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Citations for Thermo Fisher :
1201 - 1250 of 2879 citations for 7 METHYL CAMPTOTHECIN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Approximately 7 hippocampi were placed in 5 ml of Trypsin-EDTA (Gibco, 25200-056) and incubated in the water bath at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... qPCR was performed on the QuantStudio 7 Flex Real-Time PCR System (ThermoFisher #4485701) with the following cycling conditions ...
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bioRxiv - Cell Biology 2023Quote: ... flow cytometric apoptosis quantification was performed using the CellEvent Caspase 3/7 kit (ThermoFisher) following the manufacturer’s recommendations.
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bioRxiv - Developmental Biology 2022Quote: TaqMan synthesis for mature let-7 was done using probes synthesized by Applied Biosystems. 100ng of RNA was used for TaqMan Synthesis using High capacity cDNA Reverse Transcription Kit (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... Assays were performed on a QuantStudio 7 Pro real-time PCR system (Thermo Fisher). Standard curves were generated using serially diluted pcDNA3.1 plasmids containing gN ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 µg of protein sample was loaded onto 7% Tris-Acetate gels (ThermoFisher #EA03552) with ice-cold 1X Tris-glycine buffer and run at 125V and 4°C for approximately 1 hr ...
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bioRxiv - Biochemistry 2023Quote: ... pH 8.0) with 7 kU Pierce Universal Nuclease for Cell Lysis (Thermo Fisher # 88702) and gentle end-over-end rotation for 15 min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... qPCR was performed using an ABI QuantStudio 7 Flex (Thermo Fisher, Waltham, MA, USA) with PowerUp SYBR Green Master Mix from Thermo Fisher (Cat ...
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bioRxiv - Neuroscience 2023Quote: ... Gates were validated by 7-AAD Viability Staining Solution (cat # 00-6993-50, Invitrogen) to identify live and dead cells ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 6µM CellEvent Caspase-3/7 Green Detection Reagent (Thermofisher, #C10423, green fluorescence). Cancer cell lines (IGR-Heu and IGR-Pub ...
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bioRxiv - Developmental Biology 2023Quote: ... Monocyte differentiation was induced from HSC for 5-7 days in RPMI (Gibco, #11875093), 10% FBS ...
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bioRxiv - Microbiology 2023Quote: ... QPCR reactions were performed using QuantStudio 7 Flex Real-Time PCR Systems (Applied Biosystems) and data was collected using the QuantStudio Real-Time PCR Software v1.3 ...
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bioRxiv - Immunology 2023Quote: ... qPCR was performed on a QuantStudio 7 Flex real-time PCR system (Applied Biosystems). mRNA expression data were normalised to HPRT and analysed by the comparative CT method ...
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bioRxiv - Cell Biology 2023Quote: ... The QuantStudio 7 Flex Real-Time PCR system (Applied Biosystems, Foster City, CA, USA) was used to measure gene expression levels ...
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bioRxiv - Cell Biology 2023Quote: ... and cut into 7 µm thin sections with a cryostat (Thermo Scientific, Cryostar NX70). Sections were then heated to 37°C for 8 minutes ...
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bioRxiv - Biochemistry 2023Quote: Thermal shift experiments were carried out using a QuantStudioTM 7 Flex system (Applied Biosystems) in a MicroAmp Optical 384-well plate (Applied Biosystems 4309849)57 ...
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bioRxiv - Biochemistry 2023Quote: ... 5-7 μg BACMID DNA was incubated with 4 μL Fugene (ThermoFisher, Cat# 10362100) in 200 μL of ESF 921 media for 30 minutes at 230C ...
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bioRxiv - Neuroscience 2023Quote: ... These assays were run in QuantStudio 7 Flex Real-Time PCR Systems (ThermoFisher Scientific). The Ct value was defined as the number of cycles required for the fluorescence to exceed the detection threshold ...
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bioRxiv - Immunology 2023Quote: ... Organoids (>passage 7) were passaged via single cell dissociation using 1x TrypLE Express (Gibco) and resuspended in Advanced DMEM/F12 (Gibco) ...
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bioRxiv - Bioengineering 2023Quote: ... media was buffered at a pH of 7 using 126 mM Na2HPO4 (Acros Organics) and 18 mM citric acid (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2023Quote: ... buffer exchanged into PBS using 7 kDa MWCO desalt spin columns (Thermo Scientific, #89882) and then measured for absorbance at 280 nm to determine its concentration ...
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bioRxiv - Microbiology 2023Quote: ... and either the QuantStudio™ 5 or QuantStudio™ Flex 7 analyser (Applied Biosystems). For analysis on viral gene copy numbers ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended in 500µl cold FACS buffer containing 1µl 7-AAD (Thermo Scientific) and filtered through a 40µm cap filter into FACS tubes ...
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bioRxiv - Biochemistry 2023Quote: ... with 8 kD dialysis membrane (SpectraPor 7 8000 Dalton MWCO; 11425919, Fisher Scientific UK). For syntaxin-6 the buffer used for dialysis was 10 mM Na-phosphate,150 mM NaCl ...
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The effect of single mutations in Zika virus envelope on escape from broadly neutralizing antibodiesbioRxiv - Microbiology 2023Quote: ... the transfection mixture was replaced with low-glucose DMEM supplemented with 7% FBS (Gibco BRI ...
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bioRxiv - Microbiology 2023Quote: ... 95% humidity and passaged every 7 days using 0.05% (v/v) Trypsin/EDTA (Gibco).
