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Citations for Thermo Fisher :
8751 - 8800 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: Plasmid pCAG-CD5-S-ecto-6P-Spytag was transiently transfected into ExpiCHO cells using ExpiFectamine CHO transfection kit (Thermo Fisher). After 18-22 hr of transfection ...
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bioRxiv - Immunology 2020Quote: ... Plasmids encoding gp120 were transfected into Expi293F cells at a density of 2E6 cells/ml in Expi293 Expression Medium (ThermoFisher) using ExpiFectamine (ThermoFisher ...
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bioRxiv - Biochemistry 2021Quote: ... Both the LentiCRISPRv2-sgRNA plasmid and the ss donor oligonucleotides were transfected into D3 mouse ES cell line using lipofectamine 3000 (Invitrogen). Transfected cells were selected with puromycin (1.5 μg/ml ...
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bioRxiv - Biochemistry 2020Quote: ... 2020) were generated by PCR-amplifying 5’ UTR sequences from pRF plasmids using AccuPrime Pfx DNA Polymerase (Thermo, Invitrogen, 12344024). pSP73-4xS1m(MCS ...
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bioRxiv - Biochemistry 2020Quote: A 100 ml culture (1 × 106 cells/ml) of 293F cells were transfected with 100 µg of pCMV-FLAG-His6-NPC1L1-MLD plasmid using 293-fectin (Invitrogen) according to the manufacturer ...
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bioRxiv - Microbiology 2022Quote: The coding sequence of the kinase domain of EiIF2K-A (1313 bp) and EiIF2K-B (1397 bp) was synthesized and ligated into the pYES-NT/C plasmid (ThermoFisher, kind gift of Dr ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293T cells were plated in 10cm dishes at 80-90% confluency the day before transfection and transfected 16 hours later with 4 μg total DNA (2 μg of each plasmid) using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2022Quote: ... 150ng of donor plasmid was added and the total volume made up to 15uL with nuclease-free MQ H2O (Invitrogen). This mix was microinjected into the pronucleus of zygotes followed by oviduct transfer into pseudopregnant females ...
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bioRxiv - Genetics 2022Quote: ... Positive pX459-gRNA clones were confirmed by Sanger sequencing and further expanded using the PureLink HiPure Plasmid Midiprep Kit (Invitrogen). All donor single-stranded oligodeoxynucleotides (ssODNs ...
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bioRxiv - Microbiology 2022Quote: ... Transgenic GDPD:loxPint:HA:Neo-R parasites were then further transfected with the plasmid pSkipFlox (Birnbaum et al., 2017) and selected with 2 μg/ml blasticidin S (Invitrogen) for constitutive expression of the DiCre recombinase under the crt promoter ...
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bioRxiv - Microbiology 2022Quote: ... They were then co-transfected with 500 ng of IRF3-GFP and 500 ng of SARS-CoV-2 protein-expressing plasmid using Lipofectamine 2000 reagent (11668019, Invitrogen). 24 hours post-transfection ...
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bioRxiv - Systems Biology 2022Quote: ... The consistency of plasmid expression throughout different experiments was ensured by transfecting 80 ng of each plasmid per well of an 8-well Lab-Tek™ chambered cover glass slides (0.8 cm2 surface area, ThermoFisher) and the amount of plasmid was scaled up proportional to the culture-well surface area for other culture-plates used ...
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bioRxiv - Systems Biology 2022Quote: ... The PCR product was cloned into the HindIII and BamHI sites of pcDNA3.1(+) plasmid by using GeneArt™ Seamless Cloning and Assembly Enzyme Mix (Invitrogen). OMP25-HA was cloned into the HindIII and BamHI sites of the pSNAP-N1 plasmid ...
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bioRxiv - Systems Biology 2022Quote: ... grown on LB medium containing 100 µg/mL spectinomycin and extracted with the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific). Quality control was performed by restriction digest with NotI (Promega) ...
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bioRxiv - Genetics 2019Quote: ... A random 18-mer barcode was cloned into the resulting plasmid by restriction digest with Pfl23II (BsiWI) and XbaI (ThermoFisher). Further descriptions of the cloning scheme ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T were either stably transduced with lentiviruses or acutely transfected with expression plasmids using Lipofectamine 2000 (Invitrogen, Cat# 11668-019), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Expression plasmids for Omicron sublineage spike proteins were produced by assembling and cloning codon-optimized overlapping gene fragments (Thermo Fisher) into the pCDNA3.1/V5-HisTOPO vector (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... and transfected with pMT-GAL4 (Drosophila Genomics Resource Center) and pUAST plasmids containing YN and YC fusions using the calcium phosphate transfection kit (Invitrogen). 10 mg of each construct were used ...
