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Citations for Thermo Fisher :
9751 - 9800 of 10000+ citations for TRAP 5 amide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... The remaining part of embryo was treated with 5 μL Cells-to-cDNA™ II Cell Lysis Buffer (Invitrogen), and then reverse-transcribed into cDNA with the HiScript II Q Select RT Kit (Vazyme).
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bioRxiv - Synthetic Biology 2024Quote: Kinetic constants of SrIRED for cyclohexanone were determined in 100 mM Tris pH 8.0 with 0.5 mM NADPH and varying concentrations of cyclohexanone (0.06 mM - 35 mM) in transparent Nunc 96-well plates (ThermoFisher) and by monitoring the depletion of NADPH concentration at λ of 340nm (Spectramax 190 ...
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bioRxiv - Biochemistry 2024Quote: ... Reaction mixtures were adjusted to a total volume of 5 μl (2 μl solution A; 1,5 μl solution B; 0,3 μl RiboLock (40 U/μl, Thermo Scientific); 0,2 μl D-Luciferin (10 mM ...
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bioRxiv - Immunology 2024Quote: ... the cells were seeded at 5 × 104 cells per well in U-bottom 96-well plates (Fisher Scientific; #163320). Two dilutions of antibody ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was placed into a hybridization bottle and was prehybridized with 5 ml ULTRAhyb Ultrasensitive Hybridization Buffer (Invitrogen) at 42°C for 45 minutes in a hybridization oven ...
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bioRxiv - Microbiology 2024Quote: ... 100 µl of culture supernatant was collected and treated with 2 µl (5 U) DNase solution I (ThermoFisher Scientific) at 37°C for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... 5 × 105 cells/ml were plated in three 50 mm non-treated tissue-culture dishes (122-17, Thermo Scientific). For control ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were transfected with 2-5 µg of the indicated plasmid using Lipofectamine 3000 as per manufacturer’s instructions (ThermoFisher). Transfection medium was exchanged to fresh medium and cells were moved to 10 cm plates 4-6 hours post-transfection ...
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bioRxiv - Physiology 2024Quote: Platelet intracellular Ca2+ was measured as described previously using 5 µM Fluo-4 fluorescence dye (Invitrogen, Waltham, Massachusetts, USA).20 1 x 105 washed platelets were labeled with Fluo-4 for 30 min at room temperature (RT) ...
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bioRxiv - Physiology 2024Quote: 5 μL hemolymph of each biological replicate was transferred into pre-chilled siliconized glass vials (Fisher Scientific 03-395G) on ice with a siliconized glass pipette and supplemented with 150 μL of chilled PBS from the same tip ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... were maintained in a water jacketed 5 % CO2 incubator and passaged every 2-3 days using trypsin/EDTA (Gibco). 1E6 cells were seeded into 6 well plates ...
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bioRxiv - Immunology 2024Quote: 2×106 THP-1 cells were centrifuged for 5 min at 1200 rpm and washed in 1X PBS (Gibco). They were then transfected by nucleofection with 3 μg of plasmid DNA in 100 μl of Cell Line Nucleofector Solution V (Lonza ...
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bioRxiv - Genetics 2024Quote: ... The paraffin blocks were cut into 5-um-thick sections using a Microm HM 325 microtome (Thermo Scientific, USA) and allowed to dry on glass slides overnight at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... were cultured in a humidified incubator (5 % CO2, 37 °C) on 0.1 % gelatin-coated cell culture dishes in ESLIF medium (GMEM (Gibco) containing 10 % fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2024Quote: ... All cell lines were maintained in a humidified incubator at 37°C with 5% CO2 in DMEM (GIBCO-11995) supplemented with 10% heat-inactivated fetal bovine serum (#10082147 ...
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bioRxiv - Genomics 2024Quote: ... Real-time quantitative PCR reactions were performed using SYBR Green reagent and analyzed using QuantStudio 5 System (Applied Biosystems). HPRT and B2M were used as housekeeping genes ...
