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Citations for Thermo Fisher :
9801 - 9850 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Reverse-transcription (RT) was performed with 500 ng of RNA using random hexamers with the SuperScript III kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... The RT-qPCR experiments were performed using Maxima SYBR Green/ROX qPCR Master Mix (Thermo Scientific, Baltics, UAB, Lithuania) on a Light Cycler 480 Roche ...
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bioRxiv - Microbiology 2022Quote: Duplicate no RT (NRT) control reactions were assembled for each sample containing only Platinum Taq DNA polymerase (Thermofisher 15966005) instead of the Superscript III RT/Taq DNA polymerase mix ...
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bioRxiv - Neuroscience 2021Quote: RT-qPCR was carried out with PowerUp™ SYBR™ Green Master Mix (2x) (Thermo Fisher Scientific; Monza, Italy) for the septum and with SsoAdvanced™ Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCRs were performed in duplicate using Taqman Gene Expression Assays and Taqman Gene Expression Master Mix (Applied Biosystems) with GAPDH as an internal control ...
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bioRxiv - Immunology 2019Quote: ... First-strand cDNA generation was performed with 100ng of isolated total RNA using a SuperScript RT II kit (Invitrogen) and oligo-dT primer ...
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bioRxiv - Neuroscience 2021Quote: ... 0.1% Triton X-100 secondary antibody for 60 minutes at RT (goat-anti-rabbit Alexa 546, 1:500, ThermoFisher A-11035 ...
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bioRxiv - Molecular Biology 2021Quote: ... and quantified by RT-qPCR using the PowerUp™ SYBR™ Green Master mix (Cat#A25742; Thermo Fisher Scientific) on a StepOne Plus RealTime PCR system (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2021Quote: RT reactions were carried out using the superscript III first strand synthesis system (Thermo Fisher Scientific, Waltham, MA, USA). cDNA was synthesized starting from 2 μg of purified total RNA ...
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bioRxiv - Genomics 2020Quote: RT-qPCR was performed on cDNA obtained from total RNA using the High-Capacity cDNA Reverse Transcription Kit (ThermoFisher), using the primers detailed in Supplementary Table S3 ...
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bioRxiv - Immunology 2021Quote: ... All RT-qPCR experiments were performed with TaqMan gene expression assays and TaqMan gene expression master mix (Applied Biosystems) on the Applied Biosystems StepOne real-time PCR system ...
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bioRxiv - Cell Biology 2019Quote: ... RT-qPCR was performed according to the manufacturer’s protocol (technical duplicates) using TaqMan® Gene Expression Assays (Applied Biosystems). Primers for hypertrophic differentiation and (cartilaginous ...
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bioRxiv - Pathology 2019Quote: ... 1 μg of total RNA samples were reverse transcribed using the M-MLV-RT system (Invitrogen™, Shanghai, China). The program was performed at 42 °C for 1 h and terminated by deactivation of the enzyme at 70 °C for 10 min ...
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bioRxiv - Cell Biology 2019Quote: ... Genomic DNA was eliminated by treatment with DNAse I for 20 min at RT using DNAse IH (Invitrogen, USA). RNA concentration was measured using a Nanodrop ND-1000 spectrophotometer (NanoDrop Technologies ...
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bioRxiv - Molecular Biology 2019Quote: ... for 20 min at RT and reverse transcribed using the Maxima First-Strand cDNA Synthesis Kit (K1671, ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... overnight at RT followed by Alexa Fluor 488 goat anti-rabbit IgG (H+L) (1:250, #A11008, Life Technologies), Alexa Fluor 647 donkey anti-goat IgG (H+L ...
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bioRxiv - Neuroscience 2019Quote: ... overnight at RT followed by Alexa Fluor 488 goat anti-rabbit IgG (H+L) (1:250, #A11008, Life Technologies), Alexa Fluor 635 goat anti-mouse IgG (H+L ...
