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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: DAPI (Molecular Probes, D1306, 1 μg/ml) was then added for 5 minutes and washed twice with PBS ...
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bioRxiv - Developmental Biology 2024Quote: E4.5 embryos were fixed in 4% Formaldehyde (Fisher Scientific, PI-28906) at room temperature for 15 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA was quantified using a Qubit™ RNA High Sensitivity (HS) kit (Invitrogen, Q32852). Libraries were prepared from 7 ng of RNA per sample using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB ...
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Self-organization of embryonic stem cells into a reproducible embryo model through epigenome editingbioRxiv - Developmental Biology 2024Quote: ... 1x Non-essential amino acids (Thermo Fisher, 11140050), 1x Pen/Strep and 0.1 mM beta-mercaptoethanol (Thermo Fisher ...
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bioRxiv - Developmental Biology 2024Quote: E6.5 embryos were fixed in 4% Formaldehyde (Fisher Scientific, PI-28906) at room temperature for 30 minutes ...
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Self-organization of embryonic stem cells into a reproducible embryo model through epigenome editingbioRxiv - Developmental Biology 2024Quote: ... 1 ml DMEM+Glutamax (Thermo Fisher Scientific 10566024), 3x Pen/Strep (final concentration 1x ...
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Self-organization of embryonic stem cells into a reproducible embryo model through epigenome editingbioRxiv - Developmental Biology 2024Quote: ... medium was replaced to 2ml DMEM+Glutamax (Thermo Fisher Scientific 10566024) and 10% ESC qualified FBS (MilliporeSigma ...
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Self-organization of embryonic stem cells into a reproducible embryo model through epigenome editingbioRxiv - Developmental Biology 2024Quote: ... 1x Sodium Pyruvate (Thermo Fisher, 11360070), 1x Non-essential amino acids (Thermo Fisher ...
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bioRxiv - Developmental Biology 2024Quote: ... DAPI (Molecular Probes, D1306, 1 μg/ml) was added to blocking buffer and incubated for 2 hours at room temperature before washing 3 times for 15 minutes in blocking buffer and mounted for imaging ...
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bioRxiv - Developmental Biology 2024Quote: ... The samples were placed into a 1.5ml tube with lysis buffer from the Arcturus™ PicoPure™ RNA Isolation Kit (Applied Biosystems, KIT0204), and followed with RNA extraction according to manufacturer’s instructions ...
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Self-organization of embryonic stem cells into a reproducible embryo model through epigenome editingbioRxiv - Developmental Biology 2024Quote: ... diluted in OptiMEM (Thermo Fisher, 31985062). Briefly ...
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bioRxiv - Developmental Biology 2024Quote: ... Triplicate reactions for each sample were prepared and the samples were run in a Step One Real-Time PCR Step One Real-Time PCR System Thermal Cycling Block (Applied Biosystems #4376357). The analysis of real-time data was performed using the included StepOne Software version 2.0 and graphs were generated using GraphPad Prism (GraphPad Software) ...
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bioRxiv - Developmental Biology 2024Quote: ... 50 ng of cDNA of each sample was used in a Maxima SYBR Green/ROX qPCR Master Mix 2x (Thermo Scientific #K0222). Triplicate reactions for each sample were prepared and the samples were run in a Step One Real-Time PCR Step One Real-Time PCR System Thermal Cycling Block (Applied Biosystems #4376357) ...
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Self-organization of embryonic stem cells into a reproducible embryo model through epigenome editingbioRxiv - Developmental Biology 2024Quote: ... supplemented with 1x Pen/Strep (Thermo Fisher Scientific, 15140122), 1uM PD0325901 (StemRD) ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were fixed for 10 minutes with 4% Formaldehyde (Fisher Scientific, PI-28906) at room temperature ...
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Self-organization of embryonic stem cells into a reproducible embryo model through epigenome editingbioRxiv - Developmental Biology 2024Quote: ... in medium consisting of DMEM+Glutamax (Thermo Fisher Scientific 10566024), 1x Pen/Strep (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... Lipofectamine™ LTX Reagent (Catalog No. 15338030, Invitrogen, USA) was used for transfection following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... three siRNAs targeting each were purchased from Ambion (Silencer Select ...
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bioRxiv - Developmental Biology 2024Quote: ... All samples were counterstained with 1:10000 dilution of Hoescht 33342 solution (Invitrogen H3570) in 5ξ SSCT for 5 min at room temperature then washed five times in 5ξ SSCT ...
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bioRxiv - Developmental Biology 2024Quote: ... Tissue was then incubated with 10µg/mL Hoechst 33342 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... coupled to a Q Exactive HF-X hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific). 5 µl of solubilized peptides in solvent A (0.1% formic acid in water ...
