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Citations for Thermo Fisher :
1201 - 1250 of 10000+ citations for SpectraDye Antibody Labeling Kit IR800 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... cells were stained with extracellular antibodies followed by intracellular staining of EdU using click-iT Plus EdU Alexa Fluor 488 Flow Cytometry Assay Kit (Invitrogen), according to the manufacturer’s instructions.
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bioRxiv - Genetics 2022Quote: ... The Flow Cytometry analysis panel consisted of the following antibodies: Live/dead fixable near-IR Dead cell stain kit L34975 (Invitrogen); 421 Rat Anti-Mouse CD196 (CCR6 ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmids encoding the heavy chain and light chain of antibodies were transfected into Expi293F cells using an Expifectamine 293 transfection kit (Gibco) in a 2:1 mass ratio following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Fab were generated from monoclonal antibodies using the Pierce Mouse IgG1 Fab and F(ab’)2 Preparation Kit (Thermo Fisher). Antibodies were first digested into Fab in a Zeba Desalt Spin Column (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... Levels of active Rac1 and Cdc42 were detected using Rac1 and Cdc42 specific antibodies from the active Rac1/Cdc42 pull-down kit (Cat# 16118/19, ThermoFisher). Levels of GST-Pak1-PBD protein were detected using Ponceau S staininbg of the membrane ...
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bioRxiv - Neuroscience 2024Quote: ... membranes were washed after secondary antibody incubation before protein detection using SuperSignal West Pico Chemiluminescence Detection kit (Thermo Fisher Scientific). Images were acquired with a Bio-Rad Chemidoc XRS+ gel imaging system and analyzed with ImageJ/Fiji software (National Institutes of Health) ...
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bioRxiv - Immunology 2024Quote: ... and immunoprecipitation was performed with 2μg of anti-survivin antibodies (RnD AF886) coupled to the Dynabeads Protein G Immunoprecipitation Kit (10007D, ThermoFisher Scientific), cross-linked with bis(sulfosuccinimidyl)suberate (A39266 ...
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bioRxiv - Microbiology 2024Quote: Plasmids encoding the heavy chain and light chain of the antibody were transfected at an equimolar ratio into Expi293F cells using ExpiFectamine 293 transfection kit (Gibco) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Direct labelling of antibodies was done using a Zenon™ Alexa FluorTM 594 Rabbit IgG Labelling Kit (# Z25307, ThermoFisher Scientific). DAPI was added to the BS in a concentration 1 µg/ml in the last incubation step ...
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bioRxiv - Bioengineering 2023Quote: ... The GDF-15 and GFP antibodies were biotinylated using an EZ-Link Micro NHS-PEG4-Biotinylation Kit from Thermo Fisher Scientific (21955 ...
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bioRxiv - Cancer Biology 2023Quote: ... The concentration of the antibody was estimated using a Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA, USA). Approximately 4 mg of antibody (2 mL of antibody ...
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bioRxiv - Genomics 2023Quote: ... and SXL (1:500, a gift from Fatima Gebauer) antibodies using the Western Breeze kit following the manufacturer’s protocol (ThermoFisher Scientific). We quantified the relative expression of protein for SXL using the gel analysis tool in ImageJ software following the website’s guidelines 90 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... incubated with Goat Anti-Rabbit IgG secondary antibody poly HRP (1:1, Alexa Fluor 488 Tyramide SuperBoost Kit, goat anti-rabbit IgG, Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Cells were subsequently stained with surface antibodies and then either fixed using 1% paraformaldehyde or permeabilized using a FoxP3/Transcription factor fixation/permeabilization kit (ThermoFisher). Samples undergoing permeabilization were then stained with intracellular antibodies ...
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bioRxiv - Molecular Biology 2023Quote: ... The concentrations of the conjugated oligonucleotide DNA and antibodies were quantified using a BCA Protein Assay Kit (Thermo Scientific 23225) and Nanodrop (Thermo Scientific).
