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Citations for Thermo Fisher :
9601 - 9650 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Red blood cells in the cell suspension were lysed by ACK Lysing buffer (Gibco, A10492-01) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... Live and dead cells were discriminated by Live/Dead Fixable Aqua Dead Cell Stain Kit (Invitrogen).
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bioRxiv - Molecular Biology 2022Quote: ... 293T cells expressing scFv on the cell surface were detached with trypsin-EDTA (Thermo Fisher Scientific) and washed twice in prechilled sort buffer (1% FCS ...
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bioRxiv - Microbiology 2022Quote: THP-1 cells were diluted to 5 x 105 cells/mL in RPMI + 10% FCS (Gibco) and treated with 100 nM phorbol 12-myrystate-13-acetate (PMA ...
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bioRxiv - Genetics 2022Quote: ... Cells were prepared using the ViewRNA Cell Plus Assay Kit (Fisher Scientific, Cat#: 88-19000-99) according to the manufacturer’s protocol with the minor alteration of fixation and permeabilization using 3:1 methanol:glacial acetic acid at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: Cells were loaded with cell permeant intracellular Ca2+ flux indicator Fluo-4AM in HBSS (Gibco#14175) for 30 min following manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2022Quote: Primary cells and cell lines were cultured in RPMI-1640 (Thermo Fisher Scientific, Waltham, MA, USA) medium supplemented with 10% FCS ...
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bioRxiv - Genetics 2022Quote: Cells were lysed and proteins were extracted from fresh fibroblast cells with RIPA buffer (Thermo Scientific) supplemented with protease and phosphatase inhibitor mini-tablet (Pierce ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were labelled with Dead Cell Apoptosis Kit with Annexin V FITC and PI (Thermo Fisher) or fixed in 70% ethanol at 4°C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were pre-fixed in 4% PFA+0.25% glutaraldehyde in Live Cell Imaging Buffer (Life Technologies) then washed before fluorescence light microscopy imaging ...
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bioRxiv - Cell Biology 2022Quote: ... The cell suspension was filtered twice in a prewet 70 μm cell strainer (#15370801, Fisher Scientific), the rubber tip of a 1ml syringe was used to facilitate the filtering ...
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bioRxiv - Neuroscience 2020Quote: ... and cell count and viability were determined using the Countess Automated Cell Counter (Invitrogen, ThermoFisher Scientific). Cells were pelleted ...
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bioRxiv - Neuroscience 2020Quote: ... and cell count and viability were determined using the Countess Automated Cell Counter (Invitrogen, ThermoFisher Scientific). Cells were pelleted ...
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bioRxiv - Neuroscience 2020Quote: ... starting with 9,000 iPSCs dissociated into single cells using StemPro Accutase Cell Dissociation Reagent (Life Technologies) per each well in U-shaped low attachment 96-well tissue culture plates (Corning ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were dissociated into a single cell suspension and maintained in Neurobasal Media (Thermo Fisher Scientific) supplemented with L-glutamine (5 mM) ...
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bioRxiv - Microbiology 2020Quote: ... the rates of cell-cell fusion were evaluated using a fluorescence microscope (EVOS M5000, Life Technologies). Five fields for microscopic analysis were randomly selected in each treated group ...
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bioRxiv - Biophysics 2020Quote: ... Vero E6 cells (10,000 cells/well) were plated in a 96 well plate (Nunc, Thermo Scientific) the day before the neutralization assay ...
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bioRxiv - Biophysics 2020Quote: ... Vero E6 cells (10,000 cells/well) were plated in a 96 well plate (Nunc, Thermo Scientific) the day before the neutralization assay ...
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bioRxiv - Neuroscience 2020Quote: Cell surface proteins were purified using the Pierce Cell Surface Protein Isolation Kit (Thermo Fisher Scientific) based on manufacturer instructions with a few modifications ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were incubated for 15 min with LIVE/DEAD Fixable Violet Dead Cell Stain (ThermoFisher Scientific) and a maximum of 2×106 cells was used for EdU staining using the Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay according to manufacturer’s instructions (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... The number of cells was counted using an automated cell counter (Countess; Invitrogen, Carlsbad, CA, USA).
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bioRxiv - Immunology 2020Quote: ... cells were resuspended to 10×106 cells/mL in staining buffer containing 5μg/mL Hoechst (Invitrogen) and 1μg/mL 7-AAD (Biolegend ...
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bioRxiv - Bioengineering 2021Quote: ... cells were seeded at 2 × 105 cells/well in round-bottom 96-well plates (Fisher Scientific) in IMDM with 10% FBS and 2% penicillin/streptomycin (Life Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: ... cells from monocultures or co-cultures of differentially labelled cells were harvested with 0.25% Trypsin (ThermoFisher) and resuspended in PBS with 0.1 μg/mL DAPI (Sigma) ...
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bioRxiv - Cancer Biology 2020Quote: Sorted cells were processed using Cell-to-CT 1-Step Power SYBR Green kit (Invitrogen, A25600) and primers from Supplementary Table 1 ...
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bioRxiv - Biochemistry 2021Quote: ... The number of cells per well was quantified using the CyQuant Cell Proliferation Assay Kit (Invitrogen) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Dead cells were identified using LIVE/DEAD Fixable Near IR Dead Cell Stain Kit (#L34975 Invitrogen). Cells were then acquired using a flow cytometer (Cytoflex ...
