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Citations for Thermo Fisher :
9751 - 9800 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... cells at 70-80% confluency were switched to myogenic differentiation medium (DMEM, 4% horse serum, 2 mM Glutamax, 1 mM sodium pyruvate (Life Technologies #11360070), 1 μg/mL insulin (GeminiBio #800122 ...
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bioRxiv - Cell Biology 2024Quote: ... They were then incubated overnight at 4 °C with the primary antibody (anti-TMEM173/STING antibody (OT14H1),1:100 dilution) (Invitrogen, MA,USA). After they were rinsed with PBS-T ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were passaged with a ratio of 1:4 to when they reached 70% to 80% confluency by incubation cells with 0.5 mM EDTA (Fisher Scientific MT-46034CI) at room temperature for 5 minutes.
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bioRxiv - Cancer Biology 2022Quote: Approximately 5 × 106 MCF10AER/vSrc cells (Control and treated with 10 nM D-Alanine for 4 h) were fixed with 1% methanol-free formaldehyde solution (Thermo Scientific, #28908) for 10 min at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were pre-extracted with 0.1% Tween-20 in PBS (PBST) for 1 min and then fixed with 4% methanol-free formaldehyde (Thermo Fisher Scientific, 28906) for 20 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... hTERT immortalized BJ-5ta foreskin fibroblasts were cultured in a 4:1 mixture of DMEM medium and Medium 199 (Gibco, Life Technologies) supplemented with 10% FBS ...
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bioRxiv - Developmental Biology 2023Quote: ... the sections were incubated overnight at 4°C with the following primary antibodies diluted in blocking buffer: rat anti-SOX2 (1:200, 14-9811-82, Thermo Fisher Scientific), goat anti-T (1:200 ...
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bioRxiv - Biochemistry 2023Quote: ... then resuspended in 100 µL of secondary antibody (1:500 α-mouse IgG-Alexa647 [ThermoFisher, Waltham, MA] in TBST with 4% BSA), and incubated in the dark for 60 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... incubated at 65°C for 5 min and then immediately chilled on ice for at least 2 min before adding 4 μL reverse transcription reaction mix [in 10 μL RT reaction: 1× First Strand buffer (Invitrogen – 18064- 014), 20 mM DTT (Invitrogen - 18064-014) ...
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TNFR1/p38αMAPK signaling in Nex+ supraspinal neurons regulates sex-specific chronic neuropathic painbioRxiv - Neuroscience 2023Quote: ... for two hours at room temperature Sections were incubated in the following primary antibodies overnight at 4 °C: ERβ (rabbit, 1:200, Invitrogen PA1-311) and NeuN (mouse ...
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bioRxiv - Genomics 2023Quote: ... for 1 hour, followed by incubation with primary antibodies (overnight, 4°C) and secondary antibodies (1-2 hours, from Jackson ImmunoResearch or ThermoFisher Scientific). The slides were then mounted with coverslips using VECTASHIELD (Vector Labs ...
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bioRxiv - Immunology 2023Quote: ... CTLA-4 or PD-1) were suspended in IL-2 medium at 37°C containing either 65nM LysoTracker® Deep Red (ThermoFisher Scientific) or 25nM MitoTracker™ Red CMXRos for 1h or 30min ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then stained with primary antibodies overnight at 4°C targeting the following proteins: MAP2 (PA1-10005, ThermoFisher Scientific, 1:5000), AT8 (MN1020 ...
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bioRxiv - Cell Biology 2023Quote: ... Ex vivo lung slices were washed 3 × 30 minutes in PBS+0.5% Triton X-100) before incubating overnight at 4°C overnight with: 1:100 Alexa Fluor 488 goat anti-rabbit (Thermo Scientific - A11008) or anti-mouse (A32723 ...
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bioRxiv - Biochemistry 2023Quote: ... ATP measurements were taken before and after activation with magnetic Dynabeads coated with anti-CD3/CD28 antibodies (Thermofisher, 4:1 beads/cell).
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bioRxiv - Cell Biology 2023Quote: ... primary antibodies in 10% GS in 1xPBS overnight at 4°C followed by a 1h incubation with anti-mouse (A11003, Invitrogen, 1:800) secondary antibody in 10% GS in 1xPBS at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... mucus-like compounds were stained by incubation (overnight at 4°C) with AlexaFluor 647-labeled PNA (1:1000 in PBSt; Molecular Probes #L32460).
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bioRxiv - Immunology 2023Quote: ... a liver burn injury of approximately 1 mm3 was made on which a droplet of pHrodo Red succinimidyl ester (4 μM; Thermo Fisher Scientific) was administered ...
