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Citations for Thermo Fisher :
751 - 800 of 10000+ citations for C Reactive Protein Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and the supernatant was recovered for peptide desalting using Pierce™ Peptide Desalting Spin Column (ThermoFisher: 89851) as described by the manufacturer ...
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bioRxiv - Physiology 2024Quote: ... peptides were reconstituted in 0.07% trifluoroacetic acid and quantified by Fluorometric Peptide Assay (Thermo Fisher Scientific, 23290).
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bioRxiv - Molecular Biology 2024Quote: The TMT labeled peptides were fractionated using high pH reversed-phase peptide fractionation kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... and the peptides were separated using the High pH Reversed-Phase Peptide Separation Kit (Thermo Fisher Scientific) according to the instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... were used following biotin blocking of tissues samples using endogenous Biotin-blocking kit (Life Technologies Corporation/Thermo fisher, E21390). For Aza-peptide preparation ...
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bioRxiv - Molecular Biology 2021Quote: ... were used following biotin blocking of tissues samples using endogenous Biotin-blocking kit (Life Technologies Corporation/Thermo fisher, E21390). For Aza-peptide preparation ...
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bioRxiv - Cell Biology 2020Quote: ... and 1X PBS) before blocking for one hour with blocking buffer (Bovine Serum Albumin (Fisher Scientific, Fair Lawn, NJ), Normal Donkey Serum (Jackson ImmunoResearch Inc. ...
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bioRxiv - Physiology 2022Quote: ... Non-specific antigens were blocked for 60 min using a blocking buffer (Sea Block Blocking Buffer; ThermoFisher Scientific, USA). Then ...
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bioRxiv - Biochemistry 2020Quote: ... and with or without CLASPIN pS945 peptide (MEELLNLC(pS)GKFTSQ) (Peptide Protein Research Ltd, Bishops Waltham, UK) at different concentrations in a Thermoscientific 96 well UV microplate (Fisher scientific, Loughborough, UK). The plates were incubated at 25 °C and absorbance at 340 nm was measured in a POLARstar Omega multimode microplate reader (BMG Labtech GmbH ...
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bioRxiv - Microbiology 2021Quote: LC-MS/SM analysis of trypsin-digested proteins (peptides) was performed on an Orbitrap Q Exactive Plus mass spectrometer (Thermo Fisher Scientific, Bremen) coupled to an EASY-nLC 1200 (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... Peptide sequences were determined by matching protein databases with the acquired fragmentation pattern by the Sequest software program (Thermo Fisher Scientific, Waltham, MA) 57 ...
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bioRxiv - Cell Biology 2023Quote: ... Concentrations of proteins and peptides were calculated from the UV absorption at 280 nm using a Nanodrop spectrophotometer (Thermo Fisher Scientific, DE; USA).
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bioRxiv - Neuroscience 2020Quote: ... Lysate was centrifuged at 14,000g for 5min at 4°C and protein concentration was measured using Pierce BCA protein assay kit (Thermo Fisher Scientific, MA, USA PI23227) following manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: ... The total protein of the kidneys was extracted by centrifugation at 14,000g for 15 minutes at 4°C and protein concentration was determined using micro bicinchoninic acid (BCA) protein assay kit (Thermo Scientific, Rockford, Il, USA) according to manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2023Quote: ... The total protein concentration was determined by BCA assay and 500 μg of total proteins was incubated for 1 hour (4°C, rotary agitation) with 25 μl of Pierce™ Protein A Agarose beads (ThermoFisher, cat. no. 20333), centrifuged at 2500 g for 3 minutes at 4°C and the supernatant recovered as pre-cleared lysate ...
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bioRxiv - Biochemistry 2022Quote: ... were centrifuged at 16,000 g for 30 minutes at 4 °C and the supernatant was collected for protein estimation using Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific; Cat# 23227). Equal amounts of liver protein (100 μg ...
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bioRxiv - Cancer Biology 2023Quote: ... for 2hours at 4°C and this was followed by precipitation of the protein-antibody complex with dynabeads protein G (Invitrogen, #300D, Carlsbad, CA, USA) for 2h in 4°C on rotator ...