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bioRxiv - Cancer Biology 2021Quote: Flow sorted tumor cells were stained with Trypan blue and Countess II Automated Cell Counter (ThermoFisher) was used to assess both cell number and viability ...
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bioRxiv - Bioengineering 2021Quote: ... The tumor cells were pre-labeled using green cell tracker dye (CellTrace CFSE, Thermo Fisher, USA), which could be distinguished from the red FMN ...
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Activation of endoplasmic reticulum stress via clustering of the inner nuclear membrane protein SUN2bioRxiv - Cell Biology 2022Quote: ... U-2 OS cells (ATCC® HTB-96™) and HEK293FT cells (Thermo Fisher Scientific, R70007) were grown in DMEM containing 10% fetal bovine serum (FBS) ...
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bioRxiv - Microbiology 2022Quote: Samples from infected cells and cell culture supernatants were extracted using TRIzol reagent (Thermo Fisher Scientific). At day one and six post-infection ...
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bioRxiv - Immunology 2022Quote: ... dead cells were excluded with the LIVE/DEAD Fixable Blue Dead Cell Stain Kit (Thermo scientific). For intracellular ARG1 and RELMα staining ...
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bioRxiv - Cancer Biology 2022Quote: Total cell lysates were prepared using the Thermo Fisher Scientific Cell Lysis Buffer (Thermo Fisher Scientific) supplemented with a protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... Proliferation was assessed by incubating the cells with CellTrace™ Violet Cell (C34571, Invitrogen, 5 μM) for 20 minutes prior to seeding the cells onto OP9-DL1 stromal cells ...
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bioRxiv - Immunology 2022Quote: ... 100,000 NK cells were labeled with cell trace violet 1:500 (ThermoFisher Scientific, Waltham, MA, USA), and co-cultured at a 2:1 ratio with A549 targets for 4-hours in a 48 well plate ...
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bioRxiv - Cell Biology 2022Quote: ... resuspended to 1 × 106 cells/ml and labeled with Cell TrackerTM Deep Red (1.5 mM, Invitrogen) for 20 min at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... The cell cycle was examined after treating the cells with RNAse and with propidium iodide (Invitrogen). To evaluate the proliferative activity of Caco-2 cells ...
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bioRxiv - Immunology 2019Quote: ... total CD4+ T cells were isolated using the Dynabeads CD4 positive T cell isolation kit (Invitrogen). Purified cells were 95%–98% pure as determined by flow cytometry analysis ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were seeded at 3×103 cell/cm2 and maintained in LHC-9 media (ThermoFisher 12680013) at 37 °C with 85% humidity and 5% CO2 ...
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bioRxiv - Immunology 2020Quote: Target P815 cells were stained for 30 min with Cell Tracker green CMFDA (Thermo Fisher Scientific) and coated with 10 µg/ml anti-CD3 (eBiosciences ...
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Biosynthesis of Circular RNA ciRS-7/CDR1as Is Mediated by Mammalian-Wide Interspersed Repeats (MIRs)bioRxiv - Molecular Biology 2019Quote: ... the cells were trypsinized and filtered through a 40 μm Corning cell strainer (Thermo Fisher Scientific), seeded onto a 10-cm culture plate ...
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bioRxiv - Genomics 2019Quote: ... KH2 embryonic stem cells (ESCs) [23] were cultured on irradiated feeder cells in KO-DMEM (Invitrogen) supplemented with L-glutamine ...
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bioRxiv - Synthetic Biology 2020Quote: The indicated target cells were stained with Cell Trace Yellow following the manufacturer’s recommended protocol (ThermoFisher), and 10,000 target cells per well were co-cultured with 50,000 SNAP-CAR T cells (E:T=5:1 ...
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bioRxiv - Molecular Biology 2019Quote: ... transfected cells were incubated in cell dissociation buffer with 0.25% Trypsin-EDTA (Life Technologies, 25200-056). Clumps were then broken up by pipetting with the addition of complete media ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were washed with warm PBS and then cells were detached using TrypLE express 1X (ThermoFisher). Detached cells were then washed with ice cold PBS ...
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bioRxiv - Immunology 2019Quote: ... LIVE/DEAD Fixable Dead Cell Stain was used to exclude dead cells (ThermoFisher Scientific, Waltham, MA). Samples were run on a LSRFortessa (BD Bioscineces ...
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bioRxiv - Genomics 2019Quote: ... after which cells were frozen at 5 × 106 cells/mL in 5% DMSO (Thermo Fisher Scientific). For the MPRA ...
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bioRxiv - Cancer Biology 2019Quote: Single-cell suspensions were stained with Hoechst and Propidium Iodide (ReadyProbe Cell Viability Imaging Kit, Invitrogen) for 10 min at room temperature and cell numbers and viability were checked with a Countess automated cell counter (Thermo Fisher) ...
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bioRxiv - Cell Biology 2019Quote: ... cell-cell junctions were labeled with CellMask Deep Red plasma membrane stain (Molecular Probes, Life Technologies). Cells were imaged in the 561 and 647 channel every 35 seconds ...
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bioRxiv - Cell Biology 2019Quote: ... cell-cell junctions were labeled with CellMask Deep Red plasma membrane stain (Molecular Probes, Life Technologies). Cells were imaged in the 561 and 647 channel every 35 seconds ...