Labshake search
Citations for Thermo Fisher :
1001 - 1050 of 10000+ citations for Zinc Finger Protein 50 ZNF50 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... 300 µL (90 µg) of reconstituted mixture of labeled peptides was fractionated using Pierce High pH Reversed-Phase Peptide Fractionation kit (84868, Thermo Fisher) according to the manufacturer’s protocol for TMT-labeled peptides ...
-
bioRxiv - Biochemistry 2023Quote: The RC9 peptides with the sequence GGKGMGFGL was prepared using solid-state peptide synthesis with Fluorenylmethyloxycarbonyl (FMOC)-based chemistry by Life Technologies, Inc and purified by reversed-phase high pressure liquid chromatography (HPLC ...
-
bioRxiv - Cell Biology 2023Quote: ... 50 mM Tris pH 7.5) followed by elution in 100 µl TBS + 150 ng/µL competitor peptide (Pierce 3x DYKDDDDK Peptide, Thermo Fisher Scientific) at 37 °C for 30 min.
-
bioRxiv - Cell Biology 2023Quote: ... Dried peptides were resuspended in DI water and then quantified using Pierce Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific, Rockford, USA).
-
bioRxiv - Plant Biology 2024Quote: ... Labeled peptides were separated into 6 fractions with Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (Thermofisher, Cat. no. 84868) and dried in a Speed Vac at 45 °C for 3.5 hours ...
-
bioRxiv - Neuroscience 2024Quote: ... were added to the endogenous peptide mixture and peptides were dried in a centrifugal Savant SpeedVac (Thermo Fisher Scientific, Waltham, USA). Dried peptides were used either for K-GG peptide enrichment or phospho-peptide enrichment as previously described in method sections K-ε-GG peptide enrichment for PRM analysis and phosphopeptide enrichment for PRM analysis ...
-
bioRxiv - Molecular Biology 2024Quote: ... Equal amounts of TMT-labeled peptides were pooled and fractionated into 8 fractions using a High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific) according to the manufacturer’s instructions.
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... 50:50 with 1:200 N2 supplement (Invitrogen), 1:100 B27 supplement lacking Vitamin A (Invitrogen) ...
-
bioRxiv - Neuroscience 2019Quote: ... 50:50 with 1:200 N2 supplement (Invitrogen), 1:100 B27 supplement lacking vitamin A (Invitrogen ...
-
bioRxiv - Developmental Biology 2020Quote: ... in 50:50 DMEM:F12 (31966 and 31765, Gibco) and plated on individual wells of 8 well glass bottom dishes (Ibidi ...
-
bioRxiv - Cell Biology 2024Quote: ... MHCII (1:50; Fisher Scientific; 50-112-9377), and F4/80 (1:50 ...
-
bioRxiv - Microbiology 2023Quote: ... cells were rinsed again thrice with 1×PBS before an overnight blocking stage at 4°C in 1×SEA BLOCK blocking buffer (Thermo Fisher Scientific, Poland).
-
bioRxiv - Plant Biology 2019Quote: ... Phospho-peptides were enriched from the remaining sample (900 µg) using High-Select Fe-NTA Phospho-peptide Enrichment Kit (A32992, Thermo Fisher Scientific). The TMT labeled samples were analyzed on a Fusion Lumos mass spectrometer (Thermo Fisher Scientific) ...
-
bioRxiv - Biochemistry 2022Quote: ... peptides were fractionated into a final eight fractions using a Pierce High pH reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific, 84868), according to the manufacturer’s instructions.
-
bioRxiv - Genomics 2022Quote: ... A portion of the peptides from the samples were pooled and fractionated using a Pierce High pH Reversed-Phase (HPRP) Peptide Fractionation Kit (Thermo Fisher Scientific) to generate a spectral library ...
-
bioRxiv - Microbiology 2022Quote: Peptide concentrations were measured for fraction two of each sample using the Quantitative Fluorometric Peptide Assay kit (Pierce™ Thermo Fisher Scientific) according to the manufacturer’s instructions.
-
bioRxiv - Microbiology 2020Quote: ... An aliquot of the tryptic digests was used to determine the concentration of the peptide mixtures by using Pierce™ Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2024Quote: ... Pooled TMT-labelled peptides were cleaned and fractionated using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific, 84868) according to manufacturer’s instruction for TMT experiments ...
