Labshake search
Citations for Thermo Fisher :
1001 - 1050 of 2879 citations for 7 METHYL CAMPTOTHECIN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2024Quote: ... Reactions were run on the QuantStudio 6 or 7 (ThermoFisher Scientific, Waltham, MA) using the qPCR reaction settings as follows ...
-
bioRxiv - Microbiology 2024Quote: ... RT-qPCR and qPCR were then performed on a QuantStudio 7 Flex (ThermoFisher Scientific ...
-
bioRxiv - Microbiology 2024Quote: ... were then subjected to qPCR using the Applied Biosystems ViiA 7 (ThermoFisher Scientific) for 40 cycles using the following thermocycling parameters ...
-
bioRxiv - Neuroscience 2024Quote: ... and 7 μL SYBR Green qPCR master mix (Thermo Fisher, cat no 4472908). The following primers were used ...
-
bioRxiv - Neuroscience 2024Quote: ... qPCR was performed in a ViiA 7 Real-time PCR system (Applied Biosystems) with ChamQ SYBR® Color qPCR Master Mix Low ROX Premixed (Vazyme).
-
bioRxiv - Plant Biology 2024Quote: ... 62.5 μL of BD 7-AAD Staining Solution (Fisher Scientific, catalog number: BDB559925) was added and mixed ...
-
bioRxiv - Neuroscience 2024Quote: qRT-PCR was performed (ViiA 7 Real-Time PCR System, Applied Biosystems, USA), and data was collected using the QuantStudio software (Applied Biosystems ...
-
bioRxiv - Cell Biology 2024Quote: ... and/or Sept-7-YFP using Lipofectamine™ 3000 Transfection Reagent (ThermoFisher, L3000001). Cells were used 48 hr after transfection.
-
bioRxiv - Microbiology 2024Quote: ... 1 µl of template DNA and 7 µl of nuclease free water (Invitrogen). The reaction mixture was incubated and amplified using Rotor-Gene Q at 65°C for 20 min followed by melt curve analysis at 99-80°C with an increment of 0.2 °C/ s ...
-
bioRxiv - Developmental Biology 2023Quote: ... Proteins were transferred using the iBlot® 7-Minute Blotting System (Invitrogen, USA) and blocked with 5% non-fat dry milk for 1h at room temperature ...
-
bioRxiv - Immunology 2023Quote: ... was performed and analyzed in a ViiATM 7 machine and software (Life technologies). All oligonucleotides are listed in Table S6.
-
bioRxiv - Systems Biology 2023Quote: ... in PBS) and stained with 7-AAD (1 µM; Thermo Fisher Scientific, A1310). 120,000 nuclei were sorted using an SH800 sorter (Sony ...
-
bioRxiv - Microbiology 2023Quote: ... Samples were analyzed in a ViiA 7 Real-Time PCR System (Applied Biosystems). All primer pairs used had an efficiency between 98-103 % (data not shown) ...
-
bioRxiv - Microbiology 2022Quote: ... and QuantStudio 7 Flex Real-Time PCR system (Applied Biosystems; Thermo Fischer Scientific). The primers and probe sequences were designed in previous reports and are described in Supplementary Table 2 ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... 7-Diethylamino-3-(4'-Maleimidylphenyl)-4-Methylcoumarin (CPM) was purchased from Thermo Scientific Life Technologies (Grand Island ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... MCF-7-iRFP cells were cultured in FluoroBright DMEM growth medium (Gibco, USA) with 10% fetal bovine serum (Capricorn ...
-
bioRxiv - Genomics 2023Quote: ... Reactions were carried out on the QuantStudio 7 Flex System (Thermo Fisher Scientific). Expression levels were quantified by the 2−ΔΔCt method.
-
bioRxiv - Microbiology 2023Quote: ... supplemented with 7% fetal bovine serum (FBS)(Cat# 26140079, lot 2358194RP, ThermoFisher Scientific) and 100 U/mL penicillin-streptomycin (Cat# 15140–122 ...
-
bioRxiv - Cell Biology 2023Quote: ... Vectors were transfected into COS-7 cells using the Turbofect transfection reagent (ThermoFisher) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2023Quote: ... Reactions were run on a ViiA 7 real-time PCR system (Thermo Fisher). Primer sequences are listed in Table S7.
-
bioRxiv - Genetics 2023Quote: ... and (7) mounted in an anti-fade medium already containing Hoechst (Invitrogen #P36981). All of the following primary and secondary antibodies were diluted in 1XPBS containing 5% donkey serum ...
-
bioRxiv - Cell Biology 2023Quote: ... MCF-7 cells were transfected with MitoDendra2 plasmid DNA using Lipofectamine 2000 (Invitrogen). Selection pressure was provided by medium (DMEM-GlutaMax with 10% FBS ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cytotoxicity was assessed using the CellEvent Caspase-3/7 Green Detection Reagent (ThermoFisher) at 1.0 µM and gating on CD45-negative cells ...
-
bioRxiv - Molecular Biology 2023Quote: ... HRM was performed using a QuantStudio 7 Pro Real-Time PCR System (Thermofisher) and results interpreted using Design and Analysis Software 2.6 (Thermofisher ...
-
bioRxiv - Plant Biology 2023Quote: ... each device was placed in a GA-7 Magenta box (Fisher scientific, USA) with a customized watering system (Fig ...
