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Citations for Thermo Fisher :
9851 - 9900 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and incubated for 1h at RT with AlexaFluor488-conjugated donkey anti-mouse IgG secondary antibody (0.2 µg/ml; ThermoFisher) diluted PBS-HS ...
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bioRxiv - Plant Biology 2021Quote: ... RT-qPCR was performed according to the Power SYBR Green Master Mix protocol (Life Technologies, Thermo Fisher, United States), using a Rotor-Gene Q machine (Qiagen ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 3X washes with PBST and 10 min incubation at RT with 1:10000 of DAPI (ThermoFisher scientific) in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... Detection was performed with a 1h incubation at RT with secondary antibodies AlexaFluor488 or AlexaFluor594 (1:500; Molecular Probes), and DAPI (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... 4μl of bisulfite converted RNA were subjected to RT with High Capacity cDNA reverse transcription kit (Applied Biosystems #4368814) according to manufacturer procedure and incubated with the following program ...
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bioRxiv - Molecular Biology 2020Quote: ... TaqMan RT qPCR analysis was carried out using the ABI prism 7500 Sequence Detection System (Applied Biosystems, CA, USA) with specific FAM-labeled probes for cIAP2(Birc2 ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR was performed as previously described using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher, Cat#4444436) and an OneStepPlus Real-Time PCR System (96-well format ...
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bioRxiv - Immunology 2021Quote: ... then treated with DNAse I at room temperature (RT) prior to incubation with 5 µM Sytox Green (Thermo Fisher) and quantification via fluorimetry using a Perkin Elmer plate reader ...
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bioRxiv - Microbiology 2020Quote: ... and then incubated for 10 minutes at RT in the same buffer containing 100 μg/ml lysozyme (Thermo Scientific) and 1000 U/ml nuclease (BenzonaseNuclease ...
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bioRxiv - Bioengineering 2021Quote: ... equal amounts of RNA were reversely transcribed to cDNA using high-capacity cDNA RT kit (Applied Biosystems, ThermoFisher Scientific) as per instructions ...
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bioRxiv - Bioengineering 2021Quote: ... equal amounts of RNA were reversely transcribed to cDNA using high-capacity cDNA RT kit (Applied Biosystems, ThermoFisher Scientific) as per instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei were stained with DAPI at RT for 30 sec and sections mounted with ProLong Gold antifade reagent (Invitrogen) were placed on the slide and covered with coverslips.
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bioRxiv - Biophysics 2021Quote: ... Immunolabeled platelets on coverslips were incubated at RT with linking solution (0.1 mg/ml Acryloyl-X in PBS, A20770, Invitrogen) for 12 h ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA was reverse transcribed in a final volume of 20 μL using 0.5 μg of oligo dT and 200 U Superscript III RT (Invitrogen) for 30 mins at 50 °C ...
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bioRxiv - Neuroscience 2022Quote: ... cells were permeabilized and blocked for 1 h at RT using 0.1% Triton X-100 (Thermo Fisher Scientific, 28313) and 4% normal donkey serum (Jackson Immuno Research ...
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bioRxiv - Neuroscience 2022Quote: ... cells were exposed for 1h at RT to secondary antibodies: Goat anti-chicken AlexaFluor 488 (1:1000, ThermoFisher, A21131), Donkey anti-rabbit AlexaFluor 555 (1:1000 ...
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bioRxiv - Pathology 2022Quote: ... RNA was reverse transcribed and amplified using the TaqMan Fast Virus 1-Step Master Mix RT-qPCR kit (Invitrogen) on the LightCycler 480 or LC96 instrument (Roche ...
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bioRxiv - Microbiology 2022Quote: ... Viral RNA was amplified by RT-qPCR using the Taqman Fast Virus 1-step master mix (Thermo Fisher Scientific). Primers and probes targeting SARS-CoV-2 RNA-dependent RNA polymerase were described in [25] ...
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bioRxiv - Biochemistry 2019Quote: ... RNA extraction and subsequential RT-qPCR analysis of chosen gene expression level was performed according to manufactures’ instruction (Ambion Power SYBR Green Cells-to-CT Kit (ThermoFisher Scientifics)) ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were then washed twice in PBS-T and incubated at RT with the anti-rabbit PE antibody (Thermofisher) (1/200 in PBS-T/5% BSA ...
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bioRxiv - Physiology 2020Quote: ... 12.5 ng of the RT reaction product was amplified in duplicate using Fast SYBR Green master mix (Applied Biosystems). Real-time PCR was performed on a StepOne Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Systems Biology 2019Quote: ... One microliter of RT-RamDA mix (2.5× PrimeScript Buffer, 0.6 pmol oligo(dT)18 (Cat# SO131, Thermo Fisher Scientific), 8 pmol 1st-NSRs [30] ...
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bioRxiv - Immunology 2020Quote: ... were incubated for 1 hour at RT with 100 μl/well of solution in PBS of 0.5 % laboratory-grade gelatin (J.T. Baker Chemicals, Fisher Scientific). After 3 washes with deionized water ...
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bioRxiv - Neuroscience 2020Quote: ... cells were permeabilized and blocked for 1 h at RT using 0.1% Triton X-100 (Thermo Fisher Scientific, 28313) and 4% donkey serum in PBS ...
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bioRxiv - Microbiology 2021Quote: The expression of miRNAs was assessed using RT-qPCR with the TaqMan Advanced miRNA cDNA Synthesis Kit (Applied Biosystems) in conjunction with Taqman Advanced miRNA assays (Applied Biosystems ...
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bioRxiv - Molecular Biology 2019Quote: ... 25 mM DTT and 1.25 mM dNTPs) and 1 µL of Superscript II RT (Thermo Fisher, 200 U/µL) for 42 °C for 3 hours ...
