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Citations for Thermo Fisher :
9951 - 10000 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: Cancer cells (1 million cells) grown on coverslips of 1.5 mm thickness (ThermoFisher Scientific, 12-542B) were first incubated with 100 nM of Alexa Fluor 488 tagged antibody-TCO in PBS containing 1% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2023Quote: ... Collected cells were centrifuged and the cell pellet was suspended in DMEM with 5% FBS (Invitrogen). Cells were allowed to adhere for 2 h at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were treated with TRI reagent (0.3-0.4mL per 1x105-107 cells) from Invitrogen (Carsbad, CA) and RNA extraction performed according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Prior to measuring the cell viability the cells were washed with MEM (Earles MEM from Gibco) at least two times ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were lysed using the Cells-to-CT 1-step Taqman Kit (Invitrogen, cat no. A25602) using the manufacturer protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then stained with LIVE/DEADTM fixable dead cell staining (Thermo Fisher Scientific; Cat #L23105) and PE anti-mouse H-2Kb/H2Db antibody (Clone 28-8-6 ...
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bioRxiv - Cell Biology 2023Quote: ... filtered (40 µm cell strainer) and 1 μM SYTOX Blue dead cell stain (Thermo Fisher Scientific) was added ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated (after the staining) for 15 min with Cell Mask (1:2000, Thermofisher, c10046) in the blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Hepatoma cell line HepG2/C3A cells was grown in Minimum Essential Medium (MEM) (Invitrogen, Karlsruhe, Germany) supplemented with 10% ultra-low IgG FCS (GE Healthcare) ...
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bioRxiv - Biophysics 2023Quote: ... viable cell counts and cell size were determined by trypan blue staining (Invitrogen, Thermo Fisher Scientific) followed by passage through a Countess IITM automated cell counter (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... viable cell counts and cell size were determined by trypan blue staining (Invitrogen, Thermo Fisher Scientific) followed by passage through a Countess IITM automated cell counter (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Raji cells and CHO cells were cultured in complete RPMI-1640 (Gibco, 10% FCS, 2mM GlutaMAX), and HEK293T cells ...
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bioRxiv - Neuroscience 2023Quote: ... the cell suspension was filtered with 40 μm and then 10 μm cell strainers (Fisher scientific), poured into two uncoated T-25 flasks (Sarstedt ...
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bioRxiv - Biophysics 2024Quote: ... and full-length MLC-5 were purified from mammalian cultured cells (FreeStyle 293-F cell, Invitrogen). For purifying hsDHC-1382k ...
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bioRxiv - Biophysics 2024Quote: ... Vaccine-inoculated cells and control cells were rinsed with phosphate-buffered saline (PBS, Gibco, Carlsbad, CA), and fixed with 4% paraformaldehyde (Sino Biological ...
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bioRxiv - Bioengineering 2024Quote: ... mechanically disrupted into single-cell suspensions through a 70 μm cell strainer (FisherbrandTM; Thermo Fisher Scientific), and suspended in complete RPMI 1640 medium (Gibco ...
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bioRxiv - Cell Biology 2024Quote: Non-neuronal cells were imaged using either culturing media or Live Cell Imaging buffer (Life Technologies), and cultured neurons were imaged in modified Tyrode Buffer (119mM NaCl ...
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bioRxiv - Cancer Biology 2024Quote: Mitochondria were isolated from GBM cells using the Mitochondria Isolation Kit for Cultured Cells (Thermo Scientific) following manufacturer’s directions ...
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bioRxiv - Cancer Biology 2024Quote: ... Red blood cells were then removed by resuspending cells in 2 mL lysis buffer (Gibco, A1049201) for 2 minutes at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... After co-culturing the targeted A375 cells and T cells in RPMI 1640 medium (Gibco, #11875093) for 6 h ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary endothelial cells were expanded briefly using endothelial cell media [Advanced DMEM (Thermo Fisher Scientific, #12491015), 20% FBS (Hyclone ...
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bioRxiv - Microbiology 2024Quote: The infected BmE cells and NbSPN14 transgenic cells’ total RNA was isolated using TRIzol regent (Invitrogen), and the mRNA in this preparation was then reverse transcribed into cDNA using the Hifair® AdvanceFast One-step RT-gDNA Digestion SuperMix for qPCR (Yeasen ...
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bioRxiv - Immunology 2024Quote: ... Gating was based on single cells followed by cell viability staining (65-0866-14, Thermo Fisher). All data analysis of cells and EVs were carried out with FlowJo (TreeStar ...
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bioRxiv - Immunology 2024Quote: ... and dead cells were excluded using LIVE/DEAD™ Fixable Green Dead Cell Stain Kit (Invitrogen).
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bioRxiv - Immunology 2024Quote: ... Dead cells were excluded using the LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit (Invitrogen). The cells were analyzed using a spectral flow cytometer SONY SA3800 (Sony Biotechnology).
