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Citations for Thermo Fisher :
1051 - 1100 of 10000+ citations for SpectraDye Antibody Labeling Kit IR800 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ROS production from CD8+ T-cells following MDSC-derived exosomes treatment in vitro was estimated by labeling the CD8+ T-cells using CM-H2DCFDA (Invitrogen™, C6827). In short CD8+ T-cells were isolated according to the above-mentioned method ...
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bioRxiv - Biochemistry 2020Quote: ... Covalent labeling of amine groups of the proteins with Alexa Fluor 488 or Alexa Fluor 647 succinimidyl ester dyes (Molecular probes/Invitrogen) was conducted in its polymer-assembled form as previously described (González et al ...
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bioRxiv - Microbiology 2020Quote: ... Washed cell pellets were either fixed with 4% paraformaldehyde (PFA) in PBS for labeling with fluorophore Alexa Fluor 647 azide (AF647-azide, Invitrogen, Carlsbad, CA) or flash frozen with liquid nitrogen and stored at −80°C until additional processing for LC-HRMS analysis.
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bioRxiv - Microbiology 2020Quote: ... They were adjusted to a concentration of 1 × 106 bacteria / ml and equally mixed before labeling with the SYTO 62 DNA staining dye (Thermo Fisher Scientific) at 1 μM and the Gram-positive dye Vancomycin BODIPY™-FL at 2 μg/ml for 15 mn ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were supplemented with 150 fmol of internal standard (yeast ADH1, Waters) and subjected to tandem mass tag (TMT)-labeling using TMT 6-plex reagents (Thermo Fisher Scientific). TMT 126-128 were used for the reference (Vehicle or WT ...
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bioRxiv - Plant Biology 2022Quote: ... The subsequent proteolytic digestion and labeling using TMT 10-plex reagents were carried out according to the manufacturer’s recommended protocol (Thermo Scientific, MA, USA). The TMT 10-plex tagged tryptic peptides were fractionated using high pH reversed phase (hpRP ...
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bioRxiv - Neuroscience 2020Quote: ... TdT-mediated dUTP Nick-End Labeling (TUNEL) assay was performed using Click-iT Plus TUNEL Assay with Alexa Fluor 488 (Thermo Fisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed with ice-cold PBS-BSA before secondary labeling for 1 h at 4°C in 3 mL1:200 PE-conjugated streptavidin (Thermo Fisher S866) to label for bound ACE2 ...
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bioRxiv - Cancer Biology 2020Quote: Endogenous ROS levels of the cell lines were measured by labeling 5×105 cells for 30minutes at 37°C with redox-sensitive probes CellRox (5µM) (Life Technologies, NY, USA). Stained cells were washed twice with PBS and analyzed using Beckman Coulter Gallios flow cytometer (Becton Dickinson ...
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bioRxiv - Bioengineering 2019Quote: ... pCT302-TOM22 or pCT302-c-Kit yeast cells were labeled with varying concentrations of the biotinylated Sso7d or nanobody mutants followed by secondary labeling with a 1:250 dilution of streptavidin R-phycoerythrin conjugate (SA-PE; Thermo Fisher Scientific). Flow cytometry analysis was completed as previously described31 ...
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bioRxiv - Genomics 2019Quote: Forward and reverse strand of 21-mer DNA oligos encompassing rs398206 were synthesized with 5’ biotin labeling (Life Technologies; Supplementary Table 15) and were annealed to make double stranded probes ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were then resuspended in 50 μL of 50 mM HEPES buffer and isobarically labeled using 400 μg of Tandem Mass Tag 6-plex (TMT6) isobaric labeling reagent (Thermo Fisher Scientific). The labeling reactions were then quenched with 4 μL of 5% hydroxylamine ...
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bioRxiv - Biochemistry 2022Quote: ... Free dye was removed from labeled protein by buffer exchanging labeling reactions three times using a ZebaTM Spin Desalting Column (7 kDa MWCO, Thermo Scientific #89890) into 50 mM Tris-HCl (pH 7.9) ...
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bioRxiv - Microbiology 2022Quote: ... These LY2-specific probes were made by random priming and labeled with biotin using the BioPrime Array CGH Genomic Labeling System (Invitrogen catalog # 18095012). Membranes were incubated with IRDye800 and imaged using Odyssey DLx imaging system (LI-COR).
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bioRxiv - Neuroscience 2023Quote: Peptides were reconstituted in 100ul of 100mM triethyl ammonium bicarbonate (TEAB) and labeling performed as previously described (1, 2) using TMTPro isobaric tags (Thermofisher Scientific, A44520). Briefly ...
