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Citations for Thermo Fisher :
9901 - 9950 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... to a final concentration of 1% at RT for 15 min and quenched by addition of glycine (ThermoFisher, AJA1083) to a final concentration of 150 mM for 5 min at RT ...
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bioRxiv - Microbiology 2022Quote: ... Reverse transcription RT-qPCR data were collected and analyzed using the QuantStudio Design and Analysis (version 1.5.2; Applied Biosystems) software package ...
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bioRxiv - Developmental Biology 2022Quote: ... Total RNA (200 ng) was reverse transcribed into cDNA using the RevertAid RT Reverse Transcription Kit (ThermoFisher Scientific – K1622). For microRNA ...
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bioRxiv - Microbiology 2022Quote: ... The RNA resulting from selective depletion was used for random-primed cDNA synthesis using the SuperScript IV RT (Invitrogen). Second-strand cDNA was generated using Escherichia coli DNA ligase ...
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bioRxiv - Microbiology 2024Quote: Extracted RNA was subjected to RT-qPCR using the TaqMan RNA-to-CT 1-Step Kit (ThermoFisher, Waltham, MA) in a 20μL reaction with 0.5μM of primers and 0.1μM of probe labeled with FAM and ZEN/Iowa Black quenchers (IDT ...
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bioRxiv - Cell Biology 2024Quote: ... 0,5µM Smartseq3 OligodT30VN (IDT; 5’-Biotin-ACGAGCATCAGCAGCATACGAT30VN-3’) adjusted to RT volume and 0,5mM dNTPs/each (Thermo Fisher, #R0181). After cell sorting lysis plates were centrifuged before storage at -80°C ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were then incubated for 15 minutes at RT with Syto9 and/or PI (Thermo-Fisher Scientific, Waltham, Massachusetts) at 20 and 60 µM final concentration in 0.85% sodium chloride buffer ...
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bioRxiv - Microbiology 2024Quote: Ligation reactions were cleaned up with the Oligo Clean & Concentrator Kit and reverse transcribed using Superscript IV RT (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: RNA extraction from phNPCs and reverse transcription (RT) were performed with Power SYBR Green Cells-to-CT kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... KORD-injected brain sections were incubated overnight at RT in Rabbit Anti-GFP Polyclonal Primary Antibody (Invitrogen, #A-11122) in 1% BSA + 0.2% Triton X-100 in 0.01 M PBS (1:1,000 ...
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bioRxiv - Microbiology 2024Quote: ... Extracted RNAs were subjected to reverse transcription using the Invitrogen SuperScript IV VILO Master Mix RT kit (Life Technologies) or QuantiTect Reverse Transcription kit (Qiagen) ...
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bioRxiv - Cell Biology 2024Quote: ... and RT was performed with 1-2 μg RNA using SuperScript™ IV VILO™ Mastermix (11756050, Thermo Fisher) with the following conditions ...
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bioRxiv - Developmental Biology 2024Quote: ... the samples were incubated for 90 minutes at RT with anti-mouse-IgG2a-Alexa647 (RRID:AB_2535810, A21241, Invitrogen, 1:800), anti-mouse-IgG1-Alexa555 (RRID:AB_2535769 ...
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bioRxiv - Microbiology 2024Quote: First strand cDNA was synthesized using Maxima First Strand cDNA Synthesis Kit for RT-qPCR with dsDNase (ThermoFisher Scientific). Template RNA (50 ng ...
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bioRxiv - Genomics 2023Quote: ... cDNA synthesis was performed in 2.5 μg total RNA with 260/280 nm ratio >1.8 following the manufacturer’as instructions for the Maxima First Strand cDNA Synthesis Kit for RT-qPCR (Thermo Fisher), employing random primers and oligo dT.
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bioRxiv - Developmental Biology 2022Quote: ... organoids were mechanically disrupted with the P1000 pipette tips first and then trypsinized with RT TrypLE Express (Gibco, 1952062) for up to 15 min to disassociate into single cells ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated in a humid chamber for 1 hour at RT with 30 μl of secondary antibody donkey anti-rabbit AlexaFluor488 (1:1.000, Invitrogen) in 3% BSA/PBS additionally containing 1 μg/ml DAPI for visualization of nuclei ...
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bioRxiv - Microbiology 2023Quote: ... and incubated with Alexa488-labelled goat anti-mouse IgG (A11001, Thermo Fisher Scientific, 5 μg/mL, 45 min, RT), and titres were calculated by the modified Kärber method ...
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bioRxiv - Plant Biology 2022Quote: ... was used for RT-qPCR and runs performed in 384 well plates (AB3384, Thermo Fisher Scientific, Waltham, MA, USA) in 5 μl reactions ...
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bioRxiv - Pathology 2023Quote: ... We performed the LN34 and β-actin RT-qPCRs on a QuantStudio 7 Flex (ThermoFisher Scientific, Waltham, MA, USA) as described previously [13 ...
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bioRxiv - Neuroscience 2023Quote: ... were incubated for 2 h at RT and the proteins were detected with Supersignal West Pico (34580, Thermo Scientific) and imaged by using iBright 1500 (Invitrogen) ...
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bioRxiv - Physiology 2023Quote: ... Total RNA (1 µg in 20µl) was reverse transcribed using reverse transcription (RT) kits (Applied Biosystems; Foster City, CA) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... incubated 1 hour at RT in a solution of DAPI 1/1000 and fluorophore-coupled secondary antibodies (Thermo Fisher) diluted in PBT ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... RT-qPCR cycle quantity thresholds were measured using the ThermoFisher Scientific TaqMan Advanced Master Mix (Applied Biosystems: Cat. #4444556) and TaqMan primers on the Bio-Rad CFX95 instrument (Supplemental Table 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... while the cDNA synthesis was performed with the Maxima First Strand cDNA Synthesis Kit for RT-qPCR (Thermo Scientific). Real-time RT-PCR was performed with LightCycler® 480 II (Roche ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were stained for 45 minutes at RT using fluorescence-labelled antibodies and Calcein Violet (#C34858, Thermo Fisher Scientific) for cell viability ...