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bioRxiv - Cell Biology 2021Quote: ... and immunolabeled with 10 μg/ ml anti-CD4 (Q4120) for 1.5 hr at RT followed by detection with secondary antibodies conjugated to Alexa Fluor dyes (Invitrogen). Finally ...
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bioRxiv - Cell Biology 2021Quote: ... and immunolabeled with primary antibodies (anti-Gag [SIV 27c] and/or anti-Nef [KK77]) for 1.5 hr at RT followed by secondary antibodies conjugated to Alexa Fluor dyes (Invitrogen) for 1 hr at RT ...
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bioRxiv - Microbiology 2021Quote: ... Twenty microliter reactions were made using TaqPath™ 1-Step RT-qPCR Master Mix (ThermoFisher Scientific, Waltham, MA, USA), with 10 µmol 1:1:1 ratio of forward/reverse primer ...
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bioRxiv - Genomics 2019Quote: ... Single-stranded cDNA was synthesized from 1 μg of RNA using a High capacity cDNA RT kit (Thermofisher # 4368814), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... secondary antibodies coupled to horseradish peroxidase were added for 1 h at RT before ECL detection (Thermo Fisher Scientific). Immunoblots were quantified using ImageJ.
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bioRxiv - Immunology 2021Quote: ... Viral genomes were quantified via RT-qPCR with the TaqMan RNA-to-Ct 1-step kit (ThermoFisher, Cat # 4392653) and primer/probe sets with the following sequences ...
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bioRxiv - Genomics 2021Quote: ... centrifuged at 500xg for 5 minutes at 4°C using a swinging bucket rotor (Sorvall Legend RT, Thermo Fisher), and then pellets were washed with an additional 1 mL cold lysis buffer and incubated on ice for an additional 5 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... Actin filaments were fluorescently labeled with AlexaFluor-488-conjugated phalloidin (Life Technologies; 1: 200 dilution, 1 h at RT) for visualization ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were incubated for 30min at RT with goat anti-rabbit-Alexa Fluor 635 antibody (1:400, Molecular probes), Proteostat 1:400 and Hoechst 1:800 (ENZO ...
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bioRxiv - Microbiology 2020Quote: ... the qPCR technique was carried out using the Verse 1-step RT-qPCR SYBR Green ROX kit (Thermo Scientific) and the protocols recommended by the manufacturer of the LightCycler® 96 termal cycler (Roche) ...
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bioRxiv - Microbiology 2021Quote: One microgram of DNA-free RNA was reverse transcribed using random hexamers and Superscript III Reverse Transcriptase (RT) (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Retro-transcription (RT) and qPCR reactions were then performed using the Power SYBR Green Cells-to-Ct kit (Ambion), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... samples were washed 3X with PBST at RT for 1 h and mounted with SlowFade Gold antifade reagent (ThermoFisher). Samples were analyzed using a Nikon inverted TE2000-E microscope equipped with phase contrast and epifluorescence ...
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bioRxiv - Neuroscience 2021Quote: ... which were then blocked during 1h at RT with Superblock™ in PBS blocking buffer (Thermo fisher Scientific #37515) or PBS containing 0.1% Tween 20 and 3% BSA ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA was spectrophotometrically quantified and equal amounts were used for cDNA synthesis with the Superscript II RT Kit (Invitrogen). qPCR analysis was performed on an ABI Real Time PCR System (Applied Biosystems ...
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bioRxiv - Molecular Biology 2022Quote: ... One microgram of the extracted RNA was used for reverse transcription (HIGH CAPACITY CDNA RT; Applied Biosystems ref: 4368814). Transcribed cDNA was diluted 5-fold and used for real-time quantitative PCR (QuantiTect SYBR Green ...
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bioRxiv - Developmental Biology 2022Quote: ... and 5 μl of the RT reaction was amplified in 25 μl using AccuPrime Pfx DNA polymerase (Thermo Fisher, 12344024 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Total RNA was used to make cDNA under RNAase-free conditions with the Ambion RETROscript RT kit (Invitrogen AM1710). A 12μl reaction using 2μl RNA and 1.2 μM oligo(dT ...
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bioRxiv - Genomics 2022Quote: ... Relative mtDNA copy number of the post-adapter ligated sample was then determined via RT-qPCR (Applied biosystems StepOnePlus). This critical step determined the amount of post-ligated DNA to use as input for the pre-capture indexing PCR to optimize the number of Duplex Consensus Sequences formed for each Unique Molecular Identifier (57) ...
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bioRxiv - Plant Biology 2023Quote: ... RT-qPCR were performed using SsoAdvanced Universal SYBR Green Supermix (Bio Rad) on a StepOne Plus apparatus (Applied Biosystems) using standard protocols (40 to 45 cycles ...
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bioRxiv - Immunology 2022Quote: ... RT-qPCR was performed as previously described using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher, Cat#4444436) and a OneStepPlus Real-Time PCR System (96-well format ...
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bioRxiv - Neuroscience 2023Quote: ... 2 µl of RT mix (1x SuperScript IV buffer, 18 U/µl SuperScript IV reverse transcriptase (Thermofisher Scientific, USA), 3.6 µM TSO-chimera primer ...
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bioRxiv - Neuroscience 2023Quote: ... Expression of Cbln1 and other target genes was quantified using RT-qPCR with Power SYBR Green Master Mix (ThermoFisher) and a Bio-Rad CFX 384 Real-Time System ...
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bioRxiv - Molecular Biology 2022Quote: ... to a final concentration of 1% at RT for 15 min and quenched by addition of glycine (ThermoFisher, AJA1083) to a final concentration of 150 mM for 5 min at RT ...