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bioRxiv - Genomics 2024Quote: 129S1/SvImJ / C57BL/6 mESCs24 were cultured in a humidified incubator (5 % CO2, 37 °C) on 0.1 % gelatin-coated cell culture dishes in ESLIF medium (GMEM (Gibco) containing 10 % fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: Passaging of ROBO1+ sorted Rounded/CBC cultures every 4-5 days is best done by incubating cells with 0.25% trypsin-EDTA (Gibco) for 1-2 min at RT/37°C ...
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bioRxiv - Physiology 2024Quote: ... the peptides were concentrated on an Accalaim μ-Precolumn (0.5 mm × 3 mm, particle size 5 μm; Thermo Scientific) in the isocratic mode at a 10 μL/min flow for 5 min in the mobile phase C (2% acetonitrile ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 5-LOX was stained with Alexa Fluor™ 555 goat anti-mouse IgG (1:500, # A21422, Lot: 10143952; Invitrogen), and perilipin-2 was stained with Alexa Fluor™ 488 goat anti-rabbit IgG (1:500 ...
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bioRxiv - Molecular Biology 2024Quote: ... Glycopeptides were trapped on a C18 cartridge column (Acclaim PepMap100 C18, 0.3 mm I.D. × 5 mm; Thermo Fisher Scientific) and separated on a C18 tip column (75 µm I.D ...
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bioRxiv - Physiology 2024Quote: FAP proliferation was detected using 5-ethynyl-2’-deoxyuridine (EdU)/click-it assay (cat. no.: C10337 and C10340, Invitrogen). Experiments were carried out in ECM coated 96-well half-area plates ...
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bioRxiv - Microbiology 2024Quote: The samples prepared for light microscopy were sectioned at 5 μm with an HM 325 rotary microtome (ThermoFisher-Scientific) and stained them with hematoxylin and eosin ...
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bioRxiv - Neuroscience 2024Quote: ... once in 0.5% BSA with 0.05% Tween20 in PBS for 10 minutes and incubated with secondary antibodies and Hoechst33342 (H1399, ThermoFisher) for 1 hour at RT in the dark ...
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bioRxiv - Cancer Biology 2024Quote: ... GCT cell lines were routinely maintained at 37°C with 5% CO2 in complete medium consisting of Dulbecco’s modified Eagle medium: nutrient mixture F-12 (DMEM/F12) (Gibco) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Genomics 2024Quote: ... human female) cells were cultured in a 37°C 5% CO2 and 20% O2 humidified incubator with DMEM (Gibco) supplemented with 10% heat-inactivated FBS (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were washed three times (in 0.1% Tween-20 in PBS (PBST) before incubation with secondary antibodies (5 μg/ml; Invitrogen) in 5% BSA in PBS for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... qPCR master mix was prepared by mixing 5 µL SYBR Green PCR Master Mix (Thermo Fisher Scientific, Cat# A25780) with 0.5 µL primer mix (forward and reverse 10uM ...
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bioRxiv - Cancer Biology 2024Quote: OSCC cells were collected and suspended in PBS containing 5 μM C11-BODIPY 581/591 (Thermo Fisher Scientific, #D3861) in the dark at 37°C for 30 min and washed 3 times with PBS ...
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bioRxiv - Cell Biology 2024Quote: ... An overnight incubation with specific antibodies (P16, IL-6 or phospho-H2AX (S139) and cTNT (Invitrogen, #MA-5-12960)) was followed by 2 hours of incubation with secondary antibody conjugated with Alexa Fluor 488 ...
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bioRxiv - Cell Biology 2024Quote: ... AML12 cells were cultured at 37°C in 5% CO2 in DMEM/Ham’s F12 50/50 media (Fisher Scientific) with 1X Insulin-Transferrin-Selenium (ThermoFisher) ...
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bioRxiv - Cell Biology 2024Quote: ... HEK 293T and MEF cells were cultured at 37°C with 5% CO2 in DMEM/F12 medium (Thermo Fisher Scientific – Life technologies #LTI 11039-047 ...
