Labshake search
Citations for Thermo Fisher :
9851 - 9900 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... Then the samples were washed in 5× SSCT containing DAPI (1:1000) and Alexa Fluor 488-conjugated Wheat Germ Agglutinin (WGA, 5μg/ml, Thermo Fisher Scientific) and with 5× SSCT for 30 min each at room temperature ...
-
bioRxiv - Synthetic Biology 2022Quote: ... The resulting cDNA was diluted 1:10 and 2 μl of each sample was amplified using a QuantStudio 5 (Applied Biosystems) thermal cycler ...
-
bioRxiv - Systems Biology 2019Quote: ... Slides were counterstained with 1 μM DAPI for 5 minutes at room temperature and mounted using Prolong Gold (Life Technologies, P36930). The following antibodies were used ...
-
bioRxiv - Immunology 2019Quote: ... mid-L3 larvae were fed on fly food containing 1 mM 5-ethynyl-2 deoxyuridine (Click-iT EdU, Thermo Fisher Scientific) for 4 hours for hemocyte staining and 3 hours for lymph gland staining at 25°C ...
-
bioRxiv - Cell Biology 2019Quote: ... ELISAs were performed using FBLN5 (Fibulin-5) Human ELISA Kit from Fine Test (EH0772) and Thrombospondin 1 (TSP1) Human ELISA Kit from Invitrogen (BMS2100), following the manufacturer’s protocols ...
-
bioRxiv - Immunology 2019Quote: ... negative murine myeloma cells at ratio 5:1 by slowly adding poly ethylene glycol (PEG) to the cells pellet and Protein Free Hybridoma Media (PFHM) (Gibco, 12040077) supplemented with 20% Heat inactivated Fetal Bovine Serum (FBS ...
-
bioRxiv - Microbiology 2019Quote: ... cDNA was diluted 1:5 in nuclease-free water and qPCR reactions were performed in MicroAmp Fast Optical 96-Well Reaction Plates (Applied Biosystems) using PrimeTime qPCR assays (IDT ...
-
bioRxiv - Cell Biology 2020Quote: iPSC were washed with PBS and harvested by incubating the cells for 5 min at 37°C with 1 mL warm TrypLE Express (Gibco™). The cells and TrypLE were well mixed into a single cell suspension by pipetting up and down and collected in a 15 mL centrifuge tube and diluted 1∶10 with PBS ...
-
bioRxiv - Molecular Biology 2020Quote: ... 5×107 ESCs (untreated and OHT-treated) were crosslinked in 10 ml 1x PBS with 1% formaldehyde (methanol-free, Thermo Scientific) for 10 min at 25°C and then quenched by addition of 125 mM glycine ...
-
bioRxiv - Genomics 2020Quote: ... Nuclei were washed in 1 ml Tagmentation Buffer (see Bulk ATAC-seq above) with 5 μl RiboLock RNase Inhibitor (ThermoFisher EO0381) and centrifuged at 500 xg for 5 min at 4°C ...
-
bioRxiv - Immunology 2020Quote: ... Resulting clones were subjected to surface MR1 5-OP-RU tetramer and monoclonal antibody staining with 1:200 of Live/dead viability dye (Aqua, Life Technologies), 1:25 of anti-CD3 PerCP/Cy5.5 (UCHT1 ...
-
bioRxiv - Cell Biology 2021Quote: ... and permeabilized with cold methanol at −20°C for 2 min or 0.1% TX-100 in PBS for 5 min at room temperature followed by blocking with 0.12% glycine (Fisher Scientific, G46-1) in PBS for 10 min ...
-
bioRxiv - Neuroscience 2021Quote: ... three times and then blocking buffer (5% normal goat serum [Cat #PCN5000, ThermoFisher], 0.2% Triton™ X-100 [Cat #9002-93-1, Fisher Scientific] ...
-
bioRxiv - Microbiology 2020Quote: ... ticks were homogenised in a 2 ml reaction tube with two 5 mm steel beads and 1 ml TRIzol Reagent (Thermo Scientific) using a Tissue Lyser II (Qiagen ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were changed to a fresh medium containing phenol-red-free Williams E supplemented with 5 % CCS and 1 mM glutamine (Invitrogen, CA) and incubated for 22 to 24 hours ...
-
bioRxiv - Immunology 2020Quote: ... splenocytes were isolated from tumor bearing mice and cultured with 4T1 cells at a ratio of 5:1 (splenocytes to tumor cells) in the presence anti-CD107A/CD107B (ThermoFisher Scientific) and Monensin for 4 hours ...