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bioRxiv - Microbiology 2024Quote: ... Dead cells were then stained using the Live/Dead Fixable Aqua Dead Cell Stain Kit (Thermofisher). After a 30-minute incubation ...
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bioRxiv - Biochemistry 2024Quote: ... cells were seeded (2 x 105 cells per well) in 35-mm glass bottom dishes (Nunc). Cells were initially starved with DMEM without FBS supplemented for 4-6 hours incubated at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were harvested at room temperature and counted using the Countess II automated cell counter (Invitrogen). 100,000 cells were used per reaction ...
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bioRxiv - Genomics 2024Quote: ... HEK293T cells were seeded into 6 well Nunc™ Cell-CultureTreated Multidishes (Thermo Scientific, Catalog# 140675) and incubated overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... All cells were maintained in stem cell culture media (SCM): Advanced DMEM/F12 (Gibco CAT# 12634028), Glutamax (Gibco ...
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bioRxiv - Bioengineering 2024Quote: ... cells were dissociated and stained with LIVE/DEAD fixable near-IR dead-cell stain (L10119, Invitrogen) and immunostained with PerCP/Cy5.5-conjugated anti-Annexin V (Biolegend ...
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bioRxiv - Cell Biology 2024Quote: Skeletal muscle or cells were lysed with protein extraction reagent (NP40 Cell Lysis Buffer; Thermo Fisher) in the presence of a protease inhibitor (Cat# 8340 ...
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bioRxiv - Immunology 2024Quote: ... GNS cell lines were cultured in StemPro Neural Stem Cell medium (StemPro NSC, Thermo Fisher Scientific) in flasks ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293T cells and PANC-1 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, 21041025) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... islets were dispersed into single cells using an enzyme-free Hanks’-based cell dissociation buffer (Gibco) and plated into 35 mm culture-treated Petri Dishes (Corning ...
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bioRxiv - Microbiology 2024Quote: ... Cell pellets were resuspended in a cell lysis buffer containing 0.5 mL TE buffer (Invitrogen; 12090015) along with 25 mg lysozyme (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... Cell concentration and viability was determined using the Countess II Automated Cell Counter (Thermo Fisher Scientific). Appropriate cell suspension volume (target recovery of 10,000 live cells ...
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bioRxiv - Neuroscience 2024Quote: ... N42 cells were seeded at a density of 12,000 cells/well on chamber slides (Thermo Scientific) and cultured as described above ...
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bioRxiv - Neuroscience 2024Quote: ... Cell viability was measured for each sample preparation using a Countess II automated cell counter (ThermoFisher) and was between the range of 67.5% to 82.3% before processing with the 10x Genomics platform63 ...
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bioRxiv - Neuroscience 2024Quote: ... cells pre-incubated in fresh E8 Complete media with 1x Revita Cell Supplement (Gibco #A26445-01) for 2 hours were harvested by enzymatic dissociation using StemPro Accutase (Gibco ...
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bioRxiv - Immunology 2024Quote: All cells were stained with LIVE/DEAD Fixable Blue Dead Cell Stain Kit (Thermo Fisher Scientific), diluted 1:1000 in 1x PBS ...
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bioRxiv - Neuroscience 2024Quote: ... for 1 L cultures of HEK293F cells (∼2-3x106 cells/mL) in Freestyle 293 media (Gibco) supplemented with 2% FBS (Gibco) ...
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bioRxiv - Immunology 2024Quote: ... Cell viability was evaluated using LIVE/DEAD fixable blue dead cell stain kit (Thermo Fisher, L23105). For intracellular cytokines ...
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bioRxiv - Neuroscience 2024Quote: ... cells were first stained with the LIVE/DEAD fixable aqua dead cell stain kit (Invitrogen L34966), followed by anti-CD8 (PE-Cy7 ...
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bioRxiv - Immunology 2024Quote: ... we also stained cells with the Live/Dead Fixable Aqua Dead Cell Stain (Thermo Fisher Scientific) for 20 minutes on ice to identify the live cell population ...
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bioRxiv - Immunology 2024Quote: ... and the cell pellet was resuspended in 500 µL red blood cell lysis buffer (Invitrogen, #00430054) and incubated on ice for 5 min ...
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bioRxiv - Microbiology 2024Quote: ... cells were stained with LIVE/DEAD™ Fixable Near-IR Dead Cell Stain (Thermo Fisher Scientific) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were incubated for 48h and then whole-cell RNA was extracted using TRIzol reagent (Invitrogen) followed by two chloroform washes ...
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bioRxiv - Microbiology 2024Quote: ... Cells were stained with a viability marker (LIVE/DEAD fixable blue dead cell stain kit, Invitrogen) and surface and intracellular markers ...
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bioRxiv - Microbiology 2024Quote: ... Cells were stained with a viability marker (LIVE/DEAD fixable blue dead cell stain kit, Invitrogen) and surface and intracellular markers ...