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Citations for Thermo Fisher :
1251 - 1300 of 10000+ citations for 6 Chloro 9 fluorobenz 9 isoquinoline 5 10 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... qPCR was performed in a final volume of 10 μL per reaction in 384-well PCR plates using a thermocycler (QuantStudio 6 Flex, Applied Biosystems). Briefly ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were seeded on a 6-well plate and transfected with 10 pmol of siControl (negative control #4390843, Ambion Silencer Select), siMCM2 (#s8536 ...
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bioRxiv - Microbiology 2021Quote: ... in 6 well multi-dish Nunc UpCell Surface cell culture plates or 10 cm Nunc UpCell Surface cell culture dishes (Thermo Fisher) and differentiated to human monocyte-derived macrophages (hMDMs ...
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bioRxiv - Immunology 2020Quote: ... qPCR was performed in a final volume of 10□μL per reaction in 384-well PCR plates using a thermocycler (QuantStudio 6 Flex, Applied Biosystems). Briefly ...
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bioRxiv - Immunology 2021Quote: ... The isolated nuclei were centrifuged at 500 x g for 5 min at 4°C and respectively resuspended with Nuclei wash buffer stained with 10 µg/ml 4′,6-diamidino-2-phenylindole (DAPI) before FACS (5mg/ml, Invitrogen D1306). After sorting using purity mode ...
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bioRxiv - Genetics 2020Quote: 3×105 HeLa cells were seeded to each well of 6-well plates and cultured in DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Life Technologies) 24 hours before transfection ...
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bioRxiv - Immunology 2021Quote: ... and BMDCs were incubated at a ratio of 10 microspheres per cell for 6 h with 1.0-μm FluoSpheres® carboxylate-modified microspheres (ThermoFisher, F8851) labelled with a red fluorescent dye (580 nm excitation and 605 nm emission) ...
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bioRxiv - Biochemistry 2022Quote: AML12 cells were seeded at 1×105 cells/well in a 6-well plate and transfected with siRNA mixture (f.c. 10 nM) using Lipofectamine 2000 (Invitrogen, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... wash buffers were removed and replaced with a ~7.6 pH nigericin buffer standard (25 mM HEPES, 80 mM KCl, 1 mM MgCl2) supplemented with 10 μM nigericin (Invitrogen, N1495). Cells were incubated at 37°C for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... qPCR was performed in a final volume of 10 μL per reaction in 384-well PCR plates using a thermocycler (QuantStudio 6 Flex, Applied Biosystems). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclear staining of glass-mounted brain sections was performed by 10 min incubation with PBS containing 2.5% DAPI (4’,6-diamidino-2-phenylindole, Thermo Fisher Scientific). Sections were embedded in mounting medium containing 1,4-diazabicyclooctane (DABCO ...
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bioRxiv - Synthetic Biology 2022Quote: ... HEK293T cells were plated in 5mL DMEM supplemented with 10% FBS and 1% PSG in one well of a 6-well plate (Fisher Scientific) for each validation ...
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bioRxiv - Immunology 2023Quote: ... and 1 × 10^6 were processed using Click-iT EdU Pacific Blue Flow Cytometry Assay Kit (Thermo Fisher Scientific Cat. #C10418). The other 1 × 10^6 were subjected to anti-CD3 crosslinking and pERK staining as described below ...
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bioRxiv - Developmental Biology 2023Quote: ... Antigen unmasking was performed by microwaving the slides for 2 × 10 min in citrate buffer solution (pH 6) (Thermo Fisher Scientific). Sections were then blocked for 1 hour at room temperature with 10% normal donkey serum (Chemicon International Inc. ...
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bioRxiv - Cell Biology 2023Quote: ... transfected cells are cultured for 3 days in a 6-well plate before they are gently detached with 10-min incubation in an enzyme-free cell dissociation buffer (Gibco, 13151014). These detached cells are then replated on ECM-coated substrates and imaged after 6 hrs.
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bioRxiv - Molecular Biology 2023Quote: ... and size exclusion chromatography (SEC) using a Superose 6 10/300 GL Increase column (Cytiva, MA) equilibrated in PBS buffer (Thermo Scientific 137mM NaCl ...
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bioRxiv - Cancer Biology 2024Quote: ... The samples were subjected to 6 rounds of 10 sec sonication each at power 3 in a 550 Sonic Dismembrator (Fisher Scientific). Between each round of sonication ...
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bioRxiv - Cell Biology 2023Quote: ... 10 μg total RNA was denatured in formamide loading dye and resolved on the 6% TBE-Urea gel (Novex, ThermoFisher Scientific) in 1 X TBE buffer ...
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bioRxiv - Neuroscience 2024Quote: ... The lentiviral construct of interest was co-transfected with the packaging constructs pCMV-VSV-G and pCMV Gag/Pol at a ratio of 10 µg: 4 µg: 6 µg respectively using Lipofectamine 2000 (60 µL, Invitrogen 11668027). After 4 h ...
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bioRxiv - Cancer Biology 2024Quote: ... the tissue specimens were stained with 4′,6-diamidino-2-phenylindole (DAPI) for 10 min and mounted in VECTASHIELD Vibrance Antifade Mounting Medium (ThermoFisher Scientific). Akoya Biosciences’ PhenoImager HT (formerly known as Vectra Polaris Automated Quantitative Pathology Imaging System ...
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bioRxiv - Cell Biology 2024Quote: ... Transfections were performed with 10 nM of siRNA sequences (6-well plates) by using the Lipofectamine RNAiMax system (Thermo Fisher Scientific). Hypoxia was performed as previously described (24) ...
