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Citations for Thermo Fisher :
8551 - 8600 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Samples were then handled by the University of Colorado Anschutz Genomics and Microarray Core for microarray analysis using a Human Clariom D chip (902922; Applied Biosystems via ThermoFisher). This array has over 540,000 transcripts on it including mRNAs ...
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bioRxiv - Cancer Biology 2024Quote: RNA isolation and RT-qPCR for human tissue were performed as described previously (O’Donnell et al., 2023) using a hybrid TRIzol (Life Technologies 15596018) and RNeasy Plus Mini kit protocol after liquid nitrogen pulverization ...
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bioRxiv - Immunology 2024Quote: ... and growth factors using the multiplexed-bead assay Non-Human Primate Cytokine & Chemokine & Growth Factor 37-plex ProcartaPlex (Invitrogen, Life Technologies), following manufacturers’ instructions ...
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bioRxiv - Immunology 2024Quote: ... and growth factors using the multiplexed-bead assay Non-Human Primate Cytokine & Chemokine & Growth Factor 37-plex ProcartaPlex (Invitrogen, Life Technologies), following manufacturers’ instructions ...
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bioRxiv - Genomics 2024Quote: ... along with the N-terminal peptidase domain of human ACE2 was expressed using the Bac-to-Bac® baculovirus system (Invitrogen). The RBD (Residues Arg319-Phe541 ...
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bioRxiv - Cancer Biology 2021Quote: ... COS-1 cells were transiently transfected with either 5 or 10 μg of each of pCIneo-FOXA1-V5 and pCIneo-V5 plasmids using lipofectamine 3000 Reagent (Invitrogen).
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bioRxiv - Cancer Biology 2021Quote: ... and NHE9 plasmids (10 μg) were co-transfected with pLKO.1-shNHE9 in HEK293FT cells with lipofectamine 3000 (ThermoFisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... of with sgRNA (see Table S3 for plasmids and Table S4 for primers) using Lipofectamine LTX with Plus Reagent (A12621, Thermofisher) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: 50.000 N2A cells were seeded to 24-well plates overnight and double-transfected the following morning with 2.5 µg pMirGlo luciferase reporter constructs and pLenti-III overexpression plasmid DNA using Lipofectamine LTX (Thermo Scientific) reagent ...
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bioRxiv - Microbiology 2020Quote: ... These three amplicons contained unique BsiWI and BssHII sites at their extremities were first cloned in pCR2.1-TOPO plasmid (Invitrogen, USA) and Sanger sequenced ...
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bioRxiv - Cell Biology 2020Quote: ... transgenes were created with PCR from Krt5-Ppl-GFP Plasmids or Krt5-Ppl(sRT2)-GFP with SuperFi DNA Polymerase (Invitrogen). PCR products were gel extracted ...
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bioRxiv - Cell Biology 2020Quote: ... The chloramphenycol acetyl transferase (cat) gene was PCR amplified from the pLysS plasmid (Thermo Fisher Scientific, isolated from BL21(DE3)pLysS E ...
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bioRxiv - Cell Biology 2019Quote: Different lengths of the promoter regions of both CAP-D2 and CAP-D3 were cloned between the SalI and NotI restriction sites of the pEntr 1A plasmid (Invitrogen). The sequences were amplified from gDNA with the primer pairs D2-1156F/D2ProR for the Pro4 fragment ...
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bioRxiv - Immunology 2021Quote: ... The resulting plasmids were validated by sequencing and the mutated MEFV constructs were transferred into the GFP-expressing plasmid pINDUCER21 47 under the control of a doxycycline-inducible promoter using LR recombinase (Invitrogen). Lentiviral particles were produced in 293T cells using pMD2.G and psPAX2 (from Didier Trono ...
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bioRxiv - Immunology 2021Quote: ... cell line (PAOC/CD47p) was created via nucleofection of pAOC-SV40-hTERT with plasmid expressing Cas9 protein (GeneART CRISPR Nuclease Vector, Invitrogen) and guide RNA targeting GGTA-1 gene (aGal protein ...
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bioRxiv - Immunology 2021Quote: ... PAOC/CD47h4 and PAOC/CD47p/h4) (Reinhold et al., 1995) were created via nucleofection with plasmid expressing Cas9 protein (GeneART CRISPR Nuclease Vector, Invitrogen) and guide RNA targeting AAVS1 safe harbor site using Lonza 4D-Nucleofector ...
