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Citations for Thermo Fisher :
9301 - 9350 of 10000+ citations for TRAP 5 amide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Between 5 and 10ng of cDNA were used in a 4ul reaction using POWEUP SYBR qPCR mix (Life Technologies). All primer-sets were run in technical triplicates ...
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bioRxiv - Genomics 2021Quote: ... centrifuged at 500xg for 5 minutes at 4°C using a swinging bucket rotor (Sorvall Legend RT, Thermo Fisher), and then pellets were washed with an additional 1 mL cold lysis buffer and incubated on ice for an additional 5 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... Gibco, 11965092; 10% FBS, heat-treated at 56C for 30 minutes, Gibco, 26140079; 5% penicillin-streptomycin 10,000U/mL, Gibco 15140122 ...
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bioRxiv - Biochemistry 2021Quote: Synthetic genes encoding for human merlin FERM (22-312) and radixin FERM (5-295) domains were commercially synthesized (ThermoFisher) and cloned into pETM33 using NcoI and EcoRI restriction sites ...
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bioRxiv - Molecular Biology 2021Quote: ... Both 5 mL and 50 mL tubes were filled with 99.8% molecular grade ethanol (Fisher BioReagents™, Fisher Scientific) and stored in the dark on dry ice during shipment to the University of Otago’s PCR-free eDNA facilities ...
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bioRxiv - Microbiology 2022Quote: ... Coverslips were washed with 5% FBS and stained with AlexaFluor-488-conjugated goat anti-rat IgG (Invitrogen, 1:500) for 1 h at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were boiled at 95°C for 5 minutes and 15–30μl was separated on Tris-Glycine SDS-PAGE gels (ThermoFisher) followed by Western blotting onto nitrocellulose membranes (ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were seeded onto 5 flasks coated with gelatin 5cm2 in F12 medium (Life Technologies, Gibco® 31765-027) with 20% FBS (Dutscher ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were seeded onto 5 flasks coated with gelatin 5cm2 in F12 medium (Life Technologies, Gibco® 31765-027) with 20% FBS (Dutscher ...
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bioRxiv - Cancer Biology 2020Quote: ... Human primary AML cell were grown at 37°C with 5% CO2 in Iscove’s Modified Dulbecco’s Media with Glutamax (IMDM, Gibco, Life Technologies) supplemented with 20% fetal calf serum (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 0.05 mg/mL bovine pituitary extract and 5 ng/mL human recombinant epidermal growth factor (Gibco, USA). Cells were maintained in a humidified incubator at 37°C with 5% CO2 level ...
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bioRxiv - Plant Biology 2021Quote: ... both fragments were independently amplified by PCR and cloned into the intermediate vectors pENTRY 5’ TOPO and pCR8 (Invitrogen), respectively ...
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bioRxiv - Physiology 2021Quote: ... were plated in plates 1 day before transfection with a total of 2 μg of DNA (1 μg mouse pCMV6-Tmem27-Flag and 1 μg empty vector or pCDNA3.1-BACE2-myc) and 5 μL Lipofectamine 2000 (Invitrogen).
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bioRxiv - Cancer Biology 2021Quote: ... which were boiled for 5 min at 95°C and then separated on 4-12% polyacrylamide gradient gels (Invitrogen). After blotting ...
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bioRxiv - Biophysics 2021Quote: Superparamagnetic polystyrene beads with a diameter of 1.08 μm (coefficient of variation < 5%, Dynabeads MyOne™ Tosylactivated, 65501, Invitrogen) were functionalized via an amine coupling reaction with 20 μg of anti-digoxigenin Fab fragments (Invitrogen ...
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Bidirectional neuronal migration coordinates retinal morphogenesis by preventing spatial competitionbioRxiv - Developmental Biology 2021Quote: ... iPSCs and developing organoids were cultured at 37°C and 5% CO2 in a humidified incubator (Thermo Fisher Scientific).