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Definition and Signatures of Lung Fibroblast Populations in Development and Fibrosis in Mice and MenbioRxiv - Cell Biology 2020Quote: ... GEM-reverse transcription (RT) was performed in a Veriti 96-well thermal cycler (Thermo Fisher Scientific, Waltham, MA, USA). GEMs were collected and the cDNA was amplified and purified with SPRIselect Reagent Kit (Beckman Coulter ...
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bioRxiv - Plant Biology 2019Quote: ... RT-qPCR reactions were carried out in a StepOneTM 7500 real-time cycler (Applied Biosystems, Foster City, California, EUA). One specific primer pair was designed to amplify MIR from all species evaluated (F ...
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bioRxiv - Plant Biology 2019Quote: ... Expression studies were carried out by real-time reverse transcription polymerase chain reaction (RT-qPCR; StepOne plus, Applied Biosystems) using the Power SyBR Green master mix (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2020Quote: ... RT was performed using TaqMan™ Reverse Transcription Reagent or TaqMan™ Advanced miRNA cDNA Synthesis Kit (Thermo Fisher). qPCRs were performed using GoTaq® Probe qPCR Master Mix (Promega ...
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bioRxiv - Genomics 2019Quote: ... Then 25 µg of RNA were reverse transcribed into cDNA using 1.8 kU SuperScript II RT (Invitrogen #18064-014), 12.5 ng/µL random hexamers ...
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bioRxiv - Microbiology 2019Quote: ... First-strand cDNA synthesis was performed with the Maxima First Strand cDNA Synthesis Kit for RT-qPCR (Thermo Fisher), and real-time qPCR was performed with SYBR Green Master Mix (Bio-Rad ...
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bioRxiv - Cell Biology 2019Quote: ... RT-qPCR reaction was performed using 5 ng of cDNA and 2x SYBRGreen master mix (Thermo Fisher Scientific, USA) on a BioRad thermocycler (BioRad ...
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bioRxiv - Neuroscience 2019Quote: ... The neurons on coverslips were stained with 0.4% trypan blue for 15 min at room temperature (RT) and then washed with Dulbecco’s phosphate buffered saline (D-PBS; Invitrogen). Trypan blue dye itself is a cell impermeable but only can enter the cells with compromised membrane ...
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bioRxiv - Immunology 2019Quote: ... Reverse transcription was carried out in a master mix of Maxima RNaseH-minus RT enzyme and buffer (Fisher Scientific), MgCl2 (Sigma) ...
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bioRxiv - Biophysics 2020Quote: ... and incubated for 30 min at room temperature (RT) with Image-IT FX Signal Enhancer (Life Technologies, catalogue #I36933) to neutralize surface charge ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were washed three times in 0.1% BSA in PBS and incubated with the corresponding secondary antibodies at 1:500 dilution for 1.5 hrs at RT (Alexa Fluor 488 and 647 nm; Invitrogen). Cells on glass coverslips were mounted with mounting media (Invitrogen ProLong Gold Antifade Reagent) ...
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bioRxiv - Systems Biology 2020Quote: ... Reverse transcription was carried out in a master mix of Maxima RNaseH-minus RT enzyme and buffer (Fisher Scientific), MgCl2 (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were then incubated for 1.5 h at RT with a Alexa 488 or Cy3-conjugated secondary antibody (1:300; Invitrogen). The stained sections were examined with a confocal microscope and images were captured with a CCD spot camera ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR reactions were performed with Taq-Man™ Fast virus 1-step master mix (ThermoFisher Scientific, Cat. 4444436) and primer-probe sets targeting SARS-CoV-2 nucleocapsid (N1 set ...
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bioRxiv - Neuroscience 2020Quote: ... hCMEC/D3s were washed three times with room temperature (RT) PBS and lysed using Pierce RIPA buffer (Thermo Scientific) containing 1x Halt protease inhibitor cocktail (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Viral or host RNA levels were determined using the TaqPath™ 1-Step RT-qPCR Master Mix (ThermoFisher # A15299) on CFX Connect Real-Time System (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription (RT) of total RNA was performed using the Superscript III First Strand Synthesis Kit (Thermo Fisher Scientific) with random hexamers according to the provided protocol ...
