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Citations for Thermo Fisher :
901 - 950 of 10000+ citations for Zinc Finger Protein 50 ZNF50 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... This step was followed by incubation with 35 μl protein A/G agarose plus (50% slurry) (ThermoFisher Scientific 20421) for 6 hours ...
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bioRxiv - Biochemistry 2022Quote: ... bait domains and control proteins (5-7.5 μg of protein per well in 50 μL PBS) were immobilised in a 384-well MaxiSorb plate (NUNC) o.n ...
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bioRxiv - Cell Biology 2022Quote: ... medium was replaced to low serum RPMI (RPMI-1640 supplemented with 1% HS and 1% FBS) with 50 ng/ml NGF 2.5S Native Mouse Protein (Gibco) to differentiate cells for desired time ...
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bioRxiv - Cell Biology 2019Quote: ... product of in vitro SUMOylation was incubated with 50 μL of anti-V5 coupled protein G magnetic dynabeads (Thermofisher) in Binding buffer A (25 mM HEPES pH 7.5 ...
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bioRxiv - Cancer Biology 2019Quote: ... Equal amounts of protein (30-50 µg) were fractionated on 4-12% gradient SDS-polyacrylamide gel electrophoresis gel (Invitrogen) and transferred to PVDF membrane (Roche) ...
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bioRxiv - Cell Biology 2019Quote: ... Protein (50 mM HEPES, pH 7.4, 150 mM NaCl) was quantified using NanoDrop™ 2000 spectrophotometer (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2020Quote: ... 50-75 μg of proteins were loaded on Pre-cast Native PAGE 3-12% Bis-Tris gels (Invitrogen, #BN2011BX10) at 150 V and 4°C for 4 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... A volume corresponding to 1.5 mg protein extract was incubated overnight with 50 uL Dynabeads (MyOne Streptavidin C1; Invitrogen). Protein-bound beads were collected and washed once with Strep-biotin wash buffer (50 mM Tris-HCL pH 8 ...
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bioRxiv - Cancer Biology 2023Quote: ... 50 μg of protein were resolved using a NuPAGE (4– 12%) Bis-Tris gel (Thermo-Fisher Scientific, Cat. # NP0322PK2). After the run ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... The lysates were then cooled on ice and combined with 50 μL of washed Pierce Protein A Agarose (ThermoFisher). The mixture was rotated at 4°C for 30 minutes and centrifuged at 3,000 g for 1 minute ...
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bioRxiv - Cancer Biology 2023Quote: ... aliquots from each sample containing 50 µg of protein were separated on Novex WedgeWell 10% Tris-Glycine gel (Invitrogen) and transferred to nitrocellulose membrane with iBlot 2 Dry Blotting System (Invitrogen).The blots were subsequently blocked for 1 h with 5% milk powder in Tris-buffered saline (TBS)/Tween 20 at RT and then probed overnight (at 4 °C ...
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bioRxiv - Genetics 2023Quote: Total protein was isolated from approximately 50 mg of snap-frozen distal-colon tissue using Cell Extraction Buffer (Invitrogen) containing Halt protease and phosphatase inhibitors (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: IgG was purified from 50 µl of plasma using the Pierce Protein G Spin Plate (Thermo Fisher catalog #45204). N-glycans were released using peptide-N-glycosidase F (PNGase F ...
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bioRxiv - Microbiology 2023Quote: ... cells were rinsed again thrice with 1×PBS before an overnight blocking stage at 4°C in 1×SEA BLOCK blocking buffer (Thermo Fisher Scientific, Poland).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... 50:50 with 1:200 N2 supplement (Invitrogen), 1:100 B27 supplement lacking Vitamin A (Invitrogen) ...
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bioRxiv - Neuroscience 2019Quote: ... 50:50 with 1:200 N2 supplement (Invitrogen), 1:100 B27 supplement lacking vitamin A (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... in 50:50 DMEM:F12 (31966 and 31765, Gibco) and plated on individual wells of 8 well glass bottom dishes (Ibidi ...
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bioRxiv - Cell Biology 2024Quote: ... MHCII (1:50; Fisher Scientific; 50-112-9377), and F4/80 (1:50 ...
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bioRxiv - Developmental Biology 2021Quote: Phospho-peptides were fractionated using alkaline reversed phase chromatography (Pierce™ High pH Reversed Phase Peptide Fractionation Kit, Thermo Scientific #84868) into 12 fractions using increasing concentrations of acetonitrile from 10%-80% in basic binding buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... All ten vials of samples were combined and approximately 100 μg peptide digests from this mix were fractionated using Pierce High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific) according to instructions from manufacturer ...
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bioRxiv - Biochemistry 2020Quote: ... samples were briefly centrifuged at 13,000 g and peptide-containing supernatant was used for further analysis Peptides were fractionated into eight fractions using High-pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher). Peptide fractions were dried in SpeedVac (Eppendorf ...
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bioRxiv - Cancer Biology 2021Quote: ... and remaining ~1.1 mg of multiplexed peptides were used for phospho-peptide enrichment by High-Select Fe-NTA Phosphopeptide enrichment kit (Thermo Fisher) after manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... TMT-labeled peptides were pre-fractionated with the basic pH reversed-phase C18 peptide fractionation kit following manufacturer’s manual (Thermo Fisher, 84868). Typically ...