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bioRxiv - Cell Biology 2022Quote: ... mRNA for transient expression of EGFP tagged fusion proteins were made by linearisation of plasmid DNA followed by mRNA synthesis and purification using either T7 or SP6 mMessage transcription kit (Ambion). For transient expression ...
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Cryo-EM structure of a eukaryotic zinc transporter at a low pH suggests its Zn2+-releasing mechanismbioRxiv - Biochemistry 2022Quote: The gene encoding the full-length wild-type ZnT8 from Xenopus tropicalis (xtZnT8, NCBI accession: NP_001011041.1) was synthesized by Genewiz (Suzhou, China) in a modified pPICZ plasmid (Thermo Fisher Scientific) containing a carboxy-terminal tag of Tobacco etch virus (TEV ...
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SARS-CoV2 associated secretion of nanoLuciferase reports on virus and Virus-Like Particle productionbioRxiv - Molecular Biology 2022Quote: HEK-293 cells were transfected with the indicated plasmids mixed at a 1:2 (µg DNA : µL transfection reagent) ratio with Lipofectamine2000 (Invitrogen) in OpitMEM (Gibco ...
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bioRxiv - Microbiology 2022Quote: ExpiCHO cells were transfected with equimolar amounts of pLM-M28 light chain and pLM-M28 heavy chain expressing plasmids according to the manufacturer’s protocol (Thermo Fisher). At 12 days post-transfection ...
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bioRxiv - Synthetic Biology 2019Quote: ... 100 ng/well of each amplicon or 400 ng/well of each plasmid was used) and 10 ng/well pRL-CMV control vector were cotransfected into cells with Lipofectamine 2000 (Invitrogen) reagent following a standard protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... we amplified the sgRNA expression cassette from pENTR-PpU6-sgRNA-L1L2 using primers (Supplemental Primer Table) containing different Multisite Gateway attachment sites (attB) and subsequently recombined with the Multisite Gateway pDONR221 plasmid set (Invitrogen) using a BP clonase reaction following the manufacturer’s recommendations.
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bioRxiv - Cell Biology 2019Quote: Plasmids encoding for left and right TALENs and the homologous recombination donor plasmid (1.5 µg each) were transfected using a Neon Transfection system (Life Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: C2C12 cells were electroporated (1650 V, 10 ms, 3 pulses) with 0.5 μg of PRMT7-FLAG plasmid using Neon transfection system (Life Technologies), following manufacturer instructions ...
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bioRxiv - Immunology 2019Quote: ... 2×106 HEK293T cells were transiently transfected with 20 μg Hem1-3xFLAG plasmid DNA using Lipofectamine 3000 reagent (Thermo Fisher) and cultured for 72 hours before harvest ...
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bioRxiv - Synthetic Biology 2019Quote: ... of DNA was prepared with specific combinations of plasmids and brought up to a volume of 100 µL using 0.15 M sodium chloride (NaCl, Fisher Scientific). Next ...
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bioRxiv - Microbiology 2019Quote: ... Eight μL of Cellfectin II reagent and 3 ng of each recombinant plasmid were diluted in 100 μL Grace’s medium (Thermofisher Scientific), respectively ...
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bioRxiv - Neuroscience 2019Quote: ... Partially overlapping complementary mutagenic primers were used to amplify the plasmid using Phusion® High-Fidelity DNA Polymerase (Thermo Scientific), then DpnI digestion was used to remove bacterially-derived template DNA ...
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bioRxiv - Cancer Biology 2019Quote: ... Lentiviral particles were generated by co-transfecting HEK293T cells with second generation packaging plasmids (psPAX2 and pMD2.G) using Lipofectamine 3000 (Invitrogen). Medium containing lentiviral particles was collected 48 h and 72 h after transfection ...
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bioRxiv - Developmental Biology 2019Quote: ... The Tol2 mRNA was transcribed from the pCS2FA-transposase plasmid (41) using the mMessage mMachine SP6 Transcription Kit (Thermo Fisher). Stable transgenic larvae were identified by out-crossing adults injected with the cdh1:cdh1-sfGFP or cdh1:cdh1-TagRFP BAC transgenes ...
