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Citations for Thermo Fisher :
901 - 950 of 10000+ citations for C Reactive Protein Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ROS assay was performed using a Total Reactive Oxygen Species Assay Kit 520nM (Cat. Nr. 88-5930-74, Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The carboxyl groups on the bead surfaces were functionalized with amine-reactive groups via N- ethyl-N’-(3-(dimethylamino)propyl)carbodiimide (EDC) and sulfo-N-hydroxysuccinimide (sulfo-NHS, Thermo Fisher) crosslinking for 20 minutes at 4°C ...
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bioRxiv - Cell Biology 2024Quote: Cells were subjected to MG132 treatment for 16 h and generation of cellular reactive oxygen species (ROS) was measured using CellROXTM Deep Red Reagent (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2019Quote: ... Proteins (20µg) were heated at 95°C with β-mercaptoethanol and then separated by SDS PAGE (Invitrogen). Nitrocellulose membranes were blocked in 5% nonfat milk in PBST for 1 hr and then incubated in primary antibody overnight at 4ºC ...
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bioRxiv - Biochemistry 2019Quote: ... 4°C and lysed using 400 µL B-PER bacterial protein extraction reagent (Thermo Scientific, MA, USA) according to the instructions of the manufacturer ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated at 4°C for 30-60 minutes with Protein A Dynabeads (Thermo Fisher Scientific). The beads were washed five times in wash buffer (20 mM Tris ...
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bioRxiv - Neuroscience 2020Quote: ... 4 °C) and the protein concentration of the supernatant was determined using a BCA assay (ThermoFisher 23225). Samples were denatured (70% sample ...
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bioRxiv - Microbiology 2022Quote: ... Protein lysates were boiled at 95 °C for 5 mins in 6x SDS sample buffer (Thermo Scientific). Proteins were separated on 4-12% Bis-Tris SDS-PAGE gels (Thermo Fisher ...
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bioRxiv - Developmental Biology 2022Quote: ... at 95°C for 5 min and separated using NuPAGE 4%–12% Bis-Tris Protein Gel (Invitrogen) in NuPAGE MES SDS Running Buffer (Invitrogen) ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein was denatured at 70 °C for 10 min with LDS Sample Buffer (Invitrogen, Cat#: NP0007) and Sample Reducing Agent (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: The purified JEV structural proteins (C, M, E, ED III) from the S2 insect expression system (Invitrogen) were quantified by using the bicinchoninic acid (BCA ...
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bioRxiv - Biochemistry 2021Quote: ... 0.03% NP40) and proteins were eluted by incubation in 1x LDS-sample buffer (Thermofisher, c/n NP0007) at 65°C for 10 minutes ...
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bioRxiv - Microbiology 2021Quote: ... sheared chromatin was precleared for 20 min at 4’C using Protein G Dynabeads (Thermo Fisher Scientific). Immunoprecipitations were performed using fresh beads and anti-Histone H3 acetyl K27 (H3K27Ac ...
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bioRxiv - Molecular Biology 2022Quote: ... and stored at −30°C before separation on 4-12% NuPAGE Bis-Tris protein gels (ThermoFisher Scientific).
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bioRxiv - Immunology 2022Quote: Recombinant tri-S protein was heat-denatured at 100°C for 3 min in loading buffer (Invitrogen) containing 1X sample reducing agent (Invitrogen) ...
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bioRxiv - Molecular Biology 2019Quote: ... Immunoprecipitation was performed at 4°C for 3 hr with 50 µl protein G dynabeads (Thermo Fisher). Beads were washed five times with ice-cold Wash buffer 1 (50 mM Hepes-KOH (pH7.6) ...
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bioRxiv - Microbiology 2020Quote: Proteins with C-terminal biotin tags were attached to streptavidin-coated dynabeads (Dynabead M-280 Streptavidin, Invitrogen) following standard procedures ...