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bioRxiv - Plant Biology 2024Quote: ... transferred to a PVDF membrane and detected using Ponceau S staining or No-Stain™ Protein Labeling Reagent (ThermoFisher Scientific™, #A44449). Membrane blocking in 5% (w/v ...
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bioRxiv - Molecular Biology 2023Quote: ... we followed the same procedure as described earlier in the presence of LysoTracker ™ Blue DND-22 fluorescent acidotropic probes for labeling and tracking acidic organelles in live cells (L7525, ThermoFisher, USA). The staining was carried out according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: 1.5 µg trypsinized peptides were dissolved in 50 mM TEAB pH 8.5 and labelled using the TMT10plex Isobaric Labeling Reagent Set (Thermo Fisher Scientific, 90111). Each sample was labeled with TMT10plex reagents at a 20:1 label:peptide w/w ratio ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were resuspended in 50 µl of DPBS with 10% FBS and incubated with 2.5 µl of Vybrant® DiI cell-labeling solution (Invitrogen, MA, USA) for 20 min ...
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bioRxiv - Neuroscience 2023Quote: ... concentration 1:100) for 24 h at 4°C followed by labeling with goat anti-rabbit Alexa fluor 488 (A11034, Invitrogen, 1:1000) as a secondary antibody for 3 h at room temperature ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were again washed 3x with 1 mL DPBS for 5 min each followed by nuclear labeling for 5 min with 1 mL 300 nM DAPI dissolved in DPBS (Thermo Fisher Scientific) and a 5-min DPBS wash ...
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bioRxiv - Neuroscience 2023Quote: Site specific labeling of the engineered nanobody was done using maleimide Alexa Fluor™ 647 C2 or Alexa Fluor™ 488 (ThermoFisher). Briefly ...
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bioRxiv - Bioengineering 2023Quote: ... cells were suspended at a density of 1 × 106 cells ml−1 in serum-free culture medium and labelled with Vybrant® DiD (Cell-Labeling Solution, V22887, Molecular Probes) following manufacturer protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... was converted into cDNA with the Invitrogen SuperScript III system (18080-051) and subjected to real-time qPCR using the SYBR Green labeling method (Applied Biosystems; 4309155) on an Applied Bioscience QuantStudio thermocycler ...
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A conserved germline-specific Dsn1 alternative splice isoform supports oocyte and embryo developmentbioRxiv - Cell Biology 2024Quote: 1 µg trypsinized peptides were dissolved in 50 mM TEAB pH 8.5 and labelled using the TMT10plex Isobaric Labeling Reagent Set (Thermo Fisher Scientific, 90111). Each sample was labeled with TMT10plex reagents at a 20:1 label:peptide w/w ratio ...
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bioRxiv - Molecular Biology 2019Quote: ... secondary antibodies were visualized with an enhanced chemiluminescence detection kit (Thermo Scientific, Rockford, IL, USA). The band intensities were quantified using Gene Tools software (Sygene ...
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bioRxiv - Cell Biology 2021Quote: ... HRP-conjugated antibodies were detected with an ECL SuperSignal West Pico chemiluminescence kit (Thermo Scientific) ECL Supersignal West Pico ...
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bioRxiv - Immunology 2022Quote: ... The endotoxin levels of the purified antibodies were measured by Chromogenic Endotoxin Quant Kit (ThermoFisher) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... after antibody staining slides were stained using the Click-iT® EdU Imaging Kit (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... then with the Blocking Endogenous Antibody Technology (BEAT) kit (Invitrogen Corporation, Camarillo, CA; 50-300). Overnight incubation with the primary antibody (Thermo Fischer Scientific ...
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bioRxiv - Immunology 2019Quote: ... Laser compensation was performed using the AbC™ Total Antibody Compensation Bead Kit (Thermo Fisher). Single cells were sorted directly into Hard-Shell® 96-well ...
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bioRxiv - Physiology 2019Quote: ... AlexaFluor 594 was used to visualize antibody-binding for Pax7 (1:100, TSA kit, Invitrogen). Sections were mounted and nuclei were stained with Vectashield with DAPI (H-1200 ...
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bioRxiv - Immunology 2021Quote: ... which was conjugated to beads using the Dynabeads Antibody Coupling Kit per manufacture instructions (ThermoFisher). Exosomes were co-incubated with magnetic beads conjugated with a CD66b/CEACAM 8 antibody in a shaker for 18 h at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... and antibody concentration using a micro BCA protein assay kit (ThermoFisher Scientific cat. no. 23235), and was ∼80% with 1-2 DNA strands per antibody.
