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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for C Reactive Protein Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Cells were grown to early logarithmic phase at 37 °C in Protein Expression Medium (Gibco) or M9 minimal medium supplemented with 15NH4Cl (for expressing uniformly 15N labeled protein) ...
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bioRxiv - Genetics 2021Quote: The coding sequence for the pH insensitive Citrine fluorescent protein was inserted in the coding sequence of the BK2L3 protein (Zm00001d034049) immediately after the N-terminal secretory peptide domain and synthesized in the pDONOR221 vector (Life Technologies, USA) (Tian et al. ...
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bioRxiv - Neuroscience 2019Quote: Each tryptic peptide mixture obtained from 150 μg of protein extract was isotopically labeled with the corresponding TMT6plex reagent (Thermo Fisher Sci.) based on the standard procedure ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were sequenced by Edman Degradation using a Perkin Elmer 494 Procise Protein/Peptide Sequencer with an on-line 140C PTH Amino Acid Analyzer (Applied Biosystems, Inc).
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bioRxiv - Immunology 2024Quote: ... A polyclonal antibody against the ROTR-1 protein was raised using the peptide LDRSPPSDDGTQKV (ROTR-1 amino acids 288 to 301) in rabbit (Thermo Fisher Scientific). Anti-ROTR-1 sera was used at a dilution of 1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... The concentration of peptides was determined by measuring UV absorbance at 280 nm or by performing Pierce rapid gold BCA protein assay (Thermo Fisher Scientific) in case of peptides lacking aromatic amino acids ...
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bioRxiv - Microbiology 2023Quote: ... was added to cultures to label new peptide synthesis before harvesting (Click-iT™ Plus OPP Alexa Fluor™ 488 Protein Synthesis Assay Kit, Invitrogen)88 ...
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bioRxiv - Bioengineering 2023Quote: ... The concentration of peptide conjugates was determined by the standard Bicinchoninic acid (BCA) assay using Pierce(tm) BCA Protein Assay Kit (Thermo Fisher Scientific). To confirm successful synthesis of peptide conjugates ...
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bioRxiv - Microbiology 2023Quote: ... Peptide sequences (and hence protein identity) were determined by comparing the fragmentation pattern acquired by the software program Sequest (Thermo Fisher Scientific) (64 ...
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bioRxiv - Immunology 2022Quote: ... the RBD was inserted into pcDNA3.4 with a streptavidin binding peptide (SBP) tag at the C-terminus and were produced using the Expi293 Expression System (Thermo Fisher Scientific, Waltham, MA) according to manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: ... then incubated with the click chemistry reaction mixture containing an AlexaFluor 488 reactive azide (Thermo Fisher Scientific, cat #A10266) for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2019Quote: The production of reactive oxygen species (ROS) during amoeba predation was measured using dihydroethidium (DHE; Thermo Fisher, Dreieich, Germany) at a final concentration of 10 µM ...
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bioRxiv - Biophysics 2021Quote: Endogenous reactive oxygen species (ROS) were quantified with the fluorescent stain CellROX Orange (Thermofisher, excitation/emission 545/565 nm) to determine impact of nutrient limitation on ROS production ...
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bioRxiv - Microbiology 2019Quote: ... 900µL of the phage prep was combined with 90µL sodium bicarbonate (1M, pH = 9.0) and 10µL (1mg/mL) amine reactive Alexa-633 probe (ThermoFisher, Waltham MA) and incubated at room temperature for 1 hour ...
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bioRxiv - Biophysics 2020Quote: Recombinant GST-tagged PH-domain of PLCd detecting the membrane lipid PI(4,5)P2 was produced and conjugated to of amine-reactive Alexa Fluor 647 carboxylic acid succinimidylester (Invitrogen) as previously described49 ...
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bioRxiv - Cancer Biology 2021Quote: ... total intracellular reactive oxygen species (ROS) levels in Cys2-deprived cells were determined using CellROX® (Thermo Fisher Scientific). Approximately 105 cells were seeded in 12-well plates ...
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bioRxiv - Immunology 2022Quote: ... and soluble anti-CD28 (1µg/ml) for the indicated time before harvesting and staining with near-IR fluorescent reactive dye (Invitrogen).
