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Citations for Thermo Fisher :
9601 - 9650 of 10000+ citations for TRAP 5 amide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... with a multiwavelength UV-Vis photodetector and a C18 Hypersil BDS column (4.6 x 150 mm, 5 µm, ThermoFisher). The chromatographic analysis was carried out using mobile phase A (0.1 % TFA in water ...
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bioRxiv - Physiology 2023Quote: Amplicons for dsRNAs were designed using the SnapDragon dsRNA design tool (https://www.flyrnai.org/snapdragon) and in vitro transcribed (IVT) using MEGAscript® T7 Transcription Kit (Cat# AMB1334-5, ThermoFisher). IVT reactions were carried out as per the protocol provided by the DRSC (available at ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 5% FBS and antibiotics (penicillin 100 U ml−1, streptomycin 100 U ml−1 (pen/strep) (Gibco), 10mM glutamax (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... The established stable cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Nacalai Tesque) supplemented with 4∼5% fetal calf serum (FCS; Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... the sample was washed 3 x 5 min with PBS and mounted in Fluoromount-G (ThermoFisher, 00-4958-02). 15 samples per genotype were prepared and photographed using a tile scan at a confocal microscope Zeiss LSM780 (Zeiss ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were grown on gelatinised culture plate at 37C and 5% CO2 in medium containing Dulbecco’s Modified Eagle Medium (Gibco) with 10% foetal bovine serum (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... and lectin microarrays) were carried out in the same mediums with 5% dialyzed Fetal Bovine Serum (FBS) (Gibco, A3382001) replacing the standard HS used for MCF10A culture ...
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bioRxiv - Cell Biology 2023Quote: ... boiled at 95°C for 5 min and loaded into a NuPAGE 3-8% Tris-Acetate Gel (Thermo Scientific) along with HiMark™ Pre-stained Protein Standard (Thermo Scientific LC5699) ...
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bioRxiv - Cell Biology 2023Quote: ... the samples were incubated with DAPI (1/25,000 in PBS) for 5 min and mounted with Prolong Diamond antifade mounting medium (Invitrogen). The samples were imaged and analyzed with C-Plan Apochromat 63x/1.4 Oil DIC M27 objective under confocal microscope (LSM780NLO ...
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bioRxiv - Cancer Biology 2023Quote: ... cultured at 37°C in a humid 5% CO2 atmosphere in either the RPMI 1640+GlutaMAX medium (Life Technologies) supplemented with 10% fetal bovine serum and 2 nM DHT (AD-cells ...
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bioRxiv - Biochemistry 2023Quote: ... citri inocula OD600nm was normalized to 0.05 and incubated for 5 days at 30°C in a laminated microscopy chamber (Nu155411; Lab-Tek, NUNC). Biofilm images were acquired using a Nikon Eclipse Ti microscope equipped with a 100x magnification objective (CFI Plan Apo Lambda 100XH ...
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bioRxiv - Biophysics 2023Quote: ... The resulting cell pellets were resuspended in FACS buffer (PBS supplemented with 0.5% (w/v) BSA and 0.1% (w/v) sodium azide and counted using an Attune NxT Flow Cytometer (ThermoFisher). Gating for live cells with moderate levels of the GFP were used to compare MHC-I expression ...
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bioRxiv - Cell Biology 2023Quote: ... approximately 1 μg of peptides were loaded on a C18 precolumn (PepMap100, 5 μm, 300 Å, Thermo Fisher Scientific) and separated over a C18 column (50 cm × 75 μm ...
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MET functions in tumour progression and therapy resistance are repressed by intronic polyadenylationbioRxiv - Molecular Biology 2023Quote: 5 µg of total RNA was used for poly(A)+ RNA purification with MicroPoly(A)Purist™ Kit (Ambion). Poly(A)+ RNA was fragmented at 70°C for 10 min in RNA Fragmentation buffer (Ambion) ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative PCR was then performed using EvaGreen Master Mix (Solis BioDyne) detected by the QuantStudio 5 and analyzed using QuantStudio Software v1.5.2 (Applied Biosystems). Primer sequences used for qRT-PCR are shown in supplementary materials and methods ...
