Labshake search
Citations for Thermo Fisher :
9001 - 9050 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... The pENTR constructs were shuttled into modified pQCXIH-GW-GFP or pQCXIH- GW-Halo plasmids using the L/R reaction of the GatewayTM Cloning system (Invitrogen), according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2023Quote: ... and the FBPase promoter (Lloyd et al., 1991) were cloned into pDONR plasmids and then inserted into the binary pB7M24GW (Invitrogen) plasmid ...
-
bioRxiv - Bioengineering 2023Quote: ... pcDNA-uAb (500 ng) plasmids were transfected into cells as triplicates (3×105/well in a 24-well plate) with Lipofectamine 2000 (Invitrogen) in Opti-MEM (Gibco) ...
-
bioRxiv - Biochemistry 2023Quote: The cells were seeded in 6 well plate and treated the next day using 2 µg of plasmid and 4 µL of lipofectamine 3000 (Invitrogen) for 24 h or 10 nM of siRNA and 5 µL of RNAiMAX (Invitrogen ...
-
bioRxiv - Cell Biology 2023Quote: Hek 293T packaging cells were cultured and transfected with 12μg of equimolar plasmid DNA using the Lipofectamine 3000 reagent kit (Invitrogen, L3000001). 6% Lipofectamine 3000 in serum-free DMEM media was mixed 1:1 with diluted plasmid DNA (serum free media with 2% plasmid DNA and 4% P3000 reagent) ...
-
bioRxiv - Cell Biology 2023Quote: Bacteria was cultured overnight and plasmids were purified with a maxiprep kit according to manufacturer’s instructions (Thermo Fisher Scientific, A31217).
-
bioRxiv - Cell Biology 2023Quote: ... Bacteria was cultured overnight and plasmids were purified with a maxiprep kit according to manufacturer’s instructions (Thermo Fisher Scientific, A31217).
-
bioRxiv - Cell Biology 2023Quote: ... lipofectamine transfections were carried out by combining transfection reagent and DNA plasmid in OptiMEM media (Gibco Life Technologies, Carlsbad, CA), followed by an incubation period dictated by manufacturer protocols ...
-
bioRxiv - Plant Biology 2023Quote: ... Verified entry clones were used to transfer the CMV 2b sequences into pSITE (Chakrabarty et al., 2007) destination plasmids following incubation with LR clonase (Invitrogen) for 1 hour at 25°C ...
-
bioRxiv - Biochemistry 2023Quote: ... 7.1 μg of the PRKN library plasmid and 0.48 μg of pCAG-NLS-Bxb1 (17.5:1 molar ratio) were diluted with OptiMEM (Thermo Fisher Scientific) to a total volume of 710 μL in an Eppendorf tube ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells expressing extrachromosomal plasmids were transformed by electroporation and grown in appropriate antibiotic selection by addition of either 20 μg/mL hygromycin (Invitrogen) or 10 μg/mL G418 (Sigma) ...
-
bioRxiv - Cell Biology 2023Quote: ... lipofectamine transfections were carried out by combining transfection reagent and DNA plasmid in OptiMEM media (Gibco Life Technologies, Carlsbad, CA), followed by an incubation period dictated by manufacturer protocols ...
-
bioRxiv - Genetics 2023Quote: ... Briefly guide RNAs used to generate the 19 kb deletion and cloned in the pDG458 plasmid were in-vitro transcribed using the TranscriptAID T7 High Yield Transcription Kit (ThermoFisher) and purified using GeneJET RNA purification kit (ThermoFisher) ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... The next day cells were transfected with a mixture of 4 ug plasmid DNA and a 1:3 ratio of PEI (1mg/mL) in Opti-Mem (Gibco). Forty-eight hours later ...
-
bioRxiv - Genetics 2023Quote: ... 2016) and cloned into Gateway entry plasmids (pDONR 221 P4-P1R, pDONR 221, and pDONR 221 P2r-P39 (ThermoFisher Scientific)) ...
-
bioRxiv - Biophysics 2023Quote: ... Stable cell line is obtained upon plasmid transfection (epb-bsd-EIF1a-LAMP1eGFP) using Lipofec-tamine™ 2000 Transfection Reagent (ThermoFisher). The cells are then selected by Blasticidin (5 µg/mL ...
-
bioRxiv - Cancer Biology 2023Quote: ... The final inducible expression plasmids were generated in the pLenti-CMVtight-Hygro-DEST by the Gateway LR-Clonase II Enzyme Mix system (Invitrogen). All cDNA amplification and cloning steps were validated by DNA sequencing.