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bioRxiv - Immunology 2024Quote: ... lymph node suspensions at 107 cells per mL in PBS 0.5%FCS were labelled with 5 µM CTV (Invitrogen) for 20 min at 37°C before reaction was quenched and cells washed with cold medium ...
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bioRxiv - Genomics 2024Quote: ... cells were cultured in a 37°C 5% CO2 and 20% O2 humidified incubator with Dulbecco’s Modified Eagles Medium (DMEM, Gibco) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: HEK293t cells were detached and transferred to 75-mm flow tubes and exposed to 5 μM MitoSOX (Invitrogen, M36008) or 10 μM DCFH-DA (Beyotime ...
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bioRxiv - Cancer Biology 2024Quote: ... B16-F10-luc cells were selected at 37 °C and 5 % CO2 in a DMEM medium supplemented with 0.2 mg/mL zeocin (Invitrogen), 10 % FBS ...
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bioRxiv - Biochemistry 2024Quote: ... 7.5 μg of the substrate and 4.5 μg of HA-Ubiquitin were transfected with 30 μL Lipofectamine 2000 (Invitrogen) in Opti-MEM (Gibco) ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were washed 2× with PBSTw for 5 min each and mounted in VectaShield Antifade (Fisher Scientific, Cat# NC9532821) mounting solution and stored at 4 ℃.
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bioRxiv - Molecular Biology 2024Quote: ... using a pre-column for sample loading (Acclaim PepMap C18, 2 cm × 0.1 mm, 5 μm, Thermo-Fisher Scientific), and a C18 analytical column (Acclaim PepMap C18 ...
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bioRxiv - Bioengineering 2024Quote: Protein samples were diluted in PBS to 5 μM and mixed with 5000× SYPRO Orange Protein Gel Stain (Invitrogen) to final 1.25x concentration ...
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bioRxiv - Cell Biology 2024Quote: ... cells were stimulated with VEGF (20 ng/ml) for 30 min and then incubated with 5 μM CellRox (Invitrogen) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lines were grown at 37°C/5% CO2 in Dulbecco’s Modified Eagle Medium (Thermo Fisher Scientific, Cat #11965118) supplemented with 10% fetal bovine serum (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: BCP-ALL cell lines were maintained at 37 °C and 5% CO2 in RPMI GlutaMAX (Thermo Fisher Scientific, #61870036) with 20% fetal bovine serum (Biowest ...
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bioRxiv - Microbiology 2024Quote: ... and remaining tissue incubated in 1X HBSS - without Ca2+ and Mg2+ containing 5 mM EDTA (Fisher Scientific; cat# AM9260G) and 1 mM DL-Dithiothreitol (DTT ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Quantitative PCR was performed with RealQ Plus 2x Master Mix Green (Ampliqon, #A324402) using a QuantStudio 5 (Applied Biosystems) instrument ...
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bioRxiv - Molecular Biology 2024Quote: ... Biotin conjugated EdU-labeled DNA was then pulled down using 5 μL of Dynabeads M-280 streptavidin (Invitrogen, 11205D). Beads were pre-washed three times with 1x Tween buffer (5 mM Tris HCl pH = 8 ...
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bioRxiv - Biochemistry 2024Quote: ... single colonies for each variant were inoculated into a started culture of 5 mL Terrific Broth media (Thermo Scientific) containing 100 μg/mL ampicillin and grown at 37°C overnight in a 180 rpm shaking incubator ...
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bioRxiv - Cell Biology 2024Quote: tdTomato-tractin MEFs were cultured at 37°C in a 5% CO2 incubator in Dulbecco’s modified Eagle medium (Biological Industries, BI) supplemented with 10% fetal bovine serum (FBS, Gibco) and penicillin-streptomycin (P/S ...
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bioRxiv - Cell Biology 2024Quote: ... tilted images series (+60°) were acquired with a Talos L120C TEM using Tomography 5 software (FEI, Thermo Fisher Scientific). Tilted images were aligned ...
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bioRxiv - Cell Biology 2024Quote: ... HEK293 cells were incubated in 5% CO2 at 37 °C and passaged every 72 hr with trypsin (Gibco, #25300054) not allowing them to reach confluency.