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Citations for Thermo Fisher :
9501 - 9550 of 10000+ citations for TRAP 5 amide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were washed with PBS-0.1%Tríton-X three times (5 minutes each) and incubated with the secondary antibodies (1:2000; Alexa-Fluor conjugated; Invitrogen), at RT for 1h ...
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bioRxiv - Genomics 2024Quote: ... St Jude Children’s Research Hospital) cells were cultured in culture medium F12 media with 2.5% or 5% heat inactivated fetal bovine serum (GIBCO) and Penicillin-Streptomycin (GIBCO) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were cultured in 6-well plates at 37 °C in a 5% CO2 atmosphere and split every 72 hours by trypsinization (0.25%, Gibco, ThermoFisher). Stable HEK293 lines were generated by lipofection (Lipofectamine 2000 ...
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bioRxiv - Bioengineering 2023Quote: ... 5 × 105 HSkMC cells were incubated with 2 μg of eGFP-mRNA packaged in LPP or lipofectamine (Thermo Scientific) for 24 h in 6-well plate ...
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bioRxiv - Cell Biology 2024Quote: HeLa cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12) with Glutmax and 5% FCS (Gibco) and passaged every few days ...
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Reduction of chromosomal instability and inflammation is a common aspect of adaptation to aneuploidybioRxiv - Cell Biology 2023Quote: ... Cells were cultured at 37C at 5% CO2 in Advanced Dulbecco’s Modified Eagle Medium: Nutrient mixture F-12 (DMEM-F12) with Glutamax (GIBCO), supplemented with 12% FCS (Clontech) ...
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bioRxiv - Cell Biology 2024Quote: ... transient transfections of 5 μg of pcDNA3.1-Foxm1 were performed using 25 μg of Lipofectamine 2000 (Thermo Fisher Scientific). After 24 h ...
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bioRxiv - Developmental Biology 2024Quote: ... at a ratio of 5 uL Lipofectamine™ 2000: 2.5 ug plasmid DNA diluted in Opti-MEM (Gibco: 31985062). After 48 hrs ...
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bioRxiv - Developmental Biology 2024Quote: ... qPCR was performed using the SYBR Select Master Mix and a QuantStudio 5 Real-Time PCR System (Applied Biosystems). Primers against specific target genes are listed in Supp ...
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bioRxiv - Genetics 2024Quote: ... Tissues were rehydrated with PBS and blocked for 1 hour at room temperature with 5% normal goat serum ((Gibco) source ...
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bioRxiv - Immunology 2024Quote: ... and the RT-PCR reaction was performed using a Quant Studio 5 Real-Time PCR Instrument (Applied Biosystems, MA). For quantification ...
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bioRxiv - Genomics 2024Quote: ... 5-10 μg of antibody was coupled to 50-100 μl of Protein A or Protein G Dynabeads (Invitrogen). The following antibodies were used ...
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bioRxiv - Genetics 2024Quote: ... Cells were cultured at 37C at 5% CO2 under humidity in myoblast proliferation medium [1Vol medium 199 (ThermoFisher, 41150020) + 4 vol DMEM (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: Both 3D7 and IT4 parasite lines were maintained following standard procedures at 5% hematocrit in RPMI 1640 medium supplemented with 0.5% Albumax II (Invitrogen) in an atmosphere containing 5% oxygen ...
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bioRxiv - Microbiology 2024Quote: ... were then mixed 1:1,000 with 5 mL of MMB agar + Tetracycline and poured into 4-well Nunc Rectangular Dishes (ThermoFisher). Dishes were dried at room temperature for 1 hour ...
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bioRxiv - Microbiology 2024Quote: ... falciparum (3D7, Dd2, and HB3) parasites were cultured in 3-5% human O+ RBCs in RPMI-1640 (Gibco, 110875093) complete medium containing 0.5% Albumax-II (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... Colon was further cut into 5-10 mm pieces and put into a 15 mL falcon tube with 5 mL prewarmed 2 mM EDTA in Hank’s balanced salt solution (HBSS, Gibco) and shaken at 250 rpm for 15 min at 37°C ...
