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Citations for Thermo Fisher :
1001 - 1050 of 10000+ citations for C Reactive Protein Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: Cellular reactive oxygen species (ROS) and mitochondrial ROS (mROS) were detected using fluorescence probe CM-H2DCFDA (Invitrogen; Thermo Fisher Scientific, Inc., USA) and MitoSOX Red (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: Cellular reactive oxygen species (ROS) and mitochondrial ROS (mROS) were detected using fluorescence probe CM-H2DCFDA (Invitrogen; Thermo Fisher Scientific, Inc., USA) and MitoSOX Red (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... The pHrodo-TDM1 was obtained by conjugating the free lysine residues of TDM1 with the amine-reactive pH-sensitive pHrodo iFL Red STP ester dye (ThermoFisher Scientific, P36014) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2021Quote: ... EdU and the reactive Alexa Fluor™ 488 dye used according the manufacturer instructions to Click-iT™ EdU Imaging Kit (cat.no. C10086; Invitrogen, USA). Five regenerates (cross section of the body ...
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Patient-associated mutations in Drosophila Alk perturb neuronal differentiation and promote survivalbioRxiv - Cancer Biology 2022Quote: ... The remaining cells were respectively labelled with aqua-fluorescent reactive dye (dying cells) and calcein violet AM (living cells) using the LIVE/DEAD Violet Viability/Vitality Kit (Molecular Probes, #L34958) under manufacturer’s guidelines ...
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bioRxiv - Immunology 2022Quote: ... OEP light cages were applied to export individual cross-reactive mAb secreting B cells directly into Dynabeads mRNA DIRECT lysis buffer (Life Technologies, 61011) in 96-well plates ...
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bioRxiv - Developmental Biology 2019Quote: The compound 5-(and-6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA, Image-iT LIVE Green Reactive Oxygen Species Detection Kit, Molecular Probes; Invitrogen, I36007) was used to visualise the in vivo production of ROS ...
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bioRxiv - Genetics 2023Quote: ... We gated for cells and live cells based on the Near-IR fluorescent reactive dye LIVE/DEADTM (LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit, Invitrogen, L10119). Spermatogonia were identified and gated within the diploid (2C ...
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bioRxiv - Cell Biology 2024Quote: The amount of reactive oxygen species (ROS) produced within the cell was measured using CellROX™ Deep Red Reagent for oxidative stress detection (Invitrogen, USA). The cells were cultured in glass slides (ibidi ...
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bioRxiv - Biophysics 2021Quote: ... pH 8.0) at 4°C overnight in 10,000 MWCO Thermo protein Slide A Lyzer (Fisher Scientific cat#PI87730). Following dialysis ...
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bioRxiv - Cell Biology 2020Quote: ... 1000 μg of total protein was incubated overnight at 4 °C with 2 μg α-GFP (Life Technologies/ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... and the remainder was incubated overnight at 4°C under rotation with 100ul of protein G dynabeads (Invitrogen) pre-loaded for 4hrs at RT with 5ug of ChIP antibodies diluted in shearing buffer containing 0.15% SDS (see key reagents) ...
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bioRxiv - Cell Biology 2021Quote: ... diluted cell supernatant was preabsorbed for one hour at 4°C with protein A/G magnetic beads (Invitrogen). The supernatant fraction was incubated overnight at 4°C with an appropriate antibody and protein A/G magnetic beads were blocked with 4% BSA ...
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bioRxiv - Plant Biology 2021Quote: ... and incubated for 1 h at 4°C with 40 μL of Protein AG UltraLink Resin (Thermo Fisher). The beads were washed 2 × 5 min in ChIP Wash Buffer 1 (0.1% SDS ...
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bioRxiv - Plant Biology 2021Quote: ... for 1 h with gentle rotation at 4°C and then protein-G coated magnetic beads (Thermo Fisher) were added and incubated for an additional 30 min ...
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bioRxiv - Genomics 2020Quote: ... Samples were incubated for 1h at 4°C with Dynabeads Protein G for Immunoprecipitation (#10003D, Invitrogen, ThermoFisher Scientific). Final DNA was purified with MinElute (#28004 ...
