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Citations for Thermo Fisher :
1001 - 1050 of 10000+ citations for 6 Chloro 9 fluorobenz 9 isoquinoline 5 10 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: 100,000 BHK cells were plated into a 6-well plate and transfected with 5 nM of siRNA pool (siTOOL) against Uhrf1 or Usp7 by Lipofectamine 3000 (Invitrogen). After 24 or 48 hours ...
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bioRxiv - Neuroscience 2019Quote: ... post-axotomy cultures were loaded with lipophilic dye N-(3-trimethylammoniumpropyl) -4-(6-(4-(diethylamino) phenyl) hexatrienyl)pyridinium dibromide (FM 5–95; Invitrogen) using KCl mediated depolarization as described previously (Taylor et al ...
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bioRxiv - Developmental Biology 2019Quote: ... E8 medium was replaced daily and hiPSC were gently passaged every 5-6 days by mechanical selection or bulk passaged using non-enzymatic reagents (i.e., Versene solution (ThermoFisher Scientific) or Phosphate-Buffer-Saline (PBS)-based enzyme-free cell dissociation buffer (ThermoFisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... Samples were diluted 1:5 in TE buffer and 6% were mixed with TaqMan Universal PCR Master Mix (Applied Biosystems). The sample mix and TaqMan assays were loaded separately into the wells of 96.96 Gene expression Dynamic Arrays (Fluidigm ...
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bioRxiv - Bioengineering 2021Quote: ... Droplets of 5 μL cell-Matrigel mixture were manually deposited onto a high performance #1.5 glass bottom 6-well plate (Fisher Scientific). Data acquisition was performed using a confocal microscope (Nikon A1R HD25 ...
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bioRxiv - Bioengineering 2020Quote: ... Primary fibroblast passages 2–6 were achieved in culture in 37°C in a 5% CO2 incubator with DMEM High Glucose (Gibco) with 10% FBS to 80% confluence in 100 mm Petri dishes ...
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bioRxiv - Neuroscience 2021Quote: ... samples were washed 5-6 times over 2 hours in PBT at room temperature and incubated with secondary antibodies (Invitrogen) diluted in NGS-PBT for 24 hours at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Transfection was carried out in unsupplemented NBA using 500 ng of the luciferase reporter construct and 20 ng of a normalizer plasmid pGL4.83-mPGK-hRLuc at 5-6 DIV using Lipofectamine 2000 (Thermo Scientific) with DNA to Lipofectamine ratio of 1:2 ...
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bioRxiv - Cell Biology 2021Quote: ... 2X SSC) for 5 min and counter stained with 1 μg/ml DAPI (4’,6-diamidino-2-phenylindole; Life Technologies). Coverslips were mounted in imaging buffer (3.7 μg/μl glucose oxidase and 1U catalase in equilibration buffer ...
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bioRxiv - Cancer Biology 2020Quote: Gene-specific knockdown was achieved by reverse-transfection of PCa cell suspensions (total 5×105 cells) with 12.5 nM siRNA in 6 well plates using RNAiMAX transfection reagent (Life Technologies; Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... 1 µg of plasmid was used to transfect 400,000 cells in each well of a 6-well plate using Lipofectamine 2000 (5 µL; Thermo Fisher) in OptiMEM (400 µL ...
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bioRxiv - Plant Biology 2022Quote: ... and the amplified DNA fragment and an annealed linker oligo (for GFP: primers 5 and 6) were introduced into the pENTR/D-TOPO vector (Invitrogen; sequences were confirmed by Sanger sequencing using primers 7 and 8 ...
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bioRxiv - Cell Biology 2022Quote: ... dissociated neurons were resuspended and cultured at 37°C in a humidified incubator with 5% CO2 and 95% O2 in poly-D-lysine coated 6-well plates in neurobasal media (12349015, Gibco) supplemented with 1% GlutaMAX™ Supplement (35050-061 ...
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bioRxiv - Molecular Biology 2020Quote: ... Trypsinized hippocampi were washed two times with the same HBSS buffer before being triturated 5-6 times with a fire-polished glass Pasteur pipette in BME (Invitrogen) supplemented with 10 percent FBS (VWR) ...
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bioRxiv - Biochemistry 2021Quote: ... the cells were seeded on a 6-well plate with 2 ml of DMEM medium supplemented with 5% horse serum (Gibco). At 24 hours after transfection ...
