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Citations for Thermo Fisher :
1151 - 1200 of 2701 citations for 7 METHYL CAMPTOTHECIN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: RNA has been extracted from myotubes differentiated for 7 days using Trizol (Life Technologies) followed by Chloroform/Isoamyl alcool (49:1 v/v) ...
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bioRxiv - Plant Biology 2024Quote: ... was used on the ViiA 7 Real-Time PCR System (Thermo Fisher Scientific, America). Data was analysed with QuantStudio 6 and 7 Pro Real-Time PCR Systems Software (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2024Quote: ... Samples were analyzed on a ViiA 7 Real-Time qPCR System (Thermo Fisher Scientific), and relative expression levels were normalized to a standard reference gene (18s rRNA ...
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bioRxiv - Cell Biology 2023Quote: ... the medium was switched to SFRM (DMEM/F12 [7:3] supplemented with B27 (Invitrogen), 2 mM L-glutamine).
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bioRxiv - Molecular Biology 2024Quote: ... Quantitative PCR was performed on the ViiA 7 Real-Time PCR System (Life Technologies) using the QuantiFast SYBR Green PCR Kit (Qiagen) ...
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bioRxiv - Molecular Biology 2024Quote: ... Quantitative PCR was performed on the ViiA 7 Real-Time PCR System (Life Technologies) using the Luna qPCR Master Mix (NEB) ...
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bioRxiv - Neuroscience 2024Quote: ... qPCR was performed using the QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems). Amplification was performed using a two stages procedure (hold stage at 95 ℃ ...
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bioRxiv - Neuroscience 2024Quote: qPCR was performed on a Quantstudio 7 Flex Real-Time PCR System (Applied Biosystems) using Power SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Biochemistry 2024Quote: ... HEPES pH 7 was added (25 mM final) and semi-soluble IANBD Amide (Invitrogen) from a stock (41 mM ...
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bioRxiv - Bioengineering 2024Quote: COS-7 cells were seeded in a Lab-Tek II chamber slide (Nunc, 154534) and cultured to reach around 50% confluence ...
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bioRxiv - Bioengineering 2024Quote: COS-7 cells were seeded in a Lab-Tek II chamber slide (Nunc, 154534). Cells were firstly extracted in the cytoskeleton extraction buffer (0.2% (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative real-time PCR was performed on a QuantStudio 7 Flex system (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... The qPCR was run on a ViiA 7 Real-Time PCR machine (Applied biosystems) using the following conditions ...
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bioRxiv - Cancer Biology 2023Quote: ... MCF-7 and MDA-MB-231 cells were cultured in DMEM (Invitrogen #D5796-500ML) supplemented with
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bioRxiv - Cell Biology 2019Quote: ... 2,7-Dichlorodihydrofluorescein diacetate (DCFH-DA),3,3’-dihexyloxacarbocyanine iodide [DiOC6(3)] and N-[4-[6-[(acetyloxy)methoxy]-2,7-difluoro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-,(acetyloxy)methyl ester (Fluo-4 AM) were purchased from Molecular Probes (Invitrogen, Eugene, OR, USA). Agarose was obtained from Lonza (Walkersville ...
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bioRxiv - Molecular Biology 2021Quote: Mitochondrial membrane potential of primary neurons was measured by fluorescent staining of Tetramethylrhodamine methyl ester (Image-iT™ TMRM Reagent, ThermoFisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... The mitochondrial membrane potential and dysfunctional mitochondria were measured by staining BM cells with Tetramethylrhodamine Methyl Ester (TMRM 500 nM, Thermo Fisher Scientific, Cat. No. T668), MitoTracker Green (100 nM ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were dialyzed overnight using 7 kDa MWCO membranes (Slide-A-Lyzer G2, Thermo Fisher) into 500 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... The PCR reaction was performed on a ViiA 7 Real-Time PCR System (Applied Biosystems). Samples were run in triplicate and analyzed using comparative CT (2-ΔΔCT ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative RT–qPCR was performed with Real-Time PCR System (Applied Biosystems, Quantstudio 7 Flex), using SYBR Green Master Mix (Applied Biosystems ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... were combined according to the manufacturer’s guidelines and amplified using a QuantStudio 7 Flex (ThermoFisher). The 2-ΔΔCt method was used to calculate transgene expression.
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bioRxiv - Cell Biology 2022Quote: ... Raji cells were then labeled with 10 μM CMAC (7-amino-4-chloromethylcoumarin, Molecular Probes), pulsed with 1 μg/ml SEE (Staphylococcus enterotoxin E ...
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bioRxiv - Immunology 2021Quote: ... Anti-human TIGIT PE Cy 7 (1:50, clone MBSA43, Invitrogen, cat # 25-9500-42), Anti-human CD94 APC (1:100 ...
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bioRxiv - Systems Biology 2019Quote: BT-549 and MCF-7 cells were cultured in full media containing RPMI-1640 (Gibco), 10% heat-inactivated fetal bovine serum (FBS) ...