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bioRxiv - Plant Biology 2023Quote: ... The nuclear and cytosolic fractions were boiled in 1X Laemmli buffer (Laemmli, 1970) for 10mins at 100°C and protein was quantified using BCA compatible protein kit (Thermo Fisher Scientific, Whatham, USA). Fifty micrograms of protein were loaded onto a 4-20% gradient SDS-PAGE denaturing protein gel as described above ...
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bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFUSE-vector (Invitrogen, Thermo Fischer Scientific, MA, USA). The human ACE2 was expressed by essentially the same protocol used for the COVID-19 RBD ...
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bioRxiv - Neuroscience 2023Quote: Slices were stained for green fluorescent protein (GFP) with a rabbit monoclonal anti-GFP antibody (G10362, Molecular Probes, 1:1000 dilution in blocking buffer) and for tyrosine hydroxylase (TH ...
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bioRxiv - Molecular Biology 2021Quote: The peptide concentration was determined by using the Pierce Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific, Rockford, IL) and 180 µg peptides from each sample were loaded onto MacroSpin column (NestGroup ...
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bioRxiv - Microbiology 2021Quote: ... Labeled peptides of all samples were mixed equally and desalted by peptide desalting spin columns (Thermo Fisher, 89,852). A C18 column (Waters BEH C18 ...
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bioRxiv - Plant Biology 2022Quote: LC-MS/MS analysis of peptides and phosphopeptides – Peptides mixtures were analyzed by nanoflow Easy-nLC (Thermo Scientific) and Orbitrap hybrid mass spectrometer (Q-exactive HF ...
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bioRxiv - Systems Biology 2022Quote: ... Peptide concentrations were determined using a fluorimetric peptide assay kit following the manufacturer’s instructions (Thermo Scientific, Cat#23290).
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bioRxiv - Plant Biology 2019Quote: ... followed by peptide fractionation using the Pierce High pH Reverse-Phase Peptide Fractionation Kit (Thermo Scientific, Rockford, IL). Eight fractions of peptides from each sample were subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS ...
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bioRxiv - Cell Biology 2021Quote: ... Phospho-peptide enrichment was then performed using the High-SelectTM TiO2 Phospho-peptide Enrichment kit (Thermo Fisher Scientific). Each replicate was divided between two TiO2 columns and prepared according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Peptides were then desalted by reverse-phase chromatography using Pierce peptide desalting spin columns (Thermo Fisher part #89852). Final resuspension was done with Solvent A (0.1% formic acid in Fisher Optima LC/MS grade water ...
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bioRxiv - Plant Biology 2022Quote: ... Peptides were subsequently fractionated using high pH reversed-phase peptide fractionation kit (Thermo Fisher Scientific, San Jose, USA) and pooled into 8 fractions [43] ...
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bioRxiv - Microbiology 2023Quote: ... of 78 of 15-mer peptides covering DBV GnH formulated at 0.625μg/ml for each peptide in RPMI medium (Gibco) in the 96-well plate ...
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bioRxiv - Cell Biology 2024Quote: ... soluble peptide suspension was transferred into fresh Eppendorf tubes and peptide concentration was determined by microBCA (Thermo Scientific). Equal amounts of peptides for each sample (150-200 µg ...
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bioRxiv - Genetics 2024Quote: ... The concentration of tryptic peptides was measured using the Pierce Quantitative Peptide Assays kit (ThermoFisher Scientific cat# 23275), and 25 µg of peptides were separated on a home-made reverse-phase C18 column in a pipet tip ...
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bioRxiv - Biophysics 2023Quote: ... The concentration of peptide was determined using Pierce quantitative fluorometric peptide assay (Cat# 23290, Thermo Scientific, Rockford, IL). 11 – 18 μL of the peptides were analyzed through nano-ESI-LC/MS/MS using the Orbitrap Exploris mass spectrometer coupled to a Dionex nano-LC system (Thermo Scientific ...
