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Citations for Thermo Fisher :
951 - 1000 of 10000+ citations for SpectraDye Antibody Labeling Kit IR800 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... In brief pulse labeling with FM4-64 (2 μM FM464, Molecular Probes; made from a 2-mM stock in DMSO) was done for 5 min (time 0 ...
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bioRxiv - Molecular Biology 2023Quote: HeLa cells were cultured in DMEM for Stable Isotope Labeling with Amino acids in Cell culture (SILAC, Thermo Fisher Scientific) supplemented with 10% dialyzed FBS (MWCO 10 kDa ...
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bioRxiv - Neuroscience 2023Quote: ... This was followed by GFAP overnight incubation and subsequent labeling with anti-rabbit Alexa 488 (1:500; Invitrogen; cat # A11034). GFAP staining in retinal flat mounts was inconsistent within and between conditions and thus was not analyzed.
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bioRxiv - Cancer Biology 2023Quote: Rhodamine tubulin was generated by labeling tubulin with X-rhodamine SE (5(and-6) carboxy-X-rhodamine succinimidyl ester) dye (ThermoFisher). Labeling stoichiometry was determined using a molar extinction coefficient of 115,000 M-1 cm-1 for tubulin and 78,000 M-1 cm-1 for X-rhodamine [58] ...
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bioRxiv - Biochemistry 2023Quote: SILAC labeling was performed by growing cells for at least eight passages in lysine- and arginine-free SILAC medium (DMEM, Invitrogen) supplemented with 10% dialyzed fetal calf serum (Thermo Fisher) ...
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bioRxiv - Cell Biology 2023Quote: HM sEV uptake by Caco-2 cells was performed after labeling EVs with carboxyfluorescein succinimidyl ester (CFSE; Thermo Fisher Scientific) [29] ...
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bioRxiv - Immunology 2023Quote: ... CHO and G8 hybridomas cells were loaded respectively with 10 μM of the red cell-labeling agent DDAO (Molecular Probes) [ex/em 635/662] and 1 μM Vybrant CFDA SE [ex/em 492/517] in serum-free RPMI-1640 for 30 minutes at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... The c-Myc tag labeling solution was prepared by a 1:100 dilution of c-Myc Alexa Fluor 488 conjugate (ThermoFisher) in PBS containing 0.2% BSA ...
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bioRxiv - Biophysics 2023Quote: ... were then seeded into the microfluidic chips 24 hours before labeling and incubated at 37ºC and 5% carbon dioxide (Thermo Scientific Heracell 150i CO2 Incubator ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were adapted to labeling conditions by growth in this media supplemented with 15% dialyzed fetal bovine serum (Thermo Fisher) and 100 ug/mL Primocin (Invitrogen) ...
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bioRxiv - Biochemistry 2024Quote: ... Labeling of WT and p.R196H actin at cysteine–374 using N–(1–pyrene)iodoacetamide (Thermo Fisher Scientific, Waltham, MA, USA) followed established protocols (55).
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bioRxiv - Bioengineering 2024Quote: ... A secondary labeling step was performed with goat anti-mouse IgG (H+L) HRP conjugate according to manufacturer’s instructions (Thermo Fisher) to facilitate chemiluminescent detection ...
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bioRxiv - Neuroscience 2024Quote: ... to a concentration of 106 segments/ml and fluorescently labeled with the lipophilic dye DiI (Vybrant Cell-Labeling Solutions; Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Microglia were labeled for live assays prior to incorporation using a cell membrane dye (Vybrant Cell-Labeling Solutions, Life Technologies). A stably expressing mCherry cell line was utilized for live imaging endothelial cells that was previously developed using pAAVS1-P-CAG-mCh (Addgene) ...