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bioRxiv - Immunology 2023Quote: ... EG.7 target cells were loaded with CellTracker™ Orange CMRA dye (ThermoFisher Scientific) for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... Real time polymerase chain reaction was performed on ViiA™ 7 system (Applied Biosystems). Total volume of qPCR reaction was 20µl and it contained 10µl of 2x qPCR Master Mix Low ROX (Eurogentec) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with 7-AAD viability staining solution (Thermo Fisher Scientific, Waltham, MA), fluorescein isothiocyanate (FITC)-labeled anti-mouse CD3ε antibody (BioLegend ...
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bioRxiv - Cell Biology 2023Quote: ... or CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit (Thermo Fisher Scientific) to label apoptotic cells (Caspase-3/7 activity-positive and SytoxAADvanced-negative ...
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bioRxiv - Cell Biology 2023Quote: ... Apoptotic cells were detected with CellEvent™ Caspase-3/7 Green Detection Reagent (Invitrogen) (1:500) ...
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bioRxiv - Developmental Biology 2024Quote: Nuclei suspensions were stained with 10 μg/mL 7-AAD (Invitrogen, Cat. No. A1310) and sorted on a BD Fusion Flow Cytometer (BD FACSMelody™4-Way Cell Sorter ...
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bioRxiv - Neuroscience 2024Quote: Intracellular H2O2 levels of astrocytes were determined using 2′,7′-dichlorodihydrofluorescein diacetate (DCFDA, Thermofisher). Primary cultured astrocytes were seeded onto 96-well black plates (ibid ...
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bioRxiv - Cell Biology 2024Quote: COS-7 cells were obtained from ATCC and grown in DMEM (Thermo Fisher Scientific) supplemented with 10% FBS ...
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bioRxiv - Biophysics 2024Quote: ... pH 8.0 using Zeba Micro Spin desalting columns (Thermo Scientific, USA, 7 kDa MWCO). For each measurement 4 µL of buffer exchanged and degassed protein sample was used ...
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bioRxiv - Molecular Biology 2024Quote: Transfected COS-7 cells and iPSC-derived RPE were dissociated with 0.25% trypsin (Gibco), and iPSCs with Versene ...
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bioRxiv - Neuroscience 2024Quote: ... using primer sets for target genes on a QuantStudio 7 Flex system (Applied Biosystems) and Bio-Rad CFX Opus 384 ...
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bioRxiv - Genetics 2024Quote: ... thermocycling was performed on a QuantStudio 7 Flex Real-Time PCR system (ThermoFisher Scientific). Relative expression was quantified using the ΔΔCt method relative to RPS18 ...
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bioRxiv - Microbiology 2024Quote: ... qPCR was performed on a QuantStudio 7 Flex real time PCR machine (ThermoFisher Scientific). Each sample was run in duplicate and relative quantification was performed by normalization to GAPDH.
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bioRxiv - Neuroscience 2020Quote: ... Plates were washed three times with Tris wash buffer and incubated for 1 hr at RT on a plate shaker with 25 μl of 2 μg/ml detection antibody (methyl Tau antibodies (INNOVAGEN) or BT2 (Thermo Fisher, cat. no. MN1010) both labeled with MSD Sulfo-Tag-NHS-Ester (Meso Scale Discovery ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Mature sensory neurons in 8-well μ-slide chambers were co-stained with 10 nM tetramethylrhodamine methyl ester (TMRM; Life Technologies, Grand Island, NY) and 100 nM MitoTracker Green FM (Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: All fly lines were maintained in mixed sex groups in bottles on JazzMix media or our own food made following the same recipe (brown sugar, corn meal, yeast, agar, benzoic acid, methyl paraben and propionic acid; Fisher Scientific, Whitby, ON, Canada) at 25°C ...
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bioRxiv - Biochemistry 2023Quote: ... Each well was then further passivated by adding 9 μL of a freshly prepared aqueous solution of methyl-PEG4-NHS-Ester (10 mg/mL, Thermo Fisher, Cat. No. 22341), followed by adding 1 μL of 1 M NaHCO3 (pH 8.5) ...
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bioRxiv - Cancer Biology 2024Quote: ... Firstly proteins were precipitated with 50 μL of acetonitrile followed by steroid extraction via liquid-liquid extraction with 1 mL tert-methyl butyl ether (MTBE, Acros Organics, Fisher Scientific, UK). The MTBE layer was then removed ...
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bioRxiv - Cell Biology 2019Quote: ... 2,7-Dichlorodihydrofluorescein diacetate (DCFH-DA),3,3’-dihexyloxacarbocyanine iodide [DiOC6(3)] and N-[4-[6-[(acetyloxy)methoxy]-2,7-difluoro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-,(acetyloxy)methyl ester (Fluo-4 AM) were purchased from Molecular Probes (Invitrogen, Eugene, OR, USA). Agarose was obtained from Lonza (Walkersville ...
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bioRxiv - Molecular Biology 2021Quote: Mitochondrial membrane potential of primary neurons was measured by fluorescent staining of Tetramethylrhodamine methyl ester (Image-iT™ TMRM Reagent, ThermoFisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... The mitochondrial membrane potential and dysfunctional mitochondria were measured by staining BM cells with Tetramethylrhodamine Methyl Ester (TMRM 500 nM, Thermo Fisher Scientific, Cat. No. T668), MitoTracker Green (100 nM ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were dialyzed overnight using 7 kDa MWCO membranes (Slide-A-Lyzer G2, Thermo Fisher) into 500 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... The PCR reaction was performed on a ViiA 7 Real-Time PCR System (Applied Biosystems). Samples were run in triplicate and analyzed using comparative CT (2-ΔΔCT ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative RT–qPCR was performed with Real-Time PCR System (Applied Biosystems, Quantstudio 7 Flex), using SYBR Green Master Mix (Applied Biosystems ...