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Citations for Thermo Fisher :
9551 - 9600 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Cells were grown on 100mm polystyrene cell culture dishes (Gibco, Thermo Fisher Scientific, Waltham, MA) for maintenance and on 35mm glass-bottom dishes - No ...
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bioRxiv - Biophysics 2023Quote: ... Cells were grown on 100mm polystyrene cell culture dishes (Gibco, Thermo Fisher Scientific, Waltham, MA) for maintenance and on 35mm glass-bottom dishes - No ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were washed and counted using an automated cell counter (Countess II, ThermoFisher). A range of 1 ...
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bioRxiv - Immunology 2023Quote: ... All single cell suspensions were counted in an automatic cell counter Countess 3 FL (Invitrogen) and prepared for subsequent staining and analysis as detailed below.
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bioRxiv - Immunology 2023Quote: ... red blood cells were removed using Red Blood Cell Lysis Buffer (Thermo Fisher Scientific, A1049201).
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bioRxiv - Cell Biology 2023Quote: ... Quantification of cell viability was performed with an automated cell counter (Invitrogen Countess 3 FL). Cell suspensions with < 85% viability were not used for downstream flow cytometry assays.
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bioRxiv - Cancer Biology 2023Quote: ... Cells were stained with Live/Dead fixable nearIR or blue dead cell staining kit (ThermoFisher). Samples were then fixed and permeabilized with eBioscience Foxp3/Transcription Factor Staining Buffer Set (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... A549 cells and HEK293T cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, 11965118), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Bioengineering 2023Quote: ... after 48h stimulation of the cells with PMA/Ionomycin (Cell stimulation Cocktail, Thermo Fisher Scientific). Binding of T cell-secreted SiRPα-Fc on T cell CD47 was assessed by staining with labeled goat anti-human IgG Fc (HP6017 ...
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bioRxiv - Bioengineering 2023Quote: ... T cells were stimulated for 48h with PMA/Ionomycin (Cell stimulation Cocktail, Thermo Fisher Scientific) or PHA (Sigma).
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bioRxiv - Cancer Biology 2023Quote: Tumor cells were stained with 10 µM CellTrace CFSE Cell Proliferation Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were counted with 1:1 ratio of cell solution: Trypan Blue (Life Technologies, NY) using an automated cell counter (Countess II FL ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were also split as single cells using accutase (Life Technologies, Waltham, MA, USA) while seeding for organoid generation ...
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bioRxiv - Genetics 2023Quote: ... the cell count was measured using the PrestoBlue™ Cell Viability Reagent (Invitrogen™ #A13261) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: Sorted cells were counted and checked viability on a Countess 3 Automated Cell Counter (ThermoFisher) with Trypan blue (0.4% ...
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bioRxiv - Immunology 2023Quote: ... Dead cells were excluded using LIVE/DEAD Fixable Aqua Dead Cell Stain (ThermoFisher cat#: L34957). The antibodies used are the following ...
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bioRxiv - Immunology 2023Quote: ... OT1 or P14 cells are stained with 2μM Cell Trace Far Red (Invitrogen, Cat#C34572) at 106 cells/mL for 15 minutes at 37°C and rested 15 minutes at 37°C ...
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bioRxiv - Immunology 2023Quote: ... The cell viability was assessed using Trypan blue and a Countess automated cell counter (Invitrogen). A 96 well plate was pre-incubated with a 5 µg/ml solution of anti-mouse CD3e (100314 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were kept on ice and incubated with SYTOX Blue Dead Cell Stain (ThermoFisher, S34857) prior to FACS analysis ...
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bioRxiv - Systems Biology 2023Quote: ... live cell size and dead cell size data were collected through reading Trypan Blue (Gibco) stained cells via Countess II FL (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: Whole-cell lysates were prepared using cell extraction lysis buffer (ThermoFisher, Waltham, MA. Catalog: FNN0011) and sonication ...
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bioRxiv - Bioengineering 2022Quote: ... The cell culture dishes for adherent cells (treated surface) were procured from Nunc (Thermo Scientific). Dulbecco’s modified Eagle’s medium (DMEM) ...
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bioRxiv - Bioengineering 2023Quote: COS-7 cells and U2OS cells were cultured high glucose medium DMEM (Gibco, 11995-040) with the addition of 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was isolated from cell lines or acinar cells with Trizol® reagent (ThermoFisher) according to supplier’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: The T-cell engager (UCHT-2-03) was cloned and expressed using ExpiCHO cells (Invitrogen), and the recombinant protein was purified using Ni-column and size-exclusion chromatography (SEC).
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bioRxiv - Biophysics 2023Quote: ... cells were harvested and whole cell lysates were prepared using IP lysis buffer (Thermo Scientific) supplemented with protease inhibitor cocktail (Roche) ...