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bioRxiv - Microbiology 2020Quote: ... the cells were washed in PBS and incubated for 1-h at RT with 1 mL anti-Early Endosomal Antigen 1 (EEA1) monoclonal antibody (F. 43.1; Invitrogen) diluted 1:200 in blocking buffer ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then rinsed for 5 min at RT (3X) with PBS and incubated with secondary antibody (Alexa Fluor 647 diluted 1:2,000 in blocking buffer; Invitrogen) for 45 min at RT ...
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bioRxiv - Plant Biology 2021Quote: ... and cDNA was synthesized using the Maxima first-strand cDNA synthesis kit for RT-qPCR with DNase (Thermo Scientific) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... RT-qPCR was performed according to the Power SYBR Green Master Mix protocol (Life Technologies, Thermo Fisher, United States), using a Rotor-Gene Q machine (Qiagen ...
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bioRxiv - Pathology 2021Quote: Cryosections were permeabilized and blocked for 1 h at RT in 0.1% PBTx and 10% fetal calf serum (FCS; Invitrogen), and incubated O/N at 4 °C with the primary antibodies in 0.1% PBTx plus 1% FCS ...
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bioRxiv - Cancer Biology 2020Quote: ... converted to cDNA by RT with random hexamers and Moloney murine leukemia virus (MuLV) reverse transcriptase (Thermo Fisher Scientific) and used in PCR with the following primers ...
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bioRxiv - Microbiology 2021Quote: A reverse transcription (RT) reaction was performed in 50 μL containing 5 μL of 10× SuperScript III buffer (Invitrogen), 5 μL of 50 mM MgCl2 ...
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bioRxiv - Cell Biology 2020Quote: ... RT-qPCR was carried out using TaqMan Gene Expression Assays and TaqMan Master Mix (Applied Biosystems, Foster City, CA). 18S (Hs03003631_g1 ...
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bioRxiv - Cell Biology 2020Quote: ... Digestion was performed for 45 min at RT and then stopped with Superase-In RNase Inhibitor (Thermo Scientific #AM2696) for 10 min on ice ...
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bioRxiv - Cell Biology 2020Quote: ... samples were washed 3X with PBST at RT for 1 h and mounted with SlowFade Gold antifade reagent (ThermoFisher). Samples were analyzed using a Nikon inverted TE2000-E microscope equipped with phase contrast and epifluorescence ...
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bioRxiv - Cancer Biology 2021Quote: ... RT-qPCR was performed using TaqMan® probes and Gene Expression qPCR Assays TaqMan Gene Express (ThermoFisher Scientific, 4369016) with 40 cycles on an ABI PRISM 7700 (Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... click reaction was performed previously to immunofluorescence during 30 minutes at RT with 1 µM Alexa488-azide (Invitrogen, A10266). Images were acquired in a Zeiss LSM880 confocal microscope and analysed using Fiji software.
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bioRxiv - Neuroscience 2022Quote: ... following 1h incubation at RT with corresponding Alexa 488/Cy3/Cy5 coupled secondary antibodies (1:1000, Thermo Fisher Scientific). Cell nuclei were stained with DAPI ...
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bioRxiv - Cell Biology 2022Quote: ... After incubation cells were incubated for 5 min at RT with TMR-phalloidin (0.83 μg/mL) and Hoechst 33342 (1 μg/mL, Invitrogen) in PBS and washed with blocking buffer before imaging on a Zeiss Axiovert 200 equipped with an Andor Luca camera.
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR was performed using Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix (Applied Biosystems™ A25918), Applied Biosystems™ QuantStudio 3 real-time PCR system ...
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bioRxiv - Cell Biology 2022Quote: Isolated colonic crypts or organoids were loaded at RT with Fura-2/AM (5 μm, 2h; Thermo Fisher Scientific) in HBS ...