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Citations for Thermo Fisher :
1301 - 1350 of 10000+ citations for SpectraDye Antibody Labeling Kit IR800 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Primary unconjugated antibodies and their corresponding fluorophore-conjugated secondary antibodies are as follows: NRG-1 primary antibody (Invitrogen) with Alexafluor 647-conjugated secondary antibody (ThermoFisher) ...
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bioRxiv - Cell Biology 2019Quote: ... anti-TAC102 antibody is crosslinked to protein A / protein G beads from the Pierce™ Crosslink Magnetic IP kit (Thermo Scientific™). A mitochondrial enriched fraction is prepared by digitonin fractionation with 100 ml of procyclic form parasites (cell line ...
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bioRxiv - Microbiology 2021Quote: ... cells were stained with the anti-double-stranded (ds) RNA antibody (J2, Scicons) -previously biotinylated with EZ-Link™ Micro NHS-PEG4-Biotinylation Kit (ThermoFisher 21955) to increase specificity and sensitivity of the assay- and with the APC/Cy7 streptavidin antibody (BioLegend ...
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bioRxiv - Molecular Biology 2021Quote: ... for anti-eEF3 and anti-G-6-PDH secondary antibodies and developed with Pierce ECL plus western blotting substrate kit (Thermo Scientific 32132).
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bioRxiv - Neuroscience 2019Quote: Antibodies against the C-terminal CAAGNGamide sequence of the Lineus excitatory peptide were raised in rabbits (genscript) and the antibodies were cleaned from the blood serum with a Sulfolink™ Immobilization Kit (ThermoFisher Scientific) according to (32) ...
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bioRxiv - Neuroscience 2020Quote: The atoxic binding fragment of tetanus neurotoxin (HCT) was bacterially expressed and labelled with AlexaFluor647 antibody labelling kit (Life Technologies, A-20186) as previously outlined (Gibbs et al. ...
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bioRxiv - Cell Biology 2021Quote: ... Detection of EdU was performed prior to incubation with the primary antibodies using the Click-iT™ Plus EdU Alexa Fluor™ 488 Imaging Kit according to the manufacturer’s instructions (Thermo-Fisher Scientific). Chk1 primary antibody (Abcam ab32531 - 1/1000 ...
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bioRxiv - Microbiology 2022Quote: Biotinylation of HBs-S and HBs-L was performed using the Antibody Biotinylation Kit for IP (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... The final antibody concentration was determined by measuring the absorbance at 562 nm using Pierce™ BCA Protein Assay Kit (Thermo Scientific). Purified antibodies were stored at -80°C prior to use.
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bioRxiv - Microbiology 2021Quote: ... Neutrophils were labelled with 2.5 μg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G, clone 1A8, Biolegend, #127620 + DyLight550 Antibody Labelling Kit, ThermoFisher Scientific, #84530) 15 min prior to recordings ...
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bioRxiv - Microbiology 2021Quote: ... 15 μg Dylight488-conjugated anti-mouse CD31 (LEAF purified anti-mouse CD31, clone 390, Biolegend, #102412 + DyLight488 Antibody Labelling Kit, ThermoFisher Scientific, #53024) and 2.5 μg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G ...
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bioRxiv - Microbiology 2021Quote: ... 100 μg Dylight755-conjugated UEA-1 lectin (Unconjugated UEA-1 lectin, Vector Laboratories, #L-1060 + DyLight755 Antibody Labelling Kit, Thermofisher Scientific, #84538), 15 μg Dylight488-conjugated anti-mouse CD31 (LEAF purified anti-mouse CD31 ...
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bioRxiv - Microbiology 2021Quote: ... the murine vasculature was labelled with 15 μg Dylight488-conjugated anti-mouse CD31 (LEAF purified anti-mouse CD31, clone 390, Biolegend, #102412 + DyLight488 Antibody Labelling Kit, ThermoFisher Scientific, #53024) and the human vasculature with 100μg of Dylight755-conjugated UEA-1 lectin (Unconjugated UEA-1 lectin ...
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bioRxiv - Microbiology 2021Quote: ... 100 μg Dylight755-conjugated UEA-1 lectin (Unconjugated UEA-1 lectin, Vector Laboratories, #L-1060 + DyLight755 Antibody Labelling Kit, Thermofisher Scientific, #84538) and 50 μg of Hydrazide AlexaFluor633 were injected in the mouse tail vein to label and visualize the human vessels and the arteriole walls ...