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bioRxiv - Systems Biology 2023Quote: Phospho-peptide enrichment was carried out using the High-Select TiO2 Phospho-peptide Enrichment kit (Thermo Fisher, A32993), as per manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... The peptide concentration of this pool was determined using a fluorimetric peptide assay kit (Thermo Scientific, Cat#23290). Peptide concentrations per sample were estimated by multiplying the optical density recorded at harvest with the ratio between pool peptide concentration and the median at-harvest optical density.
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bioRxiv - Cancer Biology 2023Quote: The peptide amount in each sample was estimated using a quantitative colorimetric peptide assay kit (Thermo Fisher Scientific). Within each batch ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Peptides were quantified using a Pierce Colorimetric Peptide Quantification Assay Kit following the manufacturer’s instructions (Thermo Scientific, 23275). 50 μg of each sample was separated for multiplex proteomic analysis ...
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bioRxiv - Molecular Biology 2024Quote: ... Peptides reconstituted with 5% acetonitrile/0.1% formic acid were quantified using Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific) and 10 µg of peptides for each sample were labeled with 50 µg of TMTpro16-plex reagents (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... The peptide mix was then fractionated using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific). The 8 obtained fractions were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive HF ...
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bioRxiv - Genetics 2021Quote: ... Epitope-tagged bait proteins were detected with mouse anti-c-myc monoclonal antibody (Invitrogen, MA1-980) and IR-680 conjugated donkey anti-mouse secondary antibody (LI-COR ...
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bioRxiv - Immunology 2021Quote: ... and protein was incubated overnight at 4°C with a Pierce endotoxin removal column (88274, ThermoFisher). Protein was eluted by spin filtration ...
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bioRxiv - Cell Biology 2019Quote: ... The protein lysates were incubated over night at 4 °C with streptavidin agarose beads (Thermo Scientific) to precipitate LecB-biotin-protein complexes ...
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bioRxiv - Cancer Biology 2021Quote: ... Chromatin immunocomplexes were precipitated for 1 hour at 4°C with blocked Dynabeads Protein G (Invitrogen), subjected to multiple wash steps and the chromatin eluted in elution buffer (50 mM Tris-HCl [pH 8.0] ...
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bioRxiv - Immunology 2022Quote: ... for protein expression were maintained at 37°C and 8% CO2 in Expi293 Expression medium (GIBCO), transfected using Expi293 Expression System Kit (GIBCO ...
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bioRxiv - Microbiology 2022Quote: ... The protein with a C-terminal Strep-tag was expressed in Expi293 cells (Thermo Fisher Scientific) and subsequently purified using Strep-Tactin XT Superflow high capacity resin (IBA Lifesciences ...
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bioRxiv - Cancer Biology 2019Quote: ... followed by 3–4-hour incubation at 4°C with protein A/G agarose (20421, Invitrogen). The beads were then collected for western blot detection ...
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bioRxiv - Molecular Biology 2020Quote: ... 4°C and supernatant protein concentration was quantified with Enhanced BCA protocol (Thermo Fisher Scientific, Pierce). Equivalent amounts of proteins were separated on an SDS-page (approximately 30 µg ...
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bioRxiv - Plant Biology 2019Quote: Microsomal proteins were predigested for three hours with endoproteinase Lys-C (0.5 µg/µl; Thermo Scientific) at 37 °C in 6 M urea 2 M thiourea ...
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bioRxiv - Physiology 2021Quote: ... The reactions were then incubated for 1h at 4°C with protein A/G dynabeads (Invitrogen) pre-blocked in PBS/0.5% BSA/0.5% Tween ...
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bioRxiv - Microbiology 2020Quote: ... Bound protein was detected via the C-terminal 6XHis Tag using 6XHis Antibody MA1-135 (Invitrogen) followed by three washes in TBS-T and incubation with donkey anti-mouse horseradish peroxidase (HRP)-coupled secondary antibody (Dianova) ...
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bioRxiv - Biochemistry 2021Quote: ... followed by protein precipitation with 300 µL of cold (−20°C) acetonitrile (Thermo Fisher, Waltham, MA). The sample was vortexed again for 30 s and chilled for 30 min at −20°C ...