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Citations for Thermo Fisher :
9401 - 9450 of 10000+ citations for TRAP 5 amide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... the pellet was air dried for 5 minutes prior to resuspension in nuclease-free TE buffer (Thermo Scientific #J75793).
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bioRxiv - Cell Biology 2022Quote: All HCT116 cell lines were cultured in humidified incubators at 37 °C and 5% CO2 using McCoy’s modified 5A medium (Gibco) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... A 300 μL solution of 5% formic acid and 0.2% trifluoroacetic acid (TFA) R2 50 μm Poros (Applied Biosystems) beads slurry in water was added to the gel pieces before returning the samples to the shaker for an additional three hours at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... cells were maintained in a humidified 37° C incubator with 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, MilliporeSigma) supplemented with 2 mM L-Glutamine (L-Glu, Gibco), 0.1 mM non-essential amino acids (NEAA ...
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bioRxiv - Cancer Biology 2022Quote: ... for 15 minutes at room temperature and then washed 3 times for 5 minutes each with pH 7.4 Phosphate Buffered Saline (PBS) (Gibco). Cells were then simultaneously permeabilized and blocked with a solution of 0.25% Surfact-Amps X-100 (Thermo Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... were diluted 1:5000 in 5 % BSA in TBST or in blocking buffer (Cat. no. 37538, Thermo Fisher Scientific) in PBS (1:1 ...
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bioRxiv - Biochemistry 2022Quote: ... Fluorescence measurements were attained by running the assay on the QuantStudio 5 real-time PCR system (Applied Biosystems™) at increasing temperatures from 25 to 95 °C at 0.1 °C s−1 increments ...
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bioRxiv - Biochemistry 2022Quote: ... 4,4-difluoro-5-(2-thienyl)-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid (BODIPY-C12, Invitrogen D3835), was dissolved in 100% ethanol and conjugated to 10% bovine serum albumin ...
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bioRxiv - Bioengineering 2022Quote: ... the EBs were transferred to StemPro-34 with rhVEGF (5□ng/ml) for another five days and then to DMEM high glucose (4.5□g/l, ThermoFisher) media with compaction factors (CHIR (1□uM ...
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bioRxiv - Cell Biology 2022Quote: ... cells were permeabilized with saponin (0.2%) for 5 min and incubated with DAPI (1 μg/mL) (ThermoFisher, catalog #: D1306) for 3 min to stain the nucleus ...
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bioRxiv - Developmental Biology 2022Quote: HeLa cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12) with Glutmax and 5% FCS (Gibco) and passaged every few days ...
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bioRxiv - Cell Biology 2022Quote: ... then resuspended in 100 μL staining buffer (10 mM HEPES, 140 mM NaCl, 2.5 mM CaCl2 pH 7.4, 5% Annexin-FITC (Invitrogen, A13199), 50 μg/mL propidium iodide (P1304MP ...
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bioRxiv - Developmental Biology 2022Quote: ... and 5 μl of the RT reaction was amplified in 25 μl using AccuPrime Pfx DNA polymerase (Thermo Fisher, 12344024 ...
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bioRxiv - Cell Biology 2022Quote: All cell lines were cultured at 37°C in 5% CO2 atmosphere in Dulbeccós modified medium (DMEM, Gibco TM, Thermofisher) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Genomics 2022Quote: ... Cells were cultured at 37 °C in a humidified incubator with 5% CO2 in high glucose Dulbecco’s Modified Eagle Medium (Cat. #41965039, Gibco) supplemented with 10 % bovine calf serum (Cat ...
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bioRxiv - Immunology 2022Quote: ... Cells then were washed twice with FACS buffer and stained for 5 minutes at 4°C with 0.5 mg/mL of 40,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher). PE and DAPI staining were measured with an iQue Screener Plus flow cytometer (Intellicyt ...
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bioRxiv - Genetics 2022Quote: Total RNA was purified from Cre-injected or uninjected embryos (5 to 10 per sample) with TRIzol reagent (ThermoFisher). For FACS-isolated mcherry+ cells (1.50-3.5×105 ...