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Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cellsbioRxiv - Developmental Biology 2020Quote: ... Tissues were centrifuged at 200 g for 5 min and washed with Hank’s Balanced Salt Solution (Thermo Fisher Scientific). Tissues were then digested with 0.25% trypsin/EDTA and DNase I for 8 min at 37 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... Precipitated RNAs were quantified using Taqman miRNA assays with custom primers for detection of 5’-tRFCys (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2021Quote: ... LDHC expression was quantified by specific 5′FAM-3′MGB Taqman gene expression primer/probe sets (Hs00255650_m1, Applied Biosystems). MAP1B expression was quantified using primers for SYBR-based qPCR (F ...
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bioRxiv - Cancer Biology 2020Quote: ... samples were blocked in PBS +5% BSA +0.3% Triton X-100 in PBS and stained using an AlexaFluor488-labeled ZO-1 (#339188, Invitrogen) or ROR2 (#FAB20641G ...
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bioRxiv - Cell Biology 2019Quote: ... (1.5 μl microsomes in 48.5 μl PBS) in the absence or presence of 0.1% Triton X-100 (Thermo Fisher). The solution was added into a CELLSTAR™ 96 Well Polystyrene Flat Bottom Cell Culture Microplate (Greiner Bio-One ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were pelleted at 300 x g for 5 minutes and resuspended in freezing medium (fetal bovine serum (Gibco), 10% v/v DMSO) ...
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bioRxiv - Cell Biology 2020Quote: ... Melanoma cell line 501mel was grown in 5% CO2 at 37°C in RPMI w/o HEPES (Gibco, Invitrogen) supplemented with 10% FCS and Gentamycin ...
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bioRxiv - Cell Biology 2020Quote: ... All cells were maintained in 1:1 Ham’s F12 Dulbecco’s modified Eagle’s medium (FDV) containing 5% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 5ml/500ml 100X penicillin-streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... the pDyn3 plasmid containing the human dynein genes or the pFastBac plasmid containing full-length Lis1 was transformed into DH10EmBacY chemically competent cells with heat shock at 42°C for 15 seconds followed by incubation at 37°C for 5 hours in S.O.C media (Thermofisher scientific). The cells were then plated on LB-agar plates containing kanamycin (50 μg/ml) ...
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bioRxiv - Genetics 2021Quote: ... in PBS for 1 minute (min), centrifuged (600 g, 5 min) and then incubated in Colagenase (0.5 mg/ml) (Gibco) in RPMI medium (Lonza ...
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bioRxiv - Genetics 2020Quote: ... qPCR were carried out on the generated cDNA (diluted at 1/5) using PowerUp SYBR master mix (Thermofisher Scientific) in a final volume of 10 μl and 40 cycles of amplification on QuantStudio3 Real-Time PCR System (ThermoFisher) ...
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bioRxiv - Genomics 2021Quote: HEK-293 cells (ATCC CRL-1573) were grown at 37°C in 5% CO2 in Dulbecco’s Modified Eagle Medium (Gibco) supplemented with 10% FBS (Omega Scientific ...
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bioRxiv - Genomics 2020Quote: The eluted cDNA:mRNA hybrids (15 μl) were combined with second strand mix [2 μl 5× First Strand Buffer (Invitrogen), 2.31 μl Second Strand Buffer (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... except that BME vitamin solution 100x (Bioconcept 5-20K00-H) was used instead of vitamin mix 100x (Invitrogen 010144) and 1% Pen/Strep stock solution (Bioconcept 4-01F00-H ...
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bioRxiv - Microbiology 2021Quote: Green monkey kidney fibroblast-like Cos7 cells were cultured at 37 °C with 5% atmospheric CO2 in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... CRL-1586) were maintained at 37°C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Hyclone or GIBCO) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then permeabilized using PBS with 0.2% Triton X-100 for 15 min and blocked with 5% FBS (Gibco), followed by treatment with the primary anti-JEV prM antibody (4 ...
