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Citations for Thermo Fisher :
851 - 900 of 10000+ citations for SpectraDye Antibody Labeling Kit IR800 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... were coupled to magnetic beads by Dynabeads antibody coupling kit (Invitrogen) following the description in the manual ...
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bioRxiv - Biochemistry 2023Quote: ... using the SiteClick Antibody Azido Modification kit (Invitrogen, Cat. No. S20026). Briefly ...
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bioRxiv - Immunology 2023Quote: ... Compensation was performed using AbC Total Antibody Compensation Bead Kit (Invitrogen) or cells ...
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bioRxiv - Microbiology 2023Quote: Antibody concentrations were determined using the Qubit Protein Assay Kit (ThermoFisher). The GloMelt™ Thermal Shift Protein Stability Kit (Biotum ...
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bioRxiv - Cancer Biology 2023Quote: ... was conjugated using Alexa Fluor™ 647 Antibody Labelling Kit (ThermoFisher). 100mg of CD8 antibody at a concentration of 1mg/mL was cleaned twice using the Zeba™ Spin Desalting Columns ...
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bioRxiv - Developmental Biology 2022Quote: ... The purified antibody was coupled to beads using the Dynabeads™ Antibody Coupling Kit (Thermo Fisher Scientific; 14311D), using 7µg antibody per mg beads ...
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bioRxiv - Immunology 2023Quote: ... The idiotype antibodies and Fc- fusion protein were conjugated in house with Dylight650 antibody labelling kits (Thermo Fisher). T cell surface phenotype was assessed using the following antibodies:
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bioRxiv - Immunology 2023Quote: ... Antibodies manually conjugated were purchased in a BSA-AZIDE free format and conjugated using antibody labelling kit (Invitrogen). Fluorophores were bleached between each staining round using NaHCO3 (0.1 M ...
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bioRxiv - Neuroscience 2023Quote: ... primary antibodies were rat α-Kit (1:500, Rat α-Kit clone ACK4, ThermoFisher, product MA5-17836), mouse α-calbindin (1:2000 ...
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bioRxiv - Immunology 2021Quote: ... and evaluated for B cell activation by labeling 10 million cells with 0.5µg/ml Fura red dye (Invitrogen) in 2ml of RPMI at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: 3′-desthiobiotin labeling was carried following the manufacturer’s’ guidelines of Pierce™ RNA 3′ End Desthiobiotinylation (ThermoFisher, #20163). Briefly ...
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bioRxiv - Developmental Biology 2019Quote: ... with the cDNA synthesis and cRNA labeling reactions performed according to the two-cycle protocol provided by Affymetrix. Affymetrix high-density oligonucleotide arrays for Gallus gallus (GeneChip® Chicken Genome ...
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bioRxiv - Cell Biology 2019Quote: ... prior to staining with organelle labeling dyes or mounting directly in SlowFade Gold antifade reagent with DAPI (Invitrogen) if FBs transgenically expressed fluorescent-tagged proteins ...
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bioRxiv - Physiology 2020Quote: ... biotin-labeled RNAs were transcribed in vitro using the Biotin RNA Labeling Mix and T7 RNA polymerase (Ambion) and purified with the RNeasy Mini Kit (QIAGEN) ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed with ultra-purified water and labeled with No-Stain Protein Labeling Reagent (Thermo Fisher Scientific) according to kit instructions ...
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bioRxiv - Cell Biology 2020Quote: EVs derived from wildtype hMB cells were labeled (1:100) with Vybrant(tm) DiD Cell-Labeling Solution (Invitrogen) 20 min 37°C ...
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bioRxiv - Biophysics 2022Quote: ... Final protein concentration and labeling efficiency were determined spectrophotometrically according to the guidelines provided by the manufacturer (ThermoFisher). Labeling efficiencies were ~95% and ~75% for SET/TAF-Iβ and NPM respectively ...
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bioRxiv - Neuroscience 2022Quote: ... pH 7.6) and assessed by proteomic profiling using the TMT labeling system (Thermo Fisher Scientific, Waltham, MA, USA). The high-resolution mass spectrometer Q Exactive Plus (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: O-GlcNAcylation of TRPC6 was also assayed using Click-iT GlcNAz metabolic glycoprotein labeling reagents (ThermoFisher Scientific C33368). Lysates (200 μg protein ...
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bioRxiv - Biophysics 2020Quote: ... The final protein concentration and labeling ratio were measured using NanoDrop™ UV-Vis Spectrophotometer (Thermo Scientific™).
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bioRxiv - Neuroscience 2019Quote: ... our own preliminary studies and others24 have shown that maxillary molar labeling with the DiI paste Neurotrace (Invitrogen) results in positive cells in all branches of the trigeminal nerve ...
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bioRxiv - Neuroscience 2019Quote: TMT labeling was performed according to the manufacturer’s instructions using the TMT10plex Isobaric Label Reagent Set (Thermo Scientific). In brief ...
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bioRxiv - Genomics 2021Quote: 3’-desthiobiotin labeling was carried following the manufacturers’ guidelines of Pierce™ RNA 3’ End Desthiobiotinylation (ThermoFisher, #20163). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: The staining of MSC-EV preparations with PKH67 followed the manufacturer’s instructions for labeling EVs (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... total protein loading on the PVDF membrane was stained with No-StainTM Protein Labeling reagent (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... mixing with 2 million CD45.1+ T cells at 1:1 ratio and labeling with CellTrace Violet dye (Invitrogen), into Rag1−/− mice ...
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bioRxiv - Biophysics 2023Quote: ... Fluorophore-labeled tubulin was prepared by labeling the purified tubulin with Alexa-Fluor 647-NHS (Invitrogen, A-20006) [2] ...