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bioRxiv - Genetics 2022Quote: HeLa-JVM cells were maintained at 37°C and 5% CO2 in Dulbecco’s Modified Eagle’s Media (DMEM) (Life Technologies) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Evolutionary Biology 2022Quote: ... From these pre-cultures we used 5 μl to inoculate 384-well plates (Sigma-Aldrich, Nunc®, flat-bottom) containing 55 ul of SM media per well with inducer concentrations of 0.2% ...
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bioRxiv - Immunology 2022Quote: ... BCRJ 0234) cell lines were maintained at 37ºC and 5% CO2 in DMEM (1 g/L D-glucose, GIBCO) supplemented with 20% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... for 5 minutes and pellets were resuspended in Ammonium-Chloride-Potassium (ACK) lysing buffer (Thermo Fisher Scientific, Catalog # A1049201) to lyse RBC (erythrocytes removal performed at R.T ...
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bioRxiv - Neuroscience 2022Quote: Mouse neuroblastoma cells (N2a) were grown at 37C in a humidified incubator chamber under 5% CO2 in OptiMEM (Gibco), supplemented with 5% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... at room temperature with shaking (55 rpm) for 5 min and exposed to CL-Xposure Film (Thermo Fisher Scientific), which was then developed with an X-ray film processor (Konica Minolta SRX-101A).
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bioRxiv - Microbiology 2022Quote: qPCR assays were carried out using the QuantStudio 5 Real-Time PCR System (Thermo Fisher Scientific, San Jose, CA) and previously validated qPCR primers (3) ...
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bioRxiv - Microbiology 2022Quote: ... Tissue pieces from the antrum and corpus were placed in 50 ml conical tubes in 5 ml RPMI (Gibco) with 10% FBS (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... The culturing medium was aspired and replaced by seeding medium of Grace’s complete insect medium supplemented with 5% FBS (Gibco) and Graces’s unsupplemented insect medium ...
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bioRxiv - Microbiology 2022Quote: Human HEK-293T adherent cells were seeded at 37°C with 5% CO2 in DMEM + GlutaMAX™ (Gibco, Germany) containing 10% heat inactivated fetal calf serum (dFCS ...
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bioRxiv - Microbiology 2022Quote: HeLa (ATCC CCL-2) cells were grown at 37 ºC and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, GIBCO) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2022Quote: ... Messenger RNA (5 μg total RNA per sample) was enriched using a MICROBExpress bacterial mRNA purification kit (Thermo Fisher) and cDNA libraries containing unique barcodes generated from 100 ng mRNA using the Next UltraII Directional RNA Library Prep kit for Illumina (New England Biolabs) ...
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bioRxiv - Microbiology 2022Quote: ... and the crude nuclei) was incubated at 28 °C for 5 min in the presence of RNase inhibitor (Invitrogen). The RNA was then isolated by TRIzol reagent (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted from three distinct clonal populations of treatment and control cells (5 × 108 cells per replicate) using TRIzol reagent according to the manufacturer’s (Invitrogen) protocol ...
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bioRxiv - Plant Biology 2022Quote: ... pH 5.5 and 100 μM 3′,5′-dimethoxy-4′-hydroxyacetophenone (acetosyringone) (115540050; Acros Organics, dissolved in dimethyl sulfoxide (DMSO) (D8418 ...
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bioRxiv - Plant Biology 2022Quote: ... Each qPCR reaction contained 5 μl of the PowerUp™ SYBR™ Green PCR master mix (Applied Biosystems, USA), with 1 μl of each qPCR primer (Table S2 ...
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bioRxiv - Molecular Biology 2023Quote: ... the RNA pellet was allowed to dry at room temperature for a maximum of 5 minutes and then resuspended in 40μl DEPC-treated water (Invitrogen). The RNA quality was assessed on an Agilent Tapestation ...
