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Citations for Thermo Fisher :
9351 - 9400 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The cells were washed 3 times and then incubated with the goat anti-rabbit secondary antibodies Alexa Fluor® 488 (1:1,000, Thermo Fisher Scientific, USA) for ESCRT-III ...
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bioRxiv - Cell Biology 2022Quote: ... KIF5B (5’-CUACAUGAACUUACGGUU-3’) and non-targeting control siRNA (Silencer Select Negative Control No.1 siRNA Cat #4390843) were purchased from Ambion (Thermo Fisher Scientific). Cells were seeded in six-well plates at 30% confluence and transfected with 12.5-25 nM final siRNA concentration using Lipofectamine RNAiMax (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... the whole cell layer was digested for 3 hours with digestion solution containing 1 mg/mL of collagenase IV (Gibco, cat. no. 17104019) in TrypLE (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were rinsed (3 times) with 2 ml incubation buffer and resuspended with anti-mouse Alexa Fluor 647 secondary antibody (1:2000, Invitrogen, Carlsbad, CA, USA) for 60 minutes at RT ...
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bioRxiv - Microbiology 2022Quote: ... Tissues were incubated with primary antibodies (mouse anti-HuC/D IgG clone 16A11 at 1:200 [ThermoFisher], rabbit anti-tubulin β-3 (TuJ1) polyclonal IgG at 1:500 [Biolegend] ...
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bioRxiv - Neuroscience 2022Quote: ... HLA-DR Antibody (Monoclonal Rabbit Anti-Human, Citrate Buffer HIER, dilution 1:50 Clone: LN-3, Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA), TMEM119 (Rabbit Anti-Human ...
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bioRxiv - Cancer Biology 2024Quote: ... Enzymatic treatments were done in staining buffer supplemented with 3 mM magnesium chloride with 1:200 dilutions of RNase T1 (Thermo Fisher Scientific, EN0541), ShortCut RNase III (New England Biolabs ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were then rinsed 3 times in PBS and incubated with goat anti-rabbit Alexa Fluor 488 (1:200 dilution, Invitrogen, Carlsbad, CA, USA), goat anti-mouse Alexa Fluor 568 (1:200 dilution ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were then washed 3 times with PTw before incubating with the secondary antibody (goat anti-rabbit alexa488, 1:500, Thermo Fisher A-11008) and DNA stain (Hoechst34580 1:1000 Sigma Aldrich 63493) ...
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bioRxiv - Developmental Biology 2024Quote: ... HaCaT cell transfection with a 75 pM siRNA mix (#2316-1, #2316-2, and #2316-3) was conducted using Lipofectamine RNAiMAX transfection reagent (Invitrogen, Carlsbad, CA, USA), following the manufacturer’s guidelines.
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bioRxiv - Microbiology 2024Quote: ... and amplified product was also verified by adding 3 µl of SYBR Green I dye (1:9 dilution, Life technologies Corporation, Eugene, OR) in each sample tube ...
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bioRxiv - Biochemistry 2022Quote: ... 10 μL of each sample was injected on an Hypercarb column (100 × 1 mm, particle size 3 μm, Thermo-Fisher Scientific, Courtaboeuf, France) heated at 80 °C with a flow rate settled at 0.165 mL.min-1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Mesenteries were washed 3 times in 1× PBS and mounted on a slide using the ProLong Diamond Antifade Mountant (Thermo Fisher Scientific, P36961). Images were taken with a Nikon C2 confocal ...
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bioRxiv - Neuroscience 2023Quote: Fly brains expressing Atg8-mcherry were dissected in Schneinder’s media and stained for 3 minutes with lysotracker (LysoTracker Red DND-99; Thermo Fisher Scientific; 1:2000), rinsed in Schneinder’s media and then directly visualised with a Zeiss Airyscan 880 ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 7.4] for 3 hours at room temperature (RT) followed by incubating with a rabbit anti-EGFP antibody (ThermoFisher G10362, 1:1000 dilution) at 4 °C overnight ...
