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Citations for Thermo Fisher :
9951 - 10000 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... Blocking and permeabilization were performed for 1 h in 1 mL blocking buffer - Dulbecco’s phosphate buffered saline (DPBS) containing 5% (v/v) normal goat serum (Thermo Fisher Scientific) and 0.3% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... and followed the same procedure described above until the application of the secondary antibody solution (5% normal goat serum in PBST, Alexa Fluor 488 anti-guinea pig 1:200 (Thermo Scientific)) ...
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bioRxiv - Cell Biology 2023Quote: ... the transfected cells were blocked with 5% BSA at room temperature for 15 min and labeled with anti-FLAG antibody (1:100, Thermo Fisher) at 4°C for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... and ATTO647N (Atto-Tec) were dissolved in DMSO (10 and 5 mg.mL-1, respectively) while octadecyl rhodamine B chloride (R18, Thermo Fisher Scientific) was dissolved in ethanol (10 mM).
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed in PBS (2 x 5 min) and incubated in 0.1% Tween in PBS containing streptavidin Alexa 594 conjugate (ThermoFisher, 1:1500) for 2 hours at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... Chromatographic separation was carried out using a Hypercarb porous graphitic carbon (PGC) column (5 µm, 150 mm x 1 mm, Thermo Scientific). A 40-minute binary gradient using 3% ACN in water containing 0.1% formic acid (Solvent A ...
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bioRxiv - Neuroscience 2024Quote: ... and NOD2−/− primary microglia treated with M18-MDP (1 μg/ml) or α-Syn PFF (5 μg/ml) were lysed in ubiquitination lysis buffer (#PI89900, ThermoFisher), supplemented with protease inhibitor ...
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bioRxiv - Neuroscience 2024Quote: ... neurons were washed with PBS three times for 5 min and incubated with secondary antibodies (goat anti-rabbit-AlexaFluor488: 1:1000, Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... cells were cultured and transiently co-transfected with wild-type or mutant Nav1.2 channel variant (5 μg) and enhanced green fluorescent protein (1 μg) using Lipofectamine 3000 kit (Thermo Fisher Scientific), as previously described (26) ...
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bioRxiv - Bioengineering 2023Quote: ... The samples were then blocked with a 5% (v/v) FBS solution and incubated for 90 min in a 1:200 phalloidin–Alexa Fluor 594 (Life Technologies) diluted in a 1% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... the beads were collected by centrifugation and washed with inhibitor-containing lysis buffer 5 times before being mixed with 20-40 μl 1× Laemmli sample buffer (diluted from Invitrogen, NP0008) supplemented with β-mercaptoethanol ...
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bioRxiv - Microbiology 2023Quote: ... at 1:1000 in 5% BSA in TBST was used for viral loading control and α-GAPDH primary antibody (Invitrogen #AM4300) at 1:1000 in 5% BSA in TBST for cellular loading control ...
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bioRxiv - Genomics 2023Quote: ... for 5 min at room temperature and washed them three times with 1× PBS (diluted from 10× PBS Liquid Concentrate, Gibco, USA). We then lysed parasites with 0.1% Sarkosyl Solution (Bioworld ...
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bioRxiv - Genetics 2024Quote: ... Samples were resuspended in 50 ul lysis buffer (50 mM Na-HEPES pH 7.5, 200 mM NaOAc, 1 mM EDTA, 0.25% NP-40, 3mM DTT, and 1X Halt Protease Inhibitor Cocktail [Thermo Fisher Scientific]). Approximately 100 ul of 0.5 mm glass beads were added to each sample ...
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Leveraging single cell multiomic analyses to identify factors that drive human chondrocyte cell fatebioRxiv - Developmental Biology 2024Quote: ... and cultured in suspension in the presence of BMP4 (1 ng/mL) and Y-27632 (5 μM) in Stem-Pro-34 media (Life Technologies) supplemented with L-glutamine ...
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bioRxiv - Bioengineering 2024Quote: ... in 50 µL of PBS + 5% FBS for 30 minutes followed by a 1:1000 dilution of Alexa Fluor 488-conjugated secondary antibody (Invitrogen; A10631) in 50 µL of PBS + 5% FBS for another 30 minutes.
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bioRxiv - Developmental Biology 2024Quote: ... Enzymatic digestion was stopped by adding 500 μl of HBSS+ (HBSS, 5% Fetal Bovine Serum, 1% HEPES (Thermo Fisher, 15630-080) 1M ...
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bioRxiv - Microbiology 2024Quote: ... The dry pooled sample was resuspended in 1 ml of 5% formic acid and was desalted using the EasyPep Maxi Kit (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Brain sections were blocked and permeabilized for 1 hour in 1xPBS containing 5% normal goat serum (ref # 16210064, Thermo Fisher Scientific) and 0.5% Triton X-100 (ref # BP151 ...
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bioRxiv - Microbiology 2024Quote: ... The grids were immediately blotted for 1-5 s and plunge-frozen in liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific) device operating at 4 °C and 100% humidity ...
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bioRxiv - Molecular Biology 2024Quote: ... and the flowthrough was ethanol precipitated in the presence of 5 mM MgCl2 and 1 µL GlycoBlue (Invitrogen, Cat. No. AM9516). The RNA pellet was resuspended in 10 µL of RNase-free water and immediately processed for library preparation.