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bioRxiv - Cell Biology 2024Quote: ... 10% Fetal Bovine Serum (FBS, Panbiotech) Glutamine (Roth) and Penicilin Streptamycin (Gibco). 500,000 cells were seeded into P6-well plate to reach about 80% confluency on the next day ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration was determined with the Pierce BCA Protein Assay (23227, Thermofisher) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Quantitative RT-PCR was performed using 3uL diluted cDNA (1:5) per reaction and Applied Biosystems SYBR Green PCR Master Mix (Thermo Fisher Scientific; cat:4309155) on a QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were developed using Pierce ECL Western Blotting substrate (32106, Thermo Fisher) and in ECL select Western blotting (GERPN2235 ...
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bioRxiv - Cell Biology 2024Quote: ... Treatment media were Hank’s Balanced Salt Solution (HBSS, Thermo Fisher) for amino-acid starvation and DMEM glucose-free (-Glc ...
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bioRxiv - Cell Biology 2024Quote: ... Amino acid starvation media was prepared based on Gibco standard recipe omitting all amino acids and supplemented as above without addition of non-essential amino acids and substitution with dialysed FBS (Invitrogen). Media was changed 24 h before experiments.
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bioRxiv - Developmental Biology 2024Quote: ... on a QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific). Primer sequences are indicated in Appendix Table 2 ...
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bioRxiv - Cell Biology 2024Quote: ... and stable glutamine (GlutaMAX, Thermo Fisher). Bafilomycin A1 (Santa Cruz ...
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bioRxiv - Developmental Biology 2024Quote: ... and incubated with the appropriate fluorescent secondary antibodies AlexaFluor 488 (Invitrogen), AlexaFluor 594 (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: LC-MS/MS measurements were performed on an EASY-nLC 1000 nano-flow UHPLC system (Thermo Fisher Scientific) coupled to a Q Exactive HF-X hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2024Quote: ... and then homogenized in TRIzol (Thermo Fisher Scientific) using a tissue lyser and RNA was extracted using TRIzol-chloroform extraction ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA was isolated and purified using a PureLink RNA Mini Kit (Ambion; Carlsbad, CA, USA ; cat:12183025). Conversion into cDNA was performed using reverse transcription (iScript cDNA Synthesis Kit ...
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bioRxiv - Developmental Biology 2024Quote: ... and anti-Ezrin (Invitrogen; Burlington ...
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bioRxiv - Developmental Biology 2024Quote: ... AlexaFluor 594 (Invitrogen), and/or stained with 0.17µM phalloidin-iFluor 594 (AAT Bioquest ...
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bioRxiv - Developmental Biology 2024Quote: mES cells were cultured in DMEM-GlutaMAX (Gibco) supplemented with 15% FCS ...
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bioRxiv - Developmental Biology 2024Quote: ... at a density of 9×104 cells ml−1 in N2B27 differentiation medium containing Advanced DMEMF12 and Neurobasal media (1:1, Life Technologies), B27 w/o vitamin A (Life Technologies) ...
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: ... hPSCs were cultured on Geltrex (Thermo Scientific) coated dishes in combination with fully defined mTeSR1 medium (Stem Cell Technologies) ...
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bioRxiv - Cell Biology 2024Quote: ... total RNA was extracted from trophozoites of gene silenced and control strains that were cultivated in the logarithmic phase using TRIZOL reagent (Life Technologies, California, USA). Approximately 1 µg of DNase treated total RNA was used for cDNA synthesis using Superscript III First-Strand Synthesis System (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... penicillin and streptomycin (Life Technologies). On day 2 ...
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: ... cDNA samples were diluted 5-folds and PCR was performed with StepOne Plus Real-Time PCR System (PE Applied Biosystems). The FastStart Universal SYBR Green Master (Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... and Alexa Fluor 488-568- or 647-conjugated phalloidin (Life Technologies) were used 1:200 ...
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bioRxiv - Developmental Biology 2024Quote: ... 2 mM L-glutamine (Life Technologies), 0.1 mM β-mercaptoethanol ...
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bioRxiv - Cell Biology 2024Quote: ... and Blue were purchased from Thermo Fisher Scientific (Massachusetts ...
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: The RNA was extracted with mirVanaTM miRNA Isolation Kit (Ambion, Thermo Fisher Scientific) following the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2024Quote: ... Approximately 1 µg of DNase treated total RNA was used for cDNA synthesis using Superscript III First-Strand Synthesis System (Thermo Fisher Scientific, Massachusetts, USA) with reverse transcriptase and oligo (dT ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA (2 μg) was reverse transcribed with a SuperScript III Reverse Transcription kit (Applied Biosystems, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... qRT-PCR was performed using TaqMan Fast Advanced Master Mix (Applied Biosystems) and TaqMan gene assays (AR Hs00171172_m1 ...
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: ... cDNA was generated using SuperScript™ II Reverse Transcriptase (Thermo Scientific) and miRNA-specific stem-loop primers as previously described [14] ...
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bioRxiv - Molecular Biology 2024Quote: ... The standard phenol/chloroform (ThermoFisher, 10308293) process was used to extract DNA for the following qPCR ...