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bioRxiv - Molecular Biology 2023Quote: ... concentrated over an Acclaim PepMap C18 pre-column (5µm particle size, 0.3mm ID x 5 mm length, ThermoFisher Scientific), then bound to an EasySpray C18 column (2 µm particle size ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blocked with 5% bovine serum albumin in TBST and biotin was detected with Streptavidin-HRP (ThermoFisher).
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bioRxiv - Microbiology 2023Quote: ... Metabolites were detected and quantified as area under the curve based on retention time and accurate mass (5 ppm) using the TraceFinder 4.1 software (ThermoFisher).
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bioRxiv - Microbiology 2023Quote: ... IL-5 and IL-13 concentration were assessed following manufacturer’s instructions using mouse uncoated IL-5 and IL-13 ELISA kits (Invitrogen).
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bioRxiv - Molecular Biology 2023Quote: ... Fibroblasts were cultured at 37° C at 5% CO2 in Dulbecco’s modified Eagle’s medium with nutrient mixture F-12 (Gibco-11330) and supplemented with 15% fetal bovine serum ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... sections were washed three times for 5 min in PBS (0.1 M, pH 7.4) and incubated with rabbit anti-GFP (1:500; Invitrogen, A11122) and chicken anti-GFAP (1:500 ...
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bioRxiv - Genomics 2023Quote: ... HB-8065) were cultured in a humidified incubator at 5% CO2 at 37 C using high-glucose DMEM (Invitrogen) supplemented with 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... cDNA was synthesized and quantitative PCR performed using SYBR Green Express and a QuantStudio 5 qPCR instrument (Life Technologies). Primers were:
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bioRxiv - Microbiology 2023Quote: ... or BSA (control, 5 µg/mL, Roth, Karlsruhe, Germany) in colorless RPMI (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) for 2 h at 37 °C ...
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bioRxiv - Genomics 2023Quote: cDNA was generated from 5 μg of RNA per sample using the SuperScript IV First-Strand Synthesis System (Invitrogen). A VHL-specific primer complementary to the gene’s 3’ UTR was used for priming the reaction ...
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bioRxiv - Immunology 2023Quote: ... and cellular debris spun down at 12,000 g for 5 minutes before being subjected to electrophoresis on Bolt 4- 12% Bis-Tris gels from Invitrogen/Thermo Fisher (Cat ...
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bioRxiv - Immunology 2023Quote: ... Slides were washed extensively (3 x 5 mins) in TBS-tween20 and incubated in appropriately labeled secondary antibodies (Invitrogen) for 1 h at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Following preset incubation times (0, 0.5, 1, 3, or 5 h) cells were harvested by trypsinization (500 μl TrypLE, Gibco) for 5 min at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... washed in 1xTBS and then incubated for 5 min with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher, 34577). The chemiluminescent signal was scanned with UVP ChemStudio (Analytik Jena) ...
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bioRxiv - Microbiology 2023Quote: ... coverslips were washed once with 0.1M MOPS buffer (pH 3) and stained with 50 μg / ml 5,(6)-Carboxyfluorescein Diacetate (CFDA) (Invitrogen) in MOPS buffer for 1 h at 37°C in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... were cultured at 37°C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: HEK293 (ATCC CRL-1573) and HEK239A ΔGRK2/3/5/646 were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco 1196511) supplemented with 10% FBS (Hyclone SH30910.03) ...
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bioRxiv - Neuroscience 2023Quote: ... Zebrafish embryos were injured at 3 dpf and placed in 5 ug/mL acridine orange (ThermoFisher, Cat. No. A1301) diluted in egg water for 20 minutes ...
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bioRxiv - Synthetic Biology 2023Quote: ... 5 µL 40 µg/µL of each DSP was combined with 45 µL DNA coating solution (17250, ThermoFisher Scientific) and added to six wells of a medium-binding microplate (655076 ...