-
bioRxiv - Cell Biology 2023Quote: ... UGGT2-/- and UGGT1/2-/- cells were transfected with 1 µg Trop-2-pEYFP-N1 or Trop-2-Q118E-pEYFP-N1 plasmids using Lipofectamine 3000 DNA transfection reagent following the manufacturer’s instructions (Life Technologies). Mock-transfection using the pEYFP-N1 plasmid was used as a negative control.
-
bioRxiv - Cancer Biology 2023Quote: ... Annealed double stranded DNA were inserted into vector pL-CRISPR.EFS.GFP (addgene plasmid #57818) digested with restriction enzyme Esp3I (Thermo Fisher Scientific). Correct clones were confirmed by Sanger sequencing ...
-
bioRxiv - Neuroscience 2023Quote: ... Cell-specific promoter fragments were amplified from genomic DNA or preexisting plasmids and introduced into pENTR41 or pENTR 50-TOPO vectors (Invitrogen); CDS of interest were inserted into pDONR221[1-2] (Invitrogen) ...
-
bioRxiv - Genomics 2023Quote: ... as described below were diluted (5 µg of plasmid or 10 µM tracr-complexed synthetic guide RNAs) in OptiMEM (GIBCO). Lipofectamine 2000 (Invitrogen ...
-
bioRxiv - Molecular Biology 2023Quote: ... Template for modRNA synthesis was PCR amplified from linearised plasmid using Platinum Taq DNA Polymerase High Fidelity (Invitrogen, 11304-011) using manufacturer’s programme specifications and the following primers.
-
bioRxiv - Molecular Biology 2023Quote: ... Transfection complexes were prepared by adding 5 ug of plasmid DNA (pcDNA 3.4 TOPO) to 300 μl of optiMEM (Life Technologies), and 18 μl of Fugene HD (Promega ...
-
bioRxiv - Molecular Biology 2023Quote: ... The selection of OSCCas9+ cells with plasmid integrated into the genome was performed on the medium with puromycin (Gibco, #A1113803) in the concentration of 1 µg/ml.
-
bioRxiv - Molecular Biology 2023Quote: ... 50 or 90% confluent monolayer of cells (depending on the experiment) was transfected with various plasmid constructs or RNAs using Lipofectamine 2000 (Invitrogen) or Turbofectamine in Opti-MEM (Invitrogen) ...
-
bioRxiv - Neuroscience 2023Quote: ... or DIV12 (overexpression) primary hippocampal neurons were transfected with 1-2 μg plasmid DNA and 5 μL Lipofectamine 2000 (Invitrogen) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... The METTL16 plasmid DNA was transfected into cells using Lipofectamine 2000 according to the instructions of the manufacturer (ThermoFisher, 11668019).
-
bioRxiv - Plant Biology 2023Quote: The 6xHis-NBSNIK1 recombinant plasmid has been previously cloned in pDEST17 via LR-mediated recombination (Gateway Invitrogen Life Technologies, Inc). It harbors the NIK1 kinase domain fused to 6xHis under the control of an isopropyl-β-d-thiogalactopyranoside (IPTG ...
-
bioRxiv - Cell Biology 2023Quote: ... pEGFP-N1- hDEK plasmids encoding the mutated DEK versions were transfected into U2-OS DEK knockout cells using Lipofectamine 3000 reagent (Invitrogen). 3-9 days after transfection (depending on cell growth and viability ...
-
bioRxiv - Systems Biology 2023Quote: ... with 500 ng of donor plasmid and 250 ng of each sense and anti-sense plasmids using Lipofectamine LTX according to the manufacturer’s instructions (Life Technologies). The transfected cells were grown for 24 h before selection with fresh DMEM media supplemented with 3 μg/ml puromycin for another 48 h ...
-
bioRxiv - Biophysics 2023Quote: ... Adherent semiconfluent HEK293SG cells cultured in DMEM:F12 medium supplemented with 10% FBS were transfected in 6-well plates with 2 μg/well of plasmid DNA complexed with Lipofectamine 2000 reagent (Invitrogen). The cells were incubated for 48 hrs at 37 °C under 7% CO2 ...
-
bioRxiv - Biophysics 2023Quote: Plasmids for mosaic experiments such as Actin-GFP or Caldesmon-GFP were incubated with cells using Lipofectamin (Invitrogen, cat. 11668030). One day prior experiment ...