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bioRxiv - Physiology 2019Quote: Malpighian tubules from 7-day old flies were dissected in Schneider’s medium (Thermo Fisher Scientific) and transfer to poly-L-lysine (Sigma-Aldrich)-covered 35-mm glass-bottomed dishes (MatTek Corporation ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... or 10 mM 2’,7’-dichlorodihydrofluorescein diacetate (DCFH-DA, Thermo Fisher Scientific, Waltham, MA, USA). The cells stained with TMRE or NAO were incubated for 15 minutes at 37◦C whereas cells stained with HE and DCFH-DA were incubated for 30 minutes at 37◦C in the dark ...
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bioRxiv - Immunology 2019Quote: ... Equal protein (20ug per lane) was loaded onto a NuPAGE 7% Tris-acetate gel (Invitrogen). The separated proteins were transferred onto polyvinylidene difluoride membranes ...
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bioRxiv - Microbiology 2019Quote: ... Real-time quantitative PCR analysis was performed (Applied Biosystems ViiA 7 Real-Time PCR System).
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bioRxiv - Physiology 2020Quote: MCF-7 and HeLa cells were transfected with pGL2Basic Neo_NLucPest_3xERE TATA using lipofectamine 2000 (Thermofisher) reagent according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Mammalian COS-7 and MDCK Cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Invitrogen) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Physiology 2020Quote: ... Relative mRNA expression was measured by qPCR (QuantStudio 7 Flex, Applied Biosystems, Foster City, CA) using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) ...
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bioRxiv - Cell Biology 2019Quote: ... The pellet was resuspended 3 times using 7 mL Wheaton Dounce tissue grinder (Fisher Scientific) and centrifuged at 12,000 rpm at 4°C for 30 minutes (Sorvall SS-34 centrifuge rotor) ...
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bioRxiv - Cell Biology 2019Quote: ... 7) rinsed with Milli-Q H2O and mounted with ProLong Diamond (Thermo Fisher Scientific, USA) and cured for 24-48 hours at room temperature.
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bioRxiv - Developmental Biology 2019Quote: ... on a ViiA 7 Real-Time PCR System according to manufacturer’s instructions (Thermo Fisher Scientific). The expression of ef1a was used for normalization ...
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bioRxiv - Plant Biology 2020Quote: Total RNA was isolated from the 7-days-grown Arabidopsis seedlings using TRIzol reagent (Invitrogen), and was treated with RQ1 RNase-free DNase (Promega ...
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bioRxiv - Genetics 2021Quote: ... 7 μg NlaIII digested chromatin was ligated with T4 DNA ligase (EL0013, Invitrogen, 30U/μl) at 20 U/ml in 1X ligation buffer (EL0013 ...
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Epicardial slices: a 3D organotypic model for the study of epicardial activation and differentiationbioRxiv - Bioengineering 2020Quote: ... Real-time PCR was performed on QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems) using QuantiTect SYBR Green PCR Kit (Qiagen ...
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bioRxiv - Bioengineering 2020Quote: HTM cells ± DEX at 7 d were fixed with 4% paraformaldehyde (PFA; Thermo Fisher Scientific) at room temperature for 10 min ...
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bioRxiv - Bioengineering 2020Quote: ... All qPCR reactions were done in a ViiA 7 Real-Time PCR System (Applied Biosystems).
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bioRxiv - Biophysics 2021Quote: ... Proteins were dialyzed overnight using 7 kDa MWCO membranes (Slide-A-Lyzer G2, Thermo Fisher) into physiological buffer (150 mM NaCl ...
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bioRxiv - Physiology 2021Quote: ... mRNA expression was measured on a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems). For each sample ...
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bioRxiv - Microbiology 2020Quote: ... qPCR was performed using a QuantStudio™ 7 flex (Applied Biosystems, Foster City, California, USA) programmed with the following temperature profile ...
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bioRxiv - Microbiology 2021Quote: ... 0.5-1×10^7 parasitized RBCs were incubated with 4 µM BCECF-AM (B1170, ThermoFisher) and 0.02% Pluronic F-127 (p6867 ...
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bioRxiv - Physiology 2020Quote: Relative mRNA expression was measured by qPCR (QuantStudio 7 Flex, Applied Biosystems, Foster City, USA) using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative PCR was performed on a ViiA 7 Real-Time PCR system (Thermo Fisher Scientific) with 1 µl purified DNA ...
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bioRxiv - Biophysics 2021Quote: ... COS-7 and HEK293T cells were cultured in Dulbecco’s modified Eagle medium (DMEM; Gibco, #31053028) supplemented with 10%(v/v ...
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bioRxiv - Biophysics 2020Quote: ... Potassium Chloride (7447-40-7-500G) and glycine (BP381-500) were obtained from Fisher Scientific. Ethanol (200 proof ...
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bioRxiv - Cell Biology 2021Quote: ... qPCR runs were performed on a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems) using SYBR Green reagents (Applied Biosystems ...
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bioRxiv - Biochemistry 2022Quote: ... Reactions were carried out with a QuantStudio 7 Flex Real-Time PCR system (Applied Biosystems) by the RTSF Genomics Core at Michigan State University ...
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bioRxiv - Immunology 2022Quote: ... The cells were incubated in presence of 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA, 25 mM) (Invitrogen) for 30min at 37 °C and 5% of CO2 ...