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bioRxiv - Synthetic Biology 2023Quote: ... 5 µL of total mRNA was converted to total cDNA using random hexamers following manufacturer’s instructions (Verso, Life Technologies).
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bioRxiv - Immunology 2023Quote: HEK 293T cells were maintained between 10% and 90% confluency at 37°C/5% CO2 in DMEM (Life Technologies) supplemented with 10% foetal bovine serum (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... 5 µg of each protein was injected on to a 2.1 × 30 mm Poros R2 column (Thermo Fisher Scientific) and resolved using the following rapid gradient ...
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bioRxiv - Cell Biology 2023Quote: ... 5×107 ES cells engineered with T7-dTAG-TAF1 were fixed with 1% formaldehyde (methanol-free, Thermo Fisher Scientific) for 10 min at 25C under constant gentle rotation ...
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bioRxiv - Neuroscience 2023Quote: ... the interscutularis muscle was exposed and labeled with 5 µg/ml Alexa 647-conjugated alpha-bungarotoxin (ThermoFisher Scientific, USA) for 10 minutes at room temperature to stain acetylcholine receptors as a guide for neuromuscular junctions ...
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bioRxiv - Biochemistry 2023Quote: ... coverslips were stained for 5 min with 1 μg/ml 300 nM 4′,6-diamidino-2-phenylindole (Life Technologies) to visualize nuclei ...
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bioRxiv - Biochemistry 2022Quote: ... After membrane permeabilization with 1% Triton X-100/D’PBS for 5 min cells were incubated for 30 min in the primary antibody aPMP70 (rabbit, 1:500, Invitrogen) in D’PBS supplemented with 1% BSA ...
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bioRxiv - Developmental Biology 2023Quote: ... transgenic hg13 larvae (mmachchg13/hg13 Tg(col2a1a:EGFP)) were staged and fixed at 5 DPF with 4% paraformaldehyde (Fisher Scientific). Larvae were dissected for genotyping and unbiased imaging was performed on the remaining head tissue ...
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bioRxiv - Cell Biology 2023Quote: ... or CDLK5 kinase domain at 4 μM in 10 mM HEPES-NaOH pH 7.4 and 500 mM NaCl was incubated with the inhibitors at different concentrations (12.5, 25, or 50 μM) in the presence of 5× SyPRO orange dye (Invitrogen). A Real-Time PCR Mx3005p machine (Stratagene ...
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bioRxiv - Developmental Biology 2023Quote: ... Larvae were maintained in embryo media at 28.5°C until 5 DPF and fixed in 4% paraformaldehyde (Fisher Scientific) at room temperature for 1 hour and stored in 1x PBS (Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was measured following 15-minute incubation with 5 nM SYTOX™ Red Dead Cell Stain (#S34859, Invitrogen) on ice as per manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... SPD-5 and GIP-1 cDNAs was amplified by PCR and cloned into the entry vector pDONR201 (Thermo Fisher). Thereafter ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 μg of total protein were loaded on NovexTM 10% Zymogram Plus (Gelatin) Protein Gels (Thermo Fisher Scientific: ZY00102BOX) using 2x non-reducing sample buffer (1M Tris-HCl pH 6,8 (Carl Roth ...
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bioRxiv - Cell Biology 2023Quote: ... 300-1500 ng DNA was incubated with 1-5 μl Lipofectamine 2000 in 150 μl Optimem (Thermo Fisher, 31985047) for 10 minutes at room temperature and then given to cells ...
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bioRxiv - Cancer Biology 2023Quote: ... On day 5 the culture medium was replaced with DMEM/F12 containing 1% N2 (v/v, Gibco, Cat#17502048), 1% GlutaMAX (v/v) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cat#30-2020) at 37 °C incubators supplied with 5% CO2 and passaged with Trypsin/EDTA (Gibco, Cat#15400054). For lentivirus production ...