Labshake search
Citations for Thermo Fisher :
9551 - 9600 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2023Quote: ... An internal standard was prepared from the isotope-labeled standards in 3% acetonitrile with 0.1% formic acid with 5 fmol/µL of Peptide Retention Time Calibration (PRTC) mixture (ThermoFisher Scientific) and 10 µg/mL E ...
-
bioRxiv - Biophysics 2024Quote: ... Individual reactions were heated to 65 °C for 5 min and transferred to ice for 3 min to facilitate annealing in SuperScript III reaction buffer (Invitrogen). After annealing ...
-
bioRxiv - Cell Biology 2023Quote: Sac2 knockdown was performed with siRNA targeting mouse gene sequence Inpp5f: 5’-GGAAUGCGGUAUAAACGAATT-3’ and was from Ambion (Life Technologies). OSBP knockdown was performed using ON-TARGET plus SMART pool Mouse OSBP siRNA from Dharmacon ...
-
bioRxiv - Neuroscience 2023Quote: ... washed in PBS (3 x 5 min) mounted on glass slides (SuperFrost Plus Microscope Slides, ThermoFisher Scientific, Waltham, MA, USA) and left to air-dry overnight ...
-
bioRxiv - Developmental Biology 2023Quote: ... was synthesized on templates that contain the T7 promoter sequence (5’-TAATACGACTCACTATAGGGTACT-3’) at each end using a MEGAscript T7 kit (Ambion); templates were amplified from 0–4 h embryonic cDNA using specific primers (5’-AGCAGATGACGACGACAACA-3 ...
-
bioRxiv - Pathology 2024Quote: ... right arm target 5’-ACA TCG CTA ACC TGC TG CT-3’) were synthesized using the mMESSAGE mMACHINE kit (Ambion) and injected into one-cell stage embryos at 100 pg per embryo ...
-
bioRxiv - Neuroscience 2024Quote: ... Tissues were dissociated using 0.05% Trypsin for 5 min at room temperature and subjected to 3 washes with Hanks’ Balanced Salt Solution (HBSS, Gibco). Cell supernatants were triturated in DMEM media with 20% fetal bovine serum using a 1000 μL pipette and applied to a 100 μm cell strainer (MidSci ...
-
bioRxiv - Cancer Biology 2024Quote: ... Human lung fibroblast MRC5 and its SV40-transformed derivatives were cultured in a 5% CO2 and 3% O2-regulated incubator in MEM medium without Phenol Red (Gibco), completed with 10% FBS ...
-
bioRxiv - Neuroscience 2024Quote: ... Thereafter, sections were rinsed as before (3 x 5 min, cold TBS) then incubated with AlexaFluor conjugated secondary antibodies (Thermofisher) in 5% normal goat serum in TBS for 2 h at 4°C with agitation ...
-
bioRxiv - Neuroscience 2024Quote: ... and Biotinylated Dextran Amine (BDA, cases AV, AT: equal parts 10% 10 kDA and 10% 3 kDA, 5 µl; Invitrogen). Dextran amines with molecular weight 10 kDA (BDA in AT and AV ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were then stained with 12 µl of propidium iodide (PI) red-fluorescent DNA Stain from the 1 mg/ml of stock solution (Thermo Fisher Scientific, G ibco®, Canada). Following this step ...
-
bioRxiv - Neuroscience 2021Quote: ... Plates were then run on either a Viia™ 7 or QuantStudio™ 7 Flex Real-Time PCR Systems (both Applied Biosystems™) with 40 repetition of data collection steps at 60 °C ...
-
bioRxiv - Immunology 2024Quote: ... Real-time RT PCR was performed using ABI ViiA 7 and ABI QuantStudio 7 Flex Systems with MicroAmp® Optical 384-Well Reaction plates with specific barcodes (Life Technologies 4309849) and Power SYBR™ Green PCR Master Mix (Life Technologies 4368708) ...
-
bioRxiv - Cell Biology 2020Quote: ... Total RNA was isolated from duplicate or triplicate samples and concentrations were measured using the NanoDrop One/One Microvolume UV-Vis Spectrophotometer (Thermofisher). CDNA was synthesized using the iScript cDNA synthesis kit (Biorad ...
-
bioRxiv - Genetics 2019Quote: ... For deletions MEL745a cells were transfected with one plasmid targeting upstream and one plasmid targeting downstream sequence surrounding the enhancer using Lipofectamine 3000 (Invitrogen) according to manufacturer instructions ...
-
bioRxiv - Developmental Biology 2021Quote: ... One microgram of total RNA was reverse-transcribed to cDNA using a one-step cDNA Synthesis Kit (Applied Biosystems, USA). Primers used in the study were designed using PrimerBlast tool of NCBI (Table 1) ...
-
bioRxiv - Microbiology 2021Quote: ... one-step quantitative real-time PCR was performed using SuperScript III Platinum SYBR Green One-Step qRT-PCR Kit (Invitrogen) with primers specific for the TRS-L and TRS-B sites for the N gene as well as ACTB as an internal reference ...
-
bioRxiv - Microbiology 2020Quote: ... One-step qRT-PCR was performed using the Invitrogen EXPRESS One-Step Superscript qRT-PCR kit (Thermo Fisher Cat #11781200) and the commercial Taqman probe ACE2 (Hs01085333_m1) ...
