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Citations for Thermo Fisher :
9601 - 9650 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... Cells were stained with LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit (Thermo Fisher), followed by surfacing staining with antibodies in PBS with 2% FBS and intracellular staining according to the manufacturer’s protocols with eBioscience™ Foxp3/Transcription Factor Staining Buffer Set (Invitrogen) ...
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bioRxiv - Genomics 2023Quote: ... 1 million cells were collected and counted using the automated Countess II cell counter (ThermoFisher). Each cell pellet was washed with PBS ...
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bioRxiv - Cancer Biology 2023Quote: Prostate cancer cell lines were dyed with the blue CMAC live-cell dye (Thermo Fisher Scientific #C2110 ...
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bioRxiv - Cancer Biology 2023Quote: Cell viability was analysed using the Alamar Blue™ cell viability reagent (Thermo Fisher Scientific). After the post treatment incubation tumour spheres were washed with sterile phosphate buffered saline (PBS) ...
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bioRxiv - Cancer Biology 2023Quote: Cell viability was assessed using the Alamar Blue™ cell viability reagent (Thermo Fisher Scientific). Following exposure at specific time points (48 or 144 hours) ...
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bioRxiv - Cancer Biology 2023Quote: ... and cell viability was assessed by applying the PrestoBlue™ HS cell viability reagent (Invitrogen) in accordance with the manufacturer’s instructions [9] ...
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bioRxiv - Cancer Biology 2023Quote: ... single cells were stained with LIVE/DEAD Fixable Yellow Dead Cell Stain (Thermo Fisher #L34967), blocked with anti-CD16/32 (Biolegend #101330) ...
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bioRxiv - Cancer Biology 2023Quote: ... NK-92 cell line cytotoxicity was validated using PrestoBlue cell viability dye (Thermo Scientific, A13261) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... The number of cells in the wells was counted using Luna Cell Counter (ThermoFisher Scientific) with Trypan blue staining ...
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bioRxiv - Cell Biology 2023Quote: ... Dead cells were detected with a ReadyProbes™ Cell Viability Imaging Kit Blue/Green (Invitrogen): after the end of experiments ...
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bioRxiv - Immunology 2023Quote: ... Pheonix cell and LS174T cells were cultured in complete DMEM (Gibco, 10% FCS, 2mM GlutaMAX).
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bioRxiv - Cell Biology 2023Quote: ... with cells in medium first pelleted with adherent cells harvested with TrypLE express (Gibco, 12604021). Cell pellets were then stained utilising protocol seen above under Immunocytochemistry with cells stained with cleaved caspase-3 rabbit polyclonal primary antibody for 1 hour (Cell Signalling Technology ...
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bioRxiv - Cell Biology 2023Quote: ... islets were dispersed into single cells using Ca2+-free cell dissociation buffer (Thermo Fisher Scientific) supplemented with containing 10% (v/v ...
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bioRxiv - Neuroscience 2024Quote: SH-SY5Y cells were plated (100,000 cells/well) on 6-well plates (ThermoFisher Scientific, 353046) and allowed to adhere for 24 h ...
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bioRxiv - Cancer Biology 2024Quote: ... Data were normalized by cell number using the CyQUANT Cell Proliferation Kit (Thermo Fisher Scientific). For all measurements ...
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bioRxiv - Bioengineering 2024Quote: ... The cell suspension was passed through a 70 μM cell strainer (FisherbrandTM; Thermo Fisher Scientific) and cells were centrifuged for 5 min at 1500 rpm and resuspended in 5 mL of ACK lysis buffer ...
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bioRxiv - Bioengineering 2024Quote: HEK293T cell were obtained from the cell bank(CAS) and cultured in 90% DMEM(Gibco), supplemented with 10% Fetal Bovine Serum(Gibco ...
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bioRxiv - Microbiology 2024Quote: ... Sequence verified maxipreps were used for transfections in HEK293T cells (ATCC) or Expi293F Cells (Thermofisher). Expi293F suspension cells were maintained at 125 rpm at 37°C with 8% CO2 in FreeStyleTM293 expression medium (Gibco) ...
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bioRxiv - Biophysics 2024Quote: U2OS FlipIn Trex cells and HeLa Kyoto cells (RRID:CVCL_1922) were cultured in DMEM-Glutamax (Gibco) supplemented with 10 % fetal bovine serum (FBS ...
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bioRxiv - Immunology 2024Quote: ... Cells were stained with LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Life Technologies L34965) and subsequently blocked with Purified Rat Anti-Mouse CD16/CD32 (BD Biosciences 553142) ...
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bioRxiv - Molecular Biology 2024Quote: ... Visualization of apoptotic cells was performed using the Evos FL Cell Imaging System (Life Technologies).
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bioRxiv - Biochemistry 2024Quote: ... The cell lines were maintained by passaging the cells with trypsin-EDTA solution (Gibco, 25200056) after the density reached 75%-90% in a 60 mm dish (Corning ...
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bioRxiv - Systems Biology 2024Quote: ... THP-1 monocytes (1 × 106 cells/ml) were labeled with DiI cell staining solution (Invitrogen) before being perfused through the chambers for 30 min ...