-
bioRxiv - Biophysics 2023Quote: Gels were blotted for 7 min using an iBlot Gel Transfer Device (Invitrogen). All further incubation steps were performed under agitation at room temperature if not stated otherwise ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Unreacted materials were purified using Zeba 7 kDa desalting spin columns (ThermoFisher Scientific). Thin layer chromatography was used to confirm radiolabeling efficiency ...
-
bioRxiv - Biochemistry 2023Quote: ... subtilis biofilm-promoting medium (51mM potassium phosphate [pH 7]; Fisher Scientific, Waltham, MA), 1001mM MOPS (morpholinepropanesulfonic acid [pH 7] ...
-
bioRxiv - Bioengineering 2023Quote: ... and analyzed on Quantstudio 7 Flex Real-Time PCR system (Thermo Fisher 4485701), following manufacturer’s instructions with UNG incubation hold at 50 °C for 2 min ➔ Polymerase activation Hold at 95 °C for 2 min ➔ PCR (40 cycles ...
-
bioRxiv - Bioengineering 2023Quote: ... we transfected GFP-KDEL to COS-7 cells with Lipofectamine 3000 (Invitrogen, L3000) transfection reagent ...
-
bioRxiv - Biochemistry 2022Quote: ... and 2 µM CellEvent™ Caspase-3/7 Green Detection Reagent (ThermoFisher Scientific). Images were captured automatically every two hours for 48 hours using the IncuCyte™ S3 Live-Cell Analysis Instrument (Essen BioScience) ...
-
bioRxiv - Cell Biology 2023Quote: COS-7 cells (ATCC CRL-1651) were cultured in DMEM medium (ThermoFisher #61965026), supplemented with fetal bovine serum (ThermoFisher ...
-
bioRxiv - Genetics 2023Quote: ... qPCR was carried out on Viia 7 Real-Time PCR System (Applied Biosystems) using SsoAdvanced Universal SYBR Green Supermix (BioRad) ...
-
bioRxiv - Immunology 2023Quote: ... Samples were processed using the ViiA 7 Real-Time PCR system (Applied Biosystems). Cxcl9 expression was calculated by the division of Cxcl9 quantity mean expression with quantity mean expression of reference gene Gapdh ...
-
bioRxiv - Genomics 2023Quote: ... following the standard curve method on a QuantStudio 7 Flex machine (Thermo Fisher). A “minus RT” control was used to confirm removal of genomic DNA ...
-
bioRxiv - Cell Biology 2023Quote: ... and treated with 2 µM Caspase-3/7 Green detection reagent (C10423, Invitrogen) along with the specified compounds ...
-
bioRxiv - Biochemistry 2023Quote: MCF-7 breast cancer cells (ATCC HTB-22) were grown in MEM (Gibco) supplemented with 10% fetal bovine serum (VWR) ...
-
bioRxiv - Neuroscience 2023Quote: ... and dry organoids were embedded in 7 µl of cold GeltrexTM (Thermo Fisher). GeltrexTM was left to solidify as hanging drops on the inverted cell culture dish for 10 min at 37 °C ...
-
bioRxiv - Cancer Biology 2022Quote: ... cells were resuspended in 7 ml of Waymouth’s medium (Gibco, San Diego, CA) containing 2.5 % FBS ...
-
bioRxiv - Cancer Biology 2023Quote: ... or 5- (and -6)-chloromethyl- 2′,7′-dichlorofluorescein diacetate (CM-H2DCFDA, ThermoFisher, C6827), or hydroxyphenyl fluorescein (HPF ...
-
bioRxiv - Plant Biology 2023Quote: ... and 7 μL ddH2O using the ABI Step One (Thermo Fisher Scientific, USA) with two technical replicates ...
-
bioRxiv - Immunology 2024Quote: ... 5 µg of anti-CD8α APC-efluo780 (clone 53-6-7, eBioscience/Thermofisher) was injected intravenously (i.v. ...
-
The effect of single mutations in Zika virus envelope on escape from broadly neutralizing antibodiesbioRxiv - Microbiology 2023Quote: ... supplemented with 7% fetal bovine serum (FBS) (Gibco BRI; Life Technologies, Gaithersburg, MD) and 100 U/mL penicillin-streptomycin (Gibco BRI ...
-
bioRxiv - Molecular Biology 2023Quote: ... on a QuantStudio™ 7 Flex Real-Time PCR System platform (ThermoFisher Scientific). All experiments were carried out in triplicate technical repeats for three biological replicates ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells were cultured for 7 days on bacterial grade plates (Thermo Scientific, 101R20) at 37°C and 5% CO2 ...
-
bioRxiv - Bioengineering 2023Quote: ... Quantitative real time PCR was run on Applied Biosystems QuantStudio 7 Flex (ThermoFisher) using ∼200 ng RNA ...
-
bioRxiv - Cancer Biology 2023Quote: ... They were grown for 7 days in RPMI 1640 (Thermo Fisher Scientific #11875093) supplemented with 10% FBS (Thermo Fisher Scientific #10270-106) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Neurons were cultured for 7-10 days in complete Neurobasal Plus Medium (Gibco). Mixed glial cells were cultured with the same cortical cell isolation procedure ...
-
bioRxiv - Physiology 2024Quote: ... and quantified by the ViiA 7 Real-Time PCR System (Applied Biosystems, USA). The amount of FoxO1 and FoxO3 mRNA expression was normalized with endogenous control TFRH ...
-
bioRxiv - Immunology 2023Quote: ... cells were washed and stained with 7-Aminoactinomycin D (7AAD) (Invitrogen, 1:400) that was used as dead cell marker ...