-
bioRxiv - Systems Biology 2021Quote: ... The amount and purity of the RNA samples were quantified using a Nanodrop One/OneC Microvolume UV–VIS Spectrophotometer (ND-ONE-W) from ThermoFisher Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... One-step RT-PCR was performed using the Super-Script III One-Step RT-PCR System with Platinum Taq (Invitrogen) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2020Quote: ... one-step quantitative real-time PCR was performed using SuperScript III Platinum SYBR Green One-Step qRT-PCR Kit (Invitrogen) with primers specific for the TRS-L and TRS-B sites for the N gene as well as ACTB as an internal reference ...
-
bioRxiv - Cancer Biology 2022Quote: ... all exosome samples were diluted with one volume of Exosome Resuspension Buffer and one volume of 2X Denaturing Solution (both from Invitrogen, Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... One-step RT-qPCR was performed using One-Step Real-Time RT-PCR Master Mixes (Thermo Fisher Scientific, Waltham, USA) and the StepOnePlus Real Time PCR System (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2022Quote: LLC-MK2 cells were seeded in 2 × 96-well plates (one for inhibition and one for cytotoxicity) in complete media (DMEM from Gibco, Cat ...
-
bioRxiv - Microbiology 2023Quote: ... A total of 8.5 µL extracted RNA was subjected to one-step RT-PCR using the Superscript III one-step RT-PCR system (Invitrogen). The one-step RT-PCR was performed using the forward primer Pol F1 5’-TACAGTGCAGGGGAAAGAATA-3’ (nt 4809-4829 ...
-
bioRxiv - Microbiology 2023Quote: ... A total of 8.5 µL extracted RNA was subjected to one-step RT-PCR using the Superscript III one-step RT-PCR system (Invitrogen). To amplify the pol region ...
-
bioRxiv - Microbiology 2024Quote: ... and used to amplify a 204bp amplicon in a one-step RT-PCR reaction (SuperScript IV One-Step, Thermo Fisher) using custom primers (Table 1) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Using 8 µL Lipofectamine 2000 (ThermoFisher Scientific) this vector ...
-
bioRxiv - Neuroscience 2021Quote: ... in Essential 8 medium (#A1517001; Life Technologies). hESCs were dissociated with Accutase (#AT-104-500 ...
-
bioRxiv - Molecular Biology 2021Quote: ... 8 μl of Streptavidin Magnetic Beads (Invitrogen) were added to capture biotinylated ssDNA ...
-
bioRxiv - Genomics 2020Quote: ... 8 μL anti-V5 (Invitrogen, R960-25). Immunoprecipitation reactions were incubated at 4°C with end-over-end rotation overnight (~16-18 hr) ...
-
bioRxiv - Neuroscience 2020Quote: ... Essential 8 medium (Life Technologies, CA, US) was used for feeder-free culture ...
-
bioRxiv - Microbiology 2019Quote: Cells seeded in 8-chambered slides (Nunc Lab-Tek Chamber Slide system ...
-
bioRxiv - Immunology 2021Quote: ... IL-1β (Invitrogen; sensitivity 8 pg/mL), and IL-33 (Invitrogen ...
-
bioRxiv - Bioengineering 2021Quote: ... The 8-well plates (155409; Thermo Scientific) for HEK293-T and SW480 cells were coated with fibronectin bovine plasma (F1141-2MG ...
-
bioRxiv - Microbiology 2019Quote: ... and cultured in Essential 8 Medium (Gibco) with 100 μg/mL normocin (InvivoGen) ...
-
bioRxiv - Cell Biology 2020Quote: ... and GMP Essential 8 (A1517001, Life Technologies). For routine passaging ...
-
bioRxiv - Neuroscience 2021Quote: ... in Essential 8 Medium (Thermo Fisher Scientific). For neural differentiation ...
-
bioRxiv - Neuroscience 2021Quote: ... using Essential 8™ medium (ThermoFisher scientific) at 37°C ...
-
bioRxiv - Cell Biology 2021Quote: ... supplied with 8% fetal bovine serum (Gibco), 2% chicken serum (Gibco ...
-
bioRxiv - Immunology 2022Quote: ... and 2 mg/ml collagenase 8 (Gibco) for 45 min at 37 °C ...
-
bioRxiv - Genetics 2020Quote: ... with Essential 8 Medium media (Life Technologies), and passaged using EDTA (Life Technologies ...
-
bioRxiv - Cancer Biology 2020Quote: ... purified recombinant human IL-8 (ThermoFisher Scientific) and CXCL1 (Sigma-Aldrich) ...
-
bioRxiv - Systems Biology 2021Quote: ... 20 mM Tris-HCl pH 8 (Invitrogen), 2 mM EDTA (Sigma-Aldrich) ...
-
bioRxiv - Cancer Biology 2020Quote: ... in Essential 8 medium (Thermo Fisher Scientific), while D3 cells and murine iPSCs were cultured on gelatin (Sigma ...
-
bioRxiv - Cell Biology 2021Quote: ... 8 U/mL proteinase-K (ThermoFisher, EO0492)) for 4 h at 37°C for digestion ...
-
bioRxiv - Developmental Biology 2021Quote: ... HuC/D 8 μg/ml (Molecular Probes), Rabbit anti-Foxn4 (1:50 ...
-
bioRxiv - Cell Biology 2020Quote: ... in Essential 8™ medium (ThermoFisher Scientific) and maintained under normoxic conditions in a humidified incubator.
-
bioRxiv - Immunology 2021Quote: ... embedded on an 8 well chamberglass (Nunc) in 50% Matrigel (Corning) ...
-
bioRxiv - Immunology 2022Quote: ... 8% CO2 in Expi293 expression medium (ThermoFisher) supplemented with 10 U/ml penicillin and 10 μg/ml streptomycin ...