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bioRxiv - Molecular Biology 2023Quote: ... were covalently bound to 1mg magnetic epoxy beads (Dynabeads Antibody Coupling kit, Life Technologies #14311D), according to manufacturer’s protocols ...
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bioRxiv - Cell Biology 2023Quote: ... Incubation with the antibodies was performed using iBind Flex Solution Kit (SLF2020, Thermo Fisher Scientific), iBind Flex Cards (SLF2010 ...
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bioRxiv - Immunology 2023Quote: ... NAb™ Protein L Spin Kit for all Kappa chain antibodies (all from Thermo Scientific). Purified intact antibodies (40 μg IgG ...
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bioRxiv - Cell Biology 2023Quote: ... and blotted with anti-6xHis antibody (1:5000 dilution) followed by HRP-labeled secondary antibody and ECL reagents from the Pierce Fast Western kit (Thermo Scientific, Waltham, MA, USA). The bottom half was stained with SimplyBlue Safe stain (Life Technology ...
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bioRxiv - Microbiology 2020Quote: ... Two μg of either an equimolar mixture of the eleven Altivir_1_MSI polynucleotides or of the single negative control polynucleotide were used in a single labeling reaction and then purified using NucAway Spin Columns (Thermo Fisher Scientific, USA). Before being used as probes for virus-targeted genome fluorescence in situ hybridization (genomeFISH) ...
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bioRxiv - Neuroscience 2021Quote: ... The tip of the micropipette was coated with a fluorescence dye (Vybrant® Dil Cell-labeling solution, Life Technologies, reference V22885, MW: 933.88), filled with artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Biochemistry 2021Quote: ... The final 50 μL mixtures were individually labeled using 20 μg of a single channel of TMT10plex isobaric labeling reagent (Thermo Fisher Scientific PI90110) in anhydrous ACN and incubated for 1 h at RT ...
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bioRxiv - Neuroscience 2022Quote: ... probes were maximally lowered into an excess quantity of lipophilic dye for histological probe tracking (Vybrant DiI Cell-Labeling Solution, ThermoFisher Scientific, Waltham, MA) repeatedly for several seconds.
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bioRxiv - Pathology 2024Quote: ... Tandem mass tag (TMT)-based labeling of peptides was performed using the TMT10plex™ Isobaric Label Reagent Set (Thermo Fisher Scientific, Cat#90110) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Bipolar twisted pair platinum-iridium insulated electrodes (MS333/8-BIU/SPC, Plastics One, Roanoke, VA) coated with Vybrant® DiI cell-labeling solution (Invitrogen, Eugene, OR) were placed bilaterally in one of four regions of interest ...
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bioRxiv - Cell Biology 2024Quote: ... optionally followed by co-labeling with Cholesterol-Star488/ -StarOrange (Abberior 0206, 40 nM) for 10 min or ER-Tracker Red (Invitrogen E34250, 1 µM) for 30 min in cell culture medium at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... The cells were fixed in 4% paraformaldehyde and washed with PBS followed by the terminal deoxynucleotidyl transferase-dUTP nick end labeling (Click-iT™ Plus TUNEL Assay for In Situ Apoptosis Detection, Thermo Fisher Scientific), according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Viability was assessed 48h later after labeling cells with Annexin V APC (TONBObiosciences San Diego, California) on the ATTUNE NxT cytometer instrument (Life technologies Carlsbad, California) and analyses were performed using FlowJo software (v10.8.1 ...
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bioRxiv - Microbiology 2023Quote: AdV stocks were produced as above and used frozen (AdV5) or fresh (AdV40 and AdV41) for covalent labeling with AF488-NHS (Thermo Fisher Scientific; #A20000). Viruses were used at a concentration of at least 500 ng/µL (best 1000 ng/µL ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed with 1X PBS and treated as indicated in the figure legends in the following labeling media: A549 cells were cultivated in DMEM medium (Gibco™, A14430-01) supplemented with 25 mM unlabeled glucose or [U-13C6]-glucose and 4 mM [U-13C5]- glutamine or unlabeled glutamine and 10 % dFBS ...
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bioRxiv - Immunology 2021Quote: ... Antibody biotinylation was preform using an EZ-Link Micro NHS-PEG4 Biotinylation kit (ThermoFisher cat. 21955).