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bioRxiv - Microbiology 2023Quote: ... Plasmids encoding the heavy chain and light chain of antibodies were transfected into Expi293F cells using an Expifectamine 293 transfection kit (Gibco) in a 2:1 mass ratio following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Three technical replicates of 100 µg protein from each biological replicate diluted with diluent supplied by the manufacturer were added to the antibody-coated wells (Ferritin Human ELISA Kit, Invitrogen). All the steps were carried out following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Antibody units were then calculated in μg/ml from the standard sera which was quantified using antibody quantification kits from Invitrogen for IgG (88–50400 ...
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bioRxiv - Neuroscience 2019Quote: ... Secondary antibodies (Alexa Fluor-conjugated secondary antibodies, Molecular Probes) were then incubated at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Fluorescent secondary antibodies (Alexa-Fluor-conjugated antibodies; Molecular Probes) used were ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibodies used were Alexa-conjugated goat antibodies (Invitrogen) and were used at 1:1000.
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bioRxiv - Genetics 2020Quote: ... Secondary antibodies were Alexa Fluor antibodies (1:500, Invitrogen). Images were collected on a Zeiss Axio Imager M1 and were processed using ImageJ and Adobe Photoshop.
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bioRxiv - Cell Biology 2021Quote: ... or anti-Phospho-FOXO3a-antibody (1:200; Invitrogen Antibodies), respectively ...
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bioRxiv - Immunology 2022Quote: ... Western blot antibodies - GAPDH antibody (ThermoFisher cat# MA5-15738); pAKT 473 (Cell Signaling ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies used were HRP-coupled secondary antibodies (Invitrogen) in a dilution of 1:10000 and Clarity Western ECL Substrate was used to visualize the signal ...
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bioRxiv - Molecular Biology 2023Quote: ... The primary antibody (6xHis tag monoclonal antibody; Invitrogen/ThermoFischer), secondary antibody (Polyclonal rabbit anti-mouse immunoglobulins/horseradish peroxidase ...
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bioRxiv - Plant Biology 2023Quote: ... antibodies and detected using fluorescently tagged secondary antibodies (Invitrogen, goat raised anti-mouse 488 and Anti-rabbit 555) ...
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bioRxiv - Developmental Biology 2023Quote: ... Secondary antibodies: Alexa Fluor conjugated secondary antibodies from Invitrogen (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Other antibodies included antibodies to PINK1 (Invitrogen #PA5-86941) and β-actin (Sigma #A5441).
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bioRxiv - Developmental Biology 2024Quote: ... Secondary antibodies: Alexa Fluor conjugated secondary antibodies from Invitrogen (Thermofisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... The enzyme-dye mixtures were purified according to protocol included with the Antibody Conjugate Purification Kit for 0.5-1 mg (Thermo Fisher Scientific). The buffer was replaced with 50 mM HEPES (pH 7 ...
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bioRxiv - Cell Biology 2020Quote: Apoptosis was assessed through the incorporation of BrdUTP at the sites of DNA damage and detected with an Alexa Fluor 488 dye–labeled anti-BrdU antibody using the APO-BrdU TUNEL Assay Kit (Invitrogen, A23210). At the desired endpoints ...
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bioRxiv - Immunology 2021Quote: ... and IP was performed with antibodies against survivin (RnD AF886) coupled to the Dynabeads Protein G Immunoprecipitation Kit (10007D, ThermoFisher Scientific), cross-linked with bis(sulfosuccinimidyl)suberate (A39266 ...
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bioRxiv - Molecular Biology 2021Quote: Fab preparation was performed using the same monoclonal antibodies used in ChIP experiments and the Pierce Mouse IgG1 Fab and F(ab’)2 Preparation Kit (Thermo Scientific), as described before27 ...
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bioRxiv - Genomics 2020Quote: ... Blotting with primary antibodies was carried out at 4°C overnight followed by incubation with the appropriate secondary antibodies and developed using chemiluminescent West Pico detection kit (Thermo Scientific). Western blot quantification was carried out by subtracting the background signal from the individual bands ...