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bioRxiv - Cancer Biology 2019Quote: The human myeloma cell line KJON (19) was cultured in RPMI with 5 % heat inactivated human serum (HS) and 2 ng/mL interleukin (IL)-6 (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2019Quote: ... PCR was conducted using a 5’ fluorescently-labelled primer (6-FAM, PET, NED, or VIC) (Applied Biosystems, Foster City, CA) and an unlabeled reverse primer ...
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bioRxiv - Neuroscience 2021Quote: ... Longitudinal imaging of vascular leakage in C57BL/6 mouse was performed for 5 days after injecting 600 μg of 3kDa TRITC-dextran (LIF-D-3308, Invitrogen) and 600 μg of 2MDa FITC-dextran (LIF-D-7137 ...
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bioRxiv - Genomics 2020Quote: ... cells were harvested and grown for 5-6 passages in NPC expansion medium (selection medium with the addition of 2% B27 [GIBCO] ...
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bioRxiv - Developmental Biology 2020Quote: Neural retinae were dissected from adult zebrafish dark adapted for 12 hours between 5:30 PM and 6:30 PM and stored in RNAlater (Ambion) at 4°C until extraction ...
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bioRxiv - Microbiology 2019Quote: ... pelleted again by centrifugation and resuspended at 3−5 × 106 cells/ml in sterile PBS containing 1.25 µg/ml 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI; ThermoFisher Scientific) and stored on ice until required ...
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bioRxiv - Cancer Biology 2021Quote: ... 5-FU-D131-#6) were labeled by 15 min incubation with 1 μM CFSE (CellTrace CFSE, ThermoFisher Scientific, Ref: C34554). Cells were then resuspended in PBS supplemented with 30% Percoll ...
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bioRxiv - Cell Biology 2020Quote: ... The production of ROS was assessed by the oxidation of 5-6-chloromethyl-2,7-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) (Invitrogen Corporation). Cells cultured in 96-well plates were incubated with JC1 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we stained the formaldehyde preserves with DAPI (4’,6-diamidino-2-phenylindole, final concentration 5 ug/mL, Thermo Scientific, USA), and counted the microbial cells using hemocytometers (DHC-N01 ...
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bioRxiv - Microbiology 2022Quote: ... for 30 minutes at RT followed by a 5-minute incubation with 300 nM 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen) diluted in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Ss06901209_m1), fibulin-5 (FBLN5, Ss04805024_m11) and samples were run on a QuantStudio 6 Real-Time PCR system (Applied Biosystems #4485694) in technical and biological replicates (n = 3-5) ...
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bioRxiv - Biophysics 2022Quote: Fibroblasts grown in vitro to the third passage were plated in 6-well tissue culture plates (5 × 105 cells per well) in DMEM (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum ...
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bioRxiv - Cancer Biology 2023Quote: Rhodamine tubulin was generated by labeling tubulin with X-rhodamine SE (5(and-6) carboxy-X-rhodamine succinimidyl ester) dye (ThermoFisher). Labeling stoichiometry was determined using a molar extinction coefficient of 115,000 M-1 cm-1 for tubulin and 78,000 M-1 cm-1 for X-rhodamine [58] ...
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bioRxiv - Cell Biology 2023Quote: ... or 5’-TCAAGGTCCCTGATAATTATGGCGA-3’) or scrambled siRNA (non-targeting control) using 6 μL Lipofectamine™ RNAiMAX (Invitrogen™ - Cat.# 13778075). After 24 h ...
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bioRxiv - Developmental Biology 2023Quote: ... Pax9 was cloned from 24 hpf cDNA using the primers pax9F 5’-TCTAGAATGGAGCCAGCCTTT-3’ and pax9R 5’-ATGGATCCTCATAGAGCTGAAGCCACCAG-3’ (Supplementary Table 6) and cloned by TOPO-TA to the pCRII vector (Invitrogen) to create pCRII pax9 ...
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bioRxiv - Genomics 2023Quote: The PPMI iPSC lines were thawed and grown on matrigel (Corning)-coated plates with Essential 8 Flex (E8, Batches 1, 2 and 3) or Essential 6 (E6, Batches 4 and 5) media (both Gibco) for about one month (5 passages) ...
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bioRxiv - Immunology 2023Quote: Zebrafish larvae (6 day-post-fertilization) were incubated in embryo medium with 1% DMSO and 5 mM EdU (Invitrogen C10340) for 8 hours at 29°C after the 16 hour Vc infection ...
