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Citations for Thermo Fisher :
801 - 850 of 10000+ citations for Zinc Finger Protein 50 ZNF50 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... and SuperBlockTM blocking buffer (Thermo Fisher Scientific, USA) for 30 minutes for blocking excess binding sites ...
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bioRxiv - Molecular Biology 2024Quote: ... using undiluted StartingBlockTM (TBS) Blocking buffer (Thermo Scientific). After washing ...
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bioRxiv - Developmental Biology 2023Quote: ... After blocking with 10% FBS (Thermo Fisher Scientific) in PBS for 1 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Avidin/Biotin Blocking Kit (Invitrogen, Carlsbad, CA, USA). After blocking with 10% normal goat serum (Vector Laboratories ...
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bioRxiv - Immunology 2024Quote: ... Samples were blocked with ELISA blocking diluent (Invitrogen) for 2 hr at 37°C then washed 3X ...
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bioRxiv - Cancer Biology 2021Quote: ... and Keratinocyte Media 50/50 (Gibco) containing 2% FBS ...
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bioRxiv - Developmental Biology 2022Quote: ... Histoclear (Fisher Scientific, #50-329-50) (3×1 hour) ...
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bioRxiv - Systems Biology 2021Quote: ... total peptide concentration in each sample was determined using the Pierce™ Quantitative Fluorometric Peptide Assay (Thermo Fisher Scientific) to ensure that the same total peptide amount across samples 1-17 and 28-35 (excluding samples 18-27 ...
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bioRxiv - Plant Biology 2021Quote: ... The peptide concentration of the reconstituted solution was determined by using Pierce quantitative colorimetric peptide assay (Thermo Scientific, USA).
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bioRxiv - Biophysics 2022Quote: ... and the modified peptides were used for immunoaffinity purification according to manufacturer’s instructions (SulfoLink Immobilization Kit for Peptides, ThermoFisher). 72 µL of PAS beads was incubated overnight with 100 µg purified SMC1 antibody and 100 µg purified SMC3 antibody ...
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bioRxiv - Systems Biology 2023Quote: ... peptides were quantified by BCA assay and 150 μg of peptide per sample were labeled with TMTPro reagents (ThermoFisher) for 2 hrs at room temperature ...
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bioRxiv - Microbiology 2022Quote: The maleimide labeling of the HR2 peptides was performed by mixing 100 µM peptide and 2.5 mM fluorescein-5-maleimide (ThermoFisher) in degassed buffer (50 mM sodium phosphate ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants containing the obtained peptides were purified using Pierce™ Peptide Desalting Spin Columns (Thermo Scientific, Bartlesville, OK, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Phosphorylated peptides were enriched from 1mg of total peptides using High-Select Fe-NTA Phosphopeptide Enrichment Kit (Thermo Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... and peptide quantitation was performed using the Pierce™ Quantitative Peptide Assays & Standards (Thermo Fisher Scientific, Waltham, United States).
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bioRxiv - Evolutionary Biology 2023Quote: ... and peptides were purified using HiPPR detergent removal kit and Pierce peptide desalting spin columns (both Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were resuspended in running solvent and quantitated using a Pierce Colorimetric Peptide Quantitation Assay (Thermo Fisher Scientific, 23275).
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bioRxiv - Biochemistry 2024Quote: ... Peptides were resuspended in running solvent and quantitated using a Pierce Colorimetric Peptide Quantitation Assay (Thermo Fisher Scientific, 23275). Peptides were separated and analyzed as described above ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were probed with primary antibodies in SuperBlock T20 (TBS) Blocking Buffer and fluorophore-conjugated secondary antibodies in SuperBlock (TBS) Blocking Buffer (Thermo Scientific), with 3 washes in SuperBlock T20 (TBS ...
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bioRxiv - Plant Biology 2022Quote: Coding sequences of mature proteins without signal peptide were amplified with primers listed in Table S1 and cloned into expression vector pETSUMO (Thermo Fisher Scientific, Massachusetts, USA). Correctness of the resulting constructs pETSUMO-ChElp2 and pETSUMO-Vd2LysM were confirmed by DNA sequencing and introduced into E ...
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bioRxiv - Immunology 2022Quote: ... and the QI9 peptide (QYIKWPWYI, residues 1208-1216 of the SARS-CoV-2 spike protein) maintained in RPMI 1640 medium (Thermo Fisher Scientific, Cat# 11875101) containing 10% FBS and 30 U/ml recombinant human IL-2 (Peprotec ...
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bioRxiv - Pathology 2022Quote: ... The immunoprecipitants were incubated with 50 µl of pre-washed protein A/G agarose bead (Thermo Fisher, 20421) for 4 h at 4°C ...
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bioRxiv - Plant Biology 2019Quote: ... 10 μL of anti-GFP antibody (MBL-598) and 50 μL of Dynabeads Protein G (Thermo Fisher Scientific) were used ...
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bioRxiv - Microbiology 2021Quote: ... 10–20 μg total protein was supplemented with 50 mM DTT and 4X LDS sample buffer (Life Technologies). Samples were heated to 70°C for 10 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts (50 μg) of proteins were loaded and fractionated on 4–12% NuPAGE Bis-Tris Gels (Invitrogen). Protein were transferred to PVDF membranes (Millipore ...
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bioRxiv - Microbiology 2020Quote: ... 1×106 cells were lysed in 50 μL of M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific). Subsequently ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein samples (50 μg) were resolved by one-dimensional denaturing 12 % Novex Trisglycine gels (Invitrogen, Burlington, ON, Canada) and transferred onto polyvinylidene difluoride membranes (PVDF ...