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bioRxiv - Immunology 2023Quote: ... The sections were fixed for 1 hour with 4% paraformaldehyde (PFA) in Hank’s Balanced Salt Solution (HBSS, Gibco, Waltham, MA, pH 7.2) supplemented with 0.1% Bovine Serum Albumin (BSA ...
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bioRxiv - Cell Biology 2023Quote: ... the coverslips were washed and fixed with 4% paraformaldehyde in PBS for 10 min at room temperature then stained with Alexa Fluor 488 Phalloidin (1:500; Invitrogen cat# A12379) in blocking buffer for 20 min at room temperature and mounted in DuoLink PLA mounting medium with DAPI ...
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bioRxiv - Molecular Biology 2023Quote: ... lysates were incubated for overnight at 4°C with 2 μg of antibodies recognizing MTR4 or IgG control and then incubated for 1 h at 4°C with Dynabeads™ Protein A (ThermoFisher Scientific). After incubation ...
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bioRxiv - Neuroscience 2023Quote: ... a series of every 6th section was immersed in 1% skim milk for 1 h at room temperature and incubated overnight at 4°C with rabbit anti-GFP monoclonal antibody (1:500, G10362, Thermo Fisher Scientific) i n PBS containing 0.1% Triton X-100 and 1% normal goat serum for 2 days at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were washed in PBS three times for 5 min at 4 °C by rocking and then incubated by rocking with secondary antibody (Goat-anti-Mouse 488, 1:2000, Invitrogen #A-11008) in 1% BSA PBST for 1 hour at RT in the dark ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were blocked with 5% non-fat dry milk overnight at 4°C under gentle agitation followed by 1,5 hours incubation at room temperature with anti-CRM1 (MA5-27879, Invitrogen, 1:2000 dilution) antibody to detect PmXPO1 protein ...
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bioRxiv - Cell Biology 2023Quote: ... 5 µl polar metabolite extract were injected in push partial mode using an overfill factor of 1 onto a Dionex IonPac AS11-HC column (2 mm × 250 mm, 4 μm particle size, Thermo Fisher Scientific), additionally this was equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm ...
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bioRxiv - Biochemistry 2023Quote: ... The grid was blotted for 3s (blot force 1) at 100 % humidity and 4°C in a Vitrobot Mark IV (Thermo Fisher Scientific) before plunge-freezing in liquid ethane.
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bioRxiv - Genomics 2023Quote: ... 5 ul of cDNA reaction was used in each of 4 x 50 ul Round 1 PCRs per sample using Phusion polymerase in HF buffer (Life Technologies-F531L). The 4 Round 1 PCRs were then pooled and 5 ul of that mix were used as template for a 50 ul Round 2 PCR ...
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bioRxiv - Cancer Biology 2024Quote: ... the following secondary antibody was added overnight at 4°C in blocking buffer: Alexa FluorTM 647 donkey anti-rabbit IgG (1:1000, Invitrogen #A-32795). Finally ...
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bioRxiv - Immunology 2024Quote: ... 67 or Bacillus anthracis were loaded to 1-well 4-12% Bis-Tris NuPAGE Novex SDS-PAGE gels (Invitrogen, Thermo Fisher Scientific). Proteins were separated for 45 min at 200 V ...
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Entorhinal cortex vulnerability to human APP expression promotes hyperexcitability and tau pathologybioRxiv - Neuroscience 2024Quote: To confirm protein enrichment on the pulldown samples the protein were eluted from the beads as mentioned above and 1/3rd of the eluted protein was resolved on a 4–12% Bris-Tris gel (Invitrogen: cat# NW04125box) and transferred onto transferred onto iBlot 2 Transfer Stack containing nitrocellulose membrane using the BOLT transfer system ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein samples were separated by SDS-PAGE ((4-20%; Criterion XT precast gel) with 1× MOPS SDS gel running buffer (NP0001, Invitrogen, Life Technologies Corp). Following electrophoresis ...
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bioRxiv - Cancer Biology 2024Quote: ... 200 ng of RNA were mixed with 4 µl of diluted ERCC RNA Spike-In Mix (Invitrogen, cat# 4456740. 1:1,000 dilution). All libraries were sequenced on the Illumina NovaSeq 6000 system.
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bioRxiv - Bioengineering 2024Quote: ... Then secondary antibodies were then added in blocking buffer over night at 4 ºC (Goat anti Rabbit 647, Thermo Fisher Scientific A21245, 1:1000; Rhodamine Phalloidin, Fisher Scientific 50-646-256 ...
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bioRxiv - Cancer Biology 2024Quote: ... The PVDF membrane was blocked for 1 h with 5% BSA in TBST buffer containing 0.1% Tween 20 followed by overnight incubation at 4 °C with primary antibody for: ERα (MA5-14104, dilution 1:400, Thermo Fisher Scientific) and PR (sc-7208 ...
