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Citations for Thermo Fisher :
1451 - 1500 of 10000+ citations for 6 Chloro 9 fluorobenz 9 isoquinoline 5 10 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... in a QuantStudio 6 Flex (ThermoFisher). ΔCT for each primer pair was determined by subtracting the individual CT value from the CT value of seryl-tRNA synthetase (PF3D7_0717700 ...
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bioRxiv - Immunology 2022Quote: BMDMs obtained by standard procedure were seeded into 6-well plates (1X106 cells per well) in DMEM with 10% dialyzed FBS (Thermo Scientific, Waltham, MA), 4 mM glutamine ...
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bioRxiv - Cell Biology 2021Quote: Following a 90 min feed with 5% chicken egg yolk, guts were dissected from larvae (6 dpf, 10 guts pooled per sample) and stored in RNAlater (Thermo Fisher Scientific AM7020) at 4°C for 1 week ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibody amplification was done in 6-mL 1:600 (10 μl) Alexa Fluor 568 donkey anti-rabbit IgG (Thermo Fisher, Lot# 2044343) for 6 days.
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bioRxiv - Plant Biology 2021Quote: ... The liquid syringe was washed 6 times with each of 10 µL of 1:1 (v/v) acetone:hexane and HPLC grade methanol (Fisher Scientific Co, Edmonton Alberta) both before and after the liquid injection.
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bioRxiv - Microbiology 2022Quote: ... The cells were again washed and incubated for 10 min in a solution of 4’,6’-diamidino-2-phenylindole (DAPI) (Invitrogen, Thermo Fisher Scientific). Cells were imaged using a Leica DM IRB epifluorescence microscope ...
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bioRxiv - Physiology 2019Quote: ... containing thirty pre-exposed or control 10 dpf larvae and three magnetic stir bars (25 × 6 mm, Fisherbrand, #10226853, Fisher scientific, Leicestershire, UK) distributed equidistantly along the bottom of the cage were placed on top of the magnetic stirrer plate (Variomag ...
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bioRxiv - Cell Biology 2021Quote: ... or Nunc 6-well plates (2.5×10 cells/well) were or 2,5 μ jetPRIME® Polyplus transfection reagent or Lipofectamine 2000® (Thermo Fisher Scientific); according to the manufacturers’ instructions ...
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bioRxiv - Neuroscience 2022Quote: ... slices were rinsed 6×10 minutes and incubated with an AlexaFluor488-conjugated goat-anti-guinea pig IgG (1:200, Invitrogen A-11073, RRID:AB_2534117) for 4 hours ...
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bioRxiv - Molecular Biology 2023Quote: HEK293T cells (ATCC) were cultured in 6-well tissue culture plates (CytoOne, USA Scientific) using Dulbecco’s Modified Eagle Medium (Gibco, supplemented with 10% FBS) at 37°C ...
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bioRxiv - Immunology 2020Quote: ... were stained with 0.25 ug of Mamu MR1 5-OP-RU or Ac-6-FP tetramer for one hour in the presence of 500 nM Dasatinib (Thermo Fisher Scientific; Cat No. NC0897653). When TCRVα7.2 co-staining was performed ...
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bioRxiv - Cancer Biology 2020Quote: Gene-specific knockdown was achieved by reverse-transfection of PCa cell suspensions (total 5×105 cells) with 12.5 nM siRNA in 6 well plates using RNAiMAX transfection reagent (Life Technologies; Thermo Fisher Scientific, Scornsby, VIC, AUS), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... THP-1 cells (1.5×105 cells/mL) were labeled with a fluorescent dye 2’,7’-bis(carboxyethyl)-5 (6)-carboxyfluorescein-AM (Thermo Fisher Scientific B1150; 1 mg/mL) in serum-free RPMI medium (Thermo Fisher Scientific 11875093 ...
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bioRxiv - Cancer Biology 2019Quote: ... was cultured in RPMI with 5 % heat inactivated human serum (HS) and 2 ng/mL interleukin (IL)-6 (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). In vitro experiments with KJON cells were performed with 2 % HS in RPMI and IL-6 (1 ng/mL) ...