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bioRxiv - Biophysics 2024Quote: ... The resulting peptides are trapped and desalted on a C18 trapping column (Acclaim 5 µm, PepMap 300, 1 mm x 15 mm, Fisher Scientific). Once desalting is complete the trap column is placed in line with a separation column (Hypersil GOLD 1.9 µm particle size ...
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bioRxiv - Synthetic Biology 2024Quote: ... and afterwards stained for 1 h in 50 ml of 0.5X TBE buffer supplemented with 5 µl of SYBR Gold dye (Invitrogen, Waltham, MA) while gently shaken ...
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bioRxiv - Microbiology 2024Quote: ... THP-1 monocytes were cultured at 37°C with 5% CO2 to confluence in RPMI media (MEDIUM 1640 CE, Gibco, 21875034) supplemented with 10% FBS ...
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bioRxiv - Biophysics 2024Quote: ... wild-type α-synuclein was mixed with an A90C variant labelled with Alexa 647 at a 100:1 molar ratio in 50 mM Tris-HCL and 5% polyethylene glycol 10,000 (PEG) (Thermo Fisher Scientific). The final mixture was pipetted onto a 35-mm glass-bottom dish (P35G-1.5-20-C ...
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bioRxiv - Microbiology 2020Quote: ... and NanoDrop One (Thermo Scientific, Waltham, Massachusetts, USA). pH-CaCl2 and pH-H2O of ms soils was measured as described earlier (section 2.2.2 ...
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bioRxiv - Microbiology 2020Quote: ... coli DH5α and One Shot™ PIR2 (Thermofisher) were required for standard genetic manipulations and plasmid maintenance (Tables S1 and S2).
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bioRxiv - Cancer Biology 2021Quote: ... in a Step One Thermal Cycler (Applied Biosystems) essentially as indicated (Rico-Leo et al. ...
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bioRxiv - Microbiology 2021Quote: ... and electroporated into One Shot Top10 cells (Invitrogen). The plasmid containing the cloned chitinase gene was isolated using the Qiaprep spin miniprep kit (Qiagen) ...
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bioRxiv - Molecular Biology 2020Quote: ... on a Step One Plus (Applied Biosystems, USA) machine ...
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bioRxiv - Genomics 2020Quote: ... 1μl of RNase One (10U/μl) (Thermo Scientific), and 1μl of Benzonase Nuclease (250U/μl ...
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bioRxiv - Neuroscience 2019Quote: ... one drop of each ReadyProbes reagent (Life Technologies) was added to cells in 500 μL of media ...
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The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: ... one part of Ham’s F12 medium (Life Technologies), 10% fetal bovine serum (Hyclone) ...
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bioRxiv - Microbiology 2019Quote: ... 1μl of RNase One (10U/μl) (Thermo Scientific), and 1μl of Benzonase Nuclease (≥250U/μl ...
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bioRxiv - Developmental Biology 2020Quote: ... in a step one thermal cycler (Applied Biosystems) essentially as described (Rey-Barroso et al. ...
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bioRxiv - Biochemistry 2020Quote: ... A Step One Plus PCR system (Applied Biosystems) was used for incubation at 37 °C as well as for fluorescence measurements over 710 minutes.
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bioRxiv - Evolutionary Biology 2020Quote: ... A one-step hemi-nested RT-PCR (Invitrogen) was used to detect coronavirus RNA using a set of primers targeting a 440-nt fragment of the RdRp gene and optimized for bat-CoV detection (CoV-FWD3 ...
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bioRxiv - Biochemistry 2020Quote: One Shot™ Stbl3 (Fisher Scientific, Cat. # C737303)
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bioRxiv - Biochemistry 2020Quote: One Shot™ TOP10 (Fisher Scientific, Cat. # C404003)
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bioRxiv - Immunology 2020Quote: ... One µM Sytox blue or Sytox Green (ThermoFisher) was used to measure inner membrane damage ...
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bioRxiv - Microbiology 2019Quote: ... A spectrophotometer was used (Thermo Scientific NanoDrop One) to measure the concentration and purity of the DNA extracts ...
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bioRxiv - Microbiology 2020Quote: ... coli One-Shot TOP10 or DH5α strains (Thermofisher). H ...
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bioRxiv - Cell Biology 2021Quote: ... in One Shot Top10 chemically competent cells (Invitrogen). Proper insertion was confirmed by sequencing ...
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bioRxiv - Cancer Biology 2021Quote: ... One hundred µL of D-Luciferin (88292, Thermofisher, Waltham ...
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bioRxiv - Genetics 2022Quote: ... and a NanodropTM One (ThermoFisher, Waltham, MA, USA) to determine the quantity and quality of DNA released with FUSE and control samples ...
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bioRxiv - Neuroscience 2022Quote: ... 1X Culture One (all from Thermo Fisher Scientific), 40 ng/mL BDNF (PeproTech) ...
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bioRxiv - Genetics 2020Quote: ... on the Step One Plus (Thermo Fisher Scientific). Reactions were performed in technical duplicates ...
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bioRxiv - Microbiology 2021Quote: ... and Nanodrop One (Thermo Fisher Scientific, MA, USA). Quality of RNA samples was assessed by Agilent 2100 system (Agilent Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... SuperScript IV One-step RT-PCR kit (ThermoFisher) was used for detection of transcripts ...
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bioRxiv - Immunology 2020Quote: ... supplemented with One Shot dialyzed FBS (Thermo Fisher), 1mM sodium pyruvate ...