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bioRxiv - Genetics 2024Quote: ... 25 μg of peptides per condition were labelled with isobaric tags by incubation for 1 hour at RT (TMT 6-plex and 10-plex, Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2024Quote: ... BA disks were prepared by transferring 10 μL of preheated 500 mg/mL BA in dimethyl sulfoxide (DMSO) stock to blank antimicrobial disks (6 mm, Fisher Scientific). MH plates were incubated at 37 °C for 48 h ...
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bioRxiv - Immunology 2024Quote: ... 10 min at 95 °C followed by 40 cycles of 10 sec at 95 °C and 40 sec at 60 °C using the Quant 6 real-time PCR instrument (Applied Biosystems). Samples were considered positive for Giardia spp ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were pelleted down by centrifugation (10628 g at 25°C for 5 min, Thermo Fisher SCIENTIFIC #F9-6 x 1000 LEX rotor). Cells were washed once with water and suspended into 75 mL ice cold water ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were pelleted down by centrifugation (10628 g at 25°C for 5 min, Thermo Fisher SCIENTIFIC #F9-6 x 1000 LEX rotor). The cells were washed once with sterilized water and re-suspended into 6 L MM composed of 1.34% yeast nitrogen base without amino acids (SIGMA Y0626) ...
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bioRxiv - Microbiology 2021Quote: Keratinocytes were seeded at a density of 5×105 cells/well in a 6-well tissue culture plate (Nunc; Thermo Fisher Scientific, Singapore) and grown for 3 days at 37°C in a 5% CO2 humidified atmosphere ...
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bioRxiv - Developmental Biology 2022Quote: Intracellular pH of sea urchin embryos was visualized using pHrodo Red AM intracellular pH indicator or 5-(and-6)-carboxy SNARF-1 (C-1270) (Thermo Fisher Scientific, USA) at final concentrations of 10 and 5 μM ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then rinsed in PBS 6 times for 5 minutes each followed by incubation with goat anti-rabbit IgG Alexa Fluor 594 (#A32740, ThermoFisher Scientific, Waltham, MA) and goat anti-mouse IgG Alexa Fluor 488 (#A11008 ...
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bioRxiv - Developmental Biology 2019Quote: The compound 5-(and-6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA, Image-iT LIVE Green Reactive Oxygen Species Detection Kit, Molecular Probes; Invitrogen, I36007) was used to visualise the in vivo production of ROS ...
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bioRxiv - Microbiology 2021Quote: Keratinocytes were seeded at a density of 5×105 cells/well in a 6-well tissue culture plate (Nunc; Thermo Fisher Scientific, Singapore) and grown for 3 days at 37°C in a 5% CO2 humidified atmosphere ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were washed then incubated in 8 μM of the ROS sensitive probe 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate acetyl ester (CM-H2DCFDA) (Molecular Probes, Eugene Oregon), for 45 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... 95 °C for 5 seconds and 60 °C for 20 seconds in a QuantStudio 6 Flex Real-Time PCR machine (Thermo Fisher Scientific, USA). The detectors used were FAM-BHQ1 and HEX- BHQ1 ...
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bioRxiv - Plant Biology 2022Quote: ... Each sample was incubated with 50 µm of 2’,7’-Bis-(2- carboxyethyl)-5-(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM; Molecular Probes, Eugene, OR) at 28°C ...
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bioRxiv - Immunology 2024Quote: ... followed by 40 cycles of denaturation at 95 °C for 5 sec and extension at 58 °C for 34 sec in the QuantStudio 6 system (Applied Biosystems Co., USA). At the end of PCR ...
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bioRxiv - Cell Biology 2020Quote: ... and optiMEM (1:6; Gibco) or were left untreated ...
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bioRxiv - Microbiology 2021Quote: ... 6% Sodium bicarbonate solution (Gibco), 8% Fetal bovine serum (Sigma Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... TNF and IL-6 (Invitrogen) ELISA kits were used according to the manufacturer’s instructions.
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bioRxiv - Genetics 2019Quote: ... Day 6–7: DMEM (Gibco) with 25 mM glucose containing 1:100 B27 (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... or QuantStudio 6 (Applied Biosystems) thermocycler ...
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bioRxiv - Cell Biology 2021Quote: ... 6′-diamidino-2-phenylindole (Invitrogen). All observations were performed on a Nikon E600 epifluorescence microscope ...
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bioRxiv - Microbiology 2021Quote: ... 6 μg/ml geneticin (Invitrogen) was added ...
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bioRxiv - Neuroscience 2019Quote: ... IL-6 (Invitrogen Carlsbad, CA), TNF-α (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... then 6 µl Lipofectamine (Invitrogen) were added ...
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bioRxiv - Cell Biology 2019Quote: ... 6 mM MgCl2 (Invitrogen, AM9530G), 1 mM each of dNTP’s (ThermoScientific ...
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bioRxiv - Neuroscience 2019Quote: ... hiPSCs were re-plated as clumps in Geltrex (Thermofisher)-coated 6-well plates (Thermofisher) in mTeSR1 (StemCell ...
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bioRxiv - Cancer Biology 2020Quote: ... TRAF-6 (Invitrogen-PA5-29622), and Cyclin D1 (Invitrogen-AHF0082) ...
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bioRxiv - Molecular Biology 2020Quote: ... IL-6 (Invitrogen, California, USA), TNF-α (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... 6 mM L-glutamine (Gibco), 0.1 mM MEM non-essential amino acids (Gibco) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 6 well plates (Biolite, Thermofisher) or 96 well plates (Biolite ...