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bioRxiv - Molecular Biology 2023Quote: ... Traces of genomic DNA were removed by treating ∼40 µg of total RNA with 5 U of DNase I (Fermentas) and 0.5 µL of Superase-In RNase Inhibitor (Ambion) in a reaction volume of 50 µL ...
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bioRxiv - Molecular Biology 2023Quote: ... and HT1080 cells were obtained from the American Type Culture Collection (ATCC) and maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 5% fetal bovine serum (Fisher Scientific), 5% bovine calf serum (Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... the dextran-conjugated Alexa680 solution (70 kDa, 0.1-0.15 ml at 5% W/V in PBS; Thermo Fisher Scientific) was retro-orbitally injected into the bloodstream ...
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bioRxiv - Neuroscience 2022Quote: ... cells were again centrifuged and re-suspended for 5 min at room temperature in Dulbecco’s phosphate-buffered saline (14190-094, Invitrogen) supplemented with 5 mg/ml bovine serum albumin ...
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bioRxiv - Microbiology 2022Quote: ... or BA.4/5 spike protein (4 μg/mL) was immobilized on 96-well Maxisorp ELISA plates (Thermo Fisher) overnight at 4°C in coating buffer (1X PBS supplemented with 0.05% Tween-20 ...
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bioRxiv - Molecular Biology 2023Quote: ... and a 7900HT Fast Real Time PCR System or a Quant Studio 5 Real Time PCR System (Applied Biosystems) with the primers listed in Supplemental Table S1 ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were rinsed 3 x 5 min and cell nuclei were labeled by DNA staining using Hoechst (Life Technologies) for 30 min at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Cultures were maintained in a humified incubator at 37 °C and 5% CO2 in Neurobasal medium (Gibco™ 21103049) supplemented with B27 (Gibco™ 17504044) ...
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bioRxiv - Microbiology 2022Quote: ... One ml of fd or Pf4 phage (5 mg/ml) was incubated with 100 µg A488 fluorescent dye (ThermoFisher) for 1 hour at room temperature (RT ...
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bioRxiv - Biochemistry 2022Quote: ... cells were maintained in a humidified 37° C incubator with 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, MilliporeSigma) supplemented with 2 mM L-Glutamine (L-Glu, Gibco), 0.1 mM non-essential amino acids (NEAA ...
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bioRxiv - Physiology 2022Quote: ... Cells were grown in DMEM-F-12 (D6421) supplemented with 5% FBS (GIBCO/Thermo Fisher Scientific, Grand Island, NY) and maintained at 37°C in 5% CO2 ...
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bioRxiv - Neuroscience 2022Quote: ... ACC 57) were cultured at 37°C and 5% CO2 in high glucose Dulbecco’s Modified Eagle Medium (DMEM; Gibco) supplemented with 10% fetal calf serum (Linaris) ...
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bioRxiv - Neuroscience 2022Quote: ... Eyes were intravitreally injected using a glass micropipette with 1-5 μl of 0.2% cholera toxin subunit B (CTB) conjugated to Alexa Fluor 488 or 555 (Invitrogen) diluted in 1% DMSO ...
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bioRxiv - Neuroscience 2022Quote: ... NSCs were seeded at a density of 5 × 104 cells/cm2 and precoated with Geltrex (A1413301, Thermo Fisher Scientific) in NSC expansion medium ...
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bioRxiv - Neuroscience 2022Quote: ... The cells were loaded with 5 µM calcium-sensitive fluorescent dye Fluo-4AM (Direct Calcium Assay Kit, Invitrogen, USA) for 30 min at 37° C and washed for 15 min or 30 min at 37° C and then for 5 min at RT in basic solution (BS ...
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bioRxiv - Molecular Biology 2022Quote: ... 15 ug of psPAX2 and 5 ug pMD.G plasmid were vortexed with 40 ul P3000 reagent in 1.5 ml OptiMEM (ThermoFisher, 31985070). Then 40 ul Lipofectamine was added to 1.5 ml OptiMEM and briefly vortexed ...