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Citations for Thermo Fisher :
9301 - 9350 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... 7 μL of RNase I (Invitrogen, Cat. No. AM2249) was added to the clarified lysates and digestion was carried out for 1 hour at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... Viability was controlled using 7-Aminoactinomycine D marker (Invitrogen). Fluorescence intensity of CTV and antibodies was measured with the SP6800 cytometer (Sony ...
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bioRxiv - Genomics 2021Quote: ... The embryoid medium was aspirated and 15 to 20 embryoid bodies were plated per well in 6-well plates and cultured in 3 mL hematopoetic medium (2 mM GlutaMax, 1x Antibiotic-Antimycotic (Thermo Fisher Scientific; Cat. No. 15240062), 55 mM 2-mercaptoethanol (BioRad ...
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bioRxiv - Neuroscience 2023Quote: ... The medium was changed every day and cells were passaged at 70%-80% confluency every 5-7 days using PBSCa-/Mg- (Life Technologies; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 0,5 mM UltraPureTM EDTA (Gibco ...
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bioRxiv - Immunology 2019Quote: ... 1-2×106 keratinocytes were mixed 1:1 with autologous fibroblasts in 400μl MEM(Gibco; 11380037) containing 1% FBS ...
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bioRxiv - Immunology 2020Quote: ... 1-2×106 keratinocytes were mixed 1:1 with autologous fibroblasts in 400µl MEM (Gibco; 11380037) containing 1% FBS ...
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bioRxiv - Cell Biology 2021Quote: ... Stable cells were selected with 1.5 μg ml−1 puromycin (1 mg ml−1 stock; Invitrogen) 24 hours after replating ...
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bioRxiv - Microbiology 2022Quote: ... 1% (v/v) penicillin/streptomycin (10,000 U ml−1 penicillin, 10,000 μg ml−1 streptomycin; Gibco), 2 mM L-glutamine (Gibco ...
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bioRxiv - Cell Biology 2022Quote: 1 million cells per mL were loaded with 1 μM Indo-1 (Catalog No. I1223, Invitrogen) in 0.1 % pluronic-containing cell culture medium for 30 min at a 37° incubator with 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... diluted 1:10 with PBS and 1:1 with trypan blue stain (Invitrogen™, Cat. #T10282), and counted on the Countess II Automated Cell Counter (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: Embryos were first stained with a 1:1 mix of 1:50 Hoechst (ThermoFisher Scientific #62249) and 3:10,000 ice-cold DiI suspension (Invitrogen #D282 ...
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bioRxiv - Molecular Biology 2024Quote: ... M2 macrophage arginase-1 (Cell Signaling, 93668, 1:200 and neutrophils (Ly6G, Invitrogen 1960181, 1:100). For inflammatory and coagulation markers ...
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bioRxiv - Microbiology 2024Quote: ... and used in a 1:1:1 molar ratio Golden Gate assembly reaction with AarI (ThermoFisher) and T4 ligase (NEB ...
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bioRxiv - Microbiology 2024Quote: ... and used in a 1:1:1 molar ratio Golden Gate assembly reaction with AarI (ThermoFisher) and T4 ligase (NEB ...
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bioRxiv - Immunology 2024Quote: ... Perflubron was diluted 1:1 in 1 x Hank’s Balanced Salt Solution (Hy Clone, Fisher Scientific) prior to saturation with 100% O2 ...
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bioRxiv - Cell Biology 2023Quote: ... mouse Anti-ZO-1 (CatNo.339100, Invitrogen; 1:1000 for WB and 1:200 for IF), rabbit Anti- Bdkrb1 (CatNo.577292 ...
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bioRxiv - Immunology 2023Quote: ... diluted 1:10 with PBS and 1:1 with trypan blue stain (Invitrogen™, Cat. #T10282), and counted on the Countess II Automated Cell Counter (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... 1 million cells in 1 mL of DMEM AB+ with 1:10000 Hoechst 33342 (Invitrogen, H3570) were plated on a soft polyacrylamide gel in a coverslip cell chamber and incubated for 10 minutes at 37◦C or until the cells settled down ...
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bioRxiv - Bioengineering 2021Quote: ... 50 μg of pre-aliquoted Tandem Mass Tag 6-plex (TMT-6, Thermo Scientific) was resuspended in 20 μL anhydrous acetonitrile ...