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bioRxiv - Bioengineering 2021Quote: ... The surface concentration of the antibodies was quantified as previously described using a bicinchoninic acid assay (BCA) kit (Thermo Fisher 23227)36.
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bioRxiv - Microbiology 2022Quote: ... IP control antibody included IgG purified from normal rabbit serum with Melon Gel IgG Spin Purification Kit (Thermo Scientific, Rockford, IL). The Co-IPs performed in triplicate.
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bioRxiv - Neuroscience 2021Quote: ... Membranes were then incubated for 1 h at room temperature with secondary biotinylated antibodies or with the ABC kit (Cat. no. 32020, Thermo Fisher). SuperSignal West Femto (Thermo Scientific Cat n ...
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bioRxiv - Developmental Biology 2019Quote: ... Chemiluminescence detection was conducted with HRP-conjugated secondary antibodies and either SuperSignalTM West Dura or SuperSignalTM Femto kits (Pierce/Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... The chemiluminescent detection of the horseradish peroxidase on the secondary antibodies was detected by the Supersignal West Dura kit (Thermo Scientific). Images and of western blots were acquired on a ChemiDoc MP system (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein detection was facilitated using species-specific antibodies conjugated to horseradish peroxidase and Pierce® Peroxidase IHC Detection Kit (Thermo Scientific) or species-specific secondary antibodies with IRDye Fluorescent Dyes (IRDye 800CW goat anti-rabbit IgG ...
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bioRxiv - Immunology 2019Quote: ... Viability was determined using LIVE/DEAD Fixable Aqua Dead Cell Stain kit (DCM) for 405 nM excitation and biotin-conjugated antibodies were visualized using Streptavidin-QD705 (Life Technologies). Cells were fixed and permeabilized using Fixation/Permeabilization Kit (eBioscience ...
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bioRxiv - Immunology 2020Quote: ... membranes were incubated with the appropriate HRP-conjugated secondary antibodies and developed using an enhanced chemiluminescence detection kit (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2021Quote: ... was conjugated to AlexaFluor-488, and anti FOXC2 (Goat, R&D AF6989) was conjugated to AlexaFluor-647 using Molecular Probes Antibody Labelling Kit (Invitrogen/ ThermoFisher). When labelling OTX2 ...
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bioRxiv - Developmental Biology 2022Quote: ... EdU incorporation was determined subsequent to immune-staining with anti-YFP antibodies using Click-iT Edu Alexa Fluor 594 Imaging kit (Life Technologies).
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bioRxiv - Immunology 2022Quote: Preparation of F(ab’)2 fragments from the C03 monoclonal antibody was performed with a Pierce F(ab’)2 preparation kit according to the manufacturer instructions (ThermoFisher Scientific). Briefly ...
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bioRxiv - Immunology 2022Quote: ... samples were incubated with biotinylated secondary antibody and developed with HRP and substrate using a DAB substrate kit (Thermo Fisher Scientific). Primary and secondary antibodies used in this study are listed in Supplemental Table 2 ...
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bioRxiv - Developmental Biology 2023Quote: ... they were incubated for 10 min at RT with biotinylated polyvalent secondary antibodies (UltraVision Detection System HRP kit, Thermo Fisher Scientific). After three 5-min washes in PBS ...
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bioRxiv - Immunology 2022Quote: ... the eBioscience FoxP3/Transcription Factor Staining Kit (00-5523-00) was used per manufacturer’s instructions with intracellular antibodies: PE-conjugated RORγT (Invitrogen; 12-6981-82), PE-Cy7-conjugated FoxP3 (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were blocked again and incubated with 2-3 drops of an anti-mouse poly-HRP-conjugated secondary antibody from the Invitrogen Tyramide SuperBoost secondary staining kit (ThermoFisher, B40912). Samples were rinsed three times with 1X PBS and the tyramide working solution conjugated to AlexaFluor 488 was added and allowed to react for 20 minutes ...