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Citations for Thermo Fisher :
9801 - 9850 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... 25 μL of magnetic HA beads was washed twice in 1X PBS with 0.1% Tween 20 and then twice in binding buffer (50 mM Tris pH7.5, 150 mM NaCl, 1% IGEPAL CA-630, 5% glycerol, and protease inhibitors (Thermo Fisher #A32955): apoptin ...
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bioRxiv - Systems Biology 2020Quote: ... of the immunodepleted pooled CSF samples were separately dissolved in 100 mM TEAB buffer (625 μL) and labeled with 5 mg of TMT 126 channel reagent (cohort 1 lot# TF266326, cohort 2 lot# SG253447, ThermoFisher Scientific) in anhydrous ACN (256 μL) ...
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bioRxiv - Cancer Biology 2019Quote: ... unmigrated cells were removed from the inside of the inserts with a cotton swab and the cells were fixed in ice-cold 100% methanol for 5 min and stained with 1% w/v crystal violet (ThermoFisher Scientifics) for 3 min ...
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bioRxiv - Biophysics 2019Quote: ... a coverslip containing adherent cells was removed from the cell culture medium and a small piece (0.5-1 cm) was broken off using jewelers forceps (#5) and washed in phosphate buffered saline (PBS, Thermo Fisher Scientific) diluted 1:3 in deionized water ...
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bioRxiv - Immunology 2019Quote: ... cells were harvested from the femur by flushing the bone with 5mL DMEM with 5% FBS and 1% Penicillin Streptomycin (Life Technologies). RBC lysis of the blood was carried out and samples were centrifuged at 450g for 5min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Triton X-100 with 5 % normal-goat-serum and unconjugated F(ab′)2 goat anti-mouse IgG (H+L) antibody (1:500, Thermo Scientific), at 4 °C for 2 h ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies were diluted in PBS-T + 5% NGS as follows: Alexa 555 goat anti-guinea pig (1:200, Invitrogen, #A21435), Alexa 488 goat anti-chicken (1:200 ...
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bioRxiv - Cell Biology 2021Quote: ... and 1% Penicillin Streptomycin at 37 °C and 5% CO2 and split when confluent using Trypsin-EDTA (0.05%) (Life Technologies, 25300054). When indicated ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed three times with PBS and incubated with DAPI for 5 min (1:5,000 in PBS, Life Technologies, 33342) to stain nuclei ...
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bioRxiv - Biophysics 2020Quote: 106 T-cells were incubated in T-cell media supplemented with 5 μg ml−1 Fura-2 AM (11524766, ThermoFisher Scientific) for 20 min at 24°C ...
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bioRxiv - Bioengineering 2020Quote: ... A 1:5 dilution of the PageRuler Plus pre-stained protein ladder was used as a molecular weight standard (ThermoFisher Scientific). Proteins were separated by polyacrylamide gel electrophoresis in 1X SDS running buffer (25 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: Individual preparations of Nef-CD4CD-AP2Δμ2-CTD complex (2.8mg/mL and 0.7mg/mL at 1:5 Nef-CD4CD molar excess) were cross-linked using increasing molar ratios of DSSO (Thermo Fisher Scientific), for 5 ...
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Mediobasal hypothalamic FKBP51 acts as a molecular switch linking autophagy to whole-body metabolismbioRxiv - Neuroscience 2021Quote: ... 50 μl of the re-suspended sample were diluted 1:5 with LC MS-grade water and analyzed using a Dionex ion chromatography system (ICS 5000, Thermo Scientific). The applied protocol was adopted from 72 ...
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bioRxiv - Microbiology 2021Quote: ... 5 µl of TaqMan® RT-PCR mix (TaqMan® RNA-to-CtTM 1-Step Kit) (Applied Biosystems, Waltham, MA, USA), 0.5 µl of TaqMan® RT-Enzyme Mix ...
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bioRxiv - Molecular Biology 2022Quote: ... Triton X-100 in 200 μL of PBS for 5 min and subsequently incubated in 100 μL of click reaction buffer (1× Click-iT cell reaction buffer [Thermo Fisher Scientific] ...
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bioRxiv - Cell Biology 2021Quote: ... plasmid DNA at a concentration of 1-2 mg/ml was mixed with 5 µl of Salmon Sperm DNA (10mg/ml) (Thermofisher Scientific) prior to electroporation ...