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bioRxiv - Immunology 2024Quote: CD14-positive monocytes isolated from human PBMCs (1 x 106 cells/well) were incubated with 27D6 antibody or isotype-matched control antibody (4 μg/mL, IgM, Thermo Fisher Scientific) for 30 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Program T-023 with 150 pmol/106 cells of human TSP-1 and TSP-4-specific siRNAs (#s14108 and #s14100, Thermo Fisher Scientific) and control scrambled siRNAs (#4390846 ...
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Controlled Fluorescent Labelling of Metal Oxide Nanoparticles for Artefact-free Live Cell MicroscopybioRxiv - Biophysics 2021Quote: ... the medium with cells was removed and cells were labelled with 100 µL of 5 µg mL-1 CellMask Orange in Live Cell Imaging Solution (LCIS, Molecular Probes) for 6 minutes at 37 °C ...
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bioRxiv - Biophysics 2021Quote: 106 T-cells were incubated in T-cell media supplemented with 5 µg ml-1 Fura-2 AM (11524766, ThermoFisher Scientific) for 20 min at 24°C ...
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bioRxiv - Biophysics 2020Quote: The cells were pelleted by centrifugation 5 °C at 4000 rpm for 1 hour by a Sorvall Legend X1R Centrifuge (Thermo Fisher) and resuspended in 20 mL of 100 mM KH2PO4/K2HPO4 (Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... Pre-amplified product was diluted 1 to 5 and loaded onto a 48.48 chip together with Taqman universal MasterMix (Applied Biosystems 4304437) and the Taqman assays listed in (Supplementary Table 1 ...
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bioRxiv - Cell Biology 2020Quote: ... and then incubated overnight at 4°C in 2 ml TBST/5% milk with a 1:1,000 dilution of anti-CPY Monoclonal antibody 10A5B5 (Invitrogen, A-6428). The nitrocellulose was then washed 3 times in 20 ml TBST at room temperature for 10 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Twenty million cells were diluted in 2 mL of GBSS containing 1% FBS and stained with 5 μg/mL of Hoechst 33342 (Invitrogen H3570) for 1 h at 32 °C ...
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bioRxiv - Microbiology 2021Quote: ... The membrane was blocked with PBS + 0.1% Tween 20 (PBS-T) and 5% nonfat milk prior to incubating in monoclonal anti-Flag M2 antibody (1:10,000, Sigma, Thermo Scientific™) overnight at 4 °C followed by Goat-anti Mouse IgG-HRP secondary antibody (Invitrogen™ ...
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bioRxiv - Molecular Biology 2022Quote: ... Hemocytes were released from 5-10 thoroughly washed larvae into a sample chamber containing 1 mL filtered Schneider’s Drosophila medium (Gibco™ 21720024) including an EDTA-free protease inhibitor cocktail (Roche 4693159001) ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was diluted 1:5 in water and 2μl was used for real-time PCR using SYBR® Green PCR master mix (Applied Biosystems) and a Quant Studio 5 real-time PCR machine (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2021Quote: ... and the flow through was ethanol precipitated in the presence of 5 mM MgCl2 and 1 µL of Glycoblue (Invitrogen AM9516). The RNA pellet was resuspended in 10 µL of RNase-free water and immediately processed for library preparation.
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bioRxiv - Cell Biology 2022Quote: ... 30 to 50 μl of cell lysate was digested using a 5:1 mixture of nitric acid (OPTIMA grade, 70%, Fisher Scientific) and ultrapure hydrogen peroxide (ULTREX II ...
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bioRxiv - Genomics 2019Quote: ... we seeded 2×105 HEK293FT cells per well of a 24-well plate prior to transfection (12 - 18 hours) and used 500 or 750 ng plasmid together with a 5-to-1 ratio of Lipofectamine 2000 (ThermoFisher 11668019) or 1 mg/mL polyethylenimine (Polysciences 23966 ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 μL of magnetic HA beads was washed twice in 1X PBS with 0.1% Tween 20 and then twice in binding buffer (50 mM Tris pH7.5, 150 mM NaCl, 1% IGEPAL CA-630, 5% glycerol, and protease inhibitors (Thermo Fisher #A32955): apoptin ...
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bioRxiv - Systems Biology 2020Quote: ... of the immunodepleted pooled CSF samples were separately dissolved in 100 mM TEAB buffer (625 μL) and labeled with 5 mg of TMT 126 channel reagent (cohort 1 lot# TF266326, cohort 2 lot# SG253447, ThermoFisher Scientific) in anhydrous ACN (256 μL) ...