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bioRxiv - Biochemistry 2023Quote: ... Total protein normalization has been performed by using No-Stain™ Protein Labeling Reagent (ThermoFisher Scientific, Cat.n. A44717). Images were acquired with a Chemidoc MP imaging system (Bio-Rad) ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then collected and stained with Vybrant™ CM-Dil Cell-Labeling Solution (ThermoFisher Scientific, Cat. # V22888) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: The DIBO labeling solution was prepared by dissolving 0.5 mg of DIBO-alkyne Alexa Fluor 555 dye (ThermoFisher) in dimethyl sulfoxide (DMSO ...
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bioRxiv - Neuroscience 2023Quote: ... double immunofluorescence labeling with detergent was performed on 50-µm sections with the anti-pTau mAb AT8 (ThermoFisher) plus the anti-mouse IgG H + L secondary antibody conjugated with Alexa Fluor 488 (ThermoFisher) ...
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bioRxiv - Biochemistry 2023Quote: ... Final concentrations and fluorescent labeling ratios were determined by UV absorption using NanoDrop™ One/OneC (Thermo Scientific) (extinction coefficient for tubulin ...
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bioRxiv - Cancer Biology 2024Quote: Protein synthesis was assessed using O-propargyl-puromycin Click-iT labeling according to the manufacturer’s instructions (ThermoFisher Scientific) (32,33) ...
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bioRxiv - Neuroscience 2020Quote: ... heads were pulverized in homogenization buffer and then incubated with antibody-coupled magnetic beads (Dynabeads Antibody Coupling Kit, Invitrogen) to immunoprecipitate ribosomal bound mRNA species ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were detected using primary antibodies against LC3B (LC3B Antibody Kit for Autophagy, Invitrogen™, L10382, Carlsbad, CA, USA), SQSTM1/ p62 (SantaCruz ...
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bioRxiv - Biochemistry 2019Quote: The media utilized for isotopic labeling was Eagle’s minimum essential medium (ATCC) supplemented with 15% dialyzed FBS (Thermo Scientific), 100 U/mL penicillin ...
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bioRxiv - Developmental Biology 2020Quote: ... The cleaned worms were soaked in 300 µl 6.7 mM Vybrant™ DiI Cell-Labeling Solution (Molecular Probes V22889) diluted in M9 with gentle inversion every 30 minutes away from direct light ...
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bioRxiv - Neuroscience 2020Quote: ... FITC and Rhodamine (Rh) labeling of LMW α-Syn was performed62 as per the manufacturer’s recommendations (Molecular Probes, USA). Post labeling of protein ...
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bioRxiv - Cell Biology 2021Quote: ... cells were primed in metabolic labeling medium [DMEM containing 4.5 g/L glucose without L-methionine and L-cystine (#21013024, Gibco) including 10% dialysed serum ...
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bioRxiv - Microbiology 2020Quote: ... The protoplast membranes were finally stained with the Vybrant™ Dil Cell-Labeling Solution (Molecular Probes, catalog number V22885) at 5 μM (30 min ...
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bioRxiv - Biochemistry 2021Quote: ... The fluorescent labeling of PlyCBWT and PlyCBR66E was performed using the manufacturer’s recommended guidelines (Thermo Fisher, cat. No. A20174). Analytical gel filtration was used to determine the multimeric nature of PlyCB and PlyCBR66E ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were then washed with ultra-purified water and labeled with No-Stain Protein Labeling Reagent (Thermo Fisher Scientific) according to kit instructions ...
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bioRxiv - Microbiology 2022Quote: ... A total of 5×105 naïve HEp-2 cells were stained with Vybran DIO Cell-Labeling Solution (0.005 μg/ml, Thermo fisher) for 20 min at 37 °C and washed with PBS ...
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bioRxiv - Cancer Biology 2020Quote: The trigger peptides required for the TOMAHAQ method were generated by labeling with TMT-super heavy tags (TMTsh, +235.18, ThermoFisher) on the AssayMAP Bravo ...
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bioRxiv - Biochemistry 2020Quote: The Dip1 construct used for site specific labeling with the cysteine-reactive Alexa Fluor 568 C5 maleimide (Thermo Fisher) had the six endogenous cysteine residues in Dip1 mutated to alanine and a single cysteine added to the short N-terminal polypeptide sequence on the end of the coding sequence as previously described (Balzer et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... B6013-1) and 10 μL of 1 mM Vybrant(R) Cell-Labeling DiO or DiR dye (ThermoFisher, cat. V22889) was added to 2 mL of dichloromethane (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2020Quote: ... RBCs were washed twice in phosphate-buffered saline (PBS) and then labelled with DiD Cell-Labeling Solution (Thermo Fisher). The labelled RBCs were then washed twice again ...
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bioRxiv - Cell Biology 2021Quote: ... Rates of AHA incorporation were determined by labeling the cells with Alexa Fluor® 488 DIBO alkyne (Invitrogen C10405), followed by imaging and quantification of the integrated intensity of the fluorophore on a single cell basis as detailed above ...
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bioRxiv - Cell Biology 2022Quote: ... dialyzed overnight in a Slide-A-Lyzer dialysis cassette (MWCO 10,000) before Alexa Fluor 488 or 647 maleimide labeling (Invitrogen). Protein concentrations were measured using Bradford assay kits (Biorad).
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bioRxiv - Cancer Biology 2022Quote: ... 250 ng of total RNAs with a RIN of ≥8 were labelled using the GeneChip Whole Transcript Express Labeling Assay and hybridized to GeneChip Mouse Gene 2.0 ST Arrays (Affymetrix) at 45 °C for 16 h (SACRI Microarray and Genomics Facility ...