-
bioRxiv - Bioengineering 2019Quote: ... was added at a 1:200 dilution and incubated with cells for 1 h followed by a 20 min incubation with 5 μg/mL Hoechst 33342 (Invitrogen, H1399). HLMVECs were stained for actin with phalloidin conjugated with Alexa Fluor 488 (ThermoFisher Scientific ...
-
bioRxiv - Neuroscience 2019Quote: ... Slices were washed (5x 5 min with PBST) then incubated in secondary antibodies against mouse (1:500 goat anti-mouse, ThermoFisher A11001) and rabbit (1:500 goat anti-rabbit ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were washed three times with PBS and incubated with DAPI for 5 min (1:5,000 in PBS, Life Technologies, 33342) to stain nuclei ...
-
bioRxiv - Cell Biology 2020Quote: ... Live glands requiring staining were treated for 5 min in a 1:100 dilution of LysoTracker Red DND-99 (Life Technologies) in ice-cold PBS.
-
bioRxiv - Biochemistry 2020Quote: ... cells were incubated with an alpha tubulin monoclonal antibody conjugated to Alexa Fluor 488 (B-5-1-2, Invitrogen, Carlsbad, CA) at 2 μg/mL in 1% BSA in PBS for 1 hour ...
-
bioRxiv - Developmental Biology 2020Quote: ... The disrupted spleen tissue was then transferred into a 50ml falcon tube and pipetted up and down in 5 ml of ice cold 1% BSA (Fisher Scientific) in PBS and briefly vortexed to ensure thorough disruption ...
-
bioRxiv - Physiology 2020Quote: First-strand cDNA was made from 1-5 μg total RNA using the SuperScript III First- Strand Synthesis System (Applied Biosystems) per manufacturer’s instructions.
-
bioRxiv - Microbiology 2021Quote: ... cell suspensions concentrated to OD600=5 were spotted at 1 mm distance onto CF 1.5% agar pads and a Gene Frame (Thermo Fisher Scientific) was used to sandwich the pad between the slide and the coverslip and limit evaporation of the sample ...
-
bioRxiv - Biochemistry 2021Quote: 15 pmol of RNA/DNA were radioactively labeled for 1 h at 37 °C using 5 U T4 Polynucleotide Kinase (Thermo Fisher) and 10 µCi γ-32P ATP ...
-
bioRxiv - Microbiology 2021Quote: ... washed four times in PBST for 5 minutes at room temperature with agitation and incubated in secondary antibodies anti-rabbit HRP (1:10000, Invitrogen 31462), anti-bactin HRP (1:5000 ...
-
bioRxiv - Pathology 2021Quote: ... 20,000 cells were co-incubated with 5 × 105 pre-labelled platelets (1:2000 Cell Tracker Deep Red Dye, Thermo Fisher Scientific) in each well of a 96-well plate ...
-
bioRxiv - Cell Biology 2020Quote: ... were grown in a humidified incubator at 37°C and 5% CO2 in DMEM/F12 medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (GIBCO/Life technologies). Cells were plated at approximately 15000 cells/ml on patterned polyacrylamide gels and left to spread for 3 to 4 hours before imaging.
-
bioRxiv - Immunology 2021Quote: ... Monocytes were differentiated into pro-inflammatory (Mφ1) or anti-inflammatory (Mφ2) macrophages with 5 ng/ml of granulocyte-macrophage colony- stimulating factor (GM-CSF; Life Technologies-Invitrogen) or 50 ng/ml macrophage colony- stimulating factor (M-CSF ...
-
bioRxiv - Immunology 2021Quote: ... purified B cells were plated at a concentration of 1×107/ml and cultured with at 37°C with 5% CO2 in RPMI 1640 medium (Gibco, 21875091) supplemented 10% FCS (Gibco ...
-
bioRxiv - Immunology 2020Quote: ... Plates were washed 5 times with washing buffer and developed by addition of 100µL 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo Scientific) for 3 min ...
-
bioRxiv - Immunology 2021Quote: ... The cells were spun at 300g for 10 min with break after which the cells were resuspended into 1-5 ml PBS and filtered using a 100 μm poor size cell strainer and counted on a Countess (ThermoFisher Scientific). The cells were centrifuged at 400g for 10 min with break and resuspended with freeze media (90% FBS supplemented with 10% DMSO ...
-
bioRxiv - Immunology 2020Quote: ... cells were adjusted to 10.106 -20.106 /mL in FACS buffer (PBS, 1% FCS, 5% Hepes, 0.1% Azide) containing SYTOXTM Orange Dead Cell Stain (recommended dilution, Thermo Fisher Scientific) for nuclear staining ...
