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Citations for Thermo Fisher :
1201 - 1250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... deposited by Blau H.M.) and the secondary antibody was an Alexa Fluor 488-conjugated anti-mouse antibody (Invitrogen). Nuclei were counter-stained with DAPI (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... non-essential amino acids (Thermo Fisher Scientific, 11140050), 2mM L-glutamine (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... a 20μl system of ChamQ SYBR Master Mix (Low ROX Premixed) (Q331, Vazyme, China) is employed on the ABI QuantStudio5 System(Thermo Fisher, USA). GAPDH is chosen as the internal reference gene ...
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bioRxiv - Molecular Biology 2024Quote: ... transfection (2μg plasmid) followed by 500 μg/ml hygromycin B (Gibco, 10687010) selection for 10 days before being single-cell sorted ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 mM sodium pyruvate (Thermo Fisher Scientific, 11360070), and 100 μM 2-mercaptoethanol (MP Biomedicals ...
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bioRxiv - Molecular Biology 2024Quote: ... using the Bolt Mini Blot Module (Life Technologies) and western blot carried out using the iBind Western Blot Apparatus (Life Technologies) ...
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bioRxiv - Molecular Biology 2024Quote: ... Each sample was cut into smaller pieces and placed into gelatin-coated tissue culture dishes with DMEM (Thermo Fisher Scientific, 10567022) supplemented with antibiotics ...
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bioRxiv - Molecular Biology 2024Quote: ... where targeted vision genes were PCR-amplified in 16 cycles after capture with streptavidin beads (Dynabeads, ThermoFisher). Once amplified ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was purified by phenol/chloroform (pH 7.0) (ThermoFisher, 10308293) and ethanol precipitation ...
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bioRxiv - Molecular Biology 2024Quote: ... 1μL RNase A (10 μg/ml) (Thermo Scientific™, EN0531) and 2 μL Proteinase K (10 mg/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... or mock were cultured in DMEM media (Gibco, #11965-084) supplemented with puromycin and 10% FBS (Atlas Biologicals ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1µL/sample Turbo DNase in 1X Turbo DNase Buffer (100µL/sample)(Thermo fisher, AM2238) was added to digest chromatin pellets at 37°C with 1000rpm shake until pellets were dissolved ...
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bioRxiv - Molecular Biology 2024Quote: ... nuclear lysates of inputs and affinity-purified elution were loaded on a 4-20% Tris-glycine gel (Invitrogen, Cat# XP04205BOX), 10% acetic acid and 50% methanol was used to fix the gel in for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... 11873580001)/1X phosphatase inhibitors (Thermo Fisher, A32961)(PPI)] and 1μL/sample of Benzonase® Nuclease (Merck ...
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bioRxiv - Microbiology 2024Quote: ... and EDTA-free protease inhibitor cocktail (Thermo Fisher J61852.XF). Parasites were lysed by 10 passages through a syringe fitted with a 25-gauge needle and 20 passages with a 27-gauge needle ...
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bioRxiv - Cancer Biology 2024Quote: ... and then treated with 0.2 × SSC buffer (Ambion, AM9763) with gentle shaking at room temperature for 20 min ...
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bioRxiv - Cancer Biology 2024Quote: ... and then mounted with ProLong™ Gold Antifade Mountant with DAPI (Invitrogen, P36931). For each experiment ...
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bioRxiv - Cancer Biology 2024Quote: ... sections were incubated with Alexa-488 and Alexa-546-conjugated secondary antibodies (Invitrogen, A32731 and A11126), mounted with ProLong™ Gold Antifade Mountant with DAPI ...
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bioRxiv - Cancer Biology 2024Quote: ... and Dulbecco’s modified Eagle medium (Gibco, Cat. #12100046) respectively ...
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bioRxiv - Cancer Biology 2024Quote: ... Epithelial organoids were further digested by 0.05% pre-warmed Trypsin (Life Technologies, 25300), washed in ice-cold Hanks Balanced Salt Solution (StemCell Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... Collagenase IV (NC9836075, Thermo Fisher Scientific), DNase I ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by magnetic bead clean-up (Dynabeads MyOne Silane, 37002D, Thermo Fisher Scientific). 10X-barcoded full-length cDNA was then amplified by PCR and analysed using Bioanalyzer High Sensitivity DNA kit (5067-4626 ...
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bioRxiv - Microbiology 2024Quote: ... Subsequent cDNA was analyzed in technical triplicate through quantitative PCR using SYBR Green fluorescent probe with ROX (Invitrogen) in a QuantStudio 7 qPCR instrument (Applied Biosystems) ...
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bioRxiv - Microbiology 2024Quote: Primary CF-HBECs from 4 donors were polarized on 24 mm Transwell filters and washed with DPBS (ThermoFisher Scientific) before treatment ...