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bioRxiv - Microbiology 2023Quote: ... and 0.6 mL of ice cold isopropanol was added along with 1/10th volume sodium acetate solution (Invitrogen, 3 M, pH 5.2; R1181) and 1 μL GlycoBlue coprecipitant (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed with cold 1× PBS-T for 3 times each for 10 min then incubated for 2 hours at RT with secondary antibodies (AF546-Ms: Invitrogen A11003, 1:500) diluted in 1× PBS-T ...
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bioRxiv - Neuroscience 2022Quote: ... retinas were incubated for 3 hours at room temperature with Alexa Fluor-conjugated secondary antibodies (1:1,000; Thermo Fisher Scientific or Jackson ImmunoResearch). Whole retinas were flattened with photoreceptor side down onto nitrocellulose filters ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were washed 3-times with 1× PBS and stained with Alexa Fluor 568 goat anti-mouse IgG1 secondary antibody (Life Technologies, catalog # A21124) at room temperature for one hour ...
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bioRxiv - Cell Biology 2024Quote: ... The Triton-X permeabilization solution was removed and cells were washed 3×2 minutes with DPBS at RT before a blocking solution (1% BSA (Fisher Scientific, BP1600-100) was added to cells for one hour at RT with rocking ...
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bioRxiv - Neuroscience 2024Quote: ... Two-phase extraction was performed by adding 400 µl of methyl-tert-butyl ether (MTBE): methanol (3:1, v/v; all solvents of High-performance liquid chromatography (HPLC)-grade (Thermo Fisher Scientific, Germany)) ...
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bioRxiv - Neuroscience 2024Quote: ... in 1X PBS-0.3% Triton X-100 (0.3% PBST) before 2-3 day incubation with primary antibodies at 4C for PCP4 (1:250, Invitrogen Cat# PA5-52209, RRID:AB_2645298) or NECAB2 (1:250 ...
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bioRxiv - Microbiology 2021Quote: Keratinocytes were seeded at a density of 5×105 cells/well in a 6-well tissue culture plate (Nunc; Thermo Fisher Scientific, Singapore) and grown for 3 days at 37°C in a 5% CO2 humidified atmosphere ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then rinsed in PBS 6 times for 5 minutes each followed by incubation with goat anti-rabbit IgG Alexa Fluor 594 (#A32740, ThermoFisher Scientific, Waltham, MA) and goat anti-mouse IgG Alexa Fluor 488 (#A11008 ...
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bioRxiv - Microbiology 2021Quote: Keratinocytes were seeded at a density of 5×105 cells/well in a 6-well tissue culture plate (Nunc; Thermo Fisher Scientific, Singapore) and grown for 3 days at 37°C in a 5% CO2 humidified atmosphere ...
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bioRxiv - Developmental Biology 2022Quote: ... 95 °C for 5 seconds and 60 °C for 20 seconds in a QuantStudio 6 Flex Real-Time PCR machine (Thermo Fisher Scientific, USA). The detectors used were FAM-BHQ1 and HEX- BHQ1 ...
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bioRxiv - Immunology 2024Quote: ... followed by 40 cycles of denaturation at 95 °C for 5 sec and extension at 58 °C for 34 sec in the QuantStudio 6 system (Applied Biosystems Co., USA). At the end of PCR ...
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bioRxiv - Bioengineering 2020Quote: ... the coverslips were placed upside down on a glass slide with slow fade mounting medium containing 4’,6-diamidino-2- phenylindole (DAPI, Life Technologies, Carlsbad, CA, USA) and kept at 4 °C until observation ...
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FGF10 triggers de novo alveologenesis in a BPD model: impact on the resident mesenchymal niche cellsbioRxiv - Cell Biology 2022Quote: ... at RT and washed three times with TBST before being mounted with Prolong Diamond Anti-fade Mountant with DAPI (Invitrogen 4’,6-diamidino2-phenylindole). Photomicrographs of immunofluorescence staining were taken using a Leica DMRA fluorescence microscope with a Leica DFC360 FX camera (Leica ...
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bioRxiv - Microbiology 2021Quote: ... Nuclei were counterstained with Hoechst 33342 DNA dye NucBlue® Live ReadyProbes® reagent for live cell imaging or 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI) and SYTO® 60 fluorescent nucleic acid stain for fixed samples (Invitrogen, Molecular Probes, Germany).