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bioRxiv - Microbiology 2021Quote: ... were stained with 0.25 ug of Mamu MR1 5-OP-RU or Ac-6-FP tetramer for one hour in the presence of 500 nM Dasatinib (Thermo Fisher Scientific; Cat No. NC0897653). When TCRVα7.2 co-staining was performed ...
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bioRxiv - Neuroscience 2021Quote: ... while levels of IL-6 in supernatants were determined using IL-6 mouse ELISA kit (Thermo Scientific). Similarly ...
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bioRxiv - Microbiology 2022Quote: ... transferred to 6 ml liquid MSgg medium of each well of a 6-well microplate (Thermo Scientific), and then grown for additional four days ...
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bioRxiv - Microbiology 2020Quote: ... 6 × 105 293LTV cells were seeded in a 6-well plate and transfected using Lipofectamine 2000 (Invitrogen) which was complexed with DNA plasmids driving the expression of either VSV G protein (positive control) ...
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Incidence of an intracellular multiplication niche amongst Acinetobacter baumannii clinical isolatesbioRxiv - Microbiology 2021Quote: ... The concentration of IL-6 was quantified by ELISA (Human IL-6 ELISA Ready-SET-Go!, Thermofisher) by following the supplier’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... Coding regions were transferred into Pvha-6-GFP or Pvha-6-RFP vectors by LR reaction (Invitrogen). Constructs were bombarded into unc-119(ed3 ...
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bioRxiv - Biophysics 2023Quote: 6-well plate cell proliferation – Cells were seeded into 6-well plates (Fisher Scientific, 07-200-83) 150,000 cells/well and left at ambient temperature for 20 minutes to ensure homogeneous settling ...
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bioRxiv - Molecular Biology 2021Quote: ... 5pmol of primer (5µl of 5 µM stocks) were end-labeled with 10 units of USB Optikinase (Affymetrix), and 7 µl of 6,000Cu/mmol ATP-ɣ32P (Perkin-Elmer ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed 3 x 5 min and incubated for 10 min with DAPI (1/2000, Invitrogen #D1306) diluted in PBT + 0.5% BSA ...
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bioRxiv - Microbiology 2022Quote: ... 293T/ACE2 cells were cultured at 37 °C with 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM, WAKO) containing 10% fetal bovine serum (Gibco) and penicillin/streptomycin (100 U/ml ...
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bioRxiv - Evolutionary Biology 2020Quote: ... + 5% BCS + 0.4 ug/ml hydrocortisone + 10 ng/ml epidermal growth factor (EGF) + 1% Penicillin-Streptomycin (ThermoFisher Scientific). After two days incubation ...
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bioRxiv - Microbiology 2021Quote: ... qPCR reactions of 10 µL were set up with 5 µL PowerUpTM SYBRTM Green Master Mix (Applied Biosystems), 500 nM of each primer OT5/OT and OT7/OT8 and 20 ng of DNA ...
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bioRxiv - Microbiology 2021Quote: ... 10 ng of cDNA was used as template in the QuantStudio 5 Real-Time PCR system (Applied Biosystems). Amplifications were performed with 5 μl of SYBR® green JumpStart Taq ReadyMix (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were routinely cultured at 37°C with 5% CO2 in complete Dulbecco’s Modified Eagle’s Medium (DMEM} supplemented with 10% FBS (Gibco), 100 U/mL penicillin and 100 μg/mL streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: Cells grown until A600 of ∼0.1 were labelled with 10 μM EdU (5-Ethynyl-2’-deoxyuridine, Thermofisher, C10337) for 15 min after which cells were washed ...
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bioRxiv - Biophysics 2021Quote: ... Cells were incubated with 10 μM of the synthetic nucleoside 5-Ethynyl-2’-deoxyuridine (EdU) (Thermo Fisher Scientific) for 25 min at 37°C and 5% CO2.
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bioRxiv - Cell Biology 2019Quote: ... for 10 minutes, and fixed with methanol:glacial acetic acid, (5:1, v/v) (FisherThermo Fisher Scientific, Rockford, IL). Cells were dropped onto slides ...