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bioRxiv - Immunology 2024Quote: ... comprising 2 μl sample DNA and 48 μl reaction mixture containing per reaction 5 μl PCR buffer 10x (Invitrogen), 2 μl MgCl2 (50 mM) ...
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bioRxiv - Biochemistry 2023Quote: ... heated for 5 minutes at 95°C and loaded on 4-12% Bis-Tris NuPage gels (Thermo Fisher Scientific). After electrophoretic separation ...
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bioRxiv - Biochemistry 2023Quote: ... 5 μL of milk was diluted with 50 μL of Tris-Glycine SDS Sample Buffer (Novex, Thermo Fisher Scientific) and 35 μL of milli-Q water ...
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bioRxiv - Bioengineering 2023Quote: ... Mice were sacrificed and spleens were removed and placed in 5 mL of Hanks Balanced Salt Solution (ThermoFisher, 24020117). Using small razors ...
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bioRxiv - Synthetic Biology 2023Quote: WT HeLa (ATCC CCL-2) were cultured at 37 °C with 5% CO2 in flasks with Dulbecco’s modified Eagle medium (DMEM) (Gibco) supplemented with 1 mM L-glutamine (Gibco) ...
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bioRxiv - Systems Biology 2023Quote: ... We quantified cDNA abundance using quantitative-polymerase chain reaction (qPCR) using the QuantStudio 5 Real-Time PCR system (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... and cell membranes were labeled with 5 µg/ml fluorescent disordered/nonraft phase marker FAST DiO (Thermo Fisher Scientific) for 10 min on ice ...
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bioRxiv - Bioengineering 2024Quote: ... and 72°C extension for 30 sec by using QuantStudio™ 5 Real-Time PCR System (A28574, Applied Biosystems). The expression levels of targeted genes were normalized using the housekeeping gene glyceraldehyde 3-phosphate dehydrogenase (GAPDH) ...
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bioRxiv - Bioengineering 2024Quote: ... and forward/reverse primers from IDT Technology in the QuantStudio™ 5 Real-Time PCR System (Applied Biosystems; A28574). The cycle was run 40 times at 95°C denaturation for 30 sec ...
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bioRxiv - Cell Biology 2024Quote: ... half of the media was replaced with fresh HoxB8 Media Culture containing 5 µg/mL blasticidin-HCl (ThermoFisher A1113903) for selection ...
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bioRxiv - Cell Biology 2024Quote: ... 0,5µM Smartseq3 OligodT30VN (IDT; 5’-Biotin-ACGAGCATCAGCAGCATACGAT30VN-3’) adjusted to RT volume and 0,5mM dNTPs/each (Thermo Fisher, #R0181). After cell sorting lysis plates were centrifuged before storage at -80°C ...
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bioRxiv - Biochemistry 2024Quote: ... were obtained from Janvier and infected with 5×103 AnTat1.1E trypanosomes (intraperitoneally (i.p.) in 200 µL HBSS (Hanks’ balanced salt solution, ThermoFisher Scientific).
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bioRxiv - Bioengineering 2024Quote: ... and forward/reverse primers from IDT Technology in the QuantStudio™ 5 Real-Time PCR System (Applied Biosystems; A28574). The cycle was run 40 times at 95°C denaturation for 30 sec ...
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bioRxiv - Biochemistry 2024Quote: To perform DSF, 5 µM protein was mixed with substrate (0, 1, or 15 mM) and 2.5x SYPRO Orange dye (ThermoFisher), and made up to a total volume of 50 µl with DSF buffer (50 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2024Quote: ... 2,000 cells were dispensed in 5 μl of medium using Multidrop Combi (Thermo Fisher Scientific Inc., Waltham, MA, USA). The final concentration of the screened compounds was 10 μM ...