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bioRxiv - Neuroscience 2024Quote: ... The silicone sealant was removed from the craniotomy and a Neuropixels 1.0 probe previously dipped in red dye (Vybrant DiD cell labeling solution, ThermoFisher Scientific) was connected via cable to the PXIe data acquisition system (National Instrument NI-PXIe-1071 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then centrifuged at 18,000 x g for 1 min and resuspended in 50 µL of LB before labeling with 25 µg/mL of AlexaFluor488 C5-maleimide (AF488-mal) (ThermoFisher) for 15 min at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... Protein-antibody conjugates were visualized using a chemiluminescence detection kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: ... Reactive clones were tested using the Pierce rapid antibody isotyping kit (Life Technologies). Only IgG heavy-chain subgroups were continuously expanded ...
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bioRxiv - Developmental Biology 2020Quote: ... Antibody signals were detected using the ECL kit (Cat #32106, Thermo Fisher scientific) and ChemiDoc− MP Imaging System (Bio-Rad).
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bioRxiv - Immunology 2021Quote: ... HI sera were tested using PrioCHECK PRRSV Antibody ELISA Kit (Thermo Fisher Scientific) for detection of PRRSV N protein-specific antibodies ...
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bioRxiv - Cell Biology 2019Quote: ... coupled to magnetic beads (Dynabeads Antibody Coupling kit, Life Technologies, prod. number 14311D). Beads were washed five times in Triton buffer and resuspended in 100 μl of Triton buffer containing 30 μg of proteinase K (Invitrogen ...
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bioRxiv - Immunology 2019Quote: ... that was fluorescently conjugated using the Alexa Fluor 488 Antibody Labelling Kit (Invitrogen). For the labelling of LN conduit paths fluorescently conjugated WGA was used as an anterograde tracer ...
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bioRxiv - Biophysics 2021Quote: ... both produced with the SiteClick Qdot 705 antibody labelling kit (Thermo Fisher Scientific) utilizing monoclonal anti-Flag M2 antibody from mouse (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... Compensation was done using AbC Total Antibody Compensation Bead Kit (Thermo Fisher Scientific) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... CellTrace™ CFSE Cell Proliferation Kit and Qdot605 anti-CD4 antibody (Thermo Fisher). Stained cells were acquired using LSR II flow cytometer (BD Biosciences ...
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bioRxiv - Bioengineering 2021Quote: ... Compensation was setup using AbC™ Total Antibody Compensation Bead Kit (#A10513, ThermoFisher). All antibodies were supplied by eBioscience (San Diego ...
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bioRxiv - Immunology 2020Quote: ... an antibody cocktail containing LIVE/DEAD Fixable Dead Cell Stain Kit (Thermo Fisher), and the surface antibodies listed in S1 Table were added for 30 minutes at 4°C ...
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bioRxiv - Genomics 2021Quote: ... Each was conjugated with Dylight288 and/or 650 antibody labelling kits (Thermo Fisher).
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bioRxiv - Biochemistry 2021Quote: ... Antibody digestion was performed using a Fab preparation kit (Thermo Fisher Scientific, USA) and the resulting Fab fragments were analyzed using SDS-PAGE ...
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bioRxiv - Immunology 2020Quote: ... Antibodies were isotyped using the Rapid ELISA Mouse mAbs Isotyping Kit (ThermoFisher Pierce) with anti-mouse heavy chain capture antibody (anti-IgG1 ...
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bioRxiv - Genomics 2024Quote: ... antibodies were then revealed through SuperSignal West Dura chemiluminescence kit (Thermo Scientific, 34076) and signals acquired in an iBright FL1500 Imaging System.
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bioRxiv - Bioengineering 2023Quote: ... Site-Click Antibody-Azido modification kits were purchased from Life Technologies (cat #: S20026). Dibenzocyclooctyne-sulfo-N-hydroxysuccinimidyl (DBCO-NHS ...
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bioRxiv - Cell Biology 2023Quote: ... or AlexaFluor647 using the rabbit specific Zenon Antibody Labelling Kit (Thermo Fisher Scientific) and following manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... Compensation was done using AbC Total Antibody Compensation Bead Kit (Thermo Fisher Scientific) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Compensation was performed using AbC™ Total Antibody Compensation Bead Kit (Thermo Fisher) and ArCTM Amine Reactive Compensation Bead (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: ... directly conjugated to Alexa fluor647(AF647, Invitrogen Alexa Fluor™ antibody labelling kit) for 45 min at 4°C ...