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bioRxiv - Cancer Biology 2020Quote: 3×105 viable hCD4+ or mCD8+ T cells were stained with 1 μM of CellTracker Green CMFDA (5-chloromethylfluorescein diacetate; Thermo Fisher) for 5 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... 5 serial dilutions of 1:5 were made for each sample and then quantified using Fast SYBR Green Master Mix (Applied Biosystems) on a 7500 FAST Real Time PCR System (Life technologies) ...
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bioRxiv - Microbiology 2020Quote: ... and 5 μL of resuspended cells were diluted in 1 mL PBS through a 35 μm filter (Corning Falcon tube, Thermo Fisher). FACS DIVA software was used to collect 100,000 events for each sample ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesised from 1-5 μg of template RNA using he Thermo Scientific RevertAid H Minus First Strand cDNA Synthesis Kit by Thermo Fisher Scientific (Waltham ...
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bioRxiv - Microbiology 2020Quote: ... Devices were washed twice with PBS followed by one rinse with 3% NGS plus 0.1% Tween-20 for 5 min each with rocking and secondary antibodies Alexa Fluor 488 goat anti-mouse IgG (Thermo Fisher) and Alexa Fluor 555 goat anti-rabbit IgG (Thermo Fisher ...
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bioRxiv - Systems Biology 2021Quote: ... with an estimated each concentration of 1-5 ng/μl were incubated in the blocking solution with 100-fold diluted SUPERase In RNase Inhibitor (Invitrogen AM2694) at room temperature for 18-24 hours ...
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bioRxiv - Genomics 2021Quote: ... and strained through a 40-μm filter (pluriSelect) then stained for 5 min with Propidium Iodide (1 mg/mL, ThermoFisher Scientific) at room temperature to label dying cells.
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bioRxiv - Molecular Biology 2021Quote: ... The beads were washed five times with lysis buffer containing 1 M NaCl and incubated with 25 µl of biotin elution buffer (lysis buffer with 5 mM D-biotin [Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... siRNA reverse transfection was performed by mixing 1.2 μl siRNA 5 μM with 1 μl lipofectamine RNAiMAX transfection reagent (Invitrogen, ThermoFisher Scientific) to a final volume of 100 μl with Opti-MEM® (Gibco ...
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bioRxiv - Microbiology 2019Quote: ... the reserved 1 mL of ham solution was subjected to decimal dilutions and plated on Columbia agar with 5% sheep blood (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Single cells were stained with anti-mouse fluorochrome-conjugated antibodies: CD4 PE-eFluor 610 (1:400, RM4-5, Thermo Fisher Scientific), CD8a PE (1:200 ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were then washed twice for 5 minutes in PBS and once in PBS with Hoechst 33342 (1:1000, Molecular Probes) before mounting with VECTASHIELD® Antifade Mounting Media (Vector Laboratories) ...
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bioRxiv - Cell Biology 2021Quote: ... incubation with secondary antibody was performed in 0.5% BSA/PBS for 1 hour at room temperature and nuclei were stained with 2 μg/ml Hoechst 33342 (Life Technologies). Images were acquired using the Leica SP8 confocal or Leica DMI6000-SD microscopes ...
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bioRxiv - Cell Biology 2021Quote: ... HEMs were cultured in Medium 254 supplemented with 1% human melanocyte growth supplement (S-016-5, Cascade Biologics/Thermo Fisher Scientific) and 100 units/mL penicillin/streptomycin ...
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bioRxiv - Synthetic Biology 2022Quote: On Day 1: 500 µl of 10 µg/ml S1-hFc or RBD-mFc was coated in a 5 ml immunotube (NUNC, 444202) overnight at 4°C ...
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bioRxiv - Systems Biology 2022Quote: ... secondary antibodies were used for 1 hour at room temperature in 5% BSA and 0.3% Triton-X in PBS: Alexa Fluor 594 conjugated anti-mouse (1:1000; Invitrogen #A-11032) and Alexa Fluor 488 conjugated anti-rabbit (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... 40 cycles of 95°C for 1 s and 60°C for 20 s using the QuantStudio 5 Real-Time PCR System (Applied Biosciences, ThermoFisher Scientific). Specificity was verified by melt curve analysis ...
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bioRxiv - Developmental Biology 2022Quote: ... and dissociated into single cells in 100 μl of Enzyme 1 and 5 μl of Enzyme 2 (Pierce Cardiomyocytes Dissociation Kit, ThermoFisher Scientific) for 30 min at 350 rpm shaking at 30°C ...