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bioRxiv - Biochemistry 2019Quote: ... peptide concentration and primary amine reactivity were quantified using the Pierce quantitative fluorometric peptide assay (Thermo Fisher Scientific; cat. no. 23290) and Pierce quantitative colorimetric peptide assay (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... were raised in rabbits by immunization with keyhole limpet hemocyanin-conjugated peptides and affinity-purified by columns conjugated with the peptides (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: The phosphopeptide enrichment step flow through and washes were dried down and approximately 120 µg peptides were fractionated using the TMT fractionation protocol of Pierce high pH basic reversed-phase peptide fractionation kit (Thermo Fisher Scientific™ Cat no 84858 ...
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bioRxiv - Molecular Biology 2023Quote: ... 300 µL (90 µg) of reconstituted mixture of labeled peptides was fractionated using Pierce High pH Reversed-Phase Peptide Fractionation kit (84868, Thermo Fisher) according to the manufacturer’s protocol for TMT-labeled peptides ...
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bioRxiv - Biochemistry 2023Quote: The RC9 peptides with the sequence GGKGMGFGL was prepared using solid-state peptide synthesis with Fluorenylmethyloxycarbonyl (FMOC)-based chemistry by Life Technologies, Inc and purified by reversed-phase high pressure liquid chromatography (HPLC ...
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bioRxiv - Cell Biology 2023Quote: ... 50 mM Tris pH 7.5) followed by elution in 100 µl TBS + 150 ng/µL competitor peptide (Pierce 3x DYKDDDDK Peptide, Thermo Fisher Scientific) at 37 °C for 30 min.
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bioRxiv - Cell Biology 2023Quote: ... Dried peptides were resuspended in DI water and then quantified using Pierce Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific, Rockford, USA).
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bioRxiv - Plant Biology 2024Quote: ... Labeled peptides were separated into 6 fractions with Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (Thermofisher, Cat. no. 84868) and dried in a Speed Vac at 45 °C for 3.5 hours ...
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bioRxiv - Cell Biology 2024Quote: ... The deuterium exchange levels were determined by subtracting the centroid mass of undeuterated peptide from the centroid mass of deuterated peptide using HDExaminer (Version PD1.4, Thermo-Fisher Scientific, USA).
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bioRxiv - Plant Biology 2019Quote: ... Phospho-peptides were enriched from the remaining sample (900 µg) using High-Select Fe-NTA Phospho-peptide Enrichment Kit (A32992, Thermo Fisher Scientific). The TMT labeled samples were analyzed on a Fusion Lumos mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... peptides were fractionated into a final eight fractions using a Pierce High pH reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific, 84868), according to the manufacturer’s instructions.
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bioRxiv - Genomics 2022Quote: ... A portion of the peptides from the samples were pooled and fractionated using a Pierce High pH Reversed-Phase (HPRP) Peptide Fractionation Kit (Thermo Fisher Scientific) to generate a spectral library ...
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bioRxiv - Microbiology 2022Quote: Peptide concentrations were measured for fraction two of each sample using the Quantitative Fluorometric Peptide Assay kit (Pierce™ Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... An aliquot of the tryptic digests was used to determine the concentration of the peptide mixtures by using Pierce™ Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... The combined isobaric-labeled peptide solution was fractionated into eight fractions per manufacturer’s instructions using high pH reversed-phase peptide fractionation columns (ThermoFisher Scientific, Cat# PI84868). All eight peptide solutions were dried in a speed vac ...
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bioRxiv - Biochemistry 2023Quote: ... Mixed peptide samples were prepared by 9ul per sample and separated using the High pH Reversed-Phase Peptide Isolation Kit (Thermo Fisher Scientific) as directed ...
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bioRxiv - Neuroscience 2023Quote: ... The fractions were dissolved with 0.1% formic acid and peptide concentration was determined with Quantitative Colorimetric Peptide Assay (ThermoFisher Scientific, cat# 23275).
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bioRxiv - Cell Biology 2023Quote: ... Those peptides were divided into several fractions using the Pierce High pH Reversed-Phase Peptide Fractionation kit (Thermo Fisher Scientific, Waltham, MA) according to the manufacturer protocol ...
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bioRxiv - Immunology 2023Quote: ... The combined flowthrough and washes were dried down in a speed vac to completion for subsequent bench-top fractionation of non-phospho peptides by alkaline reversed phase chromatography (PierceTM High pH Reversed Phase Peptide Fractionation Kit, Thermo Scientific #84868) with a modified elution scheme as described in81 and MS analysis.
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bioRxiv - Neuroscience 2023Quote: ... The TMT labeled peptide pools were separated into eight fractions with the Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific), evaporated ...
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bioRxiv - Molecular Biology 2024Quote: ... Pooled TMT-labelled peptides were cleaned and fractionated using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific, 84868) according to manufacturer’s instruction for TMT experiments ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos were incubated in blocking buffer (10% Goat Serum (Invitrogen) in PBS-Triton ...
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bioRxiv - Developmental Biology 2022Quote: ... Alexa Fluor 647 Phalloidin (Invitrogen; 1:200 in blocking buffer) was applied to sections before mounting ...
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bioRxiv - Evolutionary Biology 2022Quote: ... diluted 1:300 in blocking buffer (REF A11012, Invitrogen, USA). Embryos were washed in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... followed by blocking in PBS Superblock (ThermoFisher Scientific, Cat. #37515) containing 4% normal goat serum (Jackson Laboratories ...
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bioRxiv - Genomics 2021Quote: ... 200 μL SuperBlock™ (TBS.) Blocking Buffer – Blotting (Invitrogen,37537) was added to each well and incubated for 2 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... After blocking with casein in phosphate buffered saline (PBS) (ThermoFisher) for 1 h at room temperature (RT) ...