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bioRxiv - Immunology 2023Quote: ... we added 7 µl of the RT mix that contained 0.5 µl of 200 U/µl Superscript IV (Invitrogen), 0.25 µl of 40 U/µl RNAseOUT (ThermoFisher Scientific) ...
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bioRxiv - Plant Biology 2023Quote: ... RT-qPCR was performed using a LightCycler 96 and the PowerUp SYBR green master mix (Applied Biosystems, Massachusetts, USA). Gene expression levels were normalized to the reference genes RCE1 (AT4G36800 ...
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bioRxiv - Cancer Biology 2022Quote: ... RT-qPCR was performed using PowerUp Syber Green Master Mix with 10 ng of cDNA added per reaction (Invitrogen). RT-qPCR thermocycling conditions are as follows ...
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bioRxiv - Biophysics 2023Quote: ... Secondary antibody staining was performed for 1 hour at RT in blocking solution (AF647 anti-rabbit #A21244, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA synthesis from 1.5 or 2 μg RNA was performed using the High Capacity cDNA RT Kit (Applied Biosystems). Quantitative RT-PCR experiments were run on a C1000 Touch Thermal Cycler (BioRad ...
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bioRxiv - Cancer Biology 2022Quote: ... single-cell suspensions were fixed for 15 minutes at RT in 2% paraformaldehyde (methanol free, Thermo Scientific, J19943-K2), and permeabilized for 15 minutes at RT in Intracellular Staining Perm Wash Buffer (Biolegend ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... plates were thawed on ice and each well was supplemented with 0.2 ul SuperScript III RT Platinum Taq Mix (Invitrogen), 2.8 ul DNA suspension buffer (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... Secondary antibody incubations were performed for 45 minutes at RT with both anti-rabbit-Alexa Fluor-546 (Invitrogen #A11035) and anti-mouse Alexa Fluor-488 (Invitrogen #A21202 ...
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bioRxiv - Neuroscience 2023Quote: ... with PBS + Triton 0.1% at RT and exposed to anti-mouse Alexa Fluor 488-conjugated goat secondary antibodies (1:500; Invitrogen) for 1h at RT ...
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bioRxiv - Physiology 2023Quote: ... RT-qPCR was conducted with TB Green Premix Ex Taq II using the ABI Prism 7000 thermocycler (Life Technologies). PCR conditions were as follows ...
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bioRxiv - Neuroscience 2023Quote: ... The RT-qPCR was performed using the Promega® 2x Master Mix and the QuantStudio™ 6 (Applied Biosystems) for 40 cycles ...
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bioRxiv - Evolutionary Biology 2023Quote: ... corresponding secondary antibodies were added for 1h at RT: (Alexa-594-conjugated goat anti-rabbit IgG (H+L) (Invitrogen), Alexa-594-conjugated goat anti-chicken IgG (H+L ...
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bioRxiv - Plant Biology 2023Quote: ... RT-qPCR was performed using the ChamQ Universal SYBR qPCR Master Mix (Vazyme, Q711-02) by QuantStudio5 (Applied biosystems). The expression of interested genes was normalized to Tubulin for calibration ...
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bioRxiv - Cancer Biology 2024Quote: ... and incubated for 2h at RT with secondary antibody mix of Alexa Fluor 647 anti-rat (Invitrogen, A-21247) and Alexa Fluor 488 anti-mouse (Invitrogen ...
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bioRxiv - Physiology 2024Quote: ... and incubated for 1h at RT with a chicken anti-mouse antibody conjugated to Alexa Fluor 647 (Life Technologies, Thermo Fisher ...
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bioRxiv - Developmental Biology 2023Quote: ... a 10-min incubation at RT with streptavidin associated with peroxidase (UltraVision Detection System HRP kit, Thermo Fisher Scientific) was performed ...
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bioRxiv - Molecular Biology 2023Quote: ... and blocked for 1 h at RT in PBST containing 10% of fetal bovine serum (FBS) (Thermo Fisher Scientific). Cells were subjected to immunostaining overnight at 4°C with primary antibodies (Table S3 ...
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bioRxiv - Biochemistry 2023Quote: ... were incubated for 1 hour at RT and coverslips were mounted with ProLong Diamond Antifade reagent (Thermo Fisher Scientific). Images were acquired on a Leica TCS-SP5-II-RS-WLL equipped with a 100x ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was reverse-transcribed using Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase (RT) according to the manufacturer’s instructions (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were stained for 45 min at RT using fluorescence-labelled antibodies and Calcein Violet (#C34858, Thermo Fisher Scientific) for cell viability ...
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bioRxiv - Microbiology 2023Quote: ... cDNA synthesis and RT qPCR analysis was performed using Maxima H Minus First Strand cDNA Synthesis Kit (Thermo Fisher) and Maxima SYBR Green/Fluorescein qPCR Master Mix (2X ...
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bioRxiv - Biophysics 2023Quote: ... RT-qPCR was carried out on a Roche LC480 using the PowerUp SYBR Green Master Mix (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were washed 3 times with TBS-T and incubated 1h at RT with an HRP-conjugated anti-mouse secondary antibody (dilution 1:50,000, G-21040, Invitrogen). After 3 washes in TBS-T ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed and reacted for 1h at RT with appropriate secondary antibodies (1:1,000; goat anti-rabbit secondary antibody Alexa Fluor 546; #A-10040, ThermoFisher Scientific ...