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bioRxiv - Biochemistry 2023Quote: ... 3 x 5 min) on a rocking platform and stained with 4′,6-diamidino-2- phenylindole dihydrochloride (DAPI) (Life Technologies) staining (2 μM ...
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bioRxiv - Cell Biology 2023Quote: ... which were blotted with a force of 6 for 5 seconds and immediately vitrified by plunge-freezing in liquid ethane using a Vitrobot Mark IV (ThermoFisher) operating at 4 °C and 100% humidity ...
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bioRxiv - Cell Biology 2024Quote: ... 1 µg of plasmid was used to transfect 750,000 cells in each well of a 6-well plate using Lipofectamine 2000 (5 µL; Thermo Fisher) in OptiMEM (400 µL ...
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bioRxiv - Cell Biology 2023Quote: ... polysome buffer (10 mM Tris, 10 mM MgCl2, 100 mM KCl, 6 mM β-mercaptoethanol, 100 μg/ml cycloheximide, protease inhibitors (Thermo Scientific™, Cat. no. A32961) pH 7.5 ...
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bioRxiv - Cancer Biology 2022Quote: We purchased 6-mercaptopurine monohydrate (6-MP, #AC226520050) from Thermo Fisher.
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bioRxiv - Physiology 2023Quote: ... with or without 0.1uM 6-Hydroxyhexanoic acid (6-HHA, ThermoFisher, B24857.03).
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bioRxiv - Cell Biology 2019Quote: Cultured mCSCs and mBMSCs were suspended at 5 × 10^5 cells/mL in PBS and 200uL was loaded into EZ Cytofunnels (ThermoFisher, Carlsbad, CA) within a Shandon Cytospin 4 (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: ... organoids (after 1 week of culture) were incubated (37°C, 5% CO2, 1 h) with 10 µM EdU (5-ethynyl-2’-deoxyuridine, Thermo Fisher Scientific), a nucleoside analog of thymidine incorporated into DNA during active DNA synthesis ...
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bioRxiv - Microbiology 2022Quote: ... counted with a haemocytometer to a concentration of 5 × 10^5 cells/mL and seeded into 96-well microplate (Nunc, Thermo Scientific).
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bioRxiv - Developmental Biology 2022Quote: ... All other lines were passaged at approximately 80% confluency (every 4-5 days on average) as tiny clusters (3-5 cells on average) using 0.5 mM EDTA (15575020, Gibco, concentration 10 mM).
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bioRxiv - Developmental Biology 2023Quote: ... Extended culture up to day 10 was obtained upon extracellular matrix supplementation in IVC2 media starting from 5 dpa with 5% Geltrex (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed four more times in 0.1 M PBS with 0.5% Triton X-100 for 5-10 minutes and then mounted on glass slides using Fluoromount-G with DAPI (Thermo Fisher Scientific). Individual sections were imaged using a slide scanner (Olympus ...
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bioRxiv - Plant Biology 2024Quote: ... 5 mM EDTA, 5 mM DTT, 10 mM β-mercaptoethanol, 1% SDS, 1 mM PMSF, and 1X protease inhibitors from Thermo Fisher), and the plant debris was removed by centrifugation ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were seeded out at 5 million cells per 10 cm dish in medium containing 10% FBS/high glucose DMEM (Life Technologies). Medium was changed 2 hours post seeding and cells were subsequently maintained in Neurobasal medium supplemented with Glutamax and B27 supplement (Life Technologies).
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bioRxiv - Microbiology 2021Quote: ... home-made lysogeny broth (10 g/L Tryptone, AppliChem; 10 g/L NaCl, Fisher scientific; 5 g/L Yeast Extract, AppliChem) was used or ...
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bioRxiv - Neuroscience 2020Quote: ... 5 μL the corresponding amine-based tandem mass tag (TMT) 10-plex reagents (10 μg/μL) (Thermo Fisher Scientific, Cat # 90406) was added and incubated for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... and cells were washed three times with quenching buffer (10 mM sodium azide, 10 mM sodium ascorbate and 5 mM Trolox in Dulbecco’s PBS (Life Technologies 14190169). Cells were rinsed off the dishes with DPBS ...
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bioRxiv - Cell Biology 2024Quote: MSCs were cultured as 3D pellets (2.5×10 or 5×10 cells/pellet) in chondrogenic medium (DMEM high-glucose (Gibco, Life Technologies, Germany), 0.1 μM dexamethasone ...