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Citations for Thermo Fisher :
9501 - 9550 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... T-cells were then incubated with 1µL of CellTrace FarRed Cell Proliferation dye (ThermoFisher, C34564) per 1×106 cells (20 min ...
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bioRxiv - Cancer Biology 2024Quote: Cells in culture were detached from cell culture plasticware using StemPro accutase (Gibco, Gaithersburg, MD), washed with PBS and incubated with 1% BSA in 1x PBS for 10 min at 4°C ...
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bioRxiv - Neuroscience 2024Quote: SH-SY5Y cells were plated (100,000 cells/well) on 6-well plates (ThermoFisher Scientific, 353046) and allowed to adhere for 24 h ...
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bioRxiv - Cancer Biology 2024Quote: ... Data were normalized by cell number using the CyQUANT Cell Proliferation Kit (Thermo Fisher Scientific). For all measurements ...
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bioRxiv - Bioengineering 2024Quote: ... The cell suspension was passed through a 70 μM cell strainer (FisherbrandTM; Thermo Fisher Scientific) and cells were centrifuged for 5 min at 1500 rpm and resuspended in 5 mL of ACK lysis buffer ...
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bioRxiv - Bioengineering 2024Quote: HEK293T cell were obtained from the cell bank(CAS) and cultured in 90% DMEM(Gibco), supplemented with 10% Fetal Bovine Serum(Gibco ...
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bioRxiv - Microbiology 2024Quote: ... Sequence verified maxipreps were used for transfections in HEK293T cells (ATCC) or Expi293F Cells (Thermofisher). Expi293F suspension cells were maintained at 125 rpm at 37°C with 8% CO2 in FreeStyleTM293 expression medium (Gibco) ...
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bioRxiv - Biophysics 2024Quote: U2OS FlipIn Trex cells and HeLa Kyoto cells (RRID:CVCL_1922) were cultured in DMEM-Glutamax (Gibco) supplemented with 10 % fetal bovine serum (FBS ...
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bioRxiv - Immunology 2024Quote: ... Cells were stained with LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Life Technologies L34965) and subsequently blocked with Purified Rat Anti-Mouse CD16/CD32 (BD Biosciences 553142) ...
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bioRxiv - Molecular Biology 2024Quote: ... Visualization of apoptotic cells was performed using the Evos FL Cell Imaging System (Life Technologies).
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bioRxiv - Biochemistry 2024Quote: ... The cell lines were maintained by passaging the cells with trypsin-EDTA solution (Gibco, 25200056) after the density reached 75%-90% in a 60 mm dish (Corning ...
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bioRxiv - Systems Biology 2024Quote: ... THP-1 monocytes (1 × 106 cells/ml) were labeled with DiI cell staining solution (Invitrogen) before being perfused through the chambers for 30 min ...
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bioRxiv - Cell Biology 2024Quote: All HeLa cells and the U2OS cells were cultured in DMEM/GlutaMAX medium (Gibco, 31966). All HCT116 cells were cultured in McCoy’s 5A/GlutaMAX medium (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... we evaluated cell proliferation using the Click-iT EdU Cell Proliferation Assay Kit (Invitrogen, #C10337). This kit utilizes a thymidine analog ...
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bioRxiv - Genomics 2024Quote: Cells were collected as described above and counted (automated cell counter Countess 3, Thermo Scientific; Countess™ Cell Counting Chamber Slides ...
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bioRxiv - Developmental Biology 2024Quote: ... The cells were then lysed in 80 µl cold Cell Extraction Buffer (Life Technologies/Invitrogen) containing cOmplete Protease Inhibitor Cocktail (Roche) ...
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bioRxiv - Systems Biology 2024Quote: ... Mount Sinai) cells were maintained in cell growth media: Dulbecco’s modified eagle medium (DMEM, Gibco) supplemented with 10 % heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell lysis was performed by scraping cells in 1 volume of TRIzol reagent (Ambion; #15596018), and incubating 5min on ice ...
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bioRxiv - Immunology 2024Quote: ... OTII cells were labelled with CellTrace™ Violet (CellTrace™ Violet Cell Proliferation Kit, Invitrogen) according to manufacturer’s recommendations.
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bioRxiv - Cell Biology 2021Quote: ... and human SK-MEL-2 (hCLTAEN-Tq2 hDNM2EN-eGFP, as previously described(Scott et al., 2018)) were maintained in DMEM (Gibco, 4.5 g/L glucose, 110 mg/L pyruvate), supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: Microarray transcriptomic data from UII-hFGFR3-S249C and BBN mice was combined with transcriptomic array data from human bladder tumors (CIT; Affymetrix Exon 1.0 ST; 96 MIBC and 99 NMIBC). Batch effects due to data combination were corrected using the surrogate variable analysis R package ...
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bioRxiv - Immunology 2022Quote: ... Seahorse 96-well cell culture plates were pretreated with 1 µg/cm2 Corning™ Cell-Tak Cell and Tissue Adhesive (Fisher Scientific, Cat No CB40240) and FACS sorted Ly6Clow or Ly6Chigh monocytes were seeded at 100,000 cells/well ...
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bioRxiv - Cancer Biology 2021Quote: ... Control virus-infected and uninfected cells were treated with DMSO and their cell viability was measured with alamarBlue™ HS Cell Viability Reagent (Thermo Fisher Cat. No A50100). Cell death measured ...
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bioRxiv - Immunology 2021Quote: ... In order to assess proliferation T cells were resuspended at 1 × 106 cells/ml using a 2 μM solution of CellTrace™ Violet Cell Proliferation Kit (Thermo Fisher Scientific, Massachusetts, USA) in PBS ...
