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Citations for Thermo Fisher :
651 - 700 of 10000+ citations for Zinc Finger Protein 50 ZNF50 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... with 5 μg primary antibody and 50 uL Pierce Protein G magnetic beads (Thermo Scientific). The primary antibodies used were rabbit anti-EMX2 (HPA065294 ...
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bioRxiv - Immunology 2020Quote: ... An additional 50 µL of 5× eBioscience Cell Stimulation Cocktail with protein transporter inhibitor (ThermoFisher) was added and cells were treated for an additional 4 hr at 37 °C.
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bioRxiv - Cell Biology 2019Quote: ... and 50 μl suspension (1.5 mg of beads) of Protein G-conjugated Dynabeads (Life Technologies) for 2 h at 4°C ...
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bioRxiv - Cell Biology 2022Quote: Protein samples (30-50 μg) were electrophoresed using NuPAGE 4-12% Bis Tris (Invitrogen, NP0321) or 3-8% Tris Acetate (Invitrogen ...
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bioRxiv - Genomics 2022Quote: ... protein A/G magnetic beads (50 µl for one gram of tissue; Thermo Fisher Scientific) were added to sheared chromatin and incubated at 4°C for 1 hour with rotation ...
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bioRxiv - Cancer Biology 2019Quote: ... Lysate (50–100 μg protein) was resolved on NuPAGE (4–12%) Bis-Tris gels (Invitrogen) and transferred to nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... For IP of hnRNP-C and RALY 50 μl of Protein G Dynabeads (Thermo Fisher) per sample were washed 3x in IP buffer (50 mM HEPES pH 7.4 ...
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bioRxiv - Immunology 2021Quote: ... Proteins were extracted from 50 million cells using the MEM-PER extraction kit (Thermo Fisher). Soluble proteins (1.5 ml ...
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bioRxiv - Biochemistry 2022Quote: ... The protein sample was crosslinked with 50 μM BS3 (bis(sulfosuccinimidyl)suberate) (Thermo Fisher Scientific) for 30 min at 37 degrees Celsius and 500 rpm ...
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bioRxiv - Genomics 2022Quote: ... was added to the soluble fraction and incubated with 50 μl Protein A Dynabeads (Invitrogen) bound to 5 μg of BRD4 antibody (Bethyl A301-985A50 ...
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bioRxiv - Microbiology 2022Quote: ... The cell lysate-Ab was then added to 50 μl of protein G Dynabeads (Invitrogen) for 6 hrs on a rotator at 40C ...
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bioRxiv - Neuroscience 2022Quote: ... The 50 ng/μl synthesized sgRNAs and 100 ng/μl TrueCut Cas9 protein v2 (Invitrogen) were premixed in Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... protein A/G magnetic beads (50 µl for one gram of tissue; Thermo Fisher Scientific) were added to sheared chromatin and incubated at 4°C for 1 hour with rotation ...
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bioRxiv - Neuroscience 2023Quote: ... A volume of 50 µL per sample of Protein G Dynabeads (#10003D; Thermo Fisher Scientific) was washed three times in 1-mL ice-cold low-salt wash buffer (50 mM Tris ...
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bioRxiv - Microbiology 2023Quote: ... The cell lysates were then combined with 50 µl of Protein G Dynabeads (ThermoFisher, 10004D) equilibrated lysate buffer B and re-incubated at 4°C for 30 min with rotation ...
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bioRxiv - Genomics 2023Quote: ... We used 50 µl of protein-G-coated magnetic beads (from Thermo Fisher Scientific #10004D) per sample to pull down the anti-BrdU antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... A mix of Protein A/G Dynabeads (50 µl of each per sample, Life Technologies) were coupled to 10 µg of rabbit anti-GFP antibody (Abcam ab290) ...