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bioRxiv - Genetics 2020Quote: ... Proteins were denatured for 10 min at 70°C in NuPAGE LDS Sample Buffer (4X) (Invitrogen; NP0007) and NuPAGE Sample Reducing Agent (10X ...
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bioRxiv - Physiology 2021Quote: ... The total protein was inoculated with anti-HA magnetic beads at 4 C (Thermo Scientific, Rockford, IL) overnight ...
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bioRxiv - Immunology 2022Quote: ... STAT1 was immunoprecipitated by incubating for five hours at 4°C with Protein-G DynaBeads (Invitrogen #10004D). DNA bound to STAT1 complexes was extracted using 1% SDS and 0.6 mg/ml of Proteinase K (New England Biolabs #P81075 ...
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bioRxiv - Microbiology 2023Quote: ... Protein samples were denatured at 90 °C for 10 min with 10× reducing agent (Thermo Fisher Scientific) before gel electrophoresis ...
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bioRxiv - Neuroscience 2024Quote: ... 4°C at 20817 x g and protein concentrations were determined by BCA assay (Thermo Fisher, 23225). Cell lysates (10 μg for HEK293E cells or 15 μg for MEFs ...
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bioRxiv - Biophysics 2024Quote: ... precleared and immunoprecipitated overnight at 4 °C with 50 μL of GFP-conjugated Dynabeads Protein A (Invitrogen). Beads had previously been conjugated with 10 μL anti-GFP antibody (Invitrogen ...
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bioRxiv - Biophysics 2024Quote: ... precleared and immunoprecipitated overnight at 4 °C with 50 μL of GFP-conjugated Dynabeads Protein A (Invitrogen). Beads had previously been conjugated with 5 μL anti-GFP antibody (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... before being incubated overnight at 4 degrees C with the antibodies bound to Protein A Dynabeads (Invitrogen). Samples were washed for 3 minutes each at 4 degrees C with low salt wash (0.01% SDS ...
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bioRxiv - Immunology 2023Quote: ... sheared chromatin was precleared for 20 min at 4°C using Protein G Dynabeads (Thermo Fisher Scientific). Immunoprecipitations were performed using fresh beads and anti-Histone H3 acetyl K9 (H3K9Ac ...
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bioRxiv - Biochemistry 2022Quote: ... Target protein was coated overnight (O/N) at 4 °C on MaxiSorp™ 96-well plates (Nunc). ELISA-blocking buffer (PBS containing 0.5% (w/v ...
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bioRxiv - Developmental Biology 2023Quote: ... at 95°C for 5 min and separated using NuPAGE 4%–12% Bis-Tris Protein Gel (Invitrogen) in NuPAGE MES SDS Running Buffer (Invitrogen) ...
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bioRxiv - Plant Biology 2023Quote: ... The protein samples were denatured at 65 °C in the 1X LDS sample buffer (Invitrogen, Waltham, MA) with 2.5% β-mercaptoethanol for 5 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... The obtained supernatants (cell lysates) were incubated at 4°C with Dynabeads Protein G (Thermo Fisher Scientific) pre-bound with antibodies ...
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bioRxiv - Biochemistry 2024Quote: ... 4°C) and the protein concentration determined by a BCA assay (Pierce BCA Kit, Thermo Fisher Scientific). The human protein extracts were further processed to peptides in the same manner as described above for bacterial protein extracts.
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bioRxiv - Cell Biology 2023Quote: ... Samples were stored at −80 °C or directly used for the quantification of total protein concentrations with the Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA, United States). Thereafter ...
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bioRxiv - Genomics 2020Quote: ... Peptides were resuspended in 0.1% TFA 2% ACN and quantified using Pierce™ Quantitative Fluorometric Peptide Assay (Thermo Fisher Scientific). Equal portions of all samples were mixed together to make a reference sample to be run multiple times for chromatogram library runs.
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bioRxiv - Cancer Biology 2022Quote: ... samples were resuspended in 0.1% FA and peptides were quantified by Pierce™ Quantitative Fluorometric Peptide Assay (Thermo Fisher Scientific). 500 ng of peptides of each sample were analyzed by Liquid Chromatography-Mass Spectrometry (LC-MS).