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bioRxiv - Molecular Biology 2020Quote: ... The nuclei extract (input) was used for immunoprecipitation with 50 μL of Dynabeads-Protein A (Thermo Fisher Scientific) and 1 μg of anti-GFP antibodies (Abcam ab290 ...
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bioRxiv - Developmental Biology 2022Quote: ... Beads-protein complexes were then heated (50°C, 10min) in NuPAGE™ LDS Sample Buffer (4X) (Invitrogen, NP0008). Samples were magnetized prior supernatants collection and incubated (70°C ...
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bioRxiv - Neuroscience 2022Quote: ... 50 μg/ml of GPC3core protein was mixed with Alexa568-conjugated anti-hIgG antibody (Thermo Fisher Scientific, A21090) in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... 50 μg protein per sample was used to perform mouse Granzyme B Platinum ELISA (Thermo Fisher Scientific #BMS6029) and the protocol was followed as per the product manual.
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bioRxiv - Genomics 2023Quote: ... The chromatin was incubated overnight at 4°C with 50 μl Protein A magnetic beads (Dynabeads, Thermo Fisher) pre-bound with 2.5 μl α-CENH3 (gift of Prof ...
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bioRxiv - Bioengineering 2023Quote: ... 50 µL protein samples were transferred into an uncoated flat bottom 96–well plate (Cat # 12565501, Fisher Scientific) and absorbance at 350 nm (for turbidity ...
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bioRxiv - Developmental Biology 2024Quote: ... two gRNA with 50 pg each and 200pg of TrueCut Cas9 Protein v2 (Life Technologies, Cat. No. A36498) were co-injected into embryo at two-cell stage ...
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bioRxiv - Cell Biology 2023Quote: ... Bound proteins were alkylated by addition of 3.3 µL of 50 mM Iodoacetamide (IAA, Thermo Fisher Scientific # A39271) to each sample ...
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bioRxiv - Cell Biology 2021Quote: ... Fixed cell layers were washed gently with PBS once before addition of endogenous biotin blocking solutions (Endogenous Biotin Blocking kit, Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... cells were blocked for one hour with mouse-on-mouse blocking reagent (ReadyProbes™ Mouse-on-Mouse IgG Blocking Reagent, 1:30 dilution, ThermoFisher, R37621) between antibody staining procedures.
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bioRxiv - Genomics 2020Quote: ... Peptides were resuspended in 0.1% TFA 2% ACN and quantified using Pierce™ Quantitative Fluorometric Peptide Assay (Thermo Fisher Scientific). Equal portions of all samples were mixed together to make a reference sample to be run multiple times for chromatogram library runs.
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bioRxiv - Cancer Biology 2022Quote: ... samples were resuspended in 0.1% FA and peptides were quantified by Pierce™ Quantitative Fluorometric Peptide Assay (Thermo Fisher Scientific). 500 ng of peptides of each sample were analyzed by Liquid Chromatography-Mass Spectrometry (LC-MS).
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bioRxiv - Microbiology 2022Quote: Peptides were fractionated into eight fractions using the high pH reversed-phase peptide fractionation kit (Pierce™ Thermo Fisher Scientific) according to the manufacturer’s instructions and using self-made columns as previously described48 ...
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bioRxiv - Cancer Biology 2021Quote: Two-dimensional fractionation—Peptides were fractionated using a Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific, 84868) according to the manufacturer’s instructions with slight modifications ...
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bioRxiv - Physiology 2021Quote: ... Peptide concentration was measured with a colorimetric assay (Pierce Quantitative Colorimetric Peptide Assay kit, Thermo Fisher Scientific, Rockford, IL, USA).
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bioRxiv - Immunology 2020Quote: ... the HLA-A*02:01-restricted KRASwt (KLVVVGAGGV) and KRASG12D (KLVVVGADGV) aa5-14 peptides were synthesized (Peptide 2.0) and analyzed on an Orbitrap Fusion (Thermo Scientific) mass spectrometer (MS ...
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bioRxiv - Immunology 2022Quote: ... The Hc gene with a N-terminal gp67 signal peptide and Lc gene with a N-terminal honeybee melittin signal peptide were subcloned into pFastBac Dual vector (Invitrogen) containing two multiple cloning sites ...
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bioRxiv - Immunology 2022Quote: ... MBP peptide NPVVHFFKNIVTPR and influenza hemagglutinin PRFVKQNTLRLAT peptide were labeled with Alexa Fluor 488 (Alexa488) tetrafluorophenyl ester (Invitrogen, Carlsbad, CA) and used as probe peptides for DP4.1 ...
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bioRxiv - Biochemistry 2023Quote: ... Tryptic peptide mixtures including the stable-isotope labelled standard peptides were analysed on a nano-HPLC (Easy nLC, Thermo Scientific) coupled to an Orbitrap mass spectrometer (LTQ-Orbitrap ...
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bioRxiv - Biochemistry 2023Quote: ... HM and CM peptides resulting from the previous step were desalted using peptide desalting spin columns (Thermofisher Scientific, Brisbane, Australia), according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... solubility of 19F peptide library was estimated by fluorescence spectroscopy using Pierce Quantitative Fluorometric Peptide Assay (Thermo Fisher Scientific, USA), and 0.5 mg/ml solution in DMSO was used as a standard ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... The mixture of TMT-labeled peptides was acidified and fractionated using a high pH reversed-phase peptide fractionation kit (ThermoFisher) according to the manufacturer’s recommendation ...
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bioRxiv - Immunology 2023Quote: Phosphorylated peptides were enriched from 10uG total peptides using a High-Select Fe-NTA Phosphopeptide Enrichment Kit (Thermo Scientific A32992), according to the manufacturer’s instructions ...