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bioRxiv - Biochemistry 2020Quote: ... Coverslips were mounted and cells were counterstained on the glass slides using ProLong Gold with DAPI (4’, 6-diamidino-2-phenylindole) (Molecular Probes, Eugene, OR, US). Images were collected using an Axio Plan2/AxioCam microscope and image processing was performed with MrC5/Axiovision software (Zeiss ...
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bioRxiv - Immunology 2021Quote: ... bacteria were stained with 2µg/µL of 4’,6-diamidino-2-phenylindole (DAPI) and mounted in ProLong Diamond Antifade (Life Technologies, Eugene, OR, USA; P36961). Image acquisition was performed using a Leica confocal microscope (Leica ...
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bioRxiv - Developmental Biology 2022Quote: ... Fixed hearts were embedded in paraffin and sections (4-6 μm thickness) were mounted onto Superfrost® Plus microscope slides (Thermo scientific #630-0950). Immunohistochemistry was carried out using standard procedures ...
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bioRxiv - Bioengineering 2022Quote: ... Rat bone marrow mononuclear cells were cultured at 4-6 million in a 10cm dish (Falcon) using differentiation media containing: DMEM (ThermoFisher Scientific, Burlington, ON, Canada), 20% HI FBS ...
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bioRxiv - Immunology 2023Quote: 1 × 106 NIKWT and NIKKO BMDMs were stimulated with 20ng/mL IL-4 or PBS for 6 hours before being lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific; Waltham, MA, USA). Cells were spun down and washed once with PBS before being flash frozen in liquid nitrogen ...
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bioRxiv - Immunology 2023Quote: 5×105 NIKWT and NIKKO BMDMs were stimulated with 20ng/mL IL-4 for 6 hours before being lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific; Waltham, MA, USA). Cells were spun down and washed once with PBS before total RNA was isolated from cells by Purelink™ RNA Mini Kit (Life Technologies) ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were stained with DAPI (4’,6-diamidino-2-phenylindole) and cover-slipped with ProLong Glass Antifade Mountant (P36984, Thermo Scientific, Waltham MA, USA). All images were acquired on a Keyence-BZ800 microscope ...
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bioRxiv - Microbiology 2019Quote: ... pellets were thawed at 37°C and then subjected to a single round of sonication on ice (Sonic Dismembranator 60, Fisher Scientific, setting 8, 5 seconds). Lysates were clarified by centrifugation at 20,000 rcf for 60 minutes at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... 6% Sodium bicarbonate solution (Gibco), 8% Fetal bovine serum (Sigma Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... TNF and IL-6 (Invitrogen) ELISA kits were used according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2020Quote: ... or QuantStudio 6 (Applied Biosystems) thermocycler ...
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bioRxiv - Cell Biology 2021Quote: ... 6′-diamidino-2-phenylindole (Invitrogen). All observations were performed on a Nikon E600 epifluorescence microscope ...
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bioRxiv - Microbiology 2021Quote: ... 6 μg/ml geneticin (Invitrogen) was added ...
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bioRxiv - Neuroscience 2019Quote: ... IL-6 (Invitrogen Carlsbad, CA), TNF-α (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... then 6 µl Lipofectamine (Invitrogen) were added ...
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bioRxiv - Cell Biology 2019Quote: ... 6 mM MgCl2 (Invitrogen, AM9530G), 1 mM each of dNTP’s (ThermoScientific ...
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bioRxiv - Neuroscience 2019Quote: ... hiPSCs were re-plated as clumps in Geltrex (Thermofisher)-coated 6-well plates (Thermofisher) in mTeSR1 (StemCell ...
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bioRxiv - Cancer Biology 2020Quote: ... TRAF-6 (Invitrogen-PA5-29622), and Cyclin D1 (Invitrogen-AHF0082) ...
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bioRxiv - Molecular Biology 2020Quote: ... IL-6 (Invitrogen, California, USA), TNF-α (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... 6 mM L-glutamine (Gibco), 0.1 mM MEM non-essential amino acids (Gibco) ...