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bioRxiv - Immunology 2020Quote: Spleens were removed from each mouse and placed in 5 mL of sterile medium of RPMI (Gibco Cat # 22400105), HEPES (Hyclone Cat# SH30237.01) ...
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bioRxiv - Microbiology 2021Quote: ... approximately 500 10-days old flies were squashed for 5 minutes in 10ml Schneider’s Insect Medium (Thermo Fisher Scientific) using glass beads ...
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bioRxiv - Immunology 2020Quote: ... Pen/Strep and 5% IgG-depleted FBS (CCU, The Pirbright Institute or GIBCO, ultra-low IgG, ThermoFisher Scientific, UK).
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bioRxiv - Immunology 2020Quote: ... live CD3+CD8+) were sorted from a C57BL/6Thy1.1 mouse as responder cells and labeled with 5 μM CellTrace Violet (Invitrogen). Whole splenocytes from 6-8 week-old ...
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bioRxiv - Immunology 2020Quote: ... All cell lines were cultured in a humidified 37°C incubator (5% CO2) in Dulbecco’s Modified Eagle Medium (Gibco) supplemented with 10% Fetal Bovine Serum and 1% Penicillin/Streptomycin ...
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bioRxiv - Molecular Biology 2021Quote: ... then co-transfected with 0.25 µg of pcDNA™5/FRT/TO and 2.25 µg of pOG44 Flp recombinase expression plasmid (Invitrogen) using Lipofectamine LTX with Plus Reagent (Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: ... and Il36A_rev (5′-CAGTTCTTGGGTCAGAATGAGTG-3′) and subsequent cloning into pJET1.2/blunt vector as described by the manufacturer (ThermoFisher Scientific). The DNA fragment encoding mouse C/EBPβ residues 221–296 (bZIP domain ...
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bioRxiv - Microbiology 2020Quote: Samples consisting of either solubilized membranes or purified membrane complexes were mixed with 5% G-250 sample additive (Invitrogen), to a final concentration of ~0.2% ...
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bioRxiv - Microbiology 2020Quote: ... 2002) were maintained at 37 °C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Fisher Scientific, cat. #11995065) supplemented with 0.1 mM nonessential amino acids (NEAA ...
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bioRxiv - Microbiology 2020Quote: ... African green monkey kidney epithelial Vero-E6 cells (ATCC, CRL-1586) were maintained at 37°C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Microbiology 2020Quote: Spinner-adapted L cells were grown in suspension culture in Joklik’s minimum essential medium (US Biological) supplemented to contain 5% fetal bovine serum (FBS) (Gibco), 2 mM L-glutamine (Corning) ...
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bioRxiv - Microbiology 2020Quote: ... Huh-7 .5 and BHK-21 cells were cultured in Dulbecco’s minimal essential medium (DMEM) (Gibco /Thermo Fisher Scientific) supplemented with 2mM L-glutamine (Gibco /Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Huh-7 .5 and BHK-21 cells were cultured in Dulbecco’s minimal essential medium (DMEM) (Gibco /Thermo Fisher Scientific) supplemented with 2mM L-glutamine (Gibco /Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: Human cervical epithelial adenocarcinoma HeLa cells were cultured at 37° C with 5% atmospheric CO2 in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Blocks were sectioned to 5 μm thickness using a Leica microtome (RM2145) and applied to Ultrastick slides (Thermo Scientific). Sections were stored at room temperature until CLASI-FISH was performed.
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bioRxiv - Immunology 2021Quote: B16-F10 cells (ATCC, CRL-6475) were purchased and cultured at 37°C in 5% CO2 in DMEM (Invitrogen), 10% FCS (Benchmark) ...
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bioRxiv - Immunology 2020Quote: mRNA from 2-5 x 104 sorted cells was captured with 12 μl of Dynabeads oligo(dT) (Life Technologies), washed ...