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bioRxiv - Developmental Biology 2019Quote: ... NLS-GFP mRNA was synthesized from linearized NLS-GFP plasmid (Ariotti et al., 2015) using the mMessage mMachine Sp6 transcription kit (Invitrogen). Prior to injection ...
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bioRxiv - Microbiology 2019Quote: ... prepared minipreps from 2 ml of O/N cultivation at 37 °C using the GeneJET Plasmid Miniprep Kit (ThermoFisher Scientific), checked for the presence of insert using restriction digest and agarose gel electrophoresis ...
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bioRxiv - Biochemistry 2019Quote: ... 1.5 μg psPAX2 packaging plasmid and 500 ng pMD2.G envelope plasmid were co-transfected in HEK293 FT cells in 10-cm dishes using Lipofectamine (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... with 4 μg of a plasmid mixture (1:1:4 THR:RXR:TRE-Luc) in 200 μL Opti-MEM (Invitrogen, 11058-021) and added to the cells ...
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bioRxiv - Bioengineering 2020Quote: ... The sample was then analyzed in either 1% agarose gel (for GFP fragments amplified from the pEGFP-C1 plasmid) pre-stained with either SYBER Gold (Invitrogen), GelRed (Biotium Inc.) ...
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bioRxiv - Biophysics 2019Quote: ... the HEK-293T cells were transfected with a plasmid encoding full-length LRRK2(I2020T) fused with YFP at the N-terminus using a Lipofectamine 3000 transfection kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Colonies positive for the plasmid were selected on LB agar with 100 μg/ml ampicillin (Gibco from Thermo Fisher Scientific), 100 μM 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal ...
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bioRxiv - Cell Biology 2020Quote: ... Colonies positive for the plasmid were selected on LB agar with 100 μg/ml ampicillin (Gibco from Thermo Fisher Scientific), 100 μM 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal ...
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bioRxiv - Genetics 2021Quote: ... U2OS and U118-derived cell lines stably expressing GFP or GFP-ADAR2 plasmids 26 were grown in standard medium supplemented with 0.5 mg ml−1 G418 (Gibco, Invitrogen). Cells expressing RNWG and RNAG were grown in in standard medium supplemented with 0.5 mg ml−1 G418 (Gibco ...
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bioRxiv - Genetics 2021Quote: ... the PCR products from either primer pairs 6/7 or 9/10 with the plasmid vector pCR 2.1 (Thermo Fisher) followed by transformation of One Shot Top 10 chemically competent cells (Thermo Fisher ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... The FBW2 coding sequence as well as AGO1 NT-PAZ and AGO1 L2-CT were amplified by PCR using primers listed in Table S3 and used for gateway recombination using pDONR-Zeo plasmid (Invitrogen). Then ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: The 35S:FBW2 construct was generated by PCR amplification from the FBW2 cDNA with the oligonucleotide primers listed in Table S3 and used for gateway recombination using respectively the pDONR-Zeo plasmid (Invitrogen). Then ...
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bioRxiv - Plant Biology 2020Quote: Full-length cDNAs encoding SlG1-5 and PSY1-2 proteins without their stop codons were amplified by PCR and cloned via BP clonase into pDONR207 entry plasmid using Gateway (GW) technology (Invitrogen). Full-length sequences were then subcloned through an LR reaction into pGWB405 plasmid for subcellular localization assays ...
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bioRxiv - Synthetic Biology 2020Quote: ... The total amount of DNA for each transfection was kept constant by adding appropriate amounts of the control plasmid pcDNA3 (Invitrogen). 24h after transfection ...
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bioRxiv - Synthetic Biology 2020Quote: ... The total amount of DNA for each transfection was kept constant by adding appropriate amounts of the control plasmid pcDNA3 (Invitrogen).
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Suppressing STAT3 activity protects the endothelial barrier from VEGF-mediated vascular permeabilitybioRxiv - Molecular Biology 2020Quote: ... HUVEC seeded at 1.5 × 106 cells per well of a collagen-coated 6-well pate were transfected with plasmids using the Neon Transfection System (Invitrogen), harvested 48 hours post-transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... 300,000 cells in 6-well plate were transfected for 48 h with 2 µg of plasmid DNA using Lipofectamine 2 000 (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Pathology 2021Quote: ... The extracted pDONOR plasmid with the insertion was used to perform an LR Clonase reaction as instructed by the manufacturer (Invitrogen) to transfer the genomic region into the destination vector ...