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bioRxiv - Cancer Biology 2022Quote: ... Plasma IL-6 was measured by immunoassay (ProQuantum Human IL-6 Immunoassay Kit, ThermoFisher) and run on the ABI 7300 (Applied Biosystems ...
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bioRxiv - Bioengineering 2023Quote: FsC[Fe3+] was labelled by 6-carboxyfluorescein (6-FAM) (Thermo Fisher Scientific, Waltham, USA) to indicate the targeting of FsC[Fe3+] to C ...
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bioRxiv - Cell Biology 2022Quote: ... CD45-APC 1:200, Biolegend, CD117-APC 1:100, Biolegend; CD140a-APC 1:100, Thermo Fisher; CD29-APCe780, 1:250, Thermo Fisher, Biolegend) was prepared in the FACS buffer with 100 ng/ml DAPI ...
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bioRxiv - Immunology 2024Quote: ... Isolated CD8+ T cells were seeded onto treated BMDMs in a ratio of 1:1 resuspended in T cell media (RPMI 1640, 10% FBS, 1% A/A, 1% gentamicin (ThermoFisher Scientific cat#: 15710064) and incubated for 2 hours after which cells were treated with 1x Brefeldin A (BioLegend ...
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bioRxiv - Immunology 2019Quote: ... eBioscience Mouse IL-6 ELISA Ready-SET-Go! Kit (Fisher Scientific #50-112-8863; IL-1 beta Mouse Uncoated ELISA Kit (Thermo Fisher Scientific #88-7013-88).
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bioRxiv - Genetics 2022Quote: ... cells were maintained at 37°C and 6% CO2 in 25 mM (high) glucose DMEM (Thermo Fisher # 11995-065 with 1 mM sodium pyruvate; or Thermo Fisher # 11965-092 without pyruvate) supplemented with 10% v/v FBS (Thermo Fisher # 10437-036 ...
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bioRxiv - Neuroscience 2020Quote: ... Fluo-4 (Fluo 4 AM/Fluo 4 direct, Life Technologies) was used according to the manufacturer’s instructions and a previously published protocol (54) ...
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bioRxiv - Cell Biology 2020Quote: ... in PBS and incubated for 2-3 hr in secondary antibodies (conjugated to Alexa-488, Alexa-568, and Alexa 647, Life Technologies,1:500) at room temperature ...
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bioRxiv - Immunology 2021Quote: ... marinum via caudal vein injection and analysed for apoptotic cells at 1- and 3- dpi using the Click-iT™ Plus TUNEL Assay (Thermo Fisher, USA) according to the manufacturers protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... Rehydrated embryos were blocked with 10% BSA (in PBST) for 2-3 hours and incubated with anti-GFP antibody (1:1000; ThermoFisher Scientific, A-11120) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... They were then washed 3 times in PBS and incubated with a secondary anti-rabbit GFP antibody dissolved in PBST (1:1000, AF546, ThermoFisher, Waltham, MA, USA) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... After 3 × 15 min washes in 0.1 M PBS and 0.2% TritonX-100 the secondary antibodies were applied (in 0.1 M PBS, 0.2% TritonX-100 and 3% Normal Goat Serum): Alexa647 goat anti-rabbit (Invitrogen #A-21245, 1:1000 dilution). Slices were incubated in the secondary mix for 2 hr at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293T cells were seeded into 12-well plates and were transfected with 1.5 μg the RPL plasmid and 0.5 μg Cas13b gRNAs (NTC, U1-1, U1-2, U1-3) while ∼80% confluency using Lipofectamine 3000 (Thermo Fisher Scientific, Cat.# L3000015). For RIP experiments ...
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bioRxiv - Neuroscience 2019Quote: ... sections were washed in PBS (3 × 10 min) and incubated in goat anti-rabbit AlexaFluor 488 secondary antibody (1:500, ThermoFisher, cat# A-11008) for 2 hours in PBS on an orbital shaker ...
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bioRxiv - Genomics 2019Quote: We passed each cell suspension through a 35 µm filter (Falcon) and added 20 µl of 1 µM TO-PRO®-3 Iodide (Thermo Fisher Scientific) per ml to stain dead cells ...
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bioRxiv - Biochemistry 2019Quote: ... cDNA of 1 μLfrom 20 μL reaction volume was amplified by real-time quantitative PCR (Applied Biosystems Quant Studio 3, Thermo Fisher Scientific) with Perfecta SYBR Green Master Fast Mix (VWR ...
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bioRxiv - Molecular Biology 2020Quote: ... We then diluted these saturated cultures 1:20 in sterile water and then inoculated 3 µL into 96-well humidified plates (Nunc Edge Plates (Thermo Scientific, Waltham, MA)) with 150 µL of YPD or SD-CSM per well ...
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bioRxiv - Cell Biology 2021Quote: ... gels were incubated for 3 hr with a secondary antibody solution containing goat anti-rabbit Alexa Fluor 488 IgG (1:400, Thermo Fisher Scientific, A11008) and goat anti-mouse Alexa Fluor 568 IgG H +L (1:400 ...
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bioRxiv - Neuroscience 2020Quote: ... Tissues were then washed 3 times in PBS and incubated with appropriate fluorochrome secondary antibody (AlexaFluor® 488, AlexaFluor® 568, 1:400, Life Technologies), or biotinylated secondary antibody (Vector Labs ...
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bioRxiv - Neuroscience 2020Quote: ... Then the sections were washed 3x 10 min at RT in PBS and incubated on a shaker for 3 hours at RT with secondary reagents: streptavidin Alexa Fluor® 405 (1:200, Life Technologies (Invitrogen), S32351 ...
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bioRxiv - Cell Biology 2020Quote: ... Peptides were separated within a 25 min gradient (3 – 35% acetonitrile) on a monolith column (ProSwift™ RP-4H, 1 mm x 250 mm, Thermo Fisher Scientific) using an HPLC system (Agilent 1200 series ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were washed 3× for 10 min with PBS and incubated with the anti-rabbit secondary antibody (1:1000; Alexa Invitrogen, Walthan, United States), diluted in blocking solution ...
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bioRxiv - Biophysics 2022Quote: ... Cells were rinsed 3 times by 1X PBS and labeled with 500 μL of 1:100 anti-alpha Tubulin primary antibody (Thermo Fisher Scientific, 322500), 1:200 anti-Tomm20 primary antibody (Abcam ...
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bioRxiv - Microbiology 2021Quote: ... The phospholipolytic activity was measured according to 83 using a synthetic fluorescent substrate 1-palmitoyl-2-(10-pyrenyldecanoyl)-sn-glycero-3-phosphocholine (Molecular Probes, The Netherlands) and a Hitachi F-4000 spectrofluorimeter.
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed twice in PBS with 0.10% Tween-20 for 3 minutes each and treated with secondary antibodies conjugated with Alexa Fluor 488 (Invitrogen A-11039, 1:200) and/or Alexa Fluor 568 (Invitrogen A-11004 ...
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Synaptic Zn2+ potentiates the effects of cocaine on striatal dopamine neurotransmission and behaviorbioRxiv - Neuroscience 2021Quote: ... Tissue was washed with washing buffer 3 × 10 min at RT then incubated in secondary antibody (Alexa 488-Rabbit #A1134, Thermo Fisher (1:400), Topro (1:1200) ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then washed in blocking buffer at room temperature 3 x for 15 minutes each after which secondary antibodies were prepared using goat anti-rabbit (Invitrogen A10520; 1:500) and goat anti-rat (Invitrogen A11006 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then washed twice with PBS and incubated for 3 min at 37°C with 1 mg/ml Alexa Fluor™ 647-Dextran (Invitrogen, REF D22914) without preincubation or acid stripping ...
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bioRxiv - Neuroscience 2019Quote: ... They were then washed 3 times with PBS and incubated for 1h at room temperature in secondary antibody (1:1000; ThermoFisher, Cambridge, MA; R37117). They were then washed once with PBS ...
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bioRxiv - Genomics 2021Quote: ... For this QC-PCR 3 μl 1:10 diluted RNA was reverse transcribed using the TaqMan® MicroRNA Reverse Transcription kit (Thermo Fisher Scientific) in a multiplex reaction containing RT-primers for hsa-miR-486-5p ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed 3 times with WB before adding 100 µL 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo Fisher 34029) followed by incubation in the dark at room temperature with shaking (225 rpm ...