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Citations for Thermo Fisher :
9651 - 9700 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The cells were assessed for ≥95% cell viability by Trypan blue exclusion assay (Gibco, 15250061). The cell-conditioned media was then used for the purification of EVs.
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bioRxiv - Microbiology 2024Quote: ... intracellular viral RNA was isolated by incubating cells with cell-to-cDNA lysis buffer (Ambion) at room temperature for 15 min and at 75°C for another 15 min ...
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bioRxiv - Neuroscience 2024Quote: ... Cells from the mouse embryonal carcinoma cell line 293T were grown in DMEM (Gibco, 12571063) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were then replated at 200,000 cells/cm2 using StemFlex Medium (Thermo Fisher Scientific) and ROCK inhibitor (10μM ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were then replated at 200,000 cells/cm2 using StemFlex Medium (Thermo Fisher Scientific) and ROCK inhibitor (10μM ...
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bioRxiv - Cancer Biology 2024Quote: ... For CellTiter-Blue assay the cells were treated with 20x cell titer blue (Thermofisher #R12204) and cell viability was measured with EnVision multi-label plate reader (PerkinElmer) ...
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bioRxiv - Cancer Biology 2024Quote: Adherent cells were detached using enzyme free cell dissociation buffer (Thermo Fisher Scientific, Waltham, MA). Nectin-4 surface expression was evaluated by direct staining of 1×106 cells in 100 μL FACS buffer (PBS with 0.1% FBS and 2 mM ethylenediaminetetraacetic acid ...
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bioRxiv - Neuroscience 2024Quote: ... iPSCs were dissociated into single cells with StemPro™ Accutase™ Cell Dissociation Reagent (ThermoFisher) and seeded at a density of 2,500,000 cells per well on Matrigel-coated (human embryonic stem cell-qualified matrix ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell concentration and viability were determined using a Countess II Automated Cell Counter (ThermoFisher Scientific). 10X Genomics Chromium Single Cell 3’ Reagent Kit v3 library preparation and sequencing on Illumina HiSeq were performed by the YCGA.
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bioRxiv - Cancer Biology 2024Quote: ... cells were seeded at 2.7×104 cells/cm2 in William’s E medium (Gibco, 22511-022) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Bioengineering 2024Quote: ... Single cells were then stained with cell phenotyping antibodies (Table S2) in HBSS solution (ThermoFisher) containing 2% FBS ...
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bioRxiv - Biophysics 2024Quote: Cell counting was completed using the trypan-blue exclusion assay (Countess Automated Cell Counter; Invitrogen) to determine the concentration of cells in PBS ...
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bioRxiv - Developmental Biology 2024Quote: ... single cell suspensions were stained with Trypan blue and Countess II Automated Cell Counter (ThermoFisher) was used to assess both cell number and viability ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were passaged by washing cells with PBS (GIBCO/Thermo Fisher Scientific; Cat. No. 10010023), dissociating with StemPro Accutase Cell Dissociation Reagent (GIBCO/Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: Cell proliferation was quantitated using the Click-IT EdU cell proliferation assay (Invitrogen, Waltham, MA). Cells were incubated with Click-IT EdU dye for 4 hours and samples were acquired on Quanteon flow cytometer.
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bioRxiv - Biophysics 2024Quote: ... Total cell viability was measured using automated cell counting machine (Countess II FL, Life Technologies).
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RNA Binding of GAPDH Controls Transcript Stability and Protein Translation in Acute Myeloid LeukemiabioRxiv - Cancer Biology 2024Quote: Cells were washed with PBS prior to cell lysis with RIPA buffer (Thermo Fisher Scientific). The protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Cell counting and viability assessment were performed using an automated cell counter (Countess 3, Invitrogen) and 0.4% Trypan blue cell dye.
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bioRxiv - Bioengineering 2024Quote: ... Stained cells were imaged using a fluorescent microscope (EVOS FL Cell Imaging System, Thermo Fisher). Live and dead cell counts and percent viability were determined from the fluorescent images for each day using thresholding and particle analysis in ImageJ (at least n=5 frames captured at 10x magnification for each day).
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was assessed with Trypan Blue on an automated cell counter (Countess II, ThermoFisher), and up to 20,000 cells (∼5,000 cells from each hashtagged sample ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were stained with Hoechst 33342 (1 µg/mL, nuclear live cell stain, Life Technologies) and Sytox Orange (1 µM ...
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bioRxiv - Cell Biology 2024Quote: ... the number and viability of the resting cells was determined (Countess 3 Cell Counter (ThermoFisher)).
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bioRxiv - Cell Biology 2024Quote: ... ODs were corrected by the total number of cells counted (Countess 3 Cell Counter, ThermoFisher) upon exposure to each one of the conditions ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were first stained with Live/Dead Fixable Blue Dead Cell Stain Kit (ThermoFisher Scientific) for 30 minutes ...
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bioRxiv - Cancer Biology 2024Quote: Dissociated tumour cells were stained with Trypan blue and Countess II Automated Cell Counter (ThermoFisher) was used to assess both cell number and viability ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell suspension was filtered again through a 40 μm nylon cell strainer (Fisher Scientific, 087711) and 5 ml of PF10 were added to rinse it ...
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bioRxiv - Evolutionary Biology 2024Quote: ... cells were stained with 250 μM Cell Tracker Blue CMAC dye (Life Technologies, Carlsbad, CA) and 10 μM trypan blue (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... and resuspended in PBS for cell counting using an automated cell counter (Countess, Thermo Fisher). Then the doubling was calculated according to ...