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bioRxiv - Cancer Biology 2022Quote: ... Compensation beads (#01-2222-41) and ArC™ Amine Reactive Compensation Bead Kit (#10628) were purchased from Thermo Fisher. The cells were analyzed using a Cytek Aurora 5 Laser Spectral Flow Cytometer (Sony Biotechnology).
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bioRxiv - Microbiology 2020Quote: ... Purified vesicles were covalently labelled with an amine-reactive Alexa Fluor 488 5-SDP ester dye (Molecular Probes/ThermoScientific).
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bioRxiv - Microbiology 2021Quote: ... Reactive bands were visualised using HRP-conjugated secondary antibodies and SuperSignal West Pico or Dura chemiluminescent substrates (Thermo Scientific). Typically ...
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bioRxiv - Cell Biology 2020Quote: To assess mitochondrial membrane potential and reactive oxygen species (ROS) production multiple markers were used including MitoSOX™ (Invitrogen), Mitotracker® Red CMXRos (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... The presence of reactive oxygen species (ROS) was measured with the H2-DCF assay (Thermo Fisher Scientific, Waltham, MA). Lipid peroxidation was detected with the lipid peroxidation malondialdehyde (MDA ...
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bioRxiv - Immunology 2019Quote: ... washed with 1% BSA 0.2 mM EDTA in PBS and incubated with Fixable Live/Dead Aqua fluorescent reactive dye (L34966, Invitrogen), anti-mouse CD16/CD32 (Fc Block ...
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bioRxiv - Plant Biology 2019Quote: OefDef1.1 was labeled with DyLight 550 amine reactive dye following the protocol provided by the manufacturer (Thermo Fisher Scientific). Time-lapse confocal laser scanning microscopy was performed to monitor uptake and subcellular localization of the fluorescently labeled peptide as described previously (Islam et al. ...
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bioRxiv - Biochemistry 2020Quote: The Dip1 construct used for site specific labeling with the cysteine-reactive Alexa Fluor 568 C5 maleimide (Thermo Fisher) had the six endogenous cysteine residues in Dip1 mutated to alanine and a single cysteine added to the short N-terminal polypeptide sequence on the end of the coding sequence as previously described (Balzer et al. ...
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bioRxiv - Microbiology 2019Quote: ... Reactive bands were visualised using HRP-conjugated secondary antibodies and SuperSignal West Pico or Dura chemiluminescent substrates (Thermo Scientific). Typically 10–20 μg total protein was loaded per lane.
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bioRxiv - Immunology 2022Quote: ... twice then stained at 1:1000 with Zombie UV Fixable Live/Dead (blue-fluoresent reactive dye #L23105A; Thermofisher Scientific) for 15 minutes at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then washed twice in ice-cold PBS and stained with viability dye (violet fluorescent reactive dye, Cat:L34955, Invitrogen). FACS Buffer (1% FBS ...
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bioRxiv - Biophysics 2024Quote: ... and incubating the GHSR containing a single reactive cysteine at position 711.60 29 with Alexa Fluor 488 maleimide (ThermoFisher) at a 1:1.5 receptor-to-dye molar ratio ...
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bioRxiv - Cell Biology 2023Quote: ... then incubated with the click chemistry reaction mixture containing an AlexaFluor 488 reactive azide (Thermo Fisher Scientific, cat #A10266) for 30 minutes at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... Bovine fibrinogen is labeled with a fluorescent reactive dye (Alexa Fluor 647 NHS succinimidyl ester, Thermo Fisher Scientific, Waltham). After purification from the unreacted dye via dialysis ...
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bioRxiv - Genomics 2020Quote: ... SDS) for 10 minutes at 95°C and protein concentration was determined using the Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific 23227). Protein samples were separated by SDS-PAGE using 4-20% Mini-PROTEAN® TGX™ gels (BioRad ...
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bioRxiv - Genetics 2020Quote: ... This mixture was incubated overnight at 4°C with beads (Protein A Dynabeads, Invitrogen, for CTCF ChIP; Protein G Dynabeads, Invitrogen, for H3K9me3 ChIP) that had been pre-blocked with 0.5% BSA and mixed with polyclonal CTCF antibody (C15410210-50 ...