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bioRxiv - Cancer Biology 2024Quote: ... plastic flat bottom 96 well plates were coated with 5 μg/mL human fibronectin in PBS (GIBCO, 14080-055) for 1h in 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Each peptide extract was loaded on a 300 μm ID x 5 mm PepMap C18 precolumn (Thermo Scientific, USA) at a flow rate of 10 μL/min ...
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bioRxiv - Cancer Biology 2024Quote: ... After 15 minutes of incubation with 5 nM SYTOX™ Red Dead Cell Stain (#S34859, Invitrogen, Carlsbad, CA, USA) on ice ...
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bioRxiv - Biophysics 2023Quote: MDCK cells were cultured at 37 °C in an atmosphere of 5% CO2 in DMEM (1X) + Glutamax (Thermo Fisher) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... or CDLK5 in 10 mM HEPES-NaOH pH 7.4 and 500 mM NaCl was incubated with B1 (12.5, 25, or 50 µM) in the presence of 5× SyPRO orange dye (Invitrogen). Next ...
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bioRxiv - Bioengineering 2023Quote: ... and rho kinase inhibitors such as a CEPT cocktail consisting of 5 µM Emricasan (50-136-5235, Fisher Scientific), 50 nM Chroman 1 (7163 ...
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bioRxiv - Microbiology 2023Quote: ... and incubated with Alexa488-labelled goat anti-mouse IgG (A11001, Thermo Fisher Scientific, 5 μg/mL, 45 min, RT), and titres were calculated by the modified Kärber method ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplification reactions were monitored for 5 hours at 30 °C in MicroAmp fast reaction tubes with cap (Applied Biosystems) and using real-time PCR machine (Applied Biosystems).
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bioRxiv - Molecular Biology 2023Quote: ... Human dispersed islets were seeded at a density of 5×104 cells/cm2 on ultra-low attachment plates (ThermoFIsher) and allowed to form spontaneous pseudoislets ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting single cell suspension was washed three time with 5 ml of warm Neurobasal Plus medium (Gibco, A3582901) containing B27 Plus supplement (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... were maintained at 37°C in 5% CO2 in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Thermo Fisher), 2 mM Glutamax ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were incubated for 5 hrs before the media was changed to media + 1% Calcein AM stain (Thermo Fisher) and incubated for a further hr ...
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bioRxiv - Bioengineering 2023Quote: ... qPCR was performed with PowerTrack SYBR green master mix (A46109) by using QuantStudio™ 5 qPCR system (ThermoFisher, A34322) with specific primers (Table 2) ...
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bioRxiv - Microbiology 2023Quote: ... 20 μl resuspended parasite culture were incubated with dihydroethidium (5 μg/ml, Cayman) and SYBR Green I dye (0.25 x dilution, Invitrogen) in a final volume of 100 μl medium for 20 min at room temperature protected from light ...
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bioRxiv - Molecular Biology 2023Quote: ... scraped into 6 ml Farnham lysis buffer (5 mM PIPES pH 8.0, 85 mM KCl, 0.5% Igepal CA-630) with protease inhibitors (ThermoFisher, # A32953), transferred to 15 ml Falcon tubes ...
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bioRxiv - Microbiology 2023Quote: ... Secondary antibodies were prepared in 5% goat serum in blocking buffer: goat anti-mouse 594 (AlexaFluor Invitrogen, 1:2000) and goat anti-rabbit 488 (AlexaFluor Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... the slides were directly subjected to background blocking using the Ultra Vision Protein Block for 5 min (ThermoFisher Scientific) and exposed to primary and secondary antibodies for 45 min and 30 min at RT ...
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bioRxiv - Microbiology 2023Quote: ... were routinely cultivated at 37°C with 5% CO2 in Dulbecco’s modified Eagle medium (DMEM; Gibco, Dun Laoghaire, Ireland) supplemented with 10% FBS ...