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bioRxiv - Genetics 2021Quote: ... plasmid DNA containing the requisite attB-GT and attB-GA sites flanking the transgenic donor DNAs are isolated using the Purelink HiPure Plasmid Filter Midiprep kit (Invitrogen) followed by extensive purification via phenol-chloroform extraction ...
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bioRxiv - Developmental Biology 2021Quote: ... These three plasmids were linearized with Not1/Kpn1 and used as templates to synthesize the capped mRNAs using mMessage Machine (Ambion). The RNAs were injected into single cell stage embryos as described previously (35 pg per embryo).
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bioRxiv - Genetics 2019Quote: ... For insertions MEL745a cells were transfected with one plasmid targeting the sequence surrounding the previously deleted enhancer and a double stranded repair template using Lipofectamine 3000 (Invitrogen) according to manufacturer instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... kcnk5b cDNA was isolated by RT-PCR from regenerating adult fin cDNA library and cloned into MCS region of pcDNA6-myc-6xHIS-tag plasmid (Invitrogen) or pBluescript harboring the hsp70 zebrafish promoter and GFP coding sequence surrounded by 2 miniTol2 sites ...
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bioRxiv - Immunology 2021Quote: ... the lentiviral vector (32 µg), the packaging plasmids (VSV-G: 16 µg, gag/pol: 24 µg) and DNAse-free water (Invitrogen) for a final volume of 2 mL ...
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bioRxiv - Microbiology 2019Quote: ... 1 μg of this plasmid was transfected into 5×105 SH-SY5Y cells with Lipofectamine LTX PLUS (1:1 ratio; Invitrogen) and selected for colonies with G418 (0.5 mg/ml) ...
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bioRxiv - Microbiology 2019Quote: ... 8 x 10^5 HEK-293T/17 cells pre-plated in a 6-well plate were co-transfected with a mass ratio of 1:3 replicon:C-prM-E plasmids using Lipofectamine 3000 (Cat# L3000-015; ThermoFisher Scientific). Four hours post-transfection ...
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bioRxiv - Molecular Biology 2021Quote: Madin-Darby canine kidney (MDCK) cells were grown on coverslips in 24-well plates and transiently transfected with DNA plasmids using Lipofectamine 2000 (Invitrogen). At 24 h after transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... or a construct without the nine MS2 stem loops and co-transfected with plasmids as indicated using Lipofectamine 2000 (Invitrogen).
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR was used to amplify an approximately 1 kbp upstream and downstream region surrounding each auxotrophy-causing mutation and the product was cloned into pCR8/GW/TOPO plasmid using the TA cloning kit (Invitrogen). Sequence identity was verified by DNA sequencing and the correct DNA fragment was then cloned into the BglII site of pUIC3 vector63 ...
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bioRxiv - Microbiology 2020Quote: ... The plasmid was double digested with BamH1 and Nhe1 to yield the gat1 fragment that was cloned into similarly digested pET15-TEVi plasmid (Invitrogen), resulting in the original 346 amino acid coding sequence of Gat1 extended at its N-terminus with a His6-tag and TEV protease cleavage site (MGSSHHHHHHSSGRENLYFQGH-) ...
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bioRxiv - Developmental Biology 2020Quote: ... the mutated plasmid served as a template to synthesize a PCR product containing a T7 promoter using Platinum Taq (Invitrogen). Custom primers (forward ...
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bioRxiv - Synthetic Biology 2022Quote: ... resuspended to 1mg/mL in H2O at a 3:1 (v/w) PEI:DNA ratio with 250 ng DNA per plasmid (750 ng total DNA) diluted in Opti-MEM (ThermoFisher Scientific) and added dropwise to cells.
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bioRxiv - Microbiology 2022Quote: ... The two pAcgp67-RBD (residues 333–530) plasmid with a C-terminal 8×His tag were transfected into Sf9 cells using Cellfectin II Reagent (Invitrogen) to produce the recombinant baculoviruses ...
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bioRxiv - Immunology 2022Quote: ... The following day cells were transfected with 2.5 µg of pCMV-TRAF2-HA and/or pCMV-IRE1α-FLAG plasmid DNA using 10 µl of Lipofectamine 2,000 (Invitrogen, 11668027) in Opti-MEM I Reduced Serum Medium (Gibco ...
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bioRxiv - Biophysics 2022Quote: NG108-15 cells were transiently transfected with 300 ng of each cDNA expression plasmids (see Supplementary Table 1) by using Lipofectamine 2000 Transfection Reagent (ThermoFisher) according to its handbook ...