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bioRxiv - Developmental Biology 2019Quote: ... Chromatin was pre-cleared with 5 or 10 µl of 1:1 protein A:G Dynabeads (Life Technologies #10015D) and immunoprecipitated with H3K4me1 (Abcam #ab8895) ...
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bioRxiv - Microbiology 2020Quote: ... falciparum were maintained at 5-10% parasitaemia in RPMI1640 supplemented with 0.5% Albumax II (RPMI 1640-Albumax; ThermoFisher) (Trager and Jensen,1976) ...
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bioRxiv - Cancer Biology 2020Quote: Cells were maintained at 37 °C under humidified 5% CO2 in DMEM medium supplemented with 10% FCS (Invitrogen) and 2 mM glutamine ...
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bioRxiv - Cell Biology 2021Quote: HEK293T and SH-SY5Y cell lines were maintained at 37 °C and 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM, Biochrom KG) supplemented with 10% fetal bovine serum (Gibco), 100 U ml−1 penicillin (Gibco) ...
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bioRxiv - Biochemistry 2021Quote: ... Then pH standard buffers (pH ~6.5 and ~7.5 (0.025 M HEPES, 0.105 M KCl, 0.001 M MgCl2)) were prepared with 10 μM nigericin (Invitrogen, N1495) and added to cells to equilibrate pHe and pHi as previously described.28 For each cell ...
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bioRxiv - Neuroscience 2020Quote: ... 5 µl of equal volume supernatants were loaded onto Novex 10% gelatin gels (Life Technologies, Grand Island, NY). Zymography assays were then performed per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Human U-2 OS cells and U-2 OS cells expressing 53BP1-mScarlet from the endogenous promoter25 were grown under standard cell culture conditions (humidified atmosphere, 5% CO2) in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (GIBCO) and penicillin-streptomycin antibiotics ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were treated with 10 μM of Edu (5-ethynyl-2′-deoxyuridine; Invitrogen™, Thermo Fisher Scientific, USA), and incubated for 30 min ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were treated with 10 μM of Edu (5-ethynyl-2′-deoxyuridine; Invitrogen™, Thermo Fisher Scientific, USA), and incubated for 30 min ...
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bioRxiv - Microbiology 2022Quote: ... for 10 minutes and incubated in blocking buffer (2.8 mM KH2PO4, 7.2mM K2HPO4, 5% goat serum (Gibco #16210064), 5% glycerol ...
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bioRxiv - Cell Biology 2022Quote: WT organoids passage 10 were collected 5-7 days after passaging and digested with TrypLE (Thermo Fisher Scientific) for 20 min at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... antibodies were 5-fold serially diluted starting from 100 μg/mL in DMEM medium supplementing 10% FBS (Gibco). Wells without antibodies or containing the same concentrations of REGN10933 were set as control ...
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bioRxiv - Developmental Biology 2022Quote: ... Filtrate was centrifuged and resuspended in stromal culture 5 mL medium containing 10% fetal bovine serum (FBS, Gibco) and 1X ITS-X (ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: ... 10 mM KCl) and then supplemented with 5% (v/v) B-PER Bacterial Protein Extraction Reagent (Thermo Scientific) before sonication ...
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bioRxiv - Molecular Biology 2022Quote: Cells obtained after enzymatic digestion of testis were resuspended in 5-10 ml FACS buffer (1x DPBS [Gibco] supplemented with 1% Pen-Strep ...
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bioRxiv - Microbiology 2023Quote: ... were cultured at 37 °C with 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM, WAKO) containing 10% fetal bovine serum (FBS) (Gibco) and penicillin/streptomycin (100 U/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 μL of Fraction B supernatants and pellets were loaded onto NuPAGE 10% Bis-Tris gels (Life Technologies) and were run at 150 V for 60 minutes ...
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bioRxiv - Microbiology 2022Quote: ... 293T/ACE2 cells were cultured at 37 °C with 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM, WAKO) containing 10% fetal bovine serum (Gibco) and penicillin/streptomycin (100 U/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... organoids were passaged every 1-2 weeks by incubating organoids for 5-10 min in TrypLE Express (Gibco) at 37℃ and mechanical disruption to make organoids fall apart ...