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bioRxiv - Microbiology 2021Quote: ... 100 μg Dylight755-conjugated UEA-1 lectin (Unconjugated UEA-1 lectin, Vector Laboratories, #L-1060 + DyLight755 Antibody Labelling Kit, Thermofisher Scientific, #84538) were injected in the mouse tail vein to label and visualize the human vessels ...
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bioRxiv - Microbiology 2021Quote: ... and 2.5 μg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G, clone 1A8, Biolegend, #127620 + DyLight550 Antibody Labelling Kit, ThermoFisher Scientific, #84530) were injected to label and visualize the human vessels ...
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bioRxiv - Microbiology 2021Quote: ... and the human vasculature with 100μg of Dylight755-conjugated UEA-1 lectin (Unconjugated UEA-1 lectin, Vector Laboratories, #L-1060 + DyLight755 Antibody Labelling Kit, Thermofisher Scientific, #84538). Neutrophils were labelled with 2.5 μg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G ...
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bioRxiv - Immunology 2022Quote: ... were transfected with plasmids carrying J08 and 02M04 antibody heavy and the light chains with a 1:2 ratio using ExpiFectamineTM293 Transfection Kit (Thermo Fisher Scientific) as recommended by the manufacturer ...
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bioRxiv - Immunology 2022Quote: ... They were both determined to be of the IgG2a subclass by using the Pro-DetectTM Rapid Antibody Isotyping Kit-Rat (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2019Quote: ... Calbindin signal was amplified with anti-rabbit horseradish-peroxidase-conjugated secondary antibody and Alexa Fluor® 488 tyramide from Tyramide Signal Amplification Kit (Life Technologies) following manufacturer’s instructions and nuclei were stained with DAPI (0.2 mg/ml) ...
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bioRxiv - Cell Biology 2019Quote: ... For immunoprecipitation of endogenous B55α, B55α antibody (clone 2G9, Milipore) was covalently cross-linked to agarose beads using Pierce Crssolink IP kit (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2019Quote: ... (Amgen Inc.) was covalently attached to 60mg of Dynabeads in a volume of 6mL using the Dynabead® Antibody Coupling Kit (Life Technologies), according to the manufactory’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... antibodies overnight at 4°C.The sections were then washed with PBS and incubated with a biotinylated secondary antibody for 15 min from a Histostain Plus kit (Invitrogen, CA, USA). The sections were then washed and incubated with 3 ...
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bioRxiv - Cell Biology 2019Quote: ... CALX173ACE solubilized CX3CL1 was loaded into magnetic beads previously crosslinked to polyclonal anti-CX3CL1 antibody using an IP kit (Pierce, Thermo Fisher Scientific). Retained CX3CL1 was eluted by pH shock (basic ...
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bioRxiv - Biochemistry 2021Quote: ... Capture antibodies were coupled to Carboxylated Paramagnetic Beads from the Simoa Homebrew Assay Development Kit (Quanterix) using EDC chemistry (Thermo Fisher Scientific). Detection antibodies were conjugated to biotin using EZ-Link NHS-PEG4 Biotin (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... at 48 h post transfection and immunoprecipitated using HA tag antibody as per the instructions given in the Pierce Co-Immunoprecipitation kit (Thermo Scientific, USA).
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bioRxiv - Molecular Biology 2020Quote: The immune precipitated samples using HA antibodies were resolved by SDS-PAGE and the gel was silver stained using Pierce Silver Stain Kit (Thermo Scientific, USA). The distinct protein bands were excised and were subjected to in-gel trypsin digestion using In-Gel Tryptic Digestion Kit (Thermo Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... by immunoprecipitation in antibody (each 20 µg) immobilized agarose beads (each 50 µl) from Pierce™ Co-Immunoprecipitation Kit (Thermo Scientific™). For each assay ...
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bioRxiv - Immunology 2022Quote: ... with anti-His and anti-IL-15 antibodies and live/dead staining) using the LIVE/DEAD Fixable NearIR Dead cell stain kit (Thermo Fisher Scientific) for all flow cytometry analysis ...
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bioRxiv - Bioengineering 2024Quote: ... Capture antibodies (provided in Supplementary Table 1) were biotinylated using the EZ-Link micro Sulfo-NHS-biotinylation kit from (Thermo Fisher Scientific). The biotinylated antibodies were then diluted to 10 μg/mL in a 1 % BSA solution and added to the grids for 15 min ...