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Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)bioRxiv - Cell Biology 2023Quote: ... primary antibodies at 4°C in incubation buffer (TSB kit, Thermo Fisher Scientific). Mouse IgM and rabbit IgG were used as controls ...
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bioRxiv - Immunology 2024Quote: ... was fluorescently labelled using the Alexa Fluor 488 Antibody Labelling kit (Life Technologies)102 ...
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bioRxiv - Immunology 2024Quote: ... the purified antibodies were processed using the Pierce FAB preparation kit (Thermo Scientific) as described previously 18 ...
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bioRxiv - Molecular Biology 2020Quote: ... The membranes were blocked with 5% skim milk compound with TBST for 3h and incubated with corresponding primary antibodies and appropriate secondary antibodies before the protein bands were detected with ECL-chemiluminescent kit (ECLplus, Thermo Scientific). Antibodies against human CD11b ...
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bioRxiv - Neuroscience 2020Quote: ... to magnetic beads at a final concentration of 10 mg of antibody/ mL of beads was done following the Magnetic Dynabeads Antibody Coupling Kit from Invitrogen (14311D). Human tissue lysates were also prepared using the “Sequential Extraction of Proteins from Tissue” protocol as described above with addition of 10 mM iodoacetamide prior to homogenization ...
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bioRxiv - Molecular Biology 2019Quote: ... 100 µg of the antibody was covalently conjugated to 17 mg of magnetic beads using a Dynabeads Antibody Coupling Kit (Thermo Fisher). The H3-conjugated beads (1.2 mg of beads ...
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bioRxiv - Neuroscience 2020Quote: ... to magnetic beads at a final concentration of 10 mg of antibody / mL of beads was done following the Magnetic Dynabeads Antibody Coupling Kit from Invitrogen (14311D). Human tissue lysates were also prepared using the “Sequential Extraction of Proteins from Tissue” protocol as described above with addition of 10 mM iodoacetamide prior to homogenization ...
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bioRxiv - Physiology 2024Quote: Immunoprecipitation of proteins without the contaminant of antibody heavy and light chain through bead antibody conjugation was carried out using the Pierce Co-IP kit (Invitrogen #26149). Briefly ...
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bioRxiv - Bioengineering 2023Quote: ... Primary antibodies were detected by a horseradish peroxidase (HRP)-conjugated anti-rabbit antibody using an ECL kit (Thermo Fisher Scientific, US) and imaged using ChemiDoc TM Touch Imaging System (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... Initial isotyping of the antibodies in hybridoma supernatants was carried out using Pierce Rapid Antibody Isotyping Kits for mouse (Thermo Fisher). Antibodies were subsequently used as confluent supernatants for most techniques ...
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bioRxiv - Cell Biology 2020Quote: ... Purified monomeric α-Syn was dialyzed extensively in the phosphate buffered saline (PBS) followed by labeling with 3-fold molar of pH-Rodo Red or Alexa596 succinimidyl ester fluorescence dye (Thermo Fisher) for 4 h at room temperature ...
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bioRxiv - Physiology 2019Quote: ... 5 muscle samples 100 μg each corresponding to 5 different age groups and one separately prepared master reference sample were labeled with 6-plex tandem mass spectrometry tags using a standard TMT labeling protocol (Thermo Fisher). 200 femtomole of bacterial beta-galactosidase digest (SCIEX ...
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bioRxiv - Systems Biology 2019Quote: The digested proteins were desalted with an HLB SPE column before labeling with TMT 10plex reagent (Thermo Fisher Scientific, Massachusetts, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... as described by Pilobello et al.60 Reference sample was prepared by mixing equal amounts of protein from each sample and labeling with NHS-activated AF555 (Thermo Fisher). Printing ...