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bioRxiv - Cell Biology 2024Quote: All HeLa cells and the U2OS cells were cultured in DMEM/GlutaMAX medium (Gibco, 31966). All HCT116 cells were cultured in McCoy’s 5A/GlutaMAX medium (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... we evaluated cell proliferation using the Click-iT EdU Cell Proliferation Assay Kit (Invitrogen, #C10337). This kit utilizes a thymidine analog ...
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bioRxiv - Genomics 2024Quote: Cells were collected as described above and counted (automated cell counter Countess 3, Thermo Scientific; Countess™ Cell Counting Chamber Slides ...
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bioRxiv - Developmental Biology 2024Quote: ... The cells were then lysed in 80 µl cold Cell Extraction Buffer (Life Technologies/Invitrogen) containing cOmplete Protease Inhibitor Cocktail (Roche) ...
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bioRxiv - Systems Biology 2024Quote: ... Mount Sinai) cells were maintained in cell growth media: Dulbecco’s modified eagle medium (DMEM, Gibco) supplemented with 10 % heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell lysis was performed by scraping cells in 1 volume of TRIzol reagent (Ambion; #15596018), and incubating 5min on ice ...
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bioRxiv - Developmental Biology 2024Quote: ... The cells were then lysed in 80 µl cold Cell Extraction Buffer (Life Technologies/Invitrogen) containing cOmplete Protease Inhibitor Cocktail (Roche) ...
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bioRxiv - Bioengineering 2024Quote: Cells are stained with the Live/Dead mammalian cell viability kit (Invitrogen, Waltham, MA USA) as well as NucBlue nuclear probes (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: Live-dead cell analysis was conducted using the LIVE/DEAD Cell Imaging Kit (Thermo Fisher). For Calcein AM and Hoechst staining ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were counted and seeded (1×105 cells/well) onto poly-D-lysine (ThermoFisher Scientific)-coated plates (Agilent ...
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bioRxiv - Immunology 2024Quote: ... OTII cells were labelled with CellTrace™ Violet (CellTrace™ Violet Cell Proliferation Kit, Invitrogen) according to manufacturer’s recommendations.
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bioRxiv - Molecular Biology 2024Quote: ... cells were dissociated into single cells with TrypLE Select 1X (ref. A1217702, Thermo Fisher Scientific) and seeded at 200-300,000 cells/24 well on Geltrex-coated 24-well plates in StemFlex defined medium + 10µM ROCKi Y-27632 for 24h ...
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bioRxiv - Neuroscience 2024Quote: ... Live cells were imaged with an EVOS cell imaging system (Cat# AMF4300; Thermo Fisher Scientific). Images were processed in IMARIS (Oxford Instruments ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were assessed for ≥95% cell viability by Trypan blue exclusion assay (Gibco, 15250061). The cell-conditioned media was then used for the purification of EVs.
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bioRxiv - Neuroscience 2024Quote: ... The coverslip was mounted in a metal chamber (for HEK293T cells; Attofluor Cell Chamber, Invitrogen) or a magnetic chamber (for neurons ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell fractionation was performed using the subcellular protein fractionation kit for cultured cells (ThermoFisher 78840), following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2024Quote: ... cells were attached to concanavalin A-coated coverslips in Attofluor™ cell chambers (Invitrogen, USA) and imaged at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... cells were thawed and stained with LIVE/DEAD Fixable Aqua Dead Cell Stain (Life Technologies), as previously described90,91 ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were then replated at 200,000 cells/cm2 using StemFlex Medium (Thermo Fisher Scientific) and ROCK inhibitor (10μM ...
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bioRxiv - Microbiology 2024Quote: ... intracellular viral RNA was isolated by incubating cells with cell-to-cDNA lysis buffer (Ambion) at room temperature for 15 min and at 75°C for another 15 min ...
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bioRxiv - Neuroscience 2024Quote: ... Cells from the mouse embryonal carcinoma cell line 293T were grown in DMEM (Gibco, 12571063) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2024Quote: ... for HER2+ K562 cells or 5 nM Sytox red dead cell stain (Thermo Fisher Scientific) for SK-BR-3 cells to measure cytotoxicity ...
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bioRxiv - Systems Biology 2024Quote: Cells were routinely passaged twice a week as single cells using TrypLE select (Life Technologies) and replated in TESR medium that was further supplemented with the Rho kinase inhibitor Y27632 (Tocris ...
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bioRxiv - Cancer Biology 2024Quote: ... T-cells were then incubated with 1µL of CellTrace FarRed Cell Proliferation dye (ThermoFisher, C34564) per 1×106 cells (20 min ...
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bioRxiv - Cancer Biology 2024Quote: Cells in culture were detached from cell culture plasticware using StemPro accutase (Gibco, Gaithersburg, MD), washed with PBS and incubated with 1% BSA in 1x PBS for 10 min at 4°C ...
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bioRxiv - Physiology 2024Quote: ... cells were filtered with 100 µm mesh cell strainer (Cat #08-771-19, Fisher Scientific), and the hepatocytes were purified by 40% Percoll (Cat # P1644 ...
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bioRxiv - Physiology 2024Quote: ... Cell concentration was measured using an automated cell counter Countess 3 (ThermoFisher Scientific, cat#AMQAX2000).