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bioRxiv - Genomics 2020Quote: ... Samples were incubated for 1h at 4°C with Dynabeads Protein G for Immunoprecipitation (#10003D, Invitrogen, ThermoFisher Scientific). Final DNA was purified with MinElute (#28004 ...
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bioRxiv - Plant Biology 2021Quote: ... Total protein extracts were then mixed with Pierce™ anti-c-Myc magnetic beads (Thermo Fisher Scientific, USA) and incubated at 4°C with gentle agitation for 1 h ...
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bioRxiv - Developmental Biology 2019Quote: ... and the rest was immunoprecipitated at 4°C for 2 h using Dynabeads Protein A (Life Technologies, # 10008D)-conjugated anti-m6A antibody (Synaptic systems ...
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bioRxiv - Plant Biology 2020Quote: ... and incubated for 1 h at 4°C with 40 µL of Protein AG UltraLink Resin (Thermo Scientific). The beads were washed 2 × 5 min in ChIP Wash Buffer 1 (0.1% SDS ...
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bioRxiv - Bioengineering 2021Quote: ... Purified proteins were dialyzed against phosphate-buffered saline (PBS) using dialysis cassettes at 4°C (Thermo Fisher Scientific). Spike was dialyzed with a 20 kDa molecular weight cutoff (MWCO ...
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α-catenin links integrin adhesions to F-actin to regulate ECM mechanosensing and rigidity-dependencebioRxiv - Cell Biology 2021Quote: ... #101500]) overnight at 4°C and subsequently rotated with Pierce™ Protein G Magnetic Beads (Thermo Fisher, #88848) for 1h at 4 °C ...
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bioRxiv - Genetics 2020Quote: ... SDQ2357) incubated over-night rotating at 4°C together with 80µl magnetic protein A/G beads (Thermo Scientific). Samples were then washed twice in 150mM NaCl buffer ...
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bioRxiv - Microbiology 2020Quote: ... SARS2-S or MERS-S - C-terminally fused to the green fluorescence protein (GFP) - using Lipofectamine 2000 (Invitrogen). Two days post transfection ...
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bioRxiv - Developmental Biology 2022Quote: ... Beads-protein complexes were then heated (50°C, 10min) in NuPAGE™ LDS Sample Buffer (4X) (Invitrogen, NP0008). Samples were magnetized prior supernatants collection and incubated (70°C ...
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bioRxiv - Microbiology 2022Quote: The recombinant proteins named rS1Beta was purified using a CaptureSelect™ C-tagXL Affinity Matrix prepacked column (ThermoFisher) and followed the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2022Quote: ... 4 °C) and the concentration determined using a BCA assay (Pierce™ BCA Protein Assay Kit, Thermo Scientific). After running an SDS PAGE (12%) ...
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bioRxiv - Molecular Biology 2022Quote: Protein concentrations were measured using the A205 preprogrammed direct absorbance application of Nanodrop One C (Thermo Fisher Scientific). Measurements were performed following acetic acid incubation and after the mechanical crushing to exclude the possibility of protein loss in washes.
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bioRxiv - Plant Biology 2024Quote: ... The EIN2-C native antibody was prebound with the equilibrated protein-G coated magnetic beads (Dynabeads, Thermo Fisher) with 1% BSA for 3 h with gentle rotation at 4 °C and incubated at 4 °C after cleared cell fractionation addition with gentle rocking overnight for Co-IP ...
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bioRxiv - Molecular Biology 2024Quote: ... FLAG M2 or HA-tag antibody at 4 °C overnight before precipitated with magnetic Protein G beads (Thermofisher) for 2 hours at 4 °C followed by 3 washes with RIPA buffer.
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bioRxiv - Molecular Biology 2024Quote: ... Immune complexes were pulled down for 45 min at 4°C with 25 µL protein G dynabeads (Invitrogen), captured on a magnet and washed 4 times in 800 µL IP buffer ...
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bioRxiv - Cell Biology 2024Quote: LZTR1WT and LZTR1L580P were expressed as C-terminal His-tagged proteins in Expi-293F cells (Thermo Fisher Scientific). pcDNA3.1-LZTR1-Myc-6xHis plasmid was a gift from Jens Kroll (Heidelberg University and German Cancer Research Center (DKFZ-ZMBH Alliance)).56 The LZTR1L580Pvariant was introduced into the plasmid by site-directed mutagenesis as previously described.11 Cells were transfected using ExpiFectamine 293 Reagent (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... were pre-bound overnight at 4°C to 60 μl of Dynabeads protein G (10003D, Thermo Fisher Scientific) diluted in 250 μl of blocking buffer (PBS ...