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bioRxiv - Microbiology 2022Quote: Peptides were fractionated into eight fractions using the high pH reversed-phase peptide fractionation kit (Pierce™ Thermo Fisher Scientific) according to the manufacturer’s instructions and using self-made columns as previously described48 ...
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bioRxiv - Cancer Biology 2021Quote: Two-dimensional fractionation—Peptides were fractionated using a Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific, 84868) according to the manufacturer’s instructions with slight modifications ...
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bioRxiv - Physiology 2021Quote: ... Peptide concentration was measured with a colorimetric assay (Pierce Quantitative Colorimetric Peptide Assay kit, Thermo Fisher Scientific, Rockford, IL, USA).
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bioRxiv - Immunology 2020Quote: ... the HLA-A*02:01-restricted KRASwt (KLVVVGAGGV) and KRASG12D (KLVVVGADGV) aa5-14 peptides were synthesized (Peptide 2.0) and analyzed on an Orbitrap Fusion (Thermo Scientific) mass spectrometer (MS ...
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bioRxiv - Immunology 2022Quote: ... The Hc gene with a N-terminal gp67 signal peptide and Lc gene with a N-terminal honeybee melittin signal peptide were subcloned into pFastBac Dual vector (Invitrogen) containing two multiple cloning sites ...
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bioRxiv - Cancer Biology 2024Quote: ... The concentration of tryptic peptides was measured using the Pierce™ Quantitative Peptide Assays kit (Thermo Fisher Scientific cat# 23275), and 25 µg of peptides were separated on a home-made reverse-phase C18 column in a pipet tip as previously (38) ...
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bioRxiv - Immunology 2022Quote: ... MBP peptide NPVVHFFKNIVTPR and influenza hemagglutinin PRFVKQNTLRLAT peptide were labeled with Alexa Fluor 488 (Alexa488) tetrafluorophenyl ester (Invitrogen, Carlsbad, CA) and used as probe peptides for DP4.1 ...
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bioRxiv - Biochemistry 2023Quote: ... Tryptic peptide mixtures including the stable-isotope labelled standard peptides were analysed on a nano-HPLC (Easy nLC, Thermo Scientific) coupled to an Orbitrap mass spectrometer (LTQ-Orbitrap ...
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bioRxiv - Biochemistry 2023Quote: ... HM and CM peptides resulting from the previous step were desalted using peptide desalting spin columns (Thermofisher Scientific, Brisbane, Australia), according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... solubility of 19F peptide library was estimated by fluorescence spectroscopy using Pierce Quantitative Fluorometric Peptide Assay (Thermo Fisher Scientific, USA), and 0.5 mg/ml solution in DMSO was used as a standard ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... The mixture of TMT-labeled peptides was acidified and fractionated using a high pH reversed-phase peptide fractionation kit (ThermoFisher) according to the manufacturer’s recommendation ...
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bioRxiv - Immunology 2023Quote: Phosphorylated peptides were enriched from 10uG total peptides using a High-Select Fe-NTA Phosphopeptide Enrichment Kit (Thermo Scientific A32992), according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... anhydrous peptide digests were reconstituted in 50mM TEAB pH 8.5 and quantified by fluorometric peptide assay (23290, Thermo Fisher Scientific). Peptides were labelled with 11-plex TMT according to the manufacturer’s instructions with minor modifications (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... The peptide concentration in the HEK293T sample was measured using a Pierce Quantitative Fluorescent Peptide Assay kit (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Fixed cell layers were washed gently with PBS once before addition of endogenous biotin blocking solutions (Endogenous Biotin Blocking kit, Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... cells were blocked for one hour with mouse-on-mouse blocking reagent (ReadyProbes™ Mouse-on-Mouse IgG Blocking Reagent, 1:30 dilution, ThermoFisher, R37621) between antibody staining procedures.