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bioRxiv - Cell Biology 2023Quote: ... 500 µl of lysates were incubated with 50 µl of Sepharose-beads-Protein-G (Invitrogen) previously incubated with 4 µl anti-HA antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... Bound proteins were eluted with 50 µL of 1xLDS sample buler (Thermo Fisher Scientific, NP0007) + 100 mM beta-mercaptoethanol at 80 °C for 10 min and run on 3-8% Tris-acetate gels (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Purified RNA was fragmented to a mode length of ∼200 nucleotides using zinc ion-mediated fragmentation (Ambion AM870, 70 °C for 4 minutes). Fragmented RNA was purified using miRNeasy spin columns (miRNeasy mini kit ...
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bioRxiv - Molecular Biology 2022Quote: ... Target peptides for each of these proteins were purchased in stable isotope labelled synthetic peptides (SIS) form in crude quality from Thermo Scientific. The C-terminal lysine or arginine in the SIS peptides were replaced by isotope labelled lysine (13C6 ...
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bioRxiv - Immunology 2022Quote: The encapsulation efficiency of the peptide into the NLC formulation was determined via the CBQCA protein quantitation kit (Thermo Fisher Scientific). Three separate NLC peptide formulations were created using the optimized formula previously determined in the DOE ...
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bioRxiv - Genomics 2020Quote: ... Flag peptide and small size of non-specific proteins were removed by 20K Slide-A-Lyzer dialysis cassette (ThermoFisher Scientific, 66003) with 1L binding buffer (PBS ...
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bioRxiv - Genetics 2021Quote: ... by rotating at room temperature for 1h and washed 3x with extraction buffer and protein was eluted 3x with 300 ul of 1 mg/mL HA peptide (ThermoFisher, 26184) in 1x TBS ...
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bioRxiv - Genomics 2021Quote: ... and 10 µg proteins were proteolysed with trypsin and peptides analysed by quantitative LC-MSMS on a Q Exactive HF (Thermo Fisher) by a data-independent acquisition approach as described earlier86 ...
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bioRxiv - Microbiology 2021Quote: ... Immunoprecipitated proteins were eluted from beads with 0.2 ml PDE storage buffer containing 1 mg/ml HA-peptide (Thermo Fisher Scientific) with mixing at 37°C for 30 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... The latter was performed in the National Laboratory of Research and Services in Peptides and Proteins using an LCQ DUO ESI ion trap (Thermo Finnigan) or QExactive Orbitrap (Thermo Scientific) spectrometers.
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bioRxiv - Biophysics 2021Quote: ... SARS-CoV-2 ORF10 protein (full lenth, crude, Mass spectrometry data of crude peptides is included in supplementary information) was purchased from Thermo Scientific, USA.
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bioRxiv - Microbiology 2022Quote: The raw LC-MS/MS data was analyzed against the Uniprot Gallid alphaherpesvirus 2 database (taxon 10390; 1300 sequences) using the Byonic peptide search algorithm (Protein Metrics) integrated into Proteome Discoverer 2.4 (Thermo Fisher Scientific). Optimal main search settings were initially determined with Byonic Preview (Protein Metrics ...
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bioRxiv - Cell Biology 2023Quote: The processing of BioID raw LCMS data for peptide and protein identification and quantification was performed with Proteome Discoverer v2.3 (Thermo Fisher Scientific). Raw mass spectra were filtered ...
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bioRxiv - Cell Biology 2023Quote: The processing of whole-cell protein quantitation raw LCMS data for peptide and protein identification and quantification was performed with Proteome Discoverer (PD v3.0 (Thermo Fisher Scientific) using a SPS MS3 reporter ion-based quantification workflow ...
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bioRxiv - Biochemistry 2023Quote: ... Interaction of His-fused proteins with other purified proteins (e.g. GST-PKAC) or synthetic peptides was studied by pulldown using Ni-NTA affinity resin (HisPur, Thermo scientific # 88221). GST-fused proteins were pulled down on glutathione-Sepharose beads (Amersham Biosciences) ...
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bioRxiv - Cancer Biology 2024Quote: ... Peptide concentration was calculated based on the peak area of 100 ng of Pierce HeLa protein digestion standard (Thermo Fisher Scientific). Afterwards the peptide solution was frozen at −70°C before further processing.