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bioRxiv - Genomics 2022Quote: ... heterozygous and knockout colony were expanded in chondrocyte media supplemented with 5 ng/ ml bFGF and 1 ng/ml TGF-β1 (Life technologies) for 11 days ...
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bioRxiv - Molecular Biology 2022Quote: ... were washed three times with Buffer A (PBS pH 8.3, 5% glycerol, 1 mM DTT, 0.03% NP-40, Pierce™ Phosphatase Inhibitor Mini Tablets (Thermo Fisher Scientific)) and incubated with 2 µg of un ...
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bioRxiv - Molecular Biology 2022Quote: ... streptavidin particles with immobilized peptides were washed three times with buffer B (PBS pH 8.3, 5% glycerol, 1 mM DTT, 0.1% NP-40, Pierce™ Phosphatase Inhibitor Mini Tablets (Thermo Fisher Scientific), Roche Complete Mini EDTA-free protease inhibitor tablets (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... in 384 well plates with 0.5 μM forward and reverse primers (sequences in Supplementary table 1) run on a Viia7 Real-Time PCR machine (Thermo Scientific). Fold induction of the target gene was calculated relative to GAPDH in human cells and Hprt in murine cells.
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bioRxiv - Molecular Biology 2022Quote: ... The secondary antibody was Alexa Fluor 549-conjugated goat anti-mouse IgG (H+L) (diluted 1:250 in PBST containing 5% goat serum, Invitrogen, USA) which was incubated overnight ...
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bioRxiv - Neuroscience 2022Quote: Worms were washed with M9 buffer and incubated overnight in 1 ml M9 and 5 μl DiD dye (Vybrant™ DiD Cell-Labeling Solution, ThermoFisher) at ~10-20 rpm ...
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bioRxiv - Cancer Biology 2022Quote: ... and cultured with PDAC tumor organoids in the lower compartment of the 24-well plate in DMEM containing 5% FBS and 1% penicillin/streptomycin (Thermo Fisher). For IL1α neutralization experiments ...
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bioRxiv - Cell Biology 2022Quote: ... 1.5 mM MgCl2, 10mM KCl, 0.1% Nonidet P-40 supplemented with 1 × Halt protease and phosphatase inhibitor cocktails, Thermo Fisher Scientific). One mg total cell protein was used per IP and 1% of the cell lysate volume was loaded as the input to assess the presence of desired proteins in each case ...
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bioRxiv - Biochemistry 2022Quote: ... The secondary antibody (diluted 1:1000 in 5% GS in PBS, Alexa Fluor®488 goat anti-Rabbit, Thermo Fisher Scientific) was added for 1 h RT in dark ...
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bioRxiv - Neuroscience 2020Quote: ... phosphatase inhibitor cocktail sets II and III) (1-5 mg tissue per ml of lysis buffer) and homogenised using a Bead Mill (Fisher Scientific). Laemmli buffer (0.004% bromophenol blue ...
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bioRxiv - Cancer Biology 2020Quote: ... Viable splenocytes were co-cultured with the indicated tumor cell line at a ratio of 1:20 (tumor cell line: splenocyte) in the presence of anti-CD3/ anti-CD28 (5 μg/ml) (Life technologies) for 96 hours at 37°C ...
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bioRxiv - Cell Biology 2021Quote: Cells were set at a concentration of 1 x105 cells/mL 24 hrs prior to EdU addition (5-ethynl-2’-deoxyuridine; Life Technology, Thermo Scientific) in media free from thymidine prepared using Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Microbiology 2020Quote: ... at a flow rate of 5 μL min-1 and separated via a 25 cm analytical column (Acclaim PepMap100 C18, 75 μm × 25 cm, Thermo Scientific) at 35°C using a constant flow rate of 300 nL/min ...
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bioRxiv - Microbiology 2021Quote: ... The cells were then rinsed five times in PBST for 5 min each and counter-stained with 1 μg/ml Hoechst 33342 (Thermo Fisher) for 5 min ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were then washed in 0.1 M TB (2 ×5 minutes) and counterstained with Hoechst 33342 (1:20000, #62249, Thermo Fisher Scientific) diluted in 0.1 M TB ...
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bioRxiv - Microbiology 2022Quote: ... siRNA reverse transfection was performed by mixing 1.2 µl siRNA 5 µM with 1 µl lipofectamine RNAiMAX transfection reagent (Invitrogen, ThermoFisher Scientific) to a final volume of 100 µl with Opti-MEM® (Gibco ...