-
bioRxiv - Immunology 2021Quote: ... cDNA was synthesized using all RNA available (or 1-5 ng) with the High-Capacity Reverse Transcription Kit with RNase Inhibitor (Life Technologies) (250 C for 10 min ...
-
bioRxiv - Microbiology 2022Quote: ... The latter was followed by three times washing with PBS for 5 min and incubation with goat anti-mouse IgG Alexa Flour 488 (1:200 dilution, ref.:A11029, Invitrogen, RRID:AB_138404) for 1 hour ...
-
bioRxiv - Molecular Biology 2022Quote: ... were cultured in DMEM medium supplemented with 10% heat-inactivated FBS and 1% penicillin-streptomycin at 37°C in 5% CO2 (ThermoFisher Scientific). HEK293T cells were cultured in DMEM medium supplemented with 10% FCS and 1% penicillin-streptomycin at 37°C in 5% CO2.
-
bioRxiv - Molecular Biology 2022Quote: ... were cultured in Medium 106 (Cascade Biologics) supplemented with 10% Low Serum Growth Supplement (LSGS) and 1% penicillin/ streptomycin at 37°C in 5% CO2 (ThermoFisher Scientific).
-
bioRxiv - Bioengineering 2022Quote: ... Serial dilutions of gene fragments were performed in sheared salmon sperm DNA at 5 µg mL−1 (m/v) (Thermofisher, LT) diluted in Tris-EDTA (TE ...
-
bioRxiv - Cell Biology 2022Quote: ... once in PBS and resuspended in 1 ml PBS containing 0.5 μg/ml CellMask™ Orange (stock 5 mg/ml in DMSO, ThermoFisher Scientific). Samples were incubated for 5 min at room temperature and subsequently washed three times in PBS ...
-
bioRxiv - Developmental Biology 2022Quote: ... cells were washed 3 times with PBS for 5 minutes and incubated for 1 hour at room temperature with Hoechst 33342 (ThermoFischer Scientific) and specific secondary antibodies (ThermoFisher Scientific) in blocking solution ...
-
bioRxiv - Genetics 2022Quote: ... 10 ng/μl of this linear construct was injected along with 5 ng/μl pharyngeal co-injection marker [myo-2p::mCherry] and 50 ng/μl of 1-kb ladder (Thermo Scientific), with the addition of 10 ng/μl overexpression transgene [azyx-1p::azyx-1(gBlock): ...
-
bioRxiv - Genetics 2022Quote: ... a ben-1 expression plasmid was combined with the myo-2p::GFP co-injection marker (5 ng/uL) and 1 kb DNA ladder (Invitrogen #10787018) at the specified concentrations (Table S2 ...
-
bioRxiv - Genomics 2022Quote: ... was used to enrich poly(A) mRNA from 500 ng total RNA with 5 µL 1:500 diluted ERCC RNA Spike-In control mix (Thermo Fisher) in 50 µL ...
-
bioRxiv - Microbiology 2022Quote: ... 7 µl total RNA was amplified in a mix containing 5 µl of TaqMan Fast Virus 1-step master mix (Life Technologies), 0.4 µl primer-probe mix for IAV 40 and 9.6 µl distilled water ...
-
bioRxiv - Microbiology 2022Quote: ... 5 µl total RNA was amplified in a mix containing 5 µl of TaqMan Fast Virus 1-step master mix (Life Technologies), 1 µl primer-probe mix for β-actin ...
-
bioRxiv - Molecular Biology 2022Quote: ... at a ratio of 5 µL reagent to 1 µg DNA to 100 µL Opti-MEM reduced serum medium (Gibco, 31985062). Approximately 24 h later ...
-
bioRxiv - Molecular Biology 2022Quote: ... at a ratio of 5 µL reagent to 1 µg DNA to 100 µL Opti-MEM reduced serum medium (Gibco, 31985062). Approximately 24 h later ...
-
bioRxiv - Neuroscience 2022Quote: ... We next rinsed 3 x 5 min with PBS and incubated with appropriate secondary antibodies conjugated to Alexa488 or Alexa647 fluorophores (1:250, Molecular Probes) at RT for 45 min ...
-
bioRxiv - Microbiology 2022Quote: ... Membranes were blocked for 30 minutes using 5% skimmed milk in PBST and then probed with rat anti-GFAP primary antibody (1:2000, cat # 13-0300, Thermo Scientific) overnight at 4°C ...
-
bioRxiv - Synthetic Biology 2022Quote: ... a 1:10 dilution was performed and 2 μl of diluted DNA was amplified by means of a QuantStudio 5 (Applied Biosystems) thermal cycler using the SYgreen Blue Mix (PCRbio ...