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bioRxiv - Bioengineering 2024Quote: ... Cell pellets were resuspended in 2 mL of cell maintenance media and quantified using a Countess automated cell counter (Thermo Fisher Scientific, Waltham, MA, USA). Seeding density for cell maintenance was approximately 9,300 cells/cm2 ...
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bioRxiv - Genomics 2024Quote: Cells were collected as described above and counted (automated cell counter Countess 3, Thermo Scientific; Countess™ Cell Counting Chamber Slides, Thermo Scientific, Cat. #C10283). For each reaction ...
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bioRxiv - Biochemistry 2021Quote: ... Before cell sorting the cells were filtered through a tubes with cell strainer (Falcon® Round-Bottom Tubes with Cell Strainer Cap, Thermo Fisher Scientific, Waltham, MA, USA) to remove cell aggregates ...
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bioRxiv - Cell Biology 2021Quote: ... Cell suspensions were filtered using a 70 μm nylon cell strainer and red blood cells were removed by 1×Red Blood Cell Lysis Solution (Thermo Fisher, Cat. no. 00-4333-57). Dissociated cells were washed with 1 × DPBS containing 2% FBS ...
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bioRxiv - Neuroscience 2022Quote: ... three times and incubated with horseradish peroxidase-conjugated secondary antibodies (Mouse, Cell Signaling, cat #7076S; Rabbit, Cell Signaling, cat #7074S; Rat, Cell Signaling, cat #7077S; Sheep, Thermo Fisher, cat #A16041) for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... The proportion of viable cells calculated by normalizing the viable cell number to the total number of cell nuclei based on Hoechst staining (Thermo Scientific, Hoechst 33342 Solution (20 mM)) ...
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bioRxiv - Microbiology 2020Quote: ... Dead cells were excluded using LIVE/DEAD ™ Fixable Blue Dead Cell Stain Kit (Life Technologies). Cells were fixed with 2% paraformaldehyde after staining ...
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bioRxiv - Cancer Biology 2021Quote: ... S18-shRictor and S18-shmTOR cells stained with CellTrace CFSE Cell Proliferation Kit (Invitrogen Cat#C34554) and cultured for 5 days ...
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bioRxiv - Cancer Biology 2021Quote: ... cell viability was measured with Calcein AM live cell staining dye (4 mM stock from Invitrogen), which stains viable cells with green fluorescence ...
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bioRxiv - Immunology 2021Quote: ... eWAT and liver cell suspensions were stained using a Fixable Aqua Dead Cell Stain Kit (Invitrogen) or Zombie UV Fixable Viability Kit (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... cells were first incubated with LIVE/DEAD Fixable Aqua Dead Cell Staining Kit (Invitrogen, Carlsbad, CA) for 20 minutes at 4 °C followed by two washes with FACS buffer (1X PBS+ 2% FBS) ...
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bioRxiv - Immunology 2021Quote: ... dead cells were excluded using LIVE/DEAD Fixable Aqua Dead Cell Staining Kit (Invitrogen, Carlsbad, CA).
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bioRxiv - Immunology 2021Quote: Cell Proliferation Dye staining was performed using the CellTrace Violet™ Cell Proliferation Kit by ThermoFisher scientific according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... plasmids were extracted from the yeast cells and transformed into electrocompetent Escherichia coli Stbl4 cells (Invitrogen). Plasmids were extracted using Qiagen miniprep kits and plasmid DNA was quantified using a Nan-oDrop spectrophotometer (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2020Quote: 293 cells or HeLa cells were cultured in Dulbecco’s-modified Eagle’s medium (Invitrogen, cat. no. C11995500BT) added with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated for 5h in RPMI complete medium containing eBioscience™ Cell Stimulation Cocktail (Invitrogen). Cells were then washed and fixed and permeablized with BD Cytofix/Cytoperm™ (BD ...
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bioRxiv - Cell Biology 2022Quote: ... cells were prepared using the Subcellular Protein Fractionation Kit for Cultured Cells (78840 from ThermoFisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were manually triturated in cell medium (DMEM + GlutaMAX with 5% horse serum, Thermo fisher, 26050-070 and 0.5% Penicillin-Streptomycin ...
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bioRxiv - Molecular Biology 2020Quote: ... Live/dead cell viability was assessed using Countess II FL Automated Cell Counters (Thermo Fisher Scientific). For each condition ...
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bioRxiv - Bioengineering 2022Quote: ... HEK293T cells and LHL2/3 cells were maintained in DMEM (Gibco, Grand Island, NY, United States) medium supplied with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: ... Confirmation of cell viability was performed using LIVE/DEAD™ Fixable Violet Dead Cell Stain (ThermoFisher), excited with the 405 laser and emission detected by via a 450/50 band pass filter.
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with LIVE/DEAD™ fixable near IR cell stain (Thermo Fisher Scientific) and incubated with Fc receptor blocking solution (Human TruStain FcXTM ...
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bioRxiv - Genomics 2020Quote: ... Cells were counted and checked for viability using a Countess II Automated Cell Counter (Thermo Fisher). Sequencing libraries were prepared as recommended in the user manual with cell target numbers ranging between 6,000 – 22,000 cells per sample ...
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bioRxiv - Genomics 2019Quote: ... Cell concentration and viability was determined with a Countess® II automated cell counter (Life Technologies).
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bioRxiv - Cancer Biology 2019Quote: ... A single cell suspension was obtained by incubation in Enzyme Free Cell Dissociation Buffer (Life Technologies) followed by mechanical trituration ...
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bioRxiv - Cell Biology 2019Quote: Cells were counted with a Countess™ II Automated Cell Counter (Thermo Fisher Scientific, Waltham, MA) and plated at a density of 2,500-3,500 cells/well ~24 hrs before imaging in minimal media in glass bottom 96-well plates (P96-1.5H-N ...