermo Fisher Scientific) following the manufacturer’s instructions.
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bioRxiv - Biochemistry 2024Quote: Hepa1-6 cells (mouse hepatoma; ATCC; CRL-1830) were cultured in high glucose DMEM containing 10% fetal bovine serum (FBS) (Gibco) and maintained at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mL of this cell suspension was plated into 3 wells on a bottom layer of 0.5% select agar in RPMI+10% FBS in 6-well non-tissue culture treated dishes (Thermo Scientific). For soft agar inhibitor experiments ...
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bioRxiv - Biochemistry 2024Quote: ... Reactions were performed at 30°C for 10 min and terminated by addition of 0.4% SDS and 6 × loading buffer (Thermo Scientific). The reaction mixture was loaded onto an 0.8% agarose gel in 1x TAE (40 mM Tris ...
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bioRxiv - Immunology 2024Quote: ... We performed reaction in triplicates with 10 µl total volume using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). We normalized qPCR signal from ChIP DNA by 2% DNA input using the formula % of input = 100*2ᶺ(Ct [input]-Log2(50 ...
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bioRxiv - Bioengineering 2021Quote: ... a 6 well plate containing 6 mL FACS Clean (Thermo Fisher, BD 340345), 6 mL FACS Rinse (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... and labelled with 0.7 μM 6-carboxyfluorescin dictate (6-CFDA; Thermo Fisher Scientific) for 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... high dose IL-6 stimulation received 100 ng/ml IL-6 (Gibco; PHC0066) or 100 pM acetic acid vehicle stimulation for either 3-or 24-hours and immediately collected for analysis ...
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bioRxiv - Microbiology 2019Quote: Spotted protein arrays (5 x 6) were printed onto each well of Greiner high-binding 96-well plates (Cat# 655097, Thermo Fisher Scientific) using a sciFLeXARRAYER S12 (Scienion AG ...
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bioRxiv - Molecular Biology 2021Quote: For DNA plasmids: 5×106 HEK293T cells were grown in 6-well plates and Lipofectamine™ 3000 Transfection Reagent (L3000001, ThermoFisher UK) was used to transfect the cells following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: Exon 2 of A3A and exons 5 and 6 of A3B with either 20 or 80 bp of flanking intronic sequences were synthesized (Thermo Fisher Scientific) and cloned in sense orientation using XhoI and NotI restriction sites of Exontrap vector pET01 (MoBiTec ...
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bioRxiv - Microbiology 2019Quote: ... isolated for each sediment-depth explored along SSK42/5 and 6 were shotgun sequenced individually on an Ion Proton sequencing platform (Thermo Fisher Scientific) using 200 nucleotide read-chemistry ...
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bioRxiv - Biophysics 2019Quote: ... the final dye to protein ratio was typically 5-6 dye molecules per protein molecule as measured with a Nanodrop spectrophotometer (Thermo Fisher Scientific). Cells in suspension culture dishes were labeled with 0.5 nM fluorescent IgE for typically 12-20 hours before imaging.
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bioRxiv - Cancer Biology 2020Quote: ... n = 3) or oligopyridylamides (5 µM ADH-1 or ADH-6, n = 3) using a combination of both TriZol (Thermo Fisher Scientific) and RNAeasy Mini Kit (Qiagen ...
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bioRxiv - Biochemistry 2021Quote: ... The mixture was left to equilibrate for 5 min at RT before 6 μL aliquots were placed in crystal-clear vials (Fisher Scientific UK) and snap-frozen in liquid nitrogen ...
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bioRxiv - Microbiology 2021Quote: ... was introduced into 5 × 106 C6/36 mosquito cells per well of a 6-well plate via Lipofectamine 2000 reagent (Thermo Fisher Scientific), following the product’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: Mice were anesthetized using isoflurane and injected with 1% Biocytin-5(and-6-)- Tetramethylrhodamine (Biocytin-TMR: ThermoFisher Scientific; Waltham, MA, Cat# T12921). After 30 minutes in circulation ...
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bioRxiv - Neuroscience 2021Quote: ... The specificity of this antibody has been previously validated by us and others by Western Blot, IF and IHC analyses (5, 6).The Anti-NeuN mouse monoclonal antibody (ThermoFisher MA5-33103) was used for IF ...
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bioRxiv - Immunology 2020Quote: ... or aged (26-28 mo) female C57BL/6 mice and cultured for 7 days at 37°C and 5% CO2 in DMEM (Gibco, 11995-073) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2021Quote: ... 5 x 105 HCT116 cells were seeded in a 6 well plate with Dulbecco’s modified Eagle medium (DMEM, Gibco®, Life Technologies) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... The syn-gRNA oligos and syn-gRNA-NTC were transfected using Lipofectamine RNAiMAX transfection reagent (5 µl per 6-well; Thermo Fisher Scientific) following the manufacturer’s instructions ...