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Citations for Thermo Fisher :
851 - 900 of 10000+ citations for C Reactive Protein Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... followed by normal blocking solution (goat serum, Invitrogen) for 2 h at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... and PBS SuperBlock blocking buffer from Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... followed by blocking with 10% goat serum (Invitrogen) for 60 minutes at RT ...
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bioRxiv - Microbiology 2022Quote: ... in casein in PBS blocking buffer (Thermo Fisher) and 100 μL was added to the wells and allowed to incubate at room temperature for one hour ...
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bioRxiv - Immunology 2022Quote: ... and CellBlox blocking buffer (1:30 dilution, ThermoFisher), for 20 mins at 4°C in the dark ...
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bioRxiv - Neuroscience 2022Quote: Blocking buffer: 5% horse serum (Gibco, Life Technologies), 0.3% Triton X-100 (Roche) ...
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bioRxiv - Neuroscience 2022Quote: Blocking buffer: 5% horse serum (Gibco, Life Technologies), 0.3% Triton X-100 (Roche) ...
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bioRxiv - Developmental Biology 2022Quote: ... Blocking was performed using superblock (Thermo scientific, # 37580) for 2 hour at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... After blocking with 10% FBS (Thermo Fisher Scientific) in PBS for 1 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Avidin/Biotin Blocking Kit (Invitrogen, Carlsbad, CA, USA). After blocking with 10% normal goat serum (Vector Laboratories ...
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bioRxiv - Immunology 2024Quote: ... Samples were blocked with ELISA blocking diluent (Invitrogen) for 2 hr at 37°C then washed 3X ...
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bioRxiv - Immunology 2023Quote: ... antibodies diluted in BlockAid Blocking Solution (Thermo Fisher). Isotype specific anti−mouse ...
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bioRxiv - Cell Biology 2022Quote: ... Blocking was done using 5% goat serum (Invitrogen) for 2 hours at RT ...
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bioRxiv - Plant Biology 2023Quote: ... supplemented with 20% of blocking agent (RNAlater, Thermofisher scientific ...
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bioRxiv - Cell Biology 2023Quote: ... and blocked with SuperblockTM Blocking Buffer (Thermofisher #37581) with 0.1% TritonX-100 for 2 hours at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... and SuperBlockTM blocking buffer (Thermo Fisher Scientific, USA) for 30 minutes for blocking excess binding sites ...
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bioRxiv - Molecular Biology 2024Quote: ... using undiluted StartingBlockTM (TBS) Blocking buffer (Thermo Scientific). After washing ...
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bioRxiv - Cell Biology 2024Quote: ... followed by blocking in Superblock solution (Invitrogen, USA) and incubated with antibodies for 1 hour at RT in the humid chamber ...
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bioRxiv - Bioengineering 2024Quote: ... an endogenous biotin blocking kit (Invitrogen, cat#: R37628) was used according to manufacturer instructions prior to incubation with primary antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were centrifuged for 15 minutes at 13 000 g at 4°C and the supernatant was stored at -80°C before analysis for total protein concentrations using the Bradford protein assay (Thermo Fisher Scientific, Leicestershire, UK, 23200). Protein in each sample was quantified from a standard curve using BSA standards (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Lysates were centrifuged at 15,000 g for 10 min at 4 °C and the supernatant was quantified for total protein concentration using the Pierce BCA Protein Assay (Thermo Fisher Scientific, Cat. No. 23225). Thirty micrograms of protein were loaded onto sodium dodecyl sulfate−polyacrylamide gel for electrophoresis (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Lysates were centrifuged at 15,000 × g for 10 min at 4 °C and the supernatant was quantified for total protein concentration using the Pierce BCA Protein Assay (Thermo Fisher Scientific, Cat. No. 23225). Total protein 20 ug were separated by SDS-PAGE and further transferred onto 0.2 um PVDF membrane (Millipore ...
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bioRxiv - Systems Biology 2021Quote: ... total peptide concentration in each sample was determined using the Pierce™ Quantitative Fluorometric Peptide Assay (Thermo Fisher Scientific) to ensure that the same total peptide amount across samples 1-17 and 28-35 (excluding samples 18-27 ...
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bioRxiv - Plant Biology 2021Quote: ... The peptide concentration of the reconstituted solution was determined by using Pierce quantitative colorimetric peptide assay (Thermo Scientific, USA).
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bioRxiv - Biophysics 2022Quote: ... and the modified peptides were used for immunoaffinity purification according to manufacturer’s instructions (SulfoLink Immobilization Kit for Peptides, ThermoFisher). 72 µL of PAS beads was incubated overnight with 100 µg purified SMC1 antibody and 100 µg purified SMC3 antibody ...
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bioRxiv - Systems Biology 2023Quote: ... peptides were quantified by BCA assay and 150 μg of peptide per sample were labeled with TMTPro reagents (ThermoFisher) for 2 hrs at room temperature ...
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bioRxiv - Microbiology 2022Quote: The maleimide labeling of the HR2 peptides was performed by mixing 100 µM peptide and 2.5 mM fluorescein-5-maleimide (ThermoFisher) in degassed buffer (50 mM sodium phosphate ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were resuspended in running solvent and quantitated using a Pierce Colorimetric Peptide Quantitation Assay (Thermo Fisher Scientific, 23275).