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bioRxiv - Microbiology 2022Quote: ... cells seeded in a 6-well plate were transfected with 1 μg of pPRIME-FLuc plasmid using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: All expression plasmids in this study were constructed by Three-Fragment Multisite gateway (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) (Magnani et al. ...
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bioRxiv - Biochemistry 2020Quote: ... pcDNA3-myc-S or pcDNA3-S plasmids (500 ng DNA per ml of culture at 2 × 106 / ml) using Expifectamine (ThermoFisher). Cells were analyzed by flow cytometry 24 h post-transfection ...
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bioRxiv - Molecular Biology 2020Quote: ... Xist transgenes were cloned via PCR or recombineering into a custom RMCE-cargo vector and then electroporated along with a plasmid expressing Cre-recombinase into RMCE-competent cells using a Neon Transfection System (Invitrogen). Individual colonies were picked and genotyped ...
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bioRxiv - Molecular Biology 2020Quote: ... Wild type and catalytic mutant H672N RNase L plasmids cDNAs (the backbone is pcDNA5-FRT-TO with CMV promoter, Invitrogen) used in this study were a generous gift from Dr ...
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bioRxiv - Molecular Biology 2020Quote: ... were grown to 70-80% confluency and transfected with 40 μg plasmid DNA using Lipofectamine 3000 (cat. no. L3000-015, Invitrogen), following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Flp-In T-REx 293 cells were co-transfected with a hNAA40 L(dTIS>CTC) or hNAA40S expression construct and the Flp recombinase encoding pOG44 plasmid (Invitrogen) in a 1:9 ratio (in 6-well plates at 60-70% confluency ...
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bioRxiv - Molecular Biology 2019Quote: ... To construct the terminator-test plasmid a p.syn expression cassette (Anice Sabag-Daigle and Charles J. Daniels, in preparation) synthesised by lifetechnologiesTM (Thermofisher Scientific) was introduced via NotI/EcoRI into pTA231 (43) ...
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bioRxiv - Cell Biology 2022Quote: ... the LRRK1RCKW plasmid was used for the generation of recombinant baculoviruses according to bac-to-bac expression system protocols (Invitrogen).
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bioRxiv - Cell Biology 2022Quote: ... or 1 μg of Super8-FOPflash (8 mutant TCF-binding sites) reporter plasmids (kindly provided by Randall Moon, University of Washington, Seattle) using Lipofectamine 2000 (Invitrogen); a thymidine kinase-Renilla plasmid (0.1 μg ...
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bioRxiv - Microbiology 2022Quote: ... plasmid and insert (from gDNA) were amplified with Phusion U Hot Start DNA Polymerase (Thermo Fisher Scientific Inc., Waltham, USA) and ligated with USER™ Enzyme (New England Biolabs Inc. ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting plasmid was utilized for the generation of recombinant Baculoviruses according to the Bac-to-Bac expression system protocol (Invitrogen). Exponentially growing Sf9 cells (2E06 cells/mL in Lonza Insect-XPRESS medium ...
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bioRxiv - Microbiology 2020Quote: ... Vero cells stably expressing HA-BirA were generated by transfecting cells with the expression plasmid described below using Lipofectamine 2000 (Invitrogen). 48 h post-transfection ...
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bioRxiv - Cell Biology 2019Quote: ... the indicated primer pairs were mixed with 1ng of pAcGFP-C1-IGF2BP1 plasmid in 1X Phusion High-Fidelity DNA Polymerase (F530L, ThermoFisher) mix at a final volume of 50 µl ...
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bioRxiv - Biochemistry 2019Quote: ... 30×106 cells were transfected with 15 μg of TERT plasmid (3xFLAG- or 3xFLAG-HaloTag-TERT) and 60 μg of pSUPER-hTR using 150 μl of Lipofectamine 2000 (Invitrogen) in a total volume of 7.5 ml of OPTI-MEM (Gibco) ...
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bioRxiv - Genetics 2019Quote: ... Transfection was performed using 2 μg plasmid DNA and 3 μL Lipofectamine in 150 μL Opti-MEM I (ThermoFisher 31985062). At collection ...
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bioRxiv - Immunology 2019Quote: ... These plasmids were then used as templates to synthesize sgRNAs by in vitro transcription using the MEGA-shortscript T7 Kit (ThermoFisher). Cas9 mRNA was transcribed in vitro using the mMESSAGE mMACHINE T7 Ultra Kit (ThermoFisher ...
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bioRxiv - Cancer Biology 2020Quote: ... Digoxigenin-labelled anti-sense RNA probes were transcribed from linearized plasmid DNA using a MEGAscript(tm) kit (Ambion, Life Technologies).