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bioRxiv - Synthetic Biology 2024Quote: ... as previously described [63] and neutrophils were labelled by were labeled with 2.5 µg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G, clone 1A8, Biolegend, #127620 + DyLight550 Antibody Labelling Kit, ThermoFisher Scientific, #84530). Mice were infected by intravenous injection of 100 µl of the bacterial inoculum (107 CFU total ...
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bioRxiv - Immunology 2024Quote: The sandwich ELISA assay was performed with 4 µg/ml coated F(ab’)2 fragments that were generated from full-size antibodies using the Pierce Mouse IgG1 Fab and F(ab’)2 Preparation Kit (Thermo Fisher, 44980) according to the manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... TNF-α and IL-5 cytokines using a mouse cytokine 11-plex antibody bead kit (Th1/Th2 Cytokine 11-Plex Mouse ProcartaPlex™ Panel, Cat No# EPX110-20820-901, Thermo Fisher). Preparation of samples ...
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bioRxiv - Molecular Biology 2023Quote: ... A cognate secondary antibody coupled to horseradish peroxidase was used and revealed with the Super Signal West Dura Extended Duration Substrate kit (Thermo Fisher Scientific). Acquisition was performed using the Fusion FX (Vilber ...
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bioRxiv - Cell Biology 2023Quote: ... We further purified the polyclonal antibodies using a PfSTART1 affinity column that was generated using the AminoLink® Plus Immobilisation Kit (Thermo Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... For quality controls, PSCs passed array CGH testing (Cell Line Genetics, Madison, WI) and pluripotency testing using a four-antibody kit (Thermo Fisher #A24881). Cell culture media were sterile filtered (Fisher Scientific #SCGP00525 and #0974063D) ...
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bioRxiv - Cell Biology 2020Quote: ... Antibodies used were rabbit polyclonal anti-GFP antibody (A-6455; Invitrogen); rabbit polyclonal anti-β-PIX antibody (4515S ...
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bioRxiv - Developmental Biology 2020Quote: ... Secondary antibodies included Alexa546 goat anti-mouse (red) antibody (Molecular Probes) and Alexa488 goat anti-rabbit (green ...
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bioRxiv - Microbiology 2022Quote: ... as the primary antibody followed by Alexa647-conjugated secondary antibody (Invitrogen). The monolayers were imaged using a BioTek Cytation3 instrument and virus foci (approximately 100-200/well in no-serum control wells ...
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bioRxiv - Molecular Biology 2020Quote: ... The secondary antibodies were Alexa Fluor conjugated goat antibodies (Thermo Scientific): α-mouse ...
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bioRxiv - Genomics 2020Quote: ... Primary antibodies were mouse anti-MF20 antibody (1:100, 14650382, Invitrogen) and rabbit anti-TMSB4X antibody (1:200 ...
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bioRxiv - Microbiology 2020Quote: ... rabbit α-RSV polyclonal antibody and HRP-conjugated secondary antibodies (Invitrogen). RSV antibodies were generous gifts from Rebecca Craven (Penn State College of Medicine) ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were then labeled using AlexaFluor-conjugated secondary antibodies (Invitrogen). Sections were visualized with a Zeiss Imager ...
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bioRxiv - Genetics 2019Quote: ... Secondary antibodies were goat anti-rabbit antibodies (ThermoFisher Scientific, 1:500) and goat anti-mouse antibodies (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were diluted in antibody diluent (003118, Thermo Fisher Scientific) and sections were incubated overnight at 4°C in a humidified chamber ...
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bioRxiv - Biochemistry 2020Quote: The following antibodies were used: anti-V5 antibody (Invitrogen R960-25), mouse anti-FLAG M2 antibody (Sigma F1804) ...
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bioRxiv - Neuroscience 2019Quote: ... Secondary antibodies (Alexa Fluor secondary antibodies, Thermo Fisher Scientific, Waltham, Massachusetts) were applied according to species specificity of primary antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... The corresponding secondary antibodies included: 488 Donkey anti-mouse antibody (Invitrogen), 596 Goat anti-guinea-pig antibody (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were probed with primary antibodies followed by AlexaFluor antibody (Invitrogen). In the case of anti-HA probing ...
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bioRxiv - Biophysics 2022Quote: ... with anti-GFP antibody (monoclonal antibody, Invitrogen A11120, clone 3E6, IgG2a) in the same buffer used to isolate mRNA in a microtubule pellet ...