-
bioRxiv - Molecular Biology 2023Quote: ... HEK293T cells were seeded in 6-well plates and transfected with a total of 1 µg of plasmid DNA per well using Lipofectamine 2000 (Invitrogen) as transfecting reagent ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Libraries were prepared for long-read sequencing by digesting 2 µg of a level 3 plasmid library using Esp3I (Thermofisher) and purifying linearized fragments using a 0.5x volume of magnetic beads (Omega Bio-Tek ...
-
bioRxiv - Cell Biology 2023Quote: ... Lentivirus was generated according to the manufacturer’s specifications by co-transfecting HEK293-FT’s with a mixture of packaging plasmids (ViraPower Lentivirus Expression System; ThermoFisher #K497500). Briefly ...
-
bioRxiv - Genomics 2023Quote: ... Final plasmids were purified with phenol/chloroform (#A931I500 and #C/4920/15, Fisher Chemical) and concentration was determined using Qubit (Invitrogen).
-
bioRxiv - Plant Biology 2023Quote: The 6xHis-NBSNIK1 recombinant plasmid has been previously cloned in pDEST17 via LR-mediated recombination (Gateway Invitrogen Life Technologies, Inc). It harbors the NIK1 kinase domain fused to 6xHis under the control of an isopropyl-β-d-thiogalactopyranoside (IPTG ...
-
bioRxiv - Microbiology 2023Quote: ... DNA plasmid encapsulation efficiency (EE%) was quantified using a Quant-iT™ PicoGreen® dsDNA assay kit (Thermo Fisher Scientific) as described previously (23).
-
bioRxiv - Systems Biology 2023Quote: ... The lentiCRISPRv2 plasmid containing the start-stop target sequence was transiently transfected into HAP1 cells using Lipofectamine 3000 (Thermo Fisher) and incubated for 24 hours ...
-
bioRxiv - Molecular Biology 2023Quote: ... Sequences encoding KNL12026-2342 and ZWINT wild-type and mutants were cloned into a pCDNA5/FRT/TO-EGFP-IRES plasmid and co-transfected with pOG44 (Invitrogen), encoding the Flp recombinase ...
-
bioRxiv - Microbiology 2023Quote: ... In-vitro Transcription was performed using 1ug of linearised plasmid with MEGAscript® T7 Transcription Kit (Thermo Scientific, Cat # AM1334) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2023Quote: ... plasmids encoding for various SCTs were co-transfected with a plasmid encoding for an ER-retained BirA-ligase into FreeStyle 293-F cells (Invitrogen) using the 293-free transfection reagent (Merck ...
-
bioRxiv - Cell Biology 2023Quote: ... the COS-7 cells were transfected with plasmids encoding tubulin (3×mEmerald-ensconsin) and ER (mCherry-KDEL) by using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s protocol17 ...
-
bioRxiv - Cell Biology 2023Quote: ... PCR products of ame1 and okp1 point mutants (with C-terminal TAF tag and G418 marker) were amplified from the mutant plasmids using a high-fidelity DNA polymerase (SuperFi, Invitrogen) and transformed into the respective haploid ame1 and okp1 null mutants ...
-
bioRxiv - Bioengineering 2023Quote: Plasmids were transfected as a stoichiometric mixture (21 μg) in HEK293T/17 cells using Lipofectamine 2000 and Plus Reagent (Invitrogen) together with psPAX2 (15 μg ...
-
bioRxiv - Plant Biology 2023Quote: A 112 nt long product from the GFP sequence was PCR amplified from plasmid pB7WGF2 with two oligos pairs (A349/A347 and A346/A348) using Phusion polymerase (ThermoFisher). The PCR products were purified on gel ...
-
Zero-shot learning enables instant denoising and super-resolution in optical fluorescence microscopybioRxiv - Bioengineering 2023Quote: ... the plasmid coding for the spCas9 and the free PCR product containing the sgRNA targeting sequence using Lipofectamin 3000 (Invitrogen) according to the manufacturer’s instruction ...
-
bioRxiv - Cell Biology 2022Quote: ... in a 1:1 (w/w) ratio to plasmid DNA in serum-free Opti-minimal essential medium (Opti-MEM) (GIBCO). C2C12 myoblasts were transfected using TransfeX reagent (ATCC ...
-
bioRxiv - Cell Biology 2022Quote: ... Myc or FLAG-tagged procaspase-9 plasmid was prepared by cloning the procaspase-9 gene into a pcDNA-based vector (Invitrogen). Procaspase-9 with three mutations (Asp315Ala ...
-
bioRxiv - Cell Biology 2023Quote: ... Plasmids were then linearized using NotI restriction enzyme and mRNA was transcribed in vitro using Sp6 mMessage mMachine kit (ThermoFisher), following the manufacturer’s instructions ...