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bioRxiv - Biochemistry 2024Quote: ... Pierce™ Quantitative Colorimetric Peptide Assay (23275) and Pierce™ Rapid Gold BCA Protein Assay Kit (A53227) were ordered from ThermoFisher Scientific (Waltham ...
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bioRxiv - Biochemistry 2024Quote: ... was used as a reference protein database and peptide spectra matches were performed using the SEQUEST algorithm within Proteome Discoverer v.2.1 (Thermo Fisher Scientific) with a fragment tolerance of 0.6 Da and parent tolerance of 10 ppm ...
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bioRxiv - Bioengineering 2019Quote: ... tissues were wash 3 times with PBS and incubate with protein blocking solution that contain 0.5% Triton X-100 (Fisher Scientific, Hampton, NH). Then tissues were incubated in 1:250 diluted primary antibody (CD31 (PECAM-1 ...
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bioRxiv - Microbiology 2024Quote: ... coli type I fimbria or FliC (diluted 1:10 000 in protein-free tris-buffered saline (TBS) blocking solution (Fisher Scientific, France)) for 1 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Analytical separation of peptides was done on an Acclaim® PepMap RSLC column (75 µm × 50 cm, C18, 3 µm, 100 Å; Thermo Fisher Scientific) running a water-acetonitrile gradient at a flow rate of 300 nl/min ...
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bioRxiv - Genetics 2021Quote: ... The separated peptides were loaded onto a C18 column (50 cm × 75 μm) connected to an EASY-nLC™ 1200 system (Thermo Fisher Scientific, USA) and separated with a linear gradient of buffer B (80% acetonitrile and 0.1% formic acid ...
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bioRxiv - Neuroscience 2020Quote: ... Total protein lysates (15-50 µg) were subjected to SDS-PAGE using NuPAGE 4-12% Bis-Tris Midi Protein Gels (Invitrogen, Carlbad, MA, USA) and subsequently transferred to Polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2019Quote: ... Approximately 2μg peptide from each fraction was injected into an Acclaim peptide trap column (Thermo Fisher) via the Dionex RSLCnano autosampler ...
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bioRxiv - Cell Biology 2021Quote: ... 100pmol biotinylated peptides (Peptide 2.0) were bound to 8μl streptavidin M-280 Dynabeads (Thermo Fisher Scientific) for 30mins in PBS at room temperature with agitation ...
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bioRxiv - Genomics 2022Quote: ... Concentration of the stock H4K16ac peptide was analyzed by Pierce Quantitative Fluorometric Peptide Assay (Thermofisher Scientific). Spectra were collected with [15N-BD ...
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bioRxiv - Genomics 2021Quote: ... The concentration of eluted peptides was determined by Pierce™ Quantitative Fluorometric Peptide Assay (Thermo Scientific). The peptides were dissolved in 0.1% [vol/vol] TFA and 2% [vol/vol] acetonitrile (ACN) ...
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bioRxiv - Biochemistry 2021Quote: Peptide yields for optimisation were determined using the Pierce™ Quantitative Fluorometric Peptide Assay (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... The peptide concentrations were further determined by Quantitative Fluorometric Peptide Assay (Thermo Scientific™ Pierce™). Finally ...
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bioRxiv - Zoology 2022Quote: ... The redissolved peptides were measured using a colorimetric peptide assay kit (Thermo Fisher Scientific, MA, USA) and transferred to a hydrophilic-coated low adsorption vial (ProteoSave vial ...
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bioRxiv - Molecular Biology 2024Quote: ... Each fraction (∼4.5 mg peptides) was used for peptide enrichment with either M280 streptavidin beads (Invitrogen), MyOne streptavidin beads (Invitrogen) ...
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... Peptide – peptide interactions were detected using the SuperSignal West Pico PLUS chemiluminescent substrate (Thermo Fisher Scientific). The signals were acquired using the AI600 Imager (Amersham ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting peptide samples were desalted with a PierceTM Peptide Desalting Spin Column (Thermo Fisher Scientific) according to manufacturer’s instruction and then completely dried under speed-vacuum.