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bioRxiv - Cell Biology 2024Quote: ... Cells from single cell-derived colonies were lysed in RIPA lysis buffer (Thermofisher Scientific, 89900) containing an EDTA-free protease inhibitor mini tablet (Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... K562 cell aggregates were then imaged on EVOS FLoid cell culture microscope (#4471136 Thermo Fisher).
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bioRxiv - Developmental Biology 2024Quote: ... Cells were passaged every other day as follows: cells were washed with PBS (Gibco, 10010023) and dissociated using Trypsin (Sigma T3924 ...
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bioRxiv - Cell Biology 2021Quote: ... and human SK-MEL-2 (hCLTAEN-Tq2 hDNM2EN-eGFP, as previously described(Scott et al., 2018)) were maintained in DMEM (Gibco, 4.5 g/L glucose, 110 mg/L pyruvate), supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: Microarray transcriptomic data from UII-hFGFR3-S249C and BBN mice was combined with transcriptomic array data from human bladder tumors (CIT; Affymetrix Exon 1.0 ST; 96 MIBC and 99 NMIBC). Batch effects due to data combination were corrected using the surrogate variable analysis R package ...
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bioRxiv - Immunology 2022Quote: ... Seahorse 96-well cell culture plates were pretreated with 1 µg/cm2 Corning™ Cell-Tak Cell and Tissue Adhesive (Fisher Scientific, Cat No CB40240) and FACS sorted Ly6Clow or Ly6Chigh monocytes were seeded at 100,000 cells/well ...
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bioRxiv - Cancer Biology 2021Quote: ... Control virus-infected and uninfected cells were treated with DMSO and their cell viability was measured with alamarBlue™ HS Cell Viability Reagent (Thermo Fisher Cat. No A50100). Cell death measured ...
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bioRxiv - Immunology 2021Quote: ... In order to assess proliferation T cells were resuspended at 1 × 106 cells/ml using a 2 μM solution of CellTrace™ Violet Cell Proliferation Kit (Thermo Fisher Scientific, Massachusetts, USA) in PBS ...
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bioRxiv - Bioengineering 2024Quote: ... Cell pellets were resuspended in 2 mL of cell maintenance media and quantified using a Countess automated cell counter (Thermo Fisher Scientific, Waltham, MA, USA). Seeding density for cell maintenance was approximately 9,300 cells/cm2 ...
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bioRxiv - Genomics 2024Quote: Cells were collected as described above and counted (automated cell counter Countess 3, Thermo Scientific; Countess™ Cell Counting Chamber Slides, Thermo Scientific, Cat. #C10283). For each reaction ...
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bioRxiv - Biochemistry 2021Quote: ... Before cell sorting the cells were filtered through a tubes with cell strainer (Falcon® Round-Bottom Tubes with Cell Strainer Cap, Thermo Fisher Scientific, Waltham, MA, USA) to remove cell aggregates ...
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bioRxiv - Cell Biology 2021Quote: ... Cell suspensions were filtered using a 70 μm nylon cell strainer and red blood cells were removed by 1×Red Blood Cell Lysis Solution (Thermo Fisher, Cat. no. 00-4333-57). Dissociated cells were washed with 1 × DPBS containing 2% FBS ...
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bioRxiv - Neuroscience 2022Quote: ... three times and incubated with horseradish peroxidase-conjugated secondary antibodies (Mouse, Cell Signaling, cat #7076S; Rabbit, Cell Signaling, cat #7074S; Rat, Cell Signaling, cat #7077S; Sheep, Thermo Fisher, cat #A16041) for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... The proportion of viable cells calculated by normalizing the viable cell number to the total number of cell nuclei based on Hoechst staining (Thermo Scientific, Hoechst 33342 Solution (20 mM)) ...
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bioRxiv - Microbiology 2020Quote: ... Dead cells were excluded using LIVE/DEAD ™ Fixable Blue Dead Cell Stain Kit (Life Technologies). Cells were fixed with 2% paraformaldehyde after staining ...
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bioRxiv - Cancer Biology 2021Quote: ... S18-shRictor and S18-shmTOR cells stained with CellTrace CFSE Cell Proliferation Kit (Invitrogen Cat#C34554) and cultured for 5 days ...
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bioRxiv - Cancer Biology 2021Quote: ... cell viability was measured with Calcein AM live cell staining dye (4 mM stock from Invitrogen), which stains viable cells with green fluorescence ...
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bioRxiv - Immunology 2021Quote: ... eWAT and liver cell suspensions were stained using a Fixable Aqua Dead Cell Stain Kit (Invitrogen) or Zombie UV Fixable Viability Kit (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... cells were first incubated with LIVE/DEAD Fixable Aqua Dead Cell Staining Kit (Invitrogen, Carlsbad, CA) for 20 minutes at 4 °C followed by two washes with FACS buffer (1X PBS+ 2% FBS) ...
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bioRxiv - Immunology 2021Quote: ... dead cells were excluded using LIVE/DEAD Fixable Aqua Dead Cell Staining Kit (Invitrogen, Carlsbad, CA).
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bioRxiv - Immunology 2021Quote: Cell Proliferation Dye staining was performed using the CellTrace Violet™ Cell Proliferation Kit by ThermoFisher scientific according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: 293 cells or HeLa cells were cultured in Dulbecco’s-modified Eagle’s medium (Invitrogen, cat. no. C11995500BT) added with 10% fetal bovine serum (FBS ...