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bioRxiv - Biochemistry 2024Quote: ... Peptides were resuspended in running solvent and quantitated using a Pierce Colorimetric Peptide Quantitation Assay (Thermo Fisher Scientific, 23275). Peptides were separated and analyzed as described above ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and peptides were purified using HiPPR detergent removal kit and Pierce peptide desalting spin columns (both Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants containing the obtained peptides were purified using Pierce™ Peptide Desalting Spin Columns (Thermo Scientific, Bartlesville, OK, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Phosphorylated peptides were enriched from 1mg of total peptides using High-Select Fe-NTA Phosphopeptide Enrichment Kit (Thermo Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... and peptide quantitation was performed using the Pierce™ Quantitative Peptide Assays & Standards (Thermo Fisher Scientific, Waltham, United States).
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bioRxiv - Physiology 2024Quote: ... peptides were reconstituted in 0.1% formic acid and used in the Pierce Quantitative Colormetric Peptide Quantification Kit (Thermo Scientific).
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bioRxiv - Physiology 2024Quote: ... Peptide samples were reconstituted in 0.07% triflouroacetic acid (TFA) and quantified using Colormetric peptide assay (23275, Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2024Quote: ... Stable isotope-labeled (heavy) peptides and synthetic unlabeled (light) peptides for targeted proteomics assay were purchased from Thermo Fisher Scientific (Rockford ...
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bioRxiv - Plant Biology 2022Quote: Coding sequences of mature proteins without signal peptide were amplified with primers listed in Table S1 and cloned into expression vector pETSUMO (Thermo Fisher Scientific, Massachusetts, USA). Correctness of the resulting constructs pETSUMO-ChElp2 and pETSUMO-Vd2LysM were confirmed by DNA sequencing and introduced into E ...
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bioRxiv - Immunology 2022Quote: ... and the QI9 peptide (QYIKWPWYI, residues 1208-1216 of the SARS-CoV-2 spike protein) maintained in RPMI 1640 medium (Thermo Fisher Scientific, Cat# 11875101) containing 10% FBS and 30 U/ml recombinant human IL-2 (Peprotec ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were probed with primary antibodies in SuperBlock T20 (TBS) Blocking Buffer and fluorophore-conjugated secondary antibodies in SuperBlock (TBS) Blocking Buffer (Thermo Scientific), with 3 washes in SuperBlock T20 (TBS ...
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bioRxiv - Cell Biology 2020Quote: ... Both NHS-reactive chemicals were resuspended immediately prior to the reaction in a new vial of anhydrous dimethylsulfoxide (DMSO, Invitrogen, D12345). The reaction was rocked for one hour at room temperature ...
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bioRxiv - Biochemistry 2019Quote: CMG2-GST (AlexaFluor 488 or biotin) and PA-E733C (AlexaFluor 546) were conjugated on a single reactive cysteine with maleimide-labels (Thermo Fisher) after GST was cleaved by using thrombin ...
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bioRxiv - Cancer Biology 2022Quote: ... cocultured mCherry+/CD133high cells and Wasabi+/SCCs were processed to single cells and labeled with fixable live/dead near-infrared fluorescent reactive dye (Invitrogen, L34975). The percentages of dead cells (live/dead dye+ ...
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bioRxiv - Immunology 2020Quote: ... Cells were then fluorescently barcoded (Krutzik and Nolan, 2006) using a combination of different concentrations of aminoacid reactive dyes (PacificBlue #10163, DyLight800 #46421, Thermo Scientific). Finally ...
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bioRxiv - Cell Biology 2019Quote: The level of the reactive oxygen species (ROS) in cells was determined with the DCF (2,7-dichlorofluorescein) assay (Life Technologies, Poland). For experiments ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed with FACS buffer consisting of PBS + 1% FCS and incubated for 30 minutes at room temperature with optimal dilutions of the amine-reactive Live/Dead Fixable Red viability cell stain (Life Technologies) and the antibodies CD4 PerCP-Cy5.5 ...
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bioRxiv - Microbiology 2022Quote: ... By measuring the absorbance of the supernatant at maximum wavelength for the Reactive Violet 5R (λmax = 558nm) and Methyl red (λmax = 420 nm) using Spectronic 20D+ (Thermo Scientific) the decolorization percentage was calculated using below equation,
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bioRxiv - Plant Biology 2022Quote: The detection and quantitation of reactive oxygen species (ROS) was performed by monitoring luminescence using a microplate luminometer (Luminoskan, Thermo Fisher). Leaf discs (0.5 cm diameter ...
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bioRxiv - Cell Biology 2023Quote: ... The carboxyl groups on the bead surfaces were functionalized with amine-reactive groups via N- ethyl-N’-(3-(dimethylamino)propyl)carbodiimide (EDC) and sulfo-N-hydroxysuccinimide (sulfo-NHS, Thermo Fisher) crosslinking for 20 minutes at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Reactive oxygen species (ROS) were labeled using the CellROX™ Green Reagent according to the manufacturer instructions (Molecular Probes, Eugene, USA). CellROX™ Green predominantly detects hydroxyl radicals and superoxide anions and only to low extent tert-butyl-hydroperoxide and do not detect hydrogen peroxide (www.thermofisher.com)[34,93].
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bioRxiv - Molecular Biology 2023Quote: ROS assay was performed using a Total Reactive Oxygen Species Assay Kit 520nM (Cat. Nr. 88-5930-74, Thermo Fisher Scientific) following the manufacturer’s instructions ...