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bioRxiv - Biophysics 2022Quote: ... Cells were finally stained firstly with 1 LIVE/DEAD fixable aqua dead cell stain (ThermoFisher) in the same buffer for another 15 minutes and subsequently washed twice in plates with FACS buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were first stained with LIVE/DEAD Fixable Blue Dead Cell Stain Kit (ThermoFisher Scientific) according to manufacturer instructions ...
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bioRxiv - Genomics 2023Quote: ... EY.T434 mouse embryonic fibroblast cells mouse embryonic fibroblast cells were grown in DMEM (ThermoFisher 10566016) supplemented with 15% (vol/vol ...
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bioRxiv - Cell Biology 2023Quote: ... for 293T cells and at 50-60% cell density with Lipofectamine 3000 (ThermoFisher Scientific, L3000001) for SH-SY5Y cells ...
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bioRxiv - Immunology 2023Quote: T cells were cultured unstimulated or stimulated with cell stimulation cocktail (00-4970-03; Invitrogen) for 3 h at 37°C in 5% CO2 ...
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bioRxiv - Biochemistry 2023Quote: ... and a fraction of the cells were plated onto 35 mm cell culture dishes (Thermofisher). PANX1 and its mutants and PANX3 were transiently transfected into HEK293 cells with an enhanced green fluorescent protein (eGFP ...
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bioRxiv - Immunology 2023Quote: ... To single-cell suspensions was added a live/dead cell staining (Invitrogen, L10119; Waltham, MA) and the following antibodies:BV510 anti-CD45 (103137) ...
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bioRxiv - Genomics 2023Quote: ... Cells were analyzed using the Click-iT EdU Cell Proliferation Kit for Imaging (Invitrogen, C10337) according to the manufacturer’s instructions.
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bioRxiv - Genomics 2023Quote: ... After four days of treatment cells were dissociated into single cells with Accutase (Thermo Fisher) at 37°C for 8 -10 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... The GFP-reporting MDA-MB-231 cells and Cell Tracker Green CMFDA (Thermo Fisher Scientific)-stained KTB21 cells were then seeded on the Exo-Glow-stained tissue sections at 1 million cells/mL seeding density and incubated overnight in 96-well plates at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were stained with LIVE/DEADTM Fixable Near-IR Dead Cell Stain Kit (Invitrogen, USA) and acquired by flow cytometry ...
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bioRxiv - Neuroscience 2023Quote: ... Tumor cell concentrations and viability were determined using a Cell Countess II FL device (ThermoFisher) before calculating cell counts and loading the suspension into the Next GEM Chip G and Chromium Controller (10x Genomics ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA was extracted from PC cells and MIN6 cells with TRIzol reagent (Invitrogen, USA). The cDNA templates were synthesized with PrimeScript RT Reagent Kit (TaKaRa ...
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bioRxiv - Microbiology 2023Quote: ... Cell viability was determined using LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit (Invitrogen). Intracellular staining for transcription factors was performed using the eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... Cell number and viability were measured by CountessTM II Automated Cell Counter (Thermo Fisher®). Final cell density was adjusted to 1.0×106cells/ml with >95% viability ...
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bioRxiv - Cancer Biology 2023Quote: AML cells (6 x104 cells/cm2) were seeded for 24h on poly-D-Lysine (Gibco) coated Lab-Tek II Chamber Slides (Nunc ...
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bioRxiv - Cancer Biology 2023Quote: The M07e cell line was seeded at 1x106 cells/ml and cultured in RPMI (Gibco) +10%FBS +1X Glutamax (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... Sf9 insect cells were grown at 27°C in Grace’s Insect Cell Culture Medium (Gibco) supplemented with 4.175mM sodium bicarbonate ...
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bioRxiv - Developmental Biology 2023Quote: ... the hES cell colonies were dissociated into single cells using TrypLE Express (Gibco, 12604-013) and seeded on Matrigel coated plates as described above at a concentration of 95,000 cells/cm2 in mTeSR supplemented with 20 µM ROCKi ...
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bioRxiv - Cell Biology 2023Quote: ... cells were counter-stained with the live-cell DNA dye Hoechst 33342 (Invitrogen, cat. #H3570) by incubation for 30 minutes at 37°C in 150 µl of Hoechst diluted to 1µg/mL in culture media ...
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bioRxiv - Molecular Biology 2023Quote: ... MCF7 cells (750 cells/well) were reverse transfected using Lipofectamine RNAiMAX (0.15ul/well, Thermofisher 13778150) with a final siRNA concentration of 20nM ...
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bioRxiv - Molecular Biology 2023Quote: ... ES cells were cultured on mitotically arrested mouse embryonic fibroblast cells in DMEM (Life Technologies), supplemented with 15% v/v FBS (Bodinco BV) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were mixed with trypan blue and counted using an automated cell counter (Countess, Invitrogen). Cell doubling time was estimated to be 26.5 h by linear regression of the log2-fold change in cell number over time ...
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bioRxiv - Cancer Biology 2023Quote: Cell viability was examined by the LIVE/DEADTM Cell Viability Assay Kit (Molecular Probes, Inc.) 48 hr after gelation ...