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bioRxiv - Microbiology 2024Quote: ... The cells were cultivated in 24-well plates using Dulbecco’s Modified Eagle Medium (DMEM, Gibco, USA) supplemented with 10% Fetal bovine serum (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... incubated with secondary antibodies (donkey anti-mouse 488 Invitrogen, A-21202, donkey anti-rabbit 568, Invitrogen, A-10042, donkey anti-goat 647, Invitrogen, A-21447) for 2 hours at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... 1% antibiotic mixture (penicillin-10,000 U/ml and streptomycin-10 mg/ml, ThermoFisher, USA), and 1% non-essential amino acids mixture (Life Technologies ...
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bioRxiv - Microbiology 2024Quote: ... in a QuantStudio 7 qPCR instrument (Applied Biosystems). Cp values for each target gene (primers HCCS_qF1 ...
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bioRxiv - Microbiology 2024Quote: ... 50 µg/ml of hygromycin B (ThermoFisher, USA), and kept in a 5% CO2 atmosphere at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... and total RNA was extracted using the RNeasy Plus Mini kit (Ambion, Lithuania). The RNA was quantified using a Biophotometer (Eppendorf ...
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bioRxiv - Microbiology 2024Quote: ... developed using the SuperSignal West Pico PLUS enhanced chemiluminescence reagent (Thermo Fisher 34577), and imaged on an Invitrogen/Thermo Fisher iBright 1500 imager.
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bioRxiv - Microbiology 2024Quote: ... 20 µg of mitochondrial protein was mixed with 1X Native-PAGE sample buffer (Invitrogen BN2003) supplemented with 1% digitonin and incubated on ice for 15 min ...
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bioRxiv - Microbiology 2024Quote: ... and the reverse transcription was performed using Superscript FirstStrand Synthesis for RT-PCR (Invitrogen, CA, USA). cDNA was amplified using the Master Mix kit (Thermofisher ...
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bioRxiv - Microbiology 2024Quote: ... adapters were ligated to the amplicons in a PCR reaction using the P5 and P7-bearing adapter primers and the Phusion High-Fidelity DNA Polymerase kit (Thermo Scientific). Amplicons were sized and purified using an AmpurXP magnetic bead capture (Agencourt) ...
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bioRxiv - Microbiology 2024Quote: ... (Gibco BRL, ThermoFisher Scientific Inc., Waltham, MA, USA) followed by the addition of two-fold serially diluted RDE-treated ferret sera at 50 µL per well starting with 1:20 dilution in virus growth medium containing 1 µg/mL TPCK-treated trypsin ...
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bioRxiv - Microbiology 2024Quote: ... Plates were washed five times with PBS and then twice with distilled water (dH2O) before addition of 5-bromo-4-chloro-3-indolyl-phosphate/NBT (BCIP/NBT) one-step solution (Thermo Fisher Scientific) and incubation at 37°C for approximately 15 minutes ...
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bioRxiv - Microbiology 2024Quote: ... The samples were stored in RNA Later solution (Invitrogen) at -70 °C until use ...
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bioRxiv - Microbiology 2024Quote: Vero E6 cells (ATCC C1008) were maintained in Eagle’s minimum essential medium (Gibco; Thermo Fisher Scientific, MA, USA) supplemented with 5% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2024Quote: ... and 20 U/L of turbo DNase (Ambion). Then ...
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bioRxiv - Microbiology 2024Quote: Vero E6 cells (ATCC C1008) were maintained in Eagle’s minimum essential medium (Gibco; Thermo Fisher Scientific, MA, USA) supplemented with 5% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2024Quote: ... (Gibco BRL ...
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bioRxiv - Microbiology 2024Quote: ... The non-capsid-protected DNA was eliminated with 2.5 units per mL of DNase I following the manufacturer’s procedure (Cat 18047-019, Invitrogen, MA, USA). The treated VLPs were then concentrated with AMICON and recuperated in 200 μL SM buffer ...
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bioRxiv - Microbiology 2024Quote: Hela cells were cultured in DMEM supplemented with 10% foetal bovine serum (Invitrogen). Plasmids pEGFPC1 and pEGFPN1 ...
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bioRxiv - Microbiology 2024Quote: ... The quality of RNA was assessed using a NanoDrop (ND-1000; Thermo Scientific, USA). cDNA was synthesized using 1 µg of RNA ...
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bioRxiv - Microbiology 2024Quote: ... 1% insulin-transferrin-selenium (Gibco; Thermo Fisher Scientific), 1% penicillin (50 units/mL) ...
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bioRxiv - Cell Biology 2024Quote: ... 1% NEAA (Non-Essential Amino Acid; Gibco; #11140050) and 0.1 mM beta-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: ... which was then amplified in MAX Efficiency DH5α Competent Cells (Invitrogen).
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 0.5% N-2 supplement (Gibco), 0.5% B-27 supplement (Gibco) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed twice with DPBS (Dulbecco’s phosphate buffered saline, Gibco) before medium replacement ...
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bioRxiv - Cell Biology 2024Quote: ... we transfected Sox2se-CPG-TV-dTomato and Cdk1-Snrpn-dTomato plasmids into mouse iPSCs using Lipofectamine 2000 Transfection Reagent (Invitrogen) according to the provider’s protocol ...