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bioRxiv - Genomics 2022Quote: ... for 30 minutes at 4°C and supernatant was retained and protein concentration quantified using the Pierce BCA protein assay kit (Thermo Fisher Scientific, 23227). A total of 25-35μg proteins were loaded onto 12.5% Tris-HCl gel for SDS-PAGE analysis ...
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bioRxiv - Neuroscience 2023Quote: ... Homogenized samples were centrifuged at 14,000 rpm for 15 min at 4°C and the protein concentration of the supernatant was determined by Pierce BCA Protein Assay (ThermoFisher Scientific, cat# 23227). Samples were diluted and supplemented with SDS sample buffer (50 mM Tris-HCl pH 6.8 ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were centrifuged for 10 min at 10,000 times gravity (ξ g) at 4 °C insoluble materials and total protein concentration was measured by the PierceTM BCA protein assay (Thermo Fisher Scientific). 15-20 µg of samples was prepared in Laemmli buffer and run in 8% SDS-PAGE before being transferred onto nitrocellulose membrane at 320 mA for 2.5 h ...
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bioRxiv - Cell Biology 2022Quote: ... The lysates were cleared by centrifugation at 18,000 x g and 4°C for 20 min and protein concentration was determined using the BCA Protein Assay Kit from Pierce™ (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... centrifuged at 16,000 x g for 15 minutes at 4°C and protein content determined by Pierce BCA protein assay kit (Thermo Fisher Scientific 23225). 25µg total lysate was removed as input and 0.5mg total protein was incubated with pre-washed magnetic M2 anti-FLAG (Sigma-Aldrich M8823-5ML ...
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bioRxiv - Developmental Biology 2021Quote: ... Then embryos were incubated in the blocking solution for at least 4 hours and incubated overnight at 4°C with secondary antibodies anti-chicken-AF488 (Invitrogen, A11039, used at 1/800), anti-rabbit-HRP (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: Pooled peptides were fractionated by a high-pH reversed-phase peptide fractionation kit (84868, Thermo Fisher, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... The digested peptides were fractionated using a high-pH reversed phase (RP) peptide fractionation kit (Thermo Scientific). Fractions collected from the high-pH RP fractionation were further separated using on-line second-dimension low-pH reversed-phase chromatography via an Easy-nLC 1200 nanoflow-UHPLC system (Thermo Scientific ...
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bioRxiv - Biochemistry 2020Quote: Pooled peptides were fractionated using a high-pH reversed-phase peptide fractionation kit (84868, Thermo Fisher, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Peptide concentration was quantified with a plate reader using the Pierce Quantitative Colorimetric Peptide Assay (Thermo Scientific). Finally ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 25 fmol/µl standard peptide mixture (Pierce™ Peptide Retention Time Calibration Mixture, ThermoFisher, 88321) and diluted to a final volume of 23 µl with water ...
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bioRxiv - Pathology 2024Quote: ... The concentration of the peptides was determined using Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific, Cat#23275). Tandem mass tag (TMT)-based labeling of peptides was performed using the TMT10plex™ Isobaric Label Reagent Set (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... and the supernatant was recovered for peptide desalting using Pierce™ Peptide Desalting Spin Column (ThermoFisher: 89851) as described by the manufacturer ...
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bioRxiv - Microbiology 2024Quote: ... 0.1% TFA and peptide content was estimated with a Pierce™ Quantitative Colorimetric Peptide Assay (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The peptide concentration was measured using the Pierce™ Quantitative Colorimetric Peptide Assay (Thermo Scientific cat. # 23275). The digested peptides were subjected to simple C18 clean up using a C18 disk plug (3M Empore C18 ...
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bioRxiv - Neuroscience 2023Quote: ... desalted peptides was separated into 9 fractions with the high pH reversed-phase peptide fractionation kit (ThermoFisher). Fractions with increasing percentages of acetonitrile (ACN ...
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bioRxiv - Microbiology 2023Quote: ... the concentration of peptides was measured using Pierce Quantitative fluorometric peptide assay (ThermoFisher Scientific, Rockford, IL, USA).The resulting peptides were sequenced on a Q-Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (ThermoFisher Scientific ...