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bioRxiv - Microbiology 2024Quote: ... 4 °C) and protein concentration in the supernatant was determined by a commercially available BCA assay (ThermoFisher Scientific). The same protein amount was used for each measurement.
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bioRxiv - Cell Biology 2024Quote: ... protein samples were denatured at 70°C for 10 min in LDS Sample Buffer (Thermo Fisher Scientific, NP0007) with or without 100 mM Dithiothreitol (+DTT and –DTT ...
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bioRxiv - Neuroscience 2022Quote: ... Protein samples were incubated at 100°C for 5 min with NuPAGE Sample loading dye (Thermo Fisher, NP0007) and dithiothreitol as reducing agent ...
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bioRxiv - Genomics 2023Quote: ... The chromatin was incubated overnight at 4°C with 50 μl Protein A magnetic beads (Dynabeads, Thermo Fisher) pre-bound with 2.5 μl α-CENH3 (gift of Prof ...
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bioRxiv - Cell Biology 2023Quote: ... 1:2 diluted whole cell extract in buffer C was added to Dynabeads Protein G (Thermo Fisher Scientific) pre-incubated with HEXIM1 antibody ...
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bioRxiv - Cell Biology 2023Quote: ... 4°C) and the protein concentration in cellular lysates was determined by absorbance on a nanodrop instrument (ThermoFisher). 1 mg of lysate in 1 mL of lysis buffer was incubated overnight at 4°C with 20 μL of anti-FLAG M2 magnetic bead slurry (MilliporeSigma ...
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bioRxiv - Cancer Biology 2024Quote: ... at 100°C and 15 μg of protein were separated with 4–12% Bis-Tris gels (Invitrogen, US) and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Immunology 2024Quote: ... 5μg of protein were boiled for 2min at 85°C in NovexTM Tricine SDS Sample Buffer 2X (Invitrogen), separated on NovexTM 10%-20% Tricine Protein Gels (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: Phospho-peptides were fractionated using alkaline reversed phase chromatography (Pierce™ High pH Reversed Phase Peptide Fractionation Kit, Thermo Scientific #84868) into 12 fractions using increasing concentrations of acetonitrile from 10%-80% in basic binding buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... All ten vials of samples were combined and approximately 100 μg peptide digests from this mix were fractionated using Pierce High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific) according to instructions from manufacturer ...
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bioRxiv - Biochemistry 2020Quote: ... samples were briefly centrifuged at 13,000 g and peptide-containing supernatant was used for further analysis Peptides were fractionated into eight fractions using High-pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher). Peptide fractions were dried in SpeedVac (Eppendorf ...
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bioRxiv - Bioengineering 2021Quote: ... then incubated with 50 µL of 1 µM biotinylated peptide in peptide binding buffer (1% sheared salmon sperm DNA (Invitrogen, AM9680), 10 mM HEPES ...
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bioRxiv - Cancer Biology 2021Quote: ... and remaining ~1.1 mg of multiplexed peptides were used for phospho-peptide enrichment by High-Select Fe-NTA Phosphopeptide enrichment kit (Thermo Fisher) after manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... TMT-labeled peptides were pre-fractionated with the basic pH reversed-phase C18 peptide fractionation kit following manufacturer’s manual (Thermo Fisher, 84868). Typically ...
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bioRxiv - Biochemistry 2019Quote: ... peptide concentration and primary amine reactivity were quantified using the Pierce quantitative fluorometric peptide assay (Thermo Fisher Scientific; cat. no. 23290) and Pierce quantitative colorimetric peptide assay (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... were raised in rabbits by immunization with keyhole limpet hemocyanin-conjugated peptides and affinity-purified by columns conjugated with the peptides (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: The phosphopeptide enrichment step flow through and washes were dried down and approximately 120 µg peptides were fractionated using the TMT fractionation protocol of Pierce high pH basic reversed-phase peptide fractionation kit (Thermo Fisher Scientific™ Cat no 84858 ...