-
bioRxiv - Neuroscience 2023Quote: ... The combined isobaric-labeled peptide solution was fractionated into eight fractions per manufacturer’s instructions using high pH reversed-phase peptide fractionation columns (ThermoFisher Scientific, Cat# PI84868). All eight peptide solutions were dried in a speed vac ...
-
bioRxiv - Biochemistry 2023Quote: ... Mixed peptide samples were prepared by 9ul per sample and separated using the High pH Reversed-Phase Peptide Isolation Kit (Thermo Fisher Scientific) as directed ...
-
bioRxiv - Neuroscience 2023Quote: ... The fractions were dissolved with 0.1% formic acid and peptide concentration was determined with Quantitative Colorimetric Peptide Assay (ThermoFisher Scientific, cat# 23275).
-
bioRxiv - Cell Biology 2023Quote: ... Those peptides were divided into several fractions using the Pierce High pH Reversed-Phase Peptide Fractionation kit (Thermo Fisher Scientific, Waltham, MA) according to the manufacturer protocol ...
-
bioRxiv - Immunology 2023Quote: ... The combined flowthrough and washes were dried down in a speed vac to completion for subsequent bench-top fractionation of non-phospho peptides by alkaline reversed phase chromatography (PierceTM High pH Reversed Phase Peptide Fractionation Kit, Thermo Scientific #84868) with a modified elution scheme as described in81 and MS analysis.
-
bioRxiv - Neuroscience 2023Quote: ... The TMT labeled peptide pools were separated into eight fractions with the Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific), evaporated ...
-
A conserved germline-specific Dsn1 alternative splice isoform supports oocyte and embryo developmentbioRxiv - Cell Biology 2024Quote: ... Pooled TMT-labelled peptides were cleaned and fractionated using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific, 84868) according to manufacturer’s instruction for TMT experiments ...
-
bioRxiv - Molecular Biology 2020Quote: ... Bound R-loops were recovered by addition of 50 μl pre-blocked dynabeads protein A magnetic beads (Thermo Fisher Scientific) followed by two washes and elution in an EDTA/SDS-containing buffer ...
-
bioRxiv - Biochemistry 2020Quote: ... The beads were washed 3 times then subjected for protein elution with 50 units of AcTEV protease (Invitrogen, 12575-015) in PBS buffer by 8 hr incubation in 4 °C on a rotator.
-
bioRxiv - Cancer Biology 2021Quote: ... 200-500 μg of nuclear lysates were incubated with 2 μg of specific antibodies and 50 μg of Dynabeads Protein G (Invitrogen). After overnight incubation on a rotator at 4°C ...
-
bioRxiv - Cancer Biology 2020Quote: ... 50-100 µg of protein was resolved over SDS-PAGE gels using 10% NuPAGE Bis-Tris precast gels (Life Technologies) and were then transferred onto polyvinylidene difluoride membranes (IPVH00010 ...
-
bioRxiv - Biochemistry 2021Quote: Cleared lysates from WB5 lysis buffer were adjusted to the same protein concentration before incubating them with 1/50 (vbeads/vlysate) equilibrated NeutrAvidin-agarose beads (ThermoFisher) over-night at RT ...
-
bioRxiv - Biochemistry 2022Quote: ... Biotinylated samples (1.2 mg of protein) were incubated with pre-washed Neutravidin agarose beads (200 µL, 50% slurry, Thermo Scientific) in 500 µL of PBS containing 1% SDS for 120 min ...
-
bioRxiv - Cancer Biology 2022Quote: ... RIPA extracts (50 µg of protein) were treated in NuPage lithium dodecyl sulfate (LDS) sample buffer (4X) (Thermo Fisher Scientific) supplemented with 50 mM dithiothreitol (DTT ...
-
bioRxiv - Molecular Biology 2020Quote: ... TAP purification was carried out as previously described 48 with 2 mg of protein extract and 50 μl of Dynabeads Pan Mouse IgG (Invitrogen) per sample ...
-
bioRxiv - Cell Biology 2019Quote: ... Equal amount of protein extract (50-100 µg) was resolved on Bolt™ 4-12% Bis-Tris Plus Gels (Invitrogen) and transferred onto PVDF membrane ...
-
bioRxiv - Cancer Biology 2024Quote: ... 200–500 μg of nuclear lysates were incubated with 2 μg of specific antibodies and 50 μg of Dynabeads Protein G (Invitrogen). Following an overnight incubation on a rotator at 4 °C ...
-
bioRxiv - Bioengineering 2023Quote: ... Fluorescence intensity as a function of pH was determined by dispensing 2 μL of the protein solution into 50 μL of the desired pH buffer in triplicate into a 384-well clear-bottomed plate (Nunc) and measured in a Safire2 plate reader (Tecan) ...
-
bioRxiv - Molecular Biology 2024Quote: ... whole cell or nuclear extracts were incubated with 2.5 µg of anti-FLAG antibody bound to 50 µl Dynabeads Protein G (Invitrogen #10003D) overnight at 4°C with gentle rotation ...
-
bioRxiv - Cell Biology 2024Quote: ... A total of 5 μg of GFP antibody that was pre-mixed in a 50 uL volume of Dynabeads protein A (Invitrogen) was added to each sonicated chromatin sample with 1% Triton X-100 and incubated overnight at 4°C ...
-
bioRxiv - Microbiology 2024Quote: ... Lysates were incubated with 5 μL of anti-SAG1 mAb DG52 and 50 μL of Dynabead protein G magnetic beads (Invitrogen) at 4 ℃ for 2 hr ...
-
bioRxiv - Plant Biology 2022Quote: ... a total of 1.75 mg of protein was used with 50 uL of Pierce™ Streptavidin Magnetic Beads (88816; Thermofisher) equilibrated with lysis buffer ...
-
bioRxiv - Developmental Biology 2023Quote: ... 50 µg of protein lysate per lane was then run on a NuPage 4-12% Bis-Tris gel (Thermo Fisher) at 120 V ...
-
bioRxiv - Cell Biology 2023Quote: ... Cleared lysates from WB5 lysis buffer were adjusted to the same protein concentration before incubating them with 1/50 (volbeads/vollysate) equilibrated NeutrAvidin-agarose beads (ThermoFisher) over-night at RT ...
-
bioRxiv - Immunology 2022Quote: ... the proteins were buffer exchanged into 20 mM HEPES pH 7.5 + 50 mM NaCl using Zeba Spin desalting columns (Thermo Scientific). Then 10 mM ATP ...
-
bioRxiv - Microbiology 2023Quote: ... N (75 fmol) and L (25 fmol) proteins and the T7 RNA polymerase (50 fmol) using lipofectamine 3000 (Invitrogen™) for 3 h at 37°C ...
-
bioRxiv - Molecular Biology 2023Quote: Recombinant Rpb4/Rpb7-6His protein (5 μg) was incubated in a 50 μL slurry of HisPur Cobalt Resin (Thermo Scientific) in binding buffer (20 mM Tris [pH 7.5] and 50 mM NaCl ...
-
bioRxiv - Cancer Biology 2023Quote: ... 30-50 µg of the protein was loaded on an 4-12% gel (NuPAGE Bis-Tris precast gels, Life Technologies) and transferred to an activated polyvinylidene difluoride membrane ...
-
bioRxiv - Developmental Biology 2024Quote: ... Precipitated protein pellets were resuspended in 70 μL of 50 mM HEPES pH 8.7 and labeled with TMTpro reagent (ThermoFisher Scientific) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... was used to measure protein concentration of each lysate and 50 μg of total protein of each was run on precast Bis-Tris 4–12% gradient gels (Invitrogen) and protein subsequently transferred to polyvinylidene fluoride membrane (Amersham) ...
-
bioRxiv - Neuroscience 2024Quote: Tissue lysis and protein extraction was completed by adding 25 µL of 5x RIPA buffer (50 mM Tris, pH 8.0 [Thermo Scientific, J75825-A4] ...
-
bioRxiv - Molecular Biology 2024Quote: ... Nuclear extracts were quantified with Bradford and 2mg of lysate was mixed with 6 µg of antibody and 50 µL of Protein G magnetic beads (Dynabeads, Invitrogen) in 500 µl of buffer C (300 mM NaCl ...