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Citations for Thermo Fisher :
8901 - 8950 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... G34W or K36M mutated were generated by transfection of plasmid encoding wildtype or mutated H3.3 and selection of clones in limiting dilution in medium supplemented with G418 (Life Technologies) starting 48 hours after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... AS ONE) were transfected with 1.5 μg expression plasmids using Lipofectamine LTX with Plus Reagent (15338-100, Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Constructed plasmids were linearised with NotI restriction enzyme and capped mRNA was synthesised using the SP6 mMessage Machine Kit (Ambion). Equal amounts of left and right TALEN mRNA (100pg each ...
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bioRxiv - Cell Biology 2022Quote: ... The above plasmids encoding viral structural proteins were co-transfected into HEK293T cells with an equimolar ratio using Lipofectamine 3000 (Invitrogen). The supernatant containing VLPs-EGFP were harvested 48 hours post transfection by centrifugation at 2000 × g for 15 minutes at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... 5’ and 3’ 400 bp homology regions flanking the Pkp230p guide were amplified from Pk A1-H.1 genomic DNA and inserted into a plasmid backbone containing a multiple cloning site (synthesized by Geneart, Thermofisher) via SacII/SpeI and BlpI/NcoI restriction sites ...
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bioRxiv - Cell Biology 2022Quote: The TNY1 cDNA coding sequences were cloned into the Gateway pDEST15-GST (glutathione S-transferase) plasmid using the procedures recommended by the manufacturer (Invitrogen) with oligos listed in Supplementary Table 1 ...
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bioRxiv - Immunology 2022Quote: ... sequence confirmed plasmid DNA was diluted in OptiMEM I serum-free medium and incubated for 20 minutes with ExpiFectamine 293 Reagent (Gibco). The DNA– ExpiFectamine 293 Reagent complexes were used to transfect Expi293F cell cultures in 96-deep-well blocks ...
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bioRxiv - Synthetic Biology 2022Quote: ... and peptide-CHIPΔTPR (50 ng) plasmids were transfected into cells as duplicates (2×104/well in a 96-well plate) with Lipofectamine 3000 (Invitrogen) in Opti-MEM (Gibco) ...
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bioRxiv - Plant Biology 2022Quote: ... Transformations with knock-out vectors were performed with plasmid-based vectors linearized with the appropriate restriction enzyme (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... and the vesicular stomatitis virus G (VSV-G)-expressing plasmid at a ratio of 7:7:1 (5 μg total amount of DNA) using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... van Hemert (Leiden University Medical Center) and viral RNA was obtained by in vitro transcription on linearized plasmids using T7 mMessage mMachine (Invitrogen). RNA was then transfected into BHK-21 to grow infectious virus ...
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bioRxiv - Physiology 2022Quote: ... HEK 293 were cultured in DMEM with 10% FBS and transfected with plasmid for 24 h using Lipofectamine 3000 transfection reagent (ThermoFisher). HEK 293 were cultured in 5 or 25 mM glucose ...
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bioRxiv - Microbiology 2022Quote: ... The promoters and the pRIT-GFP plasmid were amplified using the Phusion High-Fidelity DNA Polymerase (purchased from Thermo Scientific). All the PCR fragments were then purified using standard commercial silicon column (PCR cleanup kit ...
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bioRxiv - Immunology 2022Quote: ... HEK293T cells were transfected with the packaging plasmids psPAX2 and pCMV-VSV-G along with the transgene in pLenti-CMV-GFP-Puro using Lipofectamine 2000 (Invitrogen). pCMV-VSV-G was a gift from Bob Weinberg (Addgene plasmid # 8454 ...
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bioRxiv - Microbiology 2022Quote: ... the coding sequences of all open reading frames of TBEV Hypr were cloned and recombined into the pPC97 plasmid (Invitrogen) downstream of the DNA-binding domain of the Gal4 transcription factor (Gal4-DB ...
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bioRxiv - Microbiology 2022Quote: ... HEK-293T cells were transfected with plasmids encoding the WNV replicon and ZIKV structural genes at a 1:3 ratio by mass using Lipofectamine 3000 (Invitrogen), followed by incubation at 30°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Isogenic HeLa WT were transfected with a plasmid encoding sgRNAs using Lipofectamine 2000 according to manufacturer’s recommendations (Life Technologies #11668500). 24 hours post-transfection ...
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bioRxiv - Neuroscience 2022Quote: ... resuspended to 1mg/mL in H2O at a 3:1 (v/w) PEI:DNA ratio with 250 ng DNA per plasmid (750 ng total DNA) diluted in Opti-MEM (ThermoFisher Scientific) and added dropwise to cells ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293 and ATXN2-58 KI cells were stably transfected with GFP-LC3 or GFP-LC3-RFP plasmids and selected and maintained with G418 (Geneticin) (Invitrogen) or puromycin ...
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bioRxiv - Molecular Biology 2023Quote: ... Approximately 320 μg of the hSGLT2 plasmid and 160 μg of the MAP17 plasmid were premixed with 720 μL of FectoPRO reagent in 60 mL of Opti-MEM (Gibco) for 10–20 min before transfection ...
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bioRxiv - Immunology 2023Quote: ... without the insert and 3.7 µg of plasmid coding Furin into Freestyle 293F cells using a 293fectin Transfection Kit (Thermo Scientific) according to manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2024Quote: ... The 3 plasmids were shuttled into the backbone containing the cmcl2:GFP selection cassette (81) using the LR recombination (Invitrogen “Gateway LR Clonase II Plus Enzyme Mix” #12538-120) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Stable mixed population cell lines were established through G418 resistance (encoded by the plasmid vector (pcDNA3.1 neo+, Invitrogen, Paisley UK). Cells were maintained in Dulbecco’s modified Eagle’s medium (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... Reporter plasmid DNAs as well as control reporter plasmid DNAs with no repeats inserted were transfected in HEK293T cells in 24-well plates using Lipofectamine 2000 (ThermoFisher). 16-24 hours after transfection ...
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bioRxiv - Plant Biology 2022Quote: ... MMV N and P were amplified using MMV NF/NR and PF/PR primers from cDNAs synthesized from infected-plant RNA and subcloned into pTF binary plasmid between the double CaMV 35S promoter and nopaline synthase (NOS) terminator using Gateway Clonase enzyme mix (Invitrogen) (Figure 1a) ...
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bioRxiv - Molecular Biology 2022Quote: ... Stably expressing cell-lines were generated by transfecting 1 μg of the HaloTagged TF Piggybac construct together with 1 μg of the Super Piggybac transposase plasmid (gifted from the Tjian Lab) using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... two different sgRNA plasmids targeting both sides of the TDMD trigger were co-transfected into S2 cell by lipofectamine 3000 (Invitrogen) according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2022Quote: The cells were plated in 6-well plates at 2 × 105 per well and then cotransfected with 2.5 µg of plasmids using Lipofectamine LTX (ThermoFisher Scientific). After 48h ...
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bioRxiv - Genomics 2022Quote: ... For each transfection 1.6 ug of plasmid DNA has been incubated for 20 minutes with 4 µl of Lipofectamine 2000 transfection reagent (Invitrogen) in 0.2 ml of OptiMEM media (Gibco ...
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bioRxiv - Immunology 2022Quote: ... p.M694V MEFV was cloned into the GFP-expressing plasmid pINDUCER21 (45) under the control of a doxycycline-inducible promoter through the pENTR1A (Invitrogen) vector using a synthetic DNA fragment (GeneArt ...
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bioRxiv - Physiology 2022Quote: ... Cells were grown to 80% confluency prior to transfection or co-transfection with different plasmids for 24 hours using lipofectamine 2000 (ThermoFisher).
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bioRxiv - Molecular Biology 2022Quote: ... One thousand or five hundred nanograms of WT or mutated minigene plasmids were transfected into NIH 3T3 or A549 cells using Lipofectamine 3000 (Invitrogen) for 24 hours ...
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bioRxiv - Cell Biology 2022Quote: ... each cell well was transfected with 3 μg of plasmid DNA complexed with TurboFect transfection reagent (ThermoFisher, Grand Island, NY) for HEK293T cells ...
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bioRxiv - Cell Biology 2024Quote: ... we transfected Sox2se-CPG-TV-dTomato and Cdk1-Snrpn-dTomato plasmids into mouse iPSCs using Lipofectamine 2000 Transfection Reagent (Invitrogen) according to the provider’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... approximately 2 million U2OS cells were reverse transfected with 2 ug of the corresponding plasmid (GFP only, TIRR WT, TIRR RBM, and NES1, NES2, NES3 or NES4) using Lipofectamine LTX with PLUS reagent (ThermoFisher) in 6-well plates and then reseeded on 15 cm dishes the following day ...
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bioRxiv - Molecular Biology 2024Quote: ... U2OS cells were reverse transfected with 1 ug each of PAGFP-TIRR and mCherry-LaminB1-10 or LSM14A-mCherry plasmids of Lipofectamine LTX with PLUS reagent (ThermoFisher) in plastic 6-well plates and then reseeded on poly-L-lysine coated glass bottom 35mm plates ...
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bioRxiv - Microbiology 2024Quote: ... One fluorescent variant of aEPEC 1711-4 was obtained by transforming the wild-type strain with the recombinant plasmid pDP151 (Invitrogen), which encodes the fluorescent protein mCherry and confers resistance to ampicillin.
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bioRxiv - Cancer Biology 2024Quote: ... parental cells were transfected with All-in-One nickase plasmids via electroporation using the amaxa-nucleofector® II according to the manufacturer’s instructions (Thermofisher). 5-10 μg of the All-in-One plasmid were transfected into 106 cells ...
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bioRxiv - Biochemistry 2024Quote: ... the cDNA fragments in pENTR-D-TOPO were transferred to the modified pHAGE plasmids (6) using LR recombination (Invitrogen 56484).
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bioRxiv - Bioengineering 2024Quote: ... Cells were transfected at a concentration of 106 cells ml-1 with plasmid DNA (1 mg l−1) and 25 kDa linear polyethyleneimine (2 mg l−1) in OptiPRO SFM (Gibco). After 6 d of culture ...
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bioRxiv - Plant Biology 2023Quote: ... cells were transformed with a linearized plasmid encoding mito-Clover and a hygromycin-resistance cassette using the Max Efficiency Reagent (Invitrogen). Resistant clones to hygromycin were screened for Clover fluorescence using a M1000 Tecan plate reader (Excitation ...
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bioRxiv - Cancer Biology 2024Quote: ... The miR-539-5p mimic or NC was cotransfected with the wild-type or mutant plasmid using lipofectamine 3000 (Invitrogen). Luciferase intensity was recorded 48 h after transfection using the dual-luciferase reporter assay system (Promega ...
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bioRxiv - Cancer Biology 2024Quote: ... parental cells were seeded at 5 × 105 cells per well into a 6-well plate and transfected after 24 hours with 1 μg/μl plasmid (1 μl per well, 0.5 pg/μl final) using lipofectamine 3000 (Invitrogen, L3000015) (2.5 μl per well ...
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bioRxiv - Cell Biology 2024Quote: ... plasmids were complexed with lipofectamine 3000 using a 1 μg to 1.5 μl ratio in Opti-MEM medium (Gibco, 31985070) for 15 minutes at room temperature.
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bioRxiv - Cell Biology 2024Quote: ... a mixture of 1 μg of plasmid (Supplementary Table 2) in 100 μL of Opti-MEM was combined with 1.5 μL of TurboFect (Thermo Scientific), diluted in 100 μL of Opti-MEM ...
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bioRxiv - Cell Biology 2024Quote: ... Tandem RNAi (10 nM)/plasmid transfection for rescue experiments were performed at 48 h mark using Lipofectamine 3000 (Invitrogen L3000015) as follows.
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bioRxiv - Cell Biology 2024Quote: All eukaryotic expression plasmids for pUL71 and pUL71 homologues were generated in the backbone of pEF1/Myc-His C expression vector (Invitrogen) by cloning using restriction enzymes ...
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bioRxiv - Molecular Biology 2024Quote: ... we transfected keratinocytes in 24-well plates with 500ng/well circASH1L overexpressing plasmids (oe-cASH1L) or pcDNA3.1 vector (oe-ctrl) using LipofectamineTM 3000 reagent (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2024Quote: ... cells were transiently transfected at 50-60% confluency with plasmid expressing protein of interest (Table 1) and/or target specific siRNA using Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... about 5 million K562 cells were transfected with 5 μg luciferase reporter plasmid using Neon™ NxT Electroporation System